scholarly journals Streptococcal collagen-like protein 1, Scl1, modulates group a Streptococcus adhesion, biofilm formation and virulence

2016 ◽  
Author(s):  
Beth Alexandra Bachert
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Group A

scholarly journals Biofilm formation by group A Streptococcus: a role for the streptococcal regulator of virulence (Srv) and streptococcal cysteine protease (SpeB)

Microbiology ◽  
2009 ◽  
Vol 155 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Christopher D. Doern ◽  
Amity L. Roberts ◽  
Wenzhou Hong ◽  
Jessica Nelson ◽  
Slawomir Lukomski ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Immunoblot Analysis ◽  
Wild Type ◽  
Flow Conditions ◽  
Group A ◽  
Streptococcus A ◽  
Insight Into ◽  
Western Immunoblot Analysis

Recently, biofilms have become a topic of interest in the study of the human pathogen group A Streptococcus (GAS). In this study, we sought to learn more about the make-up of these structures and gain insight into biofilm regulation. Enzymic studies indicated that biofilm formation by GAS strain MGAS5005 required an extracellular protein and DNA component(s). Previous results indicated that inactivation of the transcriptional regulator Srv in MGAS5005 resulted in a significant decrease in virulence. Here, inactivation of Srv also resulted in a significant decrease in biofilm formation under both static and flow conditions. Given that production of the extracellular cysteine protease SpeB is increased in the srv mutant, we tested the hypothesis that increased levels of active SpeB may be responsible for the reduction in biofilm formation. Western immunoblot analysis indicated that SpeB was absent from MGAS5005 biofilms. Complementation of MGAS5005Δsrv restored the biofilm phenotype and eliminated the overproduction of active SpeB. Inhibition of SpeB with E64 also restored the MGAS5005Δsrv biofilm to wild-type levels.

scholarly journals Assessing the Role of Pharyngeal Cell Surface Glycans in Group A Streptococcus Biofilm Formation

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 775
Author(s):  
Heema K. N. Vyas ◽  
Anuk D. Indraratna ◽  
Arun Everest-Dass ◽  
Nicolle H. Packer ◽  
David M. P. De Oliveira ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Treatment Failure ◽  
Antibiotic Treatment ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Group A Streptococcus ◽  
Sialic Acid Residue ◽  
Group A ◽  
Terminal Mannose

Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Antibiotic treatment failure rates of 20–40% have been observed. The role host cell glycans play in GAS biofilm formation in the context of GAS pharyngitis and subsequent antibiotic treatment failure has not been previously investigated. GAS serotype M12 GAS biofilms were assessed for biofilm formation on Detroit 562 pharyngeal cell monolayers following enzymatic removal of all N-linked glycans from pharyngeal cells with PNGase F. Removal of N-linked glycans resulted in an increase in biofilm biomass compared to untreated controls. Further investigation into the removal of terminal mannose and sialic acid residues with α1-6 mannosidase and the broad specificity sialidase (Sialidase A) also found that biofilm biomass increased significantly when compared to untreated controls. Increases in biofilm biomass were associated with increased production of extracellular polymeric substances (EPS). Furthermore, it was found that M12 GAS biofilms grown on untreated pharyngeal monolayers exhibited a 2500-fold increase in penicillin tolerance compared to planktonic GAS. Pre-treatment of monolayers with exoglycosidases resulted in a further doubling of penicillin tolerance in resultant biofilms. Lastly, an additional eight GAS emm-types were assessed for biofilm formation in response to terminal mannose and sialic acid residue removal. As seen for M12, biofilm biomass on monolayers increased following removal of terminal mannose and sialic acid residues. Collectively, these data demonstrate that pharyngeal cell surface glycan structures directly impact GAS biofilm formation in a strain and glycan specific fashion.

scholarly journals Characterization of Biofilm Formation by Clinically Relevant Serotypes of Group A Streptococci

2006 ◽  
Vol 72 (4) ◽  
pp. 2864-2875 ◽  
Author(s):  
Cordula Lembke ◽  
Andreas Podbielski ◽  
Carlos Hidalgo-Grass ◽  
Ludwig Jonas ◽  
Emanuel Hanski ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Laser Scanning ◽  
Group A Streptococcus ◽  
Three Dimensional ◽  
Positive Control ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Subsequent Formation ◽  
Group A

ABSTRACT Streptococcus pyogenes (group A streptococcus [GAS]) is a frequent cause of purulent infections in humans. As potentially important aspects of its pathogenicity, GAS was recently shown to aggregate, form intratissue microcolonies, and potentially participate in multispecies biofilms. In this study, we show that GAS in fact forms monospecies biofilms in vitro, and we analyze the basic parameters of S. pyogenes in vitro biofilm formation, using Streptococcus epidermidis as a biofilm-positive control. Of nine clinically important serotype strains, M2, M6, M14, and M18 were found to significantly adhere to coated and uncoated polystyrene surfaces. Fibronectin and collagen types I and IV best supported primary adherence of serotype M2 and M18 strains, respectively, whereas serotype M6 and M14 strains strongly bound to uncoated polystyrene surfaces. Absorption measurements of safranin staining, as well as electron scanning and confocal laser scanning microscopy, documented that primary adherence led to subsequent formation of three-dimensional biofilm structures consisting of up to 46 bacterial layers. Of note, GAS isolates belonging to the same serotype were found to be very heterogeneous in their biofilm-forming behavior. Biofilm formation was equally efficient under static and continuous flow conditions and consisted of the classical three steps, including partial disintegration after long-term incubation. Activity of the SilC signaling peptide as a component of a putative quorum-sensing system was found to influence the biofilm structure and density of serotype M14 and M18 strains. Based on the presented methods and results, standardized analyses of GAS biofilms and their impact on GAS pathogenicity are now feasible.

scholarly journals Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus

2016 ◽  
Vol 6 ◽  
Author(s):  
Beth A. Bachert ◽  
Soo J. Choi ◽  
Paul R. LaSala ◽  
Tiffany I. Harper ◽  
Dudley H. McNitt ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Type Group ◽  
Group A

scholarly journals Cellular chaining influences biofilm formation and structure in group A Streptococcus

Biofilm ◽  
2020 ◽  
Vol 2 ◽  
pp. 100013
Author(s):  
Artur Matysik ◽  
Foo Kiong Ho ◽  
Alicia Qian Ler Tan ◽  
Anuradha Vajjala ◽  
Kimberly A. Kline
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Group A

The nutritional requirements for biofilm formation by Group A streptococcus

2006 ◽  
Vol 1289 ◽  
pp. 139-142 ◽  
Author(s):  
Josephine Shera ◽  
Kadaba S. Sriprakash ◽  
David J. McMillan
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Nutritional Requirements ◽  
Group A

scholarly journals Cellular chaining influences biofilm formation and structure in Group A Streptococcus

2019 ◽  
Author(s):  
Artur Matysik ◽  
Foo Kiong Ho ◽  
Alicia Qian Ler Tan ◽  
Anuradha Vajjala ◽  
Kimberly A. Kline
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Pathological Feature ◽  
Host Environment ◽  
Group A ◽  
Chain Lengths

ABSTRACTGroup A Streptococcal (GAS) biofilm formation is an important pathological feature contributing to the antibiotic tolerance and progression of various GAS infections. Although a number of bacterial factors have been described to promote in vitro GAS biofilm formation, the relevance of in vitro biofilms to host-associated biofilms requires further understanding. In this study, we demonstrate how constituents of the host environment, such as lysozyme and NaCl, can modulate GAS bacterial chain length and, in turn, shape GAS biofilm morphology and structure. Disruption of GAS chains with lysozyme results in biofilms that are more stable. Based on confocal microscopy, we attribute the increase in biofilm stability to a dense and compact three-dimensional structure produced by de-chained cells. To show that changes in biofilm stability and structure are due to the shortening of bacterial chains and not specific to the activity of lysozyme, we demonstrate that augmented chaining induced by NaCl or deletion of the autolysin gene mur1.2 produced defects in biofilm formation characterized by a loose biofilm architecture. We conclude that GAS biofilm formation can be directly influenced by host and environmental factors through the modulation of bacterial chain lengths, potentially contributing to persistence and colonization within the host. Further studies of in vitro biofilm models incorporating physiological constituents such as lysozyme may uncover new insights into the physiology of in vivo GAS biofilms.

scholarly journals The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus

2011 ◽  
Vol 11 (1) ◽  
pp. 262 ◽  
Author(s):  
Heaven A Oliver-Kozup ◽  
Meenal Elliott ◽  
Beth A Bachert ◽  
Karen H Martin ◽  
Sean D Reid ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Group A Streptococcus ◽  
Significant Determinant ◽  
Group A
Sign in / Sign up

Export Citation Format

Share Document