scholarly journals PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells

Viruses ◽  
2016 ◽  
Vol 8 (6) ◽  
pp. 173 ◽  
Author(s):  
Amr Gamil ◽  
Cheng Xu ◽  
Stephen Mutoloki ◽  
Øystein Evensen
Keyword(s):  
Virus Replication ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infected Cells ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

scholarly journals Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus

2014 ◽  
pp. 3990-4002 ◽  
Author(s):  
Cesar Ortega S ◽  
Sylvia Rodríguez S ◽  
Juan Carlos Espinoza ◽  
Juan Kuznar ◽  
Alex Romero ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pancreatic Necrosis ◽  
Virulent Strain ◽  
Critical Factor ◽  
Infectious Pancreatic Necrosis Virus ◽  
Viral Titer ◽  
Necrosis Virus ◽  
Infected Cells ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

ABSTRACTObjective. To determine whether the level of apoptosis induced by infectious pancreatic necrosis virus (IPNV) is related to the amino acid sequence of the BH2 domain of the VP5 protein and the level of infectivity. Materials and methods. Three IPNV strains were used, the VP2 protein gene was amplified for genotyping and the VP5 sequence was also obtained. The infectivity of the strains was calculated using the viral titer obtained at 12, 24, 36 and 45 hpi in CHSE-214 cells. The percentage of apoptosis in infected cells was visualized by TUNEL assay and immunohistochemistry (caspase 3 detection). Results. The V70/06 and V33/98 strains corresponded to genotype Sp, while V112/06 to VR-299; the amino acid analysis of the V70/06 strain allows its classification as middle virulent strain and V33/98 and V112/06 strains as low virulent ones; infection with the V112/06 strain produced a lower viral titer (p<0.05). The VP5 gene of the 3 strains showed four homologous domains to Bcl-2, however, the BH2 domain was truncated in V70/06 and V33/98 (12 kDa), being complete (15kDa) in V112/06, which also showed the Trp155 residue, equivalent to Trp188 considered as a critical factor for the function of Bcl-2. The average apoptosis was below 12%, showing no differences between strains (p>0.05). Conclusions. The results showed that the differences in the BH2 sequence of the VP5 protein, infectivity and the VP2 sequence are not associated with the modulation of apoptosis.

Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus

1980 ◽  
Vol 26 (2) ◽  
pp. 256-261 ◽  
Author(s):  
Richard D. Macdonald
Keyword(s):  
Infectious Bursal Disease Virus ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Urea Treatment ◽  
Double Stranded Rna ◽  
Bursal Disease ◽  
Infected Cells ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Urea treatment of ethanol-fixed virus-infected cells exposed nucleic acid antigens for immunofluorescence. Three double-stranded (ds) RNA-containing viruses showed bright fluorescence using antibodies against dsRNA. Three single-stranded RNA-containing viruses showed less intense fluorescence with anti-dsRNA. Four out of five cell lines persistently infected with various RNA-containing viruses showed no dsRNA detectable by immunofluorescence.

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