scholarly journals Effects of Ca2+ Concentration on Anaerobic Ammonium Oxidation Reactor Microbial Community Structure

Water ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1341 ◽  
Author(s):  
Ma ◽  
Jin ◽  
Zhang
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Anaerobic Ammonium Oxidation ◽  
Microbiological Analysis ◽  
Ammonium Oxidation ◽  
Total N ◽  
Treatment Performance ◽  
N Removal ◽  
Oxidation Reactor

The anaerobic ammonium oxidation (anammox) reaction removes nitrogen from wastewater, the performance of which is influenced by Ca2+; however, the effect of Ca2+ on microbial community structure is unclear. Therefore, the effects of Ca2+ concentration on the treatment performance of an anammox reactor and microbial community structure of anammox sludge were investigated. Ca2+ concentration minimally influenced the removal efficiency of NO2−–N and NH4+–N, but substantially influenced total N removal. Changing the Ca2+ concentration (between 25 and 125 mg/L) caused the average removal rate of total nitrogen to fluctuate by 3.3 percentage points. There were five major bacterial phyla in the anammox sludge: Proteobacteria, Chloroflexi, Acidobacteria, Planctomycete, and Chlorobi. Microbiological analysis revealed that the genera Acidobacterium, Anaerolinea, and Denitratisoma were positively correlated with Ca2+ concentration, and improved treatment performance of the anammox reactor. Moreover, uncultured Chlorobi bacterium clone RUGL1-218 (GQ421108.1) and uncultured sludge bacterium A21b (KT182572.1) may be key microorganisms for the immobilization of anammox bacteria. These findings offer a theoretical basis for improved wastewater treatment using the anammox process.

scholarly journals Effect of Aeration Rates and Filter Media Heights on the Performance of Pollutant Removal in an Up-Flow Biological Aerated Filter

Water ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1244 ◽  
Author(s):  
Jiehui Ren ◽  
Wen Cheng ◽  
Tian Wan ◽  
Min Wang ◽  
Chengcheng Zhang
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Relative Abundance ◽  
Microbial Community Structure ◽  
Municipal Wastewater ◽  
Biological Aerated Filter ◽  
Filter Media ◽  
N Removal ◽  
Pollutants Removal ◽  
Aerated Filter

The biological aerated filter (BAF) is an effective biological treatment technology which removes the pollutants in municipal wastewater secondary treatment. However, we still know little about the interaction between the pollutants removal and microbes within the BAF. In this study, we used an up-flow BAF (UBAF) reactor to investigate the relationships between the pollutants removal and microbial community structure at different aeration rates and filter media heights. The microbial community of biofilm was analyzed by Illumina pyrosequencing. Our results showed that the UBAF achieved a better removal efficiency of chemical oxygen demand (COD), NH4+-N, NO3−-N, and total phosphorus (TP) at an aeration rate of 65 L/h. In addition, the COD and NH4+-N removal mainly occurred at 0–25 cm height of filter media. The microbial community structure in the UBAF demonstrated that the relative abundance of the Planctomycetes and Comamonadaceae at 10 cm height of filter media were 11% and 48.1%, respectively, proportions significantly higher than those under others treatments. Finally, the changes in relative abundance of Proteobacteria, Planctomycetes, and Nitrospirae likely explained the mechanism of nitrogen and phosphorus removal. Our results showed that suitable conditions could enhance the microbial community structure to achieve a high pollutants removal in the UBAF.

scholarly journals Nitrogen Retention in Mesocosm Sediments Received Rural Wastewater Associated with Microbial Community Response to Plant Species

Water ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3035
Author(s):  
Zhixin Dong ◽  
Lei Hu ◽  
Jianmei Li ◽  
Mathieu Nsenga Kumwimba ◽  
Jialiang Tang ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Plant Species ◽  
Microbial Community Structure ◽  
Ammonia Oxidation ◽  
Community Response ◽  
Nitrifying Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
N Removal ◽  
Rural Wastewater

Vegetated drainage ditches (eco-ditches) have drawn much attention in recent years for the ability to remediate diffuse contaminants in rural wastewater through sediment retention, plant uptake and interception, and microbial metabolic activities. However, the effect of plant species on microbial community structure and nitrogen (N) retention in ditch sediment remains poorly understood. In this study, mesocosm plastic drums were planted with eight plant species commonly found in ditches and nurtured with wastewater for 150 days. Sediment total nitrogen (TN) was greatly increased after 150-day nurturing with rural wastewater, from 296.03 mg∙kg−1 (Iris japonica Thunb) to 607.88 mg∙kg−1 (Acorus gramineusO). This study also presents the effect of different plant species on sediment microbial communities, thus providing insight into N removal mechanisms in eco-ditch. Fifty-eight differentially abundant taxa were identified, and sediment microbial community structure for no plant (CK), Acg, Canna indica (Cai), and Typha latifolia L. (Tyl) was primarily linked to sediment NH4+-N and TN. Extremely small proportions of ammonia oxidizing bacteria (AOB) and nitrifying bacteria were detected for all treatments, but large proportions of Crenarchaeota, which comprises the widely existent ammonium oxidized archaea (AOA), were found in CK, Acg and Cai. The abundance of Nitrosotalea from Crenarchaeota presented positive correlations with sediment NH4+-N contents and ammonia oxidation function predicted by Faprotax, indicating Nitrosotalea might be the dominant ammonium-oxidizing microbes in sediment samples. The probable NH4+-N removal pathway in wastewater sediment was through a combined effect of AOA, nitrifying bacteria, and anammox.

scholarly journals Sesuvium portulacastrum-Mediated Removal of Nitrogen and Phosphorus Affected by Sulfadiazine in Aquaculture Wastewater

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 68
Author(s):  
Chaoyue Zhang ◽  
Dan Wang ◽  
Weihong He ◽  
Hong Liu ◽  
Jianjun Chen ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Antibiotic Residues ◽  
Sesuvium Portulacastrum ◽  
Nitrogen And Phosphorus ◽  
Removal Rates ◽  
N Removal ◽  
Aquaculture Wastewater ◽  
P Removal

Plant-based removal of nitrogen (N) and phosphorus (P) from water bodies is an important method for remediation of aquaculture wastewater. In order to acquire knowledge as to how antibiotic residues in wastewater might affect the microbial community and plant uptake of N and P, this study investigated N and P removal by a coastal plant Sesuvium portulacastrum L. grown in aquaculture wastewater treated with 0, 1, 5, or 50 mg/L sulfonamide antibiotics (sulfadiazine, SD) for 28 days and compared the microbial community structure between the water and rhizosphere. Results showed that SD significantly decreased N removal rates from 87.5% to 22.1% and total P removal rates from 99.6% to 85.5%. Plant fresh weights, root numbers, and moisture contents as well as activities of some enzymes in leaves were also reduced. SD changed the microbial community structure in water, but the microbial community structure in the rhizosphere was less affected by SD. The microbial diversity in water was higher than that in the rhizosphere, indicating microbial community differences. Our results showed that the commonly used antibiotic, SD, in aquaculture can inhibit plant growth, change the structure of microbial community, and reduce the capacity of S. portulacastrum plants to remove N and P from wastewater, and also raised alarm about detrimental effects of antibiotic residues in phytoremediation of wastewater.

scholarly journals Nitrogen Removal Characteristics and Comparison of the Microbial Community Structure in Different Anaerobic Ammonia Oxidation Reactors

Water ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 230 ◽  
Author(s):  
Liqiu Zhang ◽  
Wei Lv ◽  
Shugeng Li ◽  
Zhongxuan Geng ◽  
Hainan Yao
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Nitrogen Removal ◽  
Microbial Community Structure ◽  
Ammonia Oxidation ◽  
Settling Tank ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Start Up ◽  
Anaerobic Ammonia Oxidation

Nitrogen removal characteristics and the comparison of the microbial community structure were investigated in different anaerobic ammonia oxidation (Anammox) reactors: an anaerobic sequencing batch reactor (ASBR) and a biofilter reactor. The Anammox systems were inoculated with sludge from the second settling tank of a wastewater treatment plant in Guangzhou, China. After successful start up of Anammox, the microbial community structure of different Anammox reactors were studied through high-throughput sequencing. The results showed that anaerobic ammonium oxidation in the ASBR reactor could successfully start up after 134 days, while Anammox in the biofilter could start up after 114 days. In both systems, total nitrogen removal was at 80% after more than 200 days of operation. The diversity of denitrifying microorganisms was high in both reactors, with Planctomycetes as the main taxa. Anammox bacteria belonging to the genera Candidatus Anammoxoglobus and Kuenenia, were dominant in the ASBR, while all three genera of Candidatus, Anammoxoglobus, Kuenenia, and Brocadia, could be detected in the biofilter reactor. Therefore, the biofilter starts up faster than the ASBR, and contains richer species, which makes it more suitable to domesticate Anammox bacteria.

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