scholarly journals Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard

2016 ◽  
Vol 6 (1) ◽  
pp. 30173 ◽  
Author(s):  
Helene Wahlström ◽  
Arianna Comin ◽  
Mats Isaksson ◽  
Peter Deplazes
Keyword(s):  
Sensitivity And Specificity ◽  
Echinococcus Multilocularis ◽  
Gold Standard ◽  
Diagnostic Sensitivity ◽  
Diagnostic Sensitivity And Specificity

scholarly journals Clinical significance of small molecule metabolites in the blood of patients with different types of liver injury

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hui Li ◽  
Yan Wang ◽  
Shizhao Ma ◽  
Chaoqun Zhang ◽  
Hua Liu ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Acid Amide ◽  
Hbv Infection ◽  
Diagnostic Sensitivity ◽  
Future Research ◽  
Diagnostic Efficacy ◽  
Metabolic Markers ◽  
Diagnostic Sensitivity And Specificity

AbstractTo understand the characteristic of changes of serum metabolites between healthy people and patients with hepatitis B virus (HBV) infection at different stages of disease, and to provide reference metabolomics information for clinical diagnosis of liver disease patients. 255 patients with different stages of HBV infection were selected. 3 mL blood was collected from each patient in the morning to detect differences in serum lysophosphatidylcholine, acetyl-l-carnitine, oleic acid amide, and glycocholic acid concentrations by UFLC-IT-TOF/MS. The diagnostic values of four metabolic substances were evaluated by receiver operating characteristic (ROC) curve. The results showed that the optimal cut-off value of oleic acid amide concentration of the liver cirrhosis and HCC groups was 23.6 mg/L, with a diagnostic sensitivity of 88.9% and specificity of 70.6%. The diagnostic efficacies of the three substances were similar in the hepatitis and HCC groups, with an optimal cut-off value of 2.04 mg/L, and a diagnostic sensitivity and specificity of 100% and 47.2%, respectively. The optimal cut-off value of lecithin of the HBV-carrier and HCC groups was 132.85 mg/L, with a diagnostic sensitivity and specificity of 88.9% and 66.7%, respectively. The optimal cut-off value of oleic acid amide of the healthy and HCC groups was 129.03 mg/L, with a diagnostic sensitivity and specificity of 88.4% and 83.3%, respectively. Lysophosphatidylcholine, acetyl-l-carnitine, and oleic acid amide were potential metabolic markers of HCC. Among them, lysophosphatidylcholine was low in the blood of HCC patients, and its diagnostic efficacy was better than that of acetyl-l-carnitine and oleic acid amide, providing reference metabolomics information in clinical diagnosis and future research.

scholarly journals Myo-Inositol Oxygenase as a Novel Marker in the Diagnosis of Acute Kidney Injury

2018 ◽  
Vol 37 (1) ◽  
pp. 1-6
Author(s):  
Cuma Mertoglu ◽  
Murat Gunay ◽  
Ali Gurel ◽  
Mehmet Gungor
Keyword(s):  
Acute Kidney Injury ◽  
Serum Creatinine ◽  
Sensitivity And Specificity ◽  
Cystatin C ◽  
Area Under The Curve ◽  
Kidney Injury ◽  
Diagnostic Sensitivity ◽  
Diagnostic Efficiency ◽  
Characteristic Analysis ◽  
Diagnostic Sensitivity And Specificity

SummaryBackground: Due to the lack of diagnostic efficiency of serum creatinine in acute kidney injury (AKI), there is a pressing need to develop novel diagnostic markers. Therefore, in this study, we evaluated myo-inositol oxygenase (MIOX), neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C in terms of their applicability in the diagnosis of AKI. Methods: We enrolled a total of 39 AKI patients and 38 healthy controls in the study. We compared the levels of serum MIOX, NGAL and cystatin C between the two groups. Results: We found that the concentrations of serum creatinine, blood-urea nitrogen, MIOX and cystatin C were higher in the AKI group. According to the receiver operating characteristic analysis, the area under the curve (AUC) values were 0.694 (95% CI 0.579-0.794) for MIOX and 0.976 (95% CI; 0.912-0.997) for cystatin C. For MIOX, when the cut-off concentration was set to 77.3 pg/mL, the diagnostic sensitivity and specificity were found to be 53.8% (95% CI; 37.2-69.9) and 81.5 (95% CI; 65.7-92.3), respectively. For cystatin C, at the cut-off value of 14 mg/L, the diagnostic sensitivity and specificity were 94.8% (95% CI; 82.7-99.4) and 94.7 % (95% CI 82.3-99.4), respectively. Conclusion: The measurement of serum MIOX and cystatin C levels is valuable for the diagnosis of AKI. Further research is needed for the evaluation of the potential use of MIOX as a kidney-specific enzyme in the early diagnosis of AKI.

Diagnosing coeliac disease by rectal gluten challenge: aprospective study based on immunopathology, computerized image analysisand logistic regression analysis

2001 ◽  
Vol 101 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Arzu ENSARI ◽  
Michael N. MARSH ◽  
Shethah MORGAN ◽  
Robert LOBLEY ◽  
D. J. UNSWORTH ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Coeliac Disease ◽  
Sensitivity And Specificity ◽  
Physiological Saline ◽  
Diagnostic Sensitivity ◽  
Cell Counts ◽  
Highly Sensitive ◽  
Endomysial Antibodies ◽  
Diagnostic Sensitivity And Specificity ◽  
Challenge Group

The purpose of this study was to evaluate the use of rectal gluten challenge in the diagnosis of coeliac disease. A total of 103 patients with features suggestive of this diagnosis were prospectively enrolled into the study; a diagnosis of coeliac disease was based on strictly defined criteria used in judging the proximal jejunal biopsy. On that basis, 45 out of the 103 patients were deemed to have coeliac disease. A slurry of gluten powder in physiological saline was introduced into the rectum, and biopsies taken before and at 2 h or 4 h after the challenge were examined immunohistochemically by computerized image analysis. Cell counts were analysed by logistic regression, and the best equations were obtained for each challenge group. The 2 h challenge yielded diagnostic sensitivity and specificity of 69.6% and 78.6% respectively. The 4 h challenge provided sensitivity and specificity of 100% and 100% respectively. These results were compared with other clinical diagnostic predictors, including anti-endomysial antibodies, which yielded diagnostic sensitivity and specificity of 70% and 98% respectively. It is concluded that a 4 h rectal challenge is a highly sensitive means of identifying gluten-sensitized individuals, and would be of particular value in cases showing negative antibody screening or equivocal biopsy appearances.

scholarly journals Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assays used in the UK

2011 ◽  
Vol 60 (11) ◽  
pp. 1598-1604 ◽  
Author(s):  
Rachel M. Chalmers ◽  
Brian M. Campbell ◽  
Nigel Crouch ◽  
André Charlett ◽  
Angharad P. Davies
Keyword(s):  
Sensitivity And Specificity ◽  
Diagnostic Sensitivity ◽  
Diagnostic Sensitivity And Specificity ◽  
The Uk
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