scholarly journals Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts

Viruses ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 5182-5197 ◽  
Author(s):  
Kristen Ciano ◽  
Jason Saredy ◽  
Doria Bowers
Keyword(s):  
Salivary Gland ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Mosquito Salivary Gland ◽  
Attachment Factor

scholarly journals Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses

2017 ◽  
Vol 91 (15) ◽  
Author(s):  
Shin Murakami ◽  
Akiko Takenaka-Uema ◽  
Tomoya Kobayashi ◽  
Kentaro Kato ◽  
Masayuki Shimojima ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Molecular Mechanisms ◽  
Substantial Reduction ◽  
Heparan Sulfate Proteoglycan ◽  
Economic Losses ◽  
Sulfate Proteoglycan ◽  
Content Type ◽  
Cellular Attachment ◽  
Attachment Factor

ABSTRACT Akabane virus (AKAV) and Schmallenberg virus (SBV) are members of the genus Orthobunyavirus, which are transmitted by arthropod vectors with a broad cellular tropism in vitro as well as in vivo. Both AKAV and SBV cause arthrogryposis-hydranencephaly syndrome in ruminants. The main cellular receptor and attachment factor for entry of these orthobunyaviruses are unknown. Here, we found that AKAV and SBV infections were inhibited by the addition of heparin or enzymatic removal of cell surface heparan sulfates. To confirm this finding, we prepared heparan sulfate proteoglycan (HSPG)-knockout (KO) cells by using a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system and measured the quantities of binding of these viruses to cell surfaces. We observed a substantial reduction in AKAV and SBV binding to cells, limiting the infections by these viruses. These data demonstrate that HSPGs are important cellular attachment factors for AKAV and SBV, at least in vitro, to promote virus replication in susceptible cells. IMPORTANCE AKAV and SBV are the etiological agents of arthrogryposis-hydranencephaly syndrome in ruminants, which causes considerable economic losses in the livestock industry. Here, we identified heparan sulfate proteoglycan as a major cellular attachment factor for the entry of AKAV and SBV. Moreover, we found that heparin is a strong inhibitor of AKAV and SBV infections. Revealing the molecular mechanisms of virus-host interactions is critical in order to understand virus biology and develop novel live attenuated vaccines.

Alterations in the basement membrane (heparan sulfate) proteoglycan in diabetic mice

Diabetes ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 185-188 ◽  
Author(s):  
D. H. Rohrbach ◽  
J. R. Hassell ◽  
H. K. Kleinman ◽  
G. R. Martin
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Diabetic Mice

Targeting Mechanism of a Novel Liver-targeting Interferon IFN-CSP Involves Liver Heparan Sulfate Proteoglycan

2016 ◽  
Vol 13 (4) ◽  
pp. 528-533 ◽  
Author(s):  
Xuemei Lu ◽  
Jie Wang ◽  
Xiaobao Jin ◽  
Yanting Huang ◽  
Wenting Zeng ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Liver Targeting

Immunohistochemical Study of Heparan Sulfate Proteoglycan in Adenocarcinomas of the Pancreas

Pancreas ◽  
1994 ◽  
Vol 9 (6) ◽  
pp. 758-763 ◽  
Author(s):  
Zhao-hui Wang ◽  
Tadao Manabe ◽  
Gakuji Ohshio ◽  
Takashi Imamura ◽  
Tsunehiro Yoshimura ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Immunohistochemical Study ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan

scholarly journals Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.

1990 ◽  
Vol 265 (32) ◽  
pp. 19980-19989
Author(s):  
R V Iozzo ◽  
I Kovalszky ◽  
N Hacobian ◽  
P K Schick ◽  
J S Ellingson ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Heparan Sulfate Proteoglycan ◽  
Carcinoma Cells ◽  
Sulfate Proteoglycan ◽  
Fatty Acylation ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma
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