scholarly journals Evaluation and Comparison of the Pathogenicity and Host Immune Responses Induced by a G2b Taiwan Porcine Epidemic Diarrhea Virus (Strain Pintung 52) and Its Highly Cell-Culture Passaged Strain in Conventional 5-Week-Old Pigs

Viruses ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 121 ◽  
Author(s):  
Yen-Chen Chang ◽  
Chi-Fei Kao ◽  
Chia-Yu Chang ◽  
Chian-Ren Jeng ◽  
Pei-Shiue Tsai ◽  
...  
Keyword(s):  
Cell Culture ◽  
Immune Responses ◽  
Virus Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Host Immune Responses ◽  
Porcine Epidemic Diarrhea

scholarly journals Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage

2017 ◽  
Vol 201 ◽  
pp. 62-71 ◽  
Author(s):  
Chun-Ming Lin ◽  
Yixuan Hou ◽  
Douglas G. Marthaler ◽  
Xiang Gao ◽  
Xinsheng Liu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Virus Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Culture Passage ◽  
Porcine Epidemic Diarrhea ◽  
Cell Culture Passage

scholarly journals Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Vaccines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1084
Author(s):  
Fengsai Li ◽  
Xiaona Wang ◽  
Xiaolong Fan ◽  
Ling Sui ◽  
Hailin Zhang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Porcine Epidemic Diarrhea Virus ◽  
Lactobacillus Casei ◽  
Oral Vaccine ◽  
Mucosal Vaccine ◽  
Alanine Racemase ◽  
Main Function ◽  
Neutralizing Epitope ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea (PED), which is caused by the porcine epidemic diarrhea virus (PEDV), has occurred worldwide and poses a serious threat to the pig industry. Intestine is the main function site of PEDV; therefore, it is important to develop an oral mucosal immunity vaccine against this virus infection. Most traditional plasmid delivery vectors use antibiotic genes as a selective marker, easily leading to antibiotic accumulation and gene contamination. In this study, to explore whether the alanine racemase gene (Alr) could be used as a screening marker and develop an efficient oral vaccine against PEDV infection, a recombinant strain was constructed using Lactobacillus casei with Alr deletion (L. casei ΔAlr W56) to deliver the Alr gene and a core-neutralizing epitope (COE) antigen. This recombinant bacterium efficiently induced secretory immunoglobulin A (SIgA)-based mucosal and immunoglobulin G (IgG)-based humoral immune responses via oral vaccination in mice. Compared to the other strains, the recombinant bacteria were able to grow without the addition of D-alanine, revealing that Alr in the plasmid could function normally in defective bacteria. This oral mucosal vaccine would provide a useful strategy to substitute the application of antibiotics in the future and induce efficient immune responses against PEDV infection.

scholarly journals Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 50-61 ◽  
Author(s):  
Baochao Fan ◽  
Zhengyu Yu ◽  
Fengjiao Pang ◽  
Xiangwei Xu ◽  
Baimeng Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Cdna Clone ◽  
Porcine Epidemic Diarrhea Virus ◽  
Full Length ◽  
Diarrhea Virus ◽  
Full Length Cdna ◽  
Porcine Epidemic Diarrhea

scholarly journals Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China

Virus Genes ◽  
2016 ◽  
Vol 52 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Renfeng Li ◽  
Songlin Qiao ◽  
Yanyan Yang ◽  
Junqing Guo ◽  
Sha Xie ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Virus Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Full-Length Genome Sequence of Porcine Epidemic Diarrhea Virus Strain CH/GX/2015/750A

2017 ◽  
Vol 5 (27) ◽  
Author(s):  
Yibin Qin ◽  
Bingxia Lu ◽  
Ying He ◽  
Bin Li ◽  
Qunpeng Duan ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Full Length ◽  
Watery Diarrhea ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Full Length Genome

ABSTRACT We report here the complete genome sequence of porcine epidemic diarrhea virus (PEDV) strain CH/GX/2015/750A (750A), which was isolated from a suckling piglet with watery diarrhea in Guangxi, China. The isolate is genetically close to other recent Chinese variant PEDVs and distinct from the classical PEDVs.

scholarly journals Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs

2014 ◽  
Vol 20 (4) ◽  
pp. 668-671 ◽  
Author(s):  
Kwonil Jung ◽  
Qiuhong Wang ◽  
Kelly A. Scheuer ◽  
Zhongyan Lu ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Gnotobiotic Pigs ◽  
Porcine Epidemic Diarrhea
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