scholarly journals Real-Time Behavior of a Microalgae–Bacteria Consortium Treating Wastewater in a Sequencing Batch Reactor in Response to Feeding Time and Agitation Mode

Water ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1893
Author(s):  
Emna Mhedhbi ◽  
Nadia Khelifi ◽  
Paola Foladori ◽  
Issam Smaali

A study of a microalgae–bacteria treatment system was conducted in a sequencing batch reactor (SBR) by combining a precultured native algae Nannochloropsis gaditana L2 with spontaneous municipal wastewater microorganisms. Two types of agitation, air mixing (AI) and mechanical mixing (MIX), were assessed at continuous illumination (L) and photoperiod cycle light/dark (L/D). The obtained consortium, via native microalgae addition, has a better operational efficiency compared to spontaneous control. This allows the removal of 78% and 53% of total Kjeldhal nitrogen (TKN) and chemical oxygen demand (COD), respectively. Under the (L/D) photoperiod, the optimal removal rate (90% of TKN and 75% of COD) was obtained by the consortium at 4 days of hydraulic retention time (HRT) using the AI mode. Moreover, during feeding during dark (D/L) photoperiod, the highest removal rate (83% TKN and 82% COD) was recorded at 4 days HRT using the AI mode. These results bring, at the scale of a bioreactor, new data regarding the mode of aeration and the feeding time. They prove the concept of such a technology, increasing the attraction of microalgae-based wastewater treatment.

2011 ◽  
Vol 374-377 ◽  
pp. 1013-1016
Author(s):  
Hui Yang ◽  
Yu Zhang ◽  
Yue Xu

Abstract. The paper aims to study the fast start-up of anaerobic-aerobic-anoxic-aerobic sequencing batch reactor, with domestic sewage as treating object, to solve the problem of SBR that can be used for denitrification or dephosphorization independently and to realize simultaneous nitrogen and phosphorus removal in a single SBR system. Phosphorus accumulating organisms were enriched at the anaerobic condition for 2h/aerobic for 3h after activated sludge were inoculated. Then denitrifying polyphosphate-accumulating organisms were enriched by inserting an anoxic phase into the aerobic phase. The lengths of anaerobic time, anoxic time and aerobic time were adjusted and the nitrogen and phosphorus removal effect of (AO)2SBR system were observed. The (AO)2SBR system was started successfully with 80d of training and domesticating. The nitrogen and phosphorus removal effect was performed preferably at the condition of anaerobic(2h)-aerobic(1.5h)-anoxic(1.5h)-aerobic(0.5h). The removal rate of COD, NH4+-N, TN and TP reached 90%, 97%, 88% and 92% respectively. And 33% of energy was saved when aerobic time was shortened from 3h to 2h, while the treating effect dropped off rarely. The results show that (AO)2SBR is applicable for simultaneous nitrogen and phosphorus removal, and the effluent water quality meets the first level B criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant(GB 18918-2002). The system can also reach the aim of saving energy and providing theoretical basis for the nitrogen and phosphorus removal in single SBR systems.


2018 ◽  
Vol 78 (8) ◽  
pp. 1726-1732 ◽  
Author(s):  
S. Petrini ◽  
P. Foladori ◽  
G. Andreottola

Abstract Engineered microalgal-bacteria consortia are an attractive solution towards a low-cost and sustainable wastewater treatment that does not rely on artificial mechanical aeration. In the research conducted for this study, a bench-scale photo-sequencing-batch reactor (PSBR) was operated without external aeration. A spontaneous consortium of microalgae and bacteria was developed in the PSBR at a concentration of 0.8–1.7 g TSS/L. The PSBR ensured removal efficiency of 85 ± 8% for chemical oxygen demand (COD) and 98 ± 2% for total Kjeldahl nitrogen (TKN). Nitrogen balance revealed that the main mechanisms for TKN removal was autotrophic nitrification, while N assimilation and denitrification accounted for 4% and 56%, respectively. The development of dense microalgae–bacteria bioflocs resulted in good settleability with average effluent concentration of 16 mgTSS/L. The ammonium removal rate was 2.9 mgN L−1 h−1, which corresponded to 2.4 mgN gTSS−1 h−1. Although this specific ammonium removal rate is similar to activated sludge, the volumetric rate is lower due to the limited total suspended solids (TSS) concentration (three times less than activated sludge). Therefore, the PSBR footprint appears less competitive than activated sludge. However, ammonium was completely removed without artificial aeration, resulting in a very cost-effective process. Only 50% of phosphorus was removed, suggesting that further research on P uptake is needed.


2011 ◽  
Vol 233-235 ◽  
pp. 600-603
Author(s):  
Gao Shun Qiu ◽  
Ling Feng Qiu ◽  
Yi Ming Chen

The influences of HRT, C/N ratio on simultaneous nitrification and denitrification (SND) and the rule of pH in a sequencing batch reactor (SBR) were investigated while treating low C/N synthetic municipal wastewater. The results showed that the function of SND became more outstanding and the removal rate of TN, NH4+-N was improved greatly by lengthening HRT appropriately; when the C/N increased, the denitrification effect of the simultaneous nitrification and denitrification would be improved; .At the same time, the variation of pH value was well related to ammonia oxidation. So that judgment on the ending of nitrification and denitrification could be based on the inflection point on the varied curve of pH, and thus reducing aeration and mixing time for the purpose of energy saving.


2019 ◽  
Vol 6 (9) ◽  
pp. 190304 ◽  
Author(s):  
Liangang Hou ◽  
Jun Li ◽  
Zhaoming Zheng ◽  
Qi Sun ◽  
Yitao Liu ◽  
...  

The river sediment contains a lot of pollutants in many cases, and needs to be treated appropriately for the restoration of water environments. In this study, a novel method was developed to convert river sediment into denitrifying sludge in a sequencing batch reactor (SBR). The river sediment was added into the reactor daily and the hydraulic retention time (HRT) of the reactor was gradually reduced from 8 to 4 h. The reactor achieved in the N O 3   – N removal efficiency of 85% with the N O 3   – N removal rate of 0.27 kg N m −3 d −1 . Response surface analysis represents that nitrate removal was affected mainly by HRT, followed by sediment addition. The denitrifying sludge achieved the highest activity with the following conditions: N O 3   – N 50 mg l −1 , HRT 6 h and adding 6 ml river sediments to 1 l wastewater of reactor per day. As a result, the cultivated denitrifying sludge could remove 80% N O 3   – N for real municipal wastewater, and the high-throughput sequence analysis indicated that major denitrifying bacteria genera and the relative abundance in the cultivated denitrifying sludge were Diaphorobacter (33.82%) and Paracoccus (24.49%). The river sediments cultivating method in this report can not only obtain denitrifying sludge, but also make use of sediment resources, which has great application potential.


2013 ◽  
Vol 830 ◽  
pp. 481-484
Author(s):  
De Ying Mu

With synthetic domestic wastewater, denitrifying phosphorus accumulating organism was enriched and cultured by using activated sludge from a wastewater treatment plant in a set of sequencing batch reactor. Results showed that the system had a good effluent performance under the three-phase cultivation of anaerobic/aerobic, anaerobic/anoxic/aerobic and anaerobic/anoxic; the effluent concentration of chemical oxygen demand (COD) was below 25 mg/L, the average removal rate of phosphorus was 84.63%-88.19%.


2019 ◽  
Vol 1 (2) ◽  
pp. 1
Author(s):  
Lindawati Lindawati

Sebuah Sequencing Batch Reactor (SBR) digunakan untuk mengevaluasi peranan Biochemical Oxygen Demand (BOD) biosensor dalam proses optimasi proses pengolahan nutrien karbon, nitrogen dan fosfat. Hasil penelitian menunjukkan bahwa BOD biosensor dapat dipergunakan untuk penentuan karbon organik, sehingga reduksi siklus SBR dapat dilakukan dan efisiensi proses meningkat. Pola konsumsi karbon organik ditemukan dengan adanya ‘tanda diam’ pada fase anoksik/ anaerobik, di mana dari tanda ini, fase aerobik dapat segera dimulai. Reduksi durasi siklus SBR dari 8 jam menjadi 4 jam meningkatkan efiesiensi pengolahan C, N dan P yang meningkat pula (hampir dua kali lebih tinggi).


1994 ◽  
Vol 30 (6) ◽  
pp. 237-246 ◽  
Author(s):  
A. Carucci ◽  
M. Majone ◽  
R. Ramadori ◽  
S. Rossetti

This paper describes a lab-scale experimentation carried out to study enhanced biological phosphate removal (EBPR) in a sequencing batch reactor (SBR). The synthetic feed used was based on peptone and glucose as organic substrate to simulate the readily biodegradable fraction of a municipal wastewater (Wentzel et al., 1991). The experimental work was divided into two runs, each characterized by different operating conditions. The phosphorus removal efficiency was considerably higher in the absence of competition for organic substrate between P-accumulating and denitrifying bacteria. The activated sludge consisted mainly of peculiar microorganisms recently described by Cech and Hartman (1990) and called “G bacteria”. The results obtained seem to be inconsistent with the general assumption that the G bacteria are characterized by anaerobic substrate uptake not connected with any polyphosphate metabolism. Supplementary anaerobic batch tests utilizing glucose, peptone and acetate as organic substrates show that the role of acetate in the biochemical mechanisms promoting EBPR may not be so essential as it has been assumed till now.


2015 ◽  
Vol 73 (4) ◽  
pp. 740-745 ◽  
Author(s):  
Jan Dries

On-line control of the biological treatment process is an innovative tool to cope with variable concentrations of chemical oxygen demand and nutrients in industrial wastewater. In the present study we implemented a simple dynamic control strategy for nutrient-removal in a sequencing batch reactor (SBR) treating variable tank truck cleaning wastewater. The control system was based on derived signals from two low-cost and robust sensors that are very common in activated sludge plants, i.e. oxidation reduction potential (ORP) and dissolved oxygen. The amount of wastewater fed during anoxic filling phases, and the number of filling phases in the SBR cycle, were determined by the appearance of the ‘nitrate knee’ in the profile of the ORP. The phase length of the subsequent aerobic phases was controlled by the oxygen uptake rate measured online in the reactor. As a result, the sludge loading rate (F/M ratio), the volume exchange rate and the SBR cycle length adapted dynamically to the activity of the activated sludge and the actual characteristics of the wastewater, without affecting the final effluent quality.


Vestnik MGSU ◽  
2019 ◽  
pp. 589-602 ◽  
Author(s):  
Tran Ha Quan ◽  
Elena S. Gogina

Introduction. Vietnamese urban municipal wastewater treatment plants are mainly of aeration-type facilities. Nowadays, an aeration-type plant, the Sequencing Batch Reactor (SBR), is widely applied and possesses a number of advantages over traditional systems with suspended activated sludge. Advantages of the SBR are mainly concluded in simplicity of operation, occupied area and cost. There is a number of problems at the wastewater treatment plants; they are connected with supplying only a half of wastewater design amount for the treatment as well as with quality of the purified water that must satisfy requirements of the Vietnamese discharge standard, the Standard A. Therefore, reconstruction and modification of the SBR is the major challenger to ensure the sustained development of large Vietnamese cities and maintenance of ecological balance. Materials and methods. To enhance the efficiency of wastewater purification in the SBR, the experiments were set on reactor reconstruction and modification by two directions: (1) Technological method, i.e. applying the Biochip 25 biocarrier, and (2) Operation method, i.e. adding the anoxic phase in reactor operation cycle. Laboratory tests were conducted for each of the directions, including comparison of a typical reactor with the modified one. Results. The study resulted in obtaining an optimal amount of the BioChip biocarrier material (10 to 20 %) that increased efficiency of wastewater purification by 10 to 20 %. In addition to this, when creating an anoxic phase of the operation cycle, efficiency of nitrogen removal increased by 20 %. When the denitrification occurs under the anoxic conditions, it contributes to stabilization of ammonium nitrogen removal for daily nitrogen loading in reactor of 0.3 to 0.8 TKN kg/sludge kg. Conclusions. The suggested technology provides the quality of treated water corresponding with the Vietnamese Standard A requirements. At the present, it is planned to proceed with the experiment on the base of Vietnamese semi-industrial plant for research and appraisal of the SBR reconstruction and modification method. Acknowledgements. The authors are grateful to AKVA Control company in Samara for granted biocarrier Mutag BioChip 25 and to Associate Professor Tran Van Quang and his students, Nguyen Ngoc Phuong and Truong Quoc Dai, of Environment Protect Research Center, Danang University for support of the experiment.


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