scholarly journals Developing a Biotechnological Tool for Monitoring Water Quality: In vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica

Water ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 145 ◽  
Author(s):  
S. Debén ◽  
J.R. Aboal ◽  
P. Giráldez ◽  
Z. Varela ◽  
J.A. Fernández
Keyword(s):  
Water Quality ◽  
Growth Rate ◽  
Large Scale ◽  
N:P Ratio ◽  
Medium Composition ◽  
Fontinalis Antipyretica ◽  
Active Biomonitoring ◽  
Aquatic Moss ◽  
Aquatic Mosses

One of the main factors limiting active biomonitoring with aquatic mosses is the lack of sufficient material. A laboratory culture of the moss would solve this problem and thus convert the technique into a valuable biotechnological tool for monitoring water quality. With this aim, we first established small and large scale axenic in vitro culture systems for the aquatic moss Fontinalis Antipyretica. We then attempted to enhance the growth rate of the cultures by modifying temperature, photoperiod and medium composition (N:P ratio, P concentration, CO2 supply, NH4NO3 supply and sucrose supply). None of these modifications greatly increased the in vitro growth rate. However, the growth rates were sufficiently high (relative to the initial weight of the cultures) in both systems (45 and 6 mg·day−1·g−1· for flasks and bioreactors respectively) to enable the production of large amounts of material. The ability to culture the material will substantially improve the applicability of the moss bag technique.

scholarly journals Effect of Current Velocity on Cd Accumulation in the Aquatic Moss Fontinalis antipyretica

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 157
Author(s):  
Antón Vázquez-Arias ◽  
Sofía Debén ◽  
Adrián Casanova ◽  
Carme Pacín ◽  
Jesús R. Aboal ◽  
...  
Keyword(s):  
Regression Model ◽  
Water Flow ◽  
Current Velocity ◽  
Aquatic Environment ◽  
Water Velocity ◽  
Fontinalis Antipyretica ◽  
Aquatic Moss ◽  
Cd Accumulation ◽  
Aquatic Mosses ◽  
The Relationship

With the aim of further standardizing biomonitoring techniques with aquatic mosses, the relationship between the velocity of water flow and cadmium (Cd) accumulation in transplants of the moss Fontinalis antipyretica was investigated. For this purpose, moss transplants were exposed in a controlled aquatic environment to different concentrations of Cd (0, 4, 16 and 36 ng g−1) and different water velocities (10, 30, 50, 70 and 90 cm s−1). The Cd concentrations in the moss transplants mainly depended on the Cd concentration in the water, but a small fraction of the variance was explained by water velocity. The Cd concentrations in moss were standardized to remove the effect of the concentration in the water so all the data could be analyzed together. The regression model for the standardized concentrations explained 23% of the variance in Cd accumulation in F. antipyretica and water velocity proved to be a significant predictor of Cd accumulation.

scholarly journals A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays

2016 ◽  
Vol 82 (18) ◽  
pp. 5553-5562 ◽  
Author(s):  
Hannah B. Pooley ◽  
Kumudika de Silva ◽  
Auriol C. Purdie ◽  
Douglas J. Begg ◽  
Richard J. Whittington ◽  
...  
Keyword(s):  
Growth Rate ◽  
Quantitative Pcr ◽  
Mycobacterium Avium ◽  
Large Scale ◽  
Liquid Culture ◽  
Vaccine Development ◽  
Culture Method ◽  
Content Type ◽  
Antimicrobial Screening

ABSTRACTDetermining the viability of bacteria is a key outcome ofin vitrocellular infection assays. Currently, this is done by culture, which is problematic for fastidious slow-growing bacteria such asMycobacterium aviumsubsp.paratuberculosis, where it can take up to 4 months to confirm growth. This study aimed to identify an assay that can rapidly quantify the number of viableM. aviumsubsp.paratuberculosiscells in a cellular sample. Three commercially available bacterial viability assays along with a modified liquid culture method coupled with high-throughput quantitative PCR growth detection were assessed. Criteria for assessment included the ability of each assay to differentiate live and deadM. aviumsubsp.paratuberculosisorganisms and their accuracy at low bacterial concentrations. Using the culture-based method,M. aviumsubsp.paratuberculosisgrowth was reliably detected and quantified within 2 weeks. There was a strong linear association between the 2-week growth rate and the initial inoculum concentration. The number of viableM. aviumsubsp.paratuberculosiscells in an unknown sample was quantified based on the growth rate, by using growth standards. In contrast, none of the commercially available viability assays were suitable for use with samples fromin vitrocellular infection assays.IMPORTANCERapid quantification of the viability ofMycobacterium aviumsubsp.paratuberculosisin samples fromin vitrocellular infection assays is important, as it allows these assays to be carried out on a large scale.In vitrocellular infection assays can function as a preliminary screening tool, for vaccine development or antimicrobial screening, and also to extend findings derived from experimental animal trials. Currently, by using culture, it takes up to 4 months to obtain quantifiable results regardingM. aviumsubsp.paratuberculosisviability after anin vitroinfection assay; however, with the quantitative PCR and liquid culture method developed, reliable results can be obtained at 2 weeks. This method will be important for vaccine and antimicrobial screening work, as it will allow a greater number of candidates to be screened in the same amount of time, which will increase the likelihood that a favorable candidate will be found to be subjected to further testing.

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

PEUBAH KUALITAS AIR YANG MEMPENGARUHI PERTUMBUHAN RUMPUT LAUT (Gracilaria verrucosa) DI TAMBAK TANAH SULFAT MASAM KECAMATAN ANGKONA KABUPATEN LUWU TIMUR PROVINSI SULAWESI SELATAN

2009 ◽  
Vol 4 (1) ◽  
pp. 125
Author(s):  
Akhmad Mustafa ◽  
Rachmansyah Rachmansyah ◽  
Dody Dharmawan Trijuno ◽  
Ruslaini Ruslaini
Keyword(s):  
Water Quality ◽  
Growth Rate ◽  
Ammonium Phosphate ◽  
Gracilaria Verrucosa ◽  
Acid Sulfate Soils ◽  
Acid Sulfate Soil ◽  
Acid Sulfate ◽  
Independent Variables ◽  
Water Quality Variables ◽  
Sulfate Soils

Rumput laut (Gracilaria verrucosa) telah dibudidayakan di tambak tanah sulfat masam dengan kualitas dan kuantitas produksi yang relatif tinggi. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengetahui peubah kualitas air yang mempengaruhi laju pertumbuhan rumput laut di tambak tanah sulfat masam Kecamatan Angkona Kabupaten Luwu Timur Provinsi Sulawesi Selatan. Pemeliharaan rumput laut dilakukan di 30 petak tambak  terpilih selama 6 minggu. Bibit rumput laut dengan bobot 100 g basah ditebar dalam hapa berukuran 1,0 m x 1,0 m x 1,2 m. Peubah tidak bebas yang diamati adalah laju pertumbuhan relatif, sedangkan peubah bebas adalah peubah kualitas air yang meliputi: intensitas cahaya, salinitas, suhu, pH, karbondioksida, nitrat, amonium, fosfat, dan besi. Analisis regresi berganda digunakan untuk menentukan peubah bebas yang dapat digunakan untuk memprediksi peubah tidak bebas. Hasil penelitian menunjukkan bahwa laju pertumbuhan relatif rumput laut di tambak tanah sulfat masam berkisar antara 1,52% dan 3,63%/hari dengan rata-rata 2,88% ± 0,56%/hari. Di antara 9 peubah kualitas air yang diamati ternyata hanya 5 peubah kualitas air yaitu: nitrat, salinitas, amonium, besi, dan fosfat yang mempengaruhi pertumbuhan rumput laut secara nyata. Untuk meningkatkan pertumbuhan rumput laut di tambak tanah sulfat masam Kecamatan Angkona Kabupaten Luwu Timur dapat dilakukan dengan pemberian pupuk yang mengandung nitrogen untuk meningkatkan kandungan amonium dan nitrat serta pemberian pupuk yang mengandung fosfor untuk meningkatkan kandungan fosfat sampai pada nilai tertentu, melakukan remediasi untuk menurunkan kandungan besi serta memelihara rumput laut pada salinitas air yang lebih tinggi, tetapi tidak melebihi 30 ppt.Seaweed (Gracilaria verrucosa) has been cultivated in acid sulfate soil-affected ponds with relatively high quality and quantity of seaweed production. A research has been conducted to study water quality variables that influence the growth of seaweed in acid sulfate soil-affected ponds of Angkona Sub-district East Luwu Regency South Sulawesi Province. Cultivation of seaweed was done for six weeks in 30 selected brackishwater ponds. Seeds of seaweed with weight of 100 g were stocked in hapa sized 1.0 m x 1.0 m x 1.2 m. Dependent variable that was observed was specific growth rate, whereas independent variables were water quality variables including light intensity, salinity, temperature, pH, carbondioxide, nitrate, ammonium, phosphate, and iron. Analyses of multiple regressions were used to determine the independent variables which could be used to predict the dependent variable. Research result indicated that relative growth rate of seaweed in acid sulfate soils-affected brackishwater ponds ranged from 1.52% to 3.63%/day with 2.88% ± 0.56%/day in average. Among nine observed water quality variables, only five variables namely: nitrate, salinity, ammonium, phosphate and iron influence significantly on the growth of seaweed in acid sulfate soils-affected brackishwater ponds. The growth of seaweed in acid sulfate soils-affected brackishwater ponds of Angkona District East Luwu Regency, can be improved by using nitrogen-based fertilizers to increase ammonium and nitrate contents and also fertilizers which contain phosphorus to improve phosphate content to a certain level. Pond remediation to decrease iron content and also rearing seaweed at higher salinity (but less than 30 ppt) can also be alternatives to increase the growth of seaweed.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Unintended consequences of biofuels production?The effects of large-scale crop conversion on water quality and quantity

2010 ◽  
Author(s):  
Heather L. Welch ◽  
Christopher T. Green ◽  
Richard A. Rebich ◽  
Jeannie R.B. Barlow ◽  
Matthew B. Hicks
Keyword(s):  
Water Quality ◽  
Large Scale ◽  
Unintended Consequences ◽  
Water Quality And Quantity

Predictability of fouling-potential of raw water for ultrafiltration membranes

2011 ◽  
Vol 11 (4) ◽  
pp. 481-489
Author(s):  
S. Krause ◽  
A. Obermayer
Keyword(s):  
Water Quality ◽  
Membrane Fouling ◽  
Large Scale ◽  
High Capacity ◽  
Raw Water ◽  
Ultrafiltration Membranes ◽  
Small Water ◽  
Lab Experiments ◽  
Flux Decline ◽  
Public Drinking Water

The public drinking water supply of southern Germany is characterized by a rather decentralized network. Due to the hydrogeological setting in these parts of Germany many of the small water works with an average capacity of 50 m3/h have to treat raw water extracted from karstic or cliffy aquifers. These raw waters tend to be contaminated with particles and pathogens acquired during snowmelt or after strong rainfalls. In the last decade ultrafiltration has become the technology of choice for the removal of the aforementioned contaminants. Flux decline caused by unanticipated membrane fouling is the main limitation for the application of ultrafiltration membranes. This paper describes how membrane fouling phenomena can be predicted by using a statistical approach based on data from large scale filtration systems in combination with field and lab experiments on raw water quality and membrane performance. The data defines water quality and respective fouling phenomena both in technical scale filtration plants and in lab experiments of eleven different raw waters. The method described here is more economically feasible for small water works when compared to typical pilot experiments that are used for high capacity water works.

Large-scale wetland-restoration of the Ronde Venen, The Netherlands

1995 ◽  
Vol 31 (8) ◽  
pp. 197-205 ◽  
Author(s):  
L. L. Bijlmakers ◽  
E. O. A. M. de Swart
Keyword(s):  
Water Quality ◽  
Wetland Restoration ◽  
Pollution Control ◽  
Large Scale ◽  
Major Elements ◽  
Control Mechanisms ◽  
Important Criterion ◽  
Spatial Strategy ◽  
Hydrological Characteristics ◽  
Spatial Composition

For the area of the Ronde Venen a plan for large-scale wetland-restoration and improvement of the water quality was developed. Major elements of the developed spatial strategy are the optimal use of the specific hydrological and ecological characteristics of the area. Based on regional hydrological characteristics within the study area hydrological sub-units were distinguished by connecting discharge and recharge areas. In this way the intake of polluted surface water from outside the area could be minimized, with an optimal use of specific local differences in water quality. Two scenarios were developed and evaluated using hydrological, hydrochemical and ecological models. The scenarios differed in spatial composition and the way the water level was manipulated. In order to optimize water quality, natural and artificial pollution control mechanisms were implemented as well. An important criterion for the evaluation was the extent to which the scenarios succeeded in optimizing conditions for the realization of the ecological goals. The most promising and acceptable scenario has been worked out in further detail.

A modeling approach to simulate impact of climate change in lake water quality: phytoplankton growth rate assessment

1998 ◽  
Vol 37 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Hany Hassan ◽  
Keisuke Hanaki ◽  
Tomonori Matsuo
Keyword(s):  
Climate Change ◽  
Water Quality ◽  
Growth Rate ◽  
Dissolved Oxygen ◽  
Lake Water ◽  
Phytoplankton Growth ◽  
Quality Data ◽  
Model Parameters ◽  
Lake Water Quality ◽  
Phytoplankton Growth Rate

Global climate change induced by increased concentrations of greenhouse gases (especially CO2) is expected to include changes in precipitation, wind speed, incoming solar radiation, and air temperature. These major climate variables directly influence water quality in lakes by altering changes in flow and water temperature balance. High concentration of nutrient enrichment and expected variability of climate can lead to periodic phytoplankton blooms and an alteration of the neutral trophic balance. As a result, dissolved oxygen levels, with low concentrations, can fluctuate widely and algal productivity may reach critical levels. In this work, we will present: 1) recent results of GCMs climate scenarios downscaling project that was held at the University of Derby, UK.; 2) current/future comparative results of a new mathematical lake eutrophication model (LEM) in which output of phytoplankton growth rate and dissolved oxygen will be presented for Suwa lake in Japan as a case study. The model parameters were calibrated for the period of 1973–1983 and validated for the period of 1983–1993. Meterologic, hydrologic, and lake water quality data of 1990 were selected for the assessment analysis. Statistical relationships between seven daily meteorological time series and three airflow indices were used as a means for downscaling daily outputs of Hadley Centre Climate Model (HadCM2SUL) to the station sub-grid scale.

Naphthoquinones and derivatives for chemotherapy: perspectives and limitations of their anti-trypanosomatids activities

2020 ◽  
Vol 26 ◽  
Author(s):  
Luíza Dantas-Pereira ◽  
Edézio F. Cunha-Junior ◽  
Valter V. Andrade-Neto ◽  
John F. Bower ◽  
Guilherme A. M. Jardim ◽  
...  
Keyword(s):  
Large Scale ◽  
Neglected Tropical Diseases ◽  
Folk Medicine ◽  
Leishmania Species ◽  
Parasitic Infections ◽  
Mechanistic Analysis ◽  
Leishmania Spp ◽  
Nanomolar Range

: Chagas disease, Sleeping sickness and Leishmaniasis, caused by trypanosomatids Trypanosoma cruzi, Trypanosoma brucei and Leishmania spp., respectively, are considered neglected tropical diseases, and they especially affect impoverished populations in the developing world. The available chemotherapies are very limited and a search for alternatives is still necessary. In folk medicine, natural naphthoquinones have been employed for the treatment of a great variety of illnesses, including parasitic infections. This review is focused on the anti-trypanosomatid activity and mechanistic analysis of naphthoquinones and derivatives. Among all the series of derivatives tested in vitro, naphthoquinone-derived 1,2,3-triazoles were very active on T. cruzi infective forms in blood bank conditions, as well as in amastigotes of Leishmania spp. naphthoquinones containing a CF3 on a phenyl amine ring inhibited T. brucei proliferation in the nanomolar range, and naphthopterocarpanquinones stood out for their activity on a range of Leishmania species. Some of these compounds showed a promising selectivity index (SI) (30 to 1900), supporting further analysis in animal models. Indeed, high toxicity to the host and inactivation by blood components are crucial obstacles to be overcome to use naphthoquinones and/or their derivatives for chemotherapy. Multidisciplinary initiatives embracing medicinal chemistry, bioinformatics, biochemistry, and molecular and cellular biology need to be encouraged to allow the optimization of these compounds. Large scale automated tests are pivotal for the efficiency of the screening step, and subsequent evaluation of both the mechanism of action in vitro and pharmacokinetics in vivo are essential for the development of a novel, specific and safe derivative, minimizing adverse effects.

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