scholarly journals Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

Viruses ◽  
2013 ◽  
Vol 5 (10) ◽  
pp. 2601-2613 ◽  
Author(s):  
Jianfei Chen ◽  
Xiaozhen Liu ◽  
Da Shi ◽  
Hongyan Shi ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Molecular Diversity ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Huinan Wang ◽  
Libo Zhang ◽  
Yuanbin Shang ◽  
Rongrong Tan ◽  
Mingxiang Ji ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Intestinal Epithelial ◽  
Orf3 Gene ◽  
Highly Pathogenic ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Attenuated Vaccine ◽  
New Variant ◽  
Porcine Epidemic Diarrhea ◽  
Natural Recombination

Abstract Outbreaks of a new variant of porcine epidemic diarrhea virus (PEDV) at the end of 2010 have raised interest in the mutation and recombination of PEDV. A PEDV strain (CN/Liaoning25/2018) isolated from a clinical outbreak of piglet diarrhea contained a 49-bp deletion in the ORF3 gene. This deletion is considered a genetic characteristic of low pathogenic attenuated vaccine strains. However, CN/Liaoning25/2018 was highly pathogenic. Complete genome sequencing, identity analysis, phylogenetic tree construction, and recombination analysis showed that this virus was a recombinant strain containing the Spike (S) gene from the highly pathogenic CN/GDZQ/2014 strain and the remaining genomic regions from the low pathogenic vaccine isolate SQ2014. Histopathology and immunohistochemistry results confirmed that this strain was highly pathogenic and indicated that intestinal epithelial cell vacuolation was positively correlated with the intensity and density of PEDV antigens. A new natural recombination model for PEDV was identified. Our results suggest that new highly pathogenic recombinant strains in the field may be generated by recombination between low pathogenic attenuated live PEDV vaccines and pathogenic circulating PEDV strains. Our findings also highlight that the 49-bp deletion of the ORF3 gene in low pathogenic attenuated vaccine strains will no longer be a reliable standard to differentiate the classical vaccine attenuated from the field strains.

scholarly journals Molecular characterization of the spike gene of the porcine epidemic diarrhea virus in Mexico, 2013–2016

Virus Genes ◽  
2017 ◽  
Vol 54 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Rocío Lara-Romero ◽  
Luis Gómez-Núñez ◽  
José Luis Cerriteño-Sánchez ◽  
Laura Márquez-Valdelamar ◽  
Susana Mendoza-Elvira ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Epidemiology and phylogeny of spike gene of porcine epidemic diarrhea virus from Yunnan, China

2018 ◽  
Vol 249 ◽  
pp. 45-51 ◽  
Author(s):  
Qingwei Zuo ◽  
Ru Zhao ◽  
Jia Liu ◽  
Qian Zhao ◽  
Lingyun Zhu ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Deletion in the S1 Region of Porcine Epidemic Diarrhea Virus Reduces the Virulence and Influences the Virus-Neutralizing Activity of the Antibody Induced

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1378
Author(s):  
Kuo-Jung Tsai ◽  
Ming-Chung Deng ◽  
Fun-In Wang ◽  
Shu-Hui Tsai ◽  
Chieh Chang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Porcine Epidemic Diarrhea Virus ◽  
Clinical Signs ◽  
Large Deletion ◽  
High Rate ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Severe Diarrhea ◽  
Insertion And Deletion ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and a high rate of mortality in suckling pigs. The epidemic of PEDV that occurred after 2013 was caused by non-insertion and deletion of S gene (S-INDEL) PEDV strains. During this epidemic, a variant of the non-S-INDEL PEDV strain with a large deletion of 205 amino acids on the spike gene (5-17-V) was also found to co-exist with a non-S-INDEL PEDV without deletion (5-17-O). Herein, we describe the differences in the complete genome, distribution, virulence, and antigenicity between strain 5-17-O and variant strain 5-17-V. The deletion of 205 amino acids was primarily located in the S1O domain and was associated with milder clinical signs and lower mortality in suckling pigs than those of the 5-17-O strain. The 5-17-V strain-induced antibody did not completely cross-neutralize the 5-17-O strain. In conclusion, the deletion in the S1 region reduces the virulence of PEDV and influences the virus-neutralizing activities of the antibody it induces.

scholarly journals Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo

Virus Genes ◽  
2011 ◽  
Vol 43 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Tetsuo Sato ◽  
Natsumi Takeyama ◽  
Atsushi Katsumata ◽  
Kotaro Tuchiya ◽  
Toshiaki Kodama ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Growth Adaptation
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