Methods of testing plastics. Sampling and test specimen preparation

2015 ◽  
Keyword(s):  
Test Specimen ◽  
Specimen Preparation

scholarly journals Enhancement of the AMPLICOR Enterovirus PCR Test with a Coprecipitant

1998 ◽  
Vol 36 (11) ◽  
pp. 3408-3409 ◽  
Author(s):  
E. William Taggart ◽  
Carrie L. Byington ◽  
David R. Hillyard ◽  
John E. Robison ◽  
Karen C. Carroll
Keyword(s):  
Test Specimen ◽  
False Negative ◽  
Blind Study ◽  
Specimen Preparation ◽  
Background Signal ◽  
Repeat Testing ◽  
Negative Results ◽  
Data Support ◽  
False Negative Results ◽  
Pcr Test

The incorporation of a commercially available coprecipitant into the AMPLICOR enterovirus PCR test specimen preparation enhanced the sensitivity and reproducibility of this assay. Fifty-five previously tested archived cerebrospinal fluids (CSF) specimens were tested in a blind study in duplicate with and without Pellet Paint coprecipitant (Novagen, Inc., Madison, Wis.). Of these specimens, 26 had previously been determined to be positive and 29 had previously been determined to be negative. All previously positive CSF specimens were positive when Pellet Paint was used and only 18 were positive without Pellet Paint. No previously negative specimens were positive on repeat testing with or without Pellet Paint. The background signal was not affected by the addition of Pellet Paint. These data support the utility of a coprecipitant in minimizing false-negative results.

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