scholarly journals Characterization of HIV-1 Near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy

Viruses ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 392 ◽  
Author(s):  
Brunna Alves ◽  
Juliana Siqueira ◽  
Marianne Garrido ◽  
Ornella Botelho ◽  
Isabel Prellwitz ◽  
...  
Keyword(s):  
Viral Load ◽  
Undetectable Viral Load ◽  
Full Length ◽  
Proviral Genome ◽  
First Line ◽  
Hiv 1 ◽  
Haart Therapy

scholarly journals Characterization of HIV-1 near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy

2017 ◽  
Author(s):  
Brunna M. Alves ◽  
Juliana D. Siqueira ◽  
Marianne M. Garrido ◽  
Ornella M. Botelho ◽  
Isabel M. Prellwitz ◽  
...  
Keyword(s):  
Viral Load ◽  
Highly Active Antiretroviral Therapy ◽  
Treatment Success ◽  
Undetectable Viral Load ◽  
Hiv Treatment ◽  
Full Length ◽  
Resistance Mutations ◽  
First Line ◽  
Subtype B ◽  
Hiv 1

Increased access to highly active antiretroviral therapy (HAART) by HIV+ individuals has become a reality worldwide. In Brazil, ART currently reaches over half of the HIV-infected subjects. In the context of a remarkable HIV-1 genetic variability, highly related variants, called quasispecies, are generated. HIV quasispecies generated during infection can influence virus persistence and pathogenicity, representing a challenge to treatment. However, the clinical relevance of minority quasispecies is still uncertain. For this study, we have determined the archived proviral sequences, viral subtype and drug resistance mutations from a cohort of HIV+ patients with undetectable viral load undergoing HAART as first-line therapy using next-generation sequencing for near full-length virus genome (NFLG) assembly. HIV-1 consensus sequences representing NFLG were obtained for eleven patients, while for another twelve varying genome coverage rates were obtained. Phylogenetic analysis showed the predominance of subtype B (83%; 19/23). Considering the minority variants, 18 patients carried archived virus harboring at least one mutation conferring antiretroviral resistance; for six patients, the mutations correlated with the current ARVs used. These data highlight the importance of monitoring HIV minority drug resistant variants and their clinical impact, to guide future regimen switches and improve HIV treatment success.

Expression, purification and characterization of a full-length recombinant HIV-1 Vpu from inclusion bodies

2016 ◽  
Vol 128 ◽  
pp. 109-114 ◽  
Author(s):  
Zikhona Njengele ◽  
Ronel Kleynhans ◽  
Yasien Sayed ◽  
Salerwe Mosebi
Keyword(s):  
Inclusion Bodies ◽  
Full Length ◽  
Purification And Characterization ◽  
Hiv 1

scholarly journals Construction and Characterization of Highly Infectious Full-Length Molecular Clones of a HIV-1 CRF07_BC Isolate from Xinjiang, China

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79177 ◽  
Author(s):  
Zheng Wang ◽  
Kunxue Hong ◽  
Jing Zhang ◽  
Lei Zhang ◽  
Dan Li ◽  
...  
Keyword(s):  
Full Length ◽  
Hiv 1

scholarly journals High Rate of Non-detectable HIV-1 RNA among Antiretroviral Drug Naive HIV Positive Individuals in Nigeria

2013 ◽  
Vol 4 ◽  
pp. VRT.S12677 ◽  
Author(s):  
Georgina N. Odaibo ◽  
Isaac F. Adewole ◽  
David O. Olaleye
Keyword(s):  
Viral Load ◽  
Undetectable Viral Load ◽  
Antiretroviral Drugs ◽  
High Rate ◽  
Baseline Viral Load ◽  
Hiv Positive ◽  
Detectable Viral Load ◽  
Cell Counts ◽  
Drug Naïve ◽  
Hiv 1

Plasma HIV-1 RNA concentration, or viral load, is an indication of the magnitude of virus replication and largely correlates with disease progression in an infected person. It is a very useful guide for initiation of therapy and monitoring of response to antiretroviral drugs. Although the majority of patients who are not on antiretroviral therapy (ART) have a high viral load, a small proportion of ART naive patients are known to maintain low levels or even undetectable viral load levels. In this study, we determined the rate of undetectable HIV-1 RNA among ART naive HIV positive patients who presented for treatment at the University College Hospital (UCH), Ibadan, Nigeria from 2005 to 2011. Baseline viral load and CD4 lymphocyte cell counts of 14,662 HIV positive drug naive individuals were determined using the Roche Amplicor version 1.5 and Partec easy count kit, respectively. The detection limits of the viral load assay are 400 copies/mL and 750,000 copies/mL for lower and upper levels, respectively. A total of 1,399 of the 14,662 (9.5%) HIV-1 positive drug naive individuals had undetectable viral load during the study period. In addition, the rate of non-detectable viral load increased over the years. The mean CD4 counts among HIV-1 infected individuals with detectable viral load (266 cells/μL; range = 1 to 2,699 cells/μL) was lower than in patients with undetectable viral load (557 cells/μL; range = 1 to 3,102 cells/μL). About 10% of HIV-1 infected persons in our study population had undetectable viral load using the Roche Amplicor version 1.5.

scholarly journals Characterization of the Interaction of Full-Length HIV-1 Vif Protein with its Key Regulator CBFβ and CRL5 E3 Ubiquitin Ligase Components

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33495 ◽  
Author(s):  
Xiaohong Zhou ◽  
Sean L. Evans ◽  
Xue Han ◽  
Yayan Liu ◽  
Xiao-Fang Yu
Keyword(s):  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Full Length ◽  
Vif Protein ◽  
Hiv 1

The effect of baseline CD4 cell count and HIV-1 viral load on the efficacy and safety of nevirapine or efavirenz-based first-line HAART

AIDS ◽  
2005 ◽  
Vol 19 (5) ◽  
pp. 463-471 ◽  
Author(s):  
F van Leth ◽  
S Andrews ◽  
B Grinsztejn ◽  
E Wilkins ◽  
MK Lazanas ◽  
...  
Keyword(s):  
Viral Load ◽  
Cell Count ◽  
Efficacy And Safety ◽  
First Line ◽  
Cd4 Cell Count ◽  
Cd4 Cell ◽  
Hiv 1
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