scholarly journals PEMERIKSAAN KLINIK BERBASIS BIOSENSOR BAGIAN 2: Biosensor Virus Untuk Deteksi Penyakit Patogen

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
NUR HABIBAH

Nowadays, rapid determination of several viruses which caused pathogen diseases is really important. Most of rapid detection of human pathogen viruses was developed by using biosensor technology. Biosensor technology offers several advantages, such as simple, efficient, low cost, fast response, easy to operate, and reliable. Viral detection by using biosensor can also avoid the delay of diagnosis, so the doctor can determine the type of drugs quickly and also can decide the type of patient care, properly. Most of biosensor for virus detection was exploited by using electrochemical principle, with amperometric and volumetric transducer. Almost of virus biosensor used immobilized antibody onto electrode surface as a biorecognition element. Some of viruses that could be detected by using electrochemical biosensor are HCV, HBV, HIV and influenza virus. However, quality control of the biosensor result is important, so the biosensor could be selected as an alternative method for on-site determination, especially in clinical determination. Keywords: biosensor, virus biosensor, virus detection, pathogen diseases detection

2019 ◽  
Vol 411 (28) ◽  
pp. 7539-7549 ◽  
Author(s):  
Quang Thuan Tran ◽  
Thi Tinh Phung ◽  
Quang Trung Nguyen ◽  
Truong Giang Le ◽  
Corinne Lagrost

Author(s):  
B. Radin ◽  
M. Shpitalni ◽  
I. Hartman

Abstract This paper presents an algorithm for solving the complex and critical problem of bending sequence in sheet metal manufacturing. Finding the bending sequence and required tool assignment presents a large combinatorial problem which is impossible to solve optimally for practical applications within a reasonable period of time. The paper presents a two-stage algorithm. The first stage finds a feasible solution based upon collision avoidance heuristics. The second stage rapidly seeks an alternative feasible sequence with a lower cost without exceeding time limitations. The algorithm is very practical because it reaches a low-cost solution quickly within computer memory limitations. In this paper, the problem is defined, the approach is presented formally, and finally, the power of the algorithm is demonstrated by solving bending sequences for real products.


2014 ◽  
Vol 3 (4) ◽  
pp. 101 ◽  
Author(s):  
Riccarda Antiochia ◽  
Lo Gorton ◽  
Luisa Mannina

<p>The aim of the present work was the development of a novel amperometric biosensor for rapid detection of sucrose in fruit juices samples. Two enzymes, invertase and fructose dehydrogenase (FDH), were immobilized onto a single-walled carbon nanotube paste (SWCNTP) electrode by wiring with a highly original osmium-polymer hydrogel. A second biosensor, for fructose only, was constructed containing inactive invertase and used for signal subtraction.</p> <p>The biosensor exhibits a detection limit for sucrose of 2 mM, linearity up to 5 mM, good sensitivity of 1.98 mA cm<sup>-2</sup> mM, good reproducibility (RSD = 2.5%), fast response time (8s) and a stability of 4 months if kept under wet conditions at 4 °C.</p> <p>The biosensor was successively tested for specific detection of sucrose and fructose in several commercial fruit juice samples and the results were compared with those obtained with a commercial spectrophotometric enzymatic kit.</p>


2017 ◽  
Vol 9 (15) ◽  
pp. 2305-2314 ◽  
Author(s):  
Nikola Sakač ◽  
Maja Karnaš ◽  
Marija Jozanović ◽  
Martina Medvidović-Kosanović ◽  
Sanja Martinez ◽  
...  

Long-life, low-cost and fast-response graphite pencil lead surfactant sensors based on MWCNTs.


2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
E. Durán ◽  
J. M. Andújar ◽  
J. M. Enrique ◽  
J. M. Pérez-Oria

A versatile measurement system for systematic testing and measurement of the evolution of theI-Vcharacteristic curves of photovoltaic panels or arrays (PV generators) is proposed in this paper. The measurement system uses a circuit solution based on DC-DC converters that involves several advantages relative to traditional methods: simple structure, scalability, fast response, and low cost. The measurement of the desired characteristics of PV generators includes high speed of response and high fidelity. The prototype system built is governed by a microcontroller, and experimental results prove the proposed measurement system useful. A virtual instrument (VI) was developed for full system control from a computer. The developed system enables monitoring the suitable operation of a PV generator in real time, since it allows comparing its actual curves with those provided by the manufacturer.


2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Rute C. Martins ◽  
Ana M. Pereira ◽  
Elisabete Matos ◽  
Luisa Barreiros ◽  
António J. M. Fonseca ◽  
...  

Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2′-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μg L−1 in sample acid digests, with a working range of 10 to 200 μg L−1, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μg L−1) and <11.7% (10 μg L−1). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.


Author(s):  
Xiuke Du ◽  
Song Ye ◽  
Daming Dong

Infrared spectroscopy is an effective method for the determination of compositions and concentrations of liquids, with advantages of fast response, no-sampling, flexible in use and is able for on-line monitoring. However, for trace substances in drinking water, such as nitrates and heavy metals, infrared spectroscopy is not sensitive enough for the quantitative and qualitative measurement. In this study, we improved the sensitivity of infrared spectroscopy for nitrite determination by developing an ion-exchange-enhanced diffuse reflectance spectroscopy, which consist of an accessory based on ion-exchange resin for enrichment and a FTIR spectrometer for measurement. Using this method, the limit of detection for nitrate is 1.7 mg/L, which is enough for drinking water sensing. We also verified the quantitative measurement ability of the method. Furthermore, the limit of detection and quantitative measuring range could be adjusted by changing the mass of resin and adsorption time. This study demonstrated the method can be used to detect trace nitrites in drinking water, can be applied in the field, and is sensitive, rapid, and inexpensive with a wide dynamic range.


2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaofeng Lin ◽  
Yanqiu Mei ◽  
Chen He ◽  
Yan Luo ◽  
Min Yang ◽  
...  

Escherichia coli (E. coli) O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of E. coli O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases. In this study, a carbon dots-Fe3O4 nanomaterial (CDs-Fe3O4)-based sensitive electrochemical biosensor for E. coli O157:H7 detection was developed. The CDs have good electrical conductivity, and the surface of carbon dots contains abundant carboxyl groups, which can be used to immobilize probe DNA. Meanwhile, the CDs can be used as a reducing agent to prepare CDs-Fe3O4 nanomaterial. The Fe3O4 nanomaterial can improve the performance of the electrochemical biosensor; it also can realize the recovery of CDs-Fe3O4 due to its magnetism. As expected, the electrochemical biosensor has excellent specificity of E. coli O157:H7 among other bacteria. The electrochemical biosensor also exhibited good performance for detecting E. coli O157:H7 with the detection range of 10–108 CFU/ml, and the detection limit of this electrochemical biosensor was 6.88 CFU/ml (3S/N). Furthermore, this electrochemical biosensor was successfully used for monitoring E. coli O157:H7 in milk and water samples, indicating that this electrochemical biosensor has good application prospect. More importantly, this research can provide a new idea for the detection of other bacteria and viruses.


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