Mismatch of screw thread systems

Microdensitometer—computer correlation analysis of ultrastructural periodicity

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081024 ◽

1978 ◽

Vol 36 (2) ◽

pp. 32-33

Author(s):

D.R. Ensor ◽

C.G. Jensen ◽

J.A. Fillery ◽

R.J.K. Baker

Keyword(s):

Correlation Analysis ◽

Background Noise ◽

Computer Control ◽

Optical Diffraction ◽

Screw Thread ◽

Straight Line ◽

Computer Storage ◽

Correlation Process ◽

Electron Microscope Image ◽

Fixed Beam

Because periodicity is a major indicator of structural organisation numerous methods have been devised to demonstrate periodicity masked by background “noise” in the electron microscope image (e.g. photographic image reinforcement, Markham et al, 1964; optical diffraction techniques, Horne, 1977; McIntosh,1974). Computer correlation analysis of a densitometer tracing provides another means of minimising "noise". The correlation process uncovers periodic information by cancelling random elements. The technique is easily executed, the results are readily interpreted and the computer removes tedium, lends accuracy and assists in impartiality.A scanning densitometer was adapted to allow computer control of the scan and to give direct computer storage of the data. A photographic transparency of the image to be scanned is mounted on a stage coupled directly to an accurate screw thread driven by a stepping motor. The stage is moved so that the fixed beam of the densitometer (which is directed normal to the transparency) traces a straight line along the structure of interest in the image.

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