scholarly journals Ocular Surface Microbiota in Contact Lens Users and Contact-Lens-Associated Bacterial Keratitis

Vision ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 27
Author(s):  
Jasmine Andersson ◽  
Josef K. Vogt ◽  
Marlene D. Dalgaard ◽  
Oluf Pedersen ◽  
Kim Holmgaard ◽  
...  
Keyword(s):  
Contact Lens ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Bacterial Keratitis ◽  
Opportunistic Pathogens ◽  
Contact Lens Wear ◽  
Conjunctival Swab ◽  
Study Population ◽  
Corneal Infiltrates ◽  
Lens Wear

Our objectives were to investigate whether the conjunctival microbiota is altered by contact lens wear and/or bacterial keratitis and to explore the hypothesis that commensals of conjunctival microbiota contribute to bacterial keratitis. Swab samples from both eyes were collected separately from the inferior fornix of the conjunctiva of non-contact-lens users (nparticipants = 28) and contact lens users (nparticipants = 26) and from patients with contact-lens-associated bacterial keratitis (nparticipants = 9). DNA from conjunctival swab samples was analyzed with 16S rRNA gene amplicon sequencing. Pathogens from the corneal infiltrates were identified by cultivation. In total, we identified 19 phyla and 283 genera; the four most abundant genera were Pseudomonas, Enhydrobacter, Staphylococcus, and Cutibacterium. Several pathogens related to bacterial keratitis were identified in the conjunctival microbiota of the whole study population, and the same bacteria were identified by both methods in the conjunctiva and cornea for four patients with contact-lens-associated bacterial keratitis. The overall conjunctival microbiota profile was not altered by contact lens wear or bacterial keratitis; thus, it does not appear to contribute to the development of bacterial keratitis in contact lens users. However, in some individuals, conjunctival microbiota may harbor opportunistic pathogens causing contact-lens-associated bacterial keratitis.

scholarly journals Risk Factors, Clinical Outcomes and Prognostic Factors of Bacterial Keratitis: The Nottingham Infectious Keratitis Study

2021 ◽  
Author(s):  
Darren Shu Jeng Ting ◽  
Jessica Cairns ◽  
Bhavesh Gopal ◽  
Charlotte Ho ◽  
Lazar Krstic ◽  
...  
Keyword(s):  
Risk Factors ◽  
Prognostic Factors ◽  
Contact Lens ◽  
Visual Outcome ◽  
Bacterial Keratitis ◽  
Contact Lens Wear ◽  
Poor Visual Outcome ◽  
Corrected Distance Visual Acuity ◽  
Culture Positive ◽  
Lens Wear

Background/aim: To examine the risk factors, clinical characteristics, outcomes and prognostic factors of bacterial keratitis (BK) in Nottingham, UK. Methods: This was a retrospective study of patients who presented to the Queens Medical Centre, Nottingham, with suspected BK during 2015-2019. Relevant data, including the demographic factors, risk factors, clinical outcomes, and potential prognostic factors, were analysed. Results: A total of 283 patients (n=283 eyes) were included; mean age was 54.4+/-21.0 years and 50.9% were male. Of 283 cases, 128 (45.2%) cases were culture-positive. Relevant risk factors were identified in 96.5% patients, with ocular surface diseases (47.3%), contact lens wear (35.3%) and systemic immunosuppression (18.4%) being the most common factors. Contact lens wear was most commonly associated with P. aeruginosa whereas Staphylococci spp. were most commonly implicated in non-contact lens-related BK cases (p=0.017). At presentation, culture-positive cases were associated with older age, worse presenting corrected-distance-visual-acuity (CDVA), larger epithelial defect and infiltrate, central location and hypopyon (all p<0.01), when compared to culture-negative cases. Hospitalisation was required in 57.2% patients, with a mean length of stay of 8.0 +/- 8.3 days. Surgical intervention was required in 16.3% patients. Significant complications such as threatened/actual corneal perforation (8.8%), loss of perception of light vision (3.9%), and evisceration/enucleation (1.4%) were noted. Poor visual outcome (final corrected-distance-visual-acuity of <0.6 logMAR) and delayed corneal healing (>30 days from initial presentation) were significantly affected by age >50 years, infiltrate size >3mm, and reduced presenting vision (all p<0.05). Conclusion: BK represents a significant ocular morbidity in the UK. Culture positivity is associated with more severe disease at presentation but has no significant influence on the final outcome. Older age, large infiltrate, and poor presenting vision were predictive of poor visual outcome and delayed corneal healing, highlighting the importance of primary prevention and early intervention for BK.

Nonulcerating bacterial keratitis associated with soft and rigid contact lens wear

Ophthalmology ◽  
1998 ◽  
Vol 105 (3) ◽  
pp. 517-521 ◽  
Author(s):  
Stephen D McLeod ◽  
Stephanie L Goei ◽  
Daniel P Taglia ◽  
Timothy T McMahon
Keyword(s):  
Contact Lens ◽  
Bacterial Keratitis ◽  
Contact Lens Wear ◽  
Rigid Contact ◽  
Lens Wear

Corneal Infiltrates Associated With Soft Contact Lens Wear

Cornea ◽  
2004 ◽  
Vol 23 (4) ◽  
pp. 421-422 ◽  
Author(s):  
Jules Baum ◽  
Peter C Donshik
Keyword(s):  
Contact Lens ◽  
Soft Contact ◽  
Contact Lens Wear ◽  
Soft Contact Lens ◽  
Corneal Infiltrates ◽  
Lens Wear

The impact of daily disposable contact lens wear on the conjuctival epitithelium

2015 ◽  
Vol 38 ◽  
pp. e11
Author(s):  
Maria Markoulli ◽  
Moneisha Gokhale ◽  
Jason Chiem ◽  
Flora Lo ◽  
Eric Papas
Keyword(s):  
Contact Lens ◽  
Contact Lens Wear ◽  
The Impact ◽  
Lens Wear
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