scholarly journals Three-Dimensional Rotating Wall Vessel-Derived Cell Culture Models for Studying Virus-Host Interactions

Viruses ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 304 ◽  
Author(s):  
Jameson Gardner ◽  
Melissa Herbst-Kralovetz
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Host Interactions ◽  
Rotating Wall ◽  
Rotating Wall Vessel ◽  
Culture Models ◽  
Cell Culture Models

Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

2010 ◽  
Vol 8 (11) ◽  
pp. 791-801 ◽  
Author(s):  
Jennifer Barrila ◽  
Andrea L. Radtke ◽  
Aurélie Crabbé ◽  
Shameema F. Sarker ◽  
Melissa M. Herbst-Kralovetz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Host Pathogen Interactions ◽  
Rotating Wall ◽  
Rotating Wall Vessel ◽  
Host Pathogen ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

scholarly journals Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Pirmin H. Lakner ◽  
Michael G. Monaghan ◽  
Yvonne Möller ◽  
Monilola A. Olayioye ◽  
Katja Schenke-Layland
Keyword(s):  
Cell Culture ◽  
Metabolic Activity ◽  
Three Dimensional ◽  
In Vitro Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models

2018 ◽  
Vol 6 ◽  
Author(s):  
Núria Torras ◽  
María García-Díaz ◽  
Vanesa Fernández-Majada ◽  
Elena Martínez
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Epithelial Tissues ◽  
Culture Models ◽  
Cell Culture Models ◽  
Dimensional Cell
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