The methods described were employed in the laboratories of the National Research Council for the examination of wool samples sent from the University of Alberta. The samples (as mentioned in Part I) were from sheep used to investigate the effect of various environmental factors on wool growth and quality.The determination of crimp, staple length, fibre length, tensile strength, elongation, fibre diameter, moisture content, wool wax, suint, dirt, and yield of clean wool is described. The methods for the measurement of fibre diameter and for the determination of wool wax, suint, dirt, and clean wool involved new technique and some novel features; they are, therefore, described more fully than the others.