scholarly journals Viral and Host Factors Required for Avian H5N1 Influenza A Virus Replication in Mammalian Cells

Viruses ◽  
2013 ◽  
Vol 5 (6) ◽  
pp. 1431-1446 ◽  
Author(s):  
Hong Zhang ◽  
Benjamin Hale ◽  
Ke Xu ◽  
Bing Sun
Keyword(s):  
Influenza A Virus ◽  
Virus Replication ◽  
Mammalian Cells ◽  
Influenza A ◽  
Host Factors ◽  
H5n1 Influenza

scholarly journals An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaofeng Huang ◽  
Min Zheng ◽  
Pui Wang ◽  
Bobo Wing-Yee Mok ◽  
Siwen Liu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Virus Replication ◽  
Mammalian Cells ◽  
Influenza A ◽  
Exonic Splicing Enhancer ◽  
Splicing Enhancer

scholarly journals Role of TGF-β-activated kinase 1 (TAK1) activation in H5N1 influenza A virus-induced c-Jun terminal kinase activation and virus replication

Virology ◽  
2019 ◽  
Vol 537 ◽  
pp. 263-271 ◽  
Author(s):  
Tianyu Sheng ◽  
Yuling Sun ◽  
Jing Sun ◽  
Richard A. Prinz ◽  
Daxin Peng ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
Kinase Activation ◽  
H5n1 Influenza

scholarly journals The Nucleolar Protein LYAR Facilitates Ribonucleoprotein Assembly of Influenza A Virus

2018 ◽  
Vol 92 (23) ◽  
Author(s):  
Cha Yang ◽  
Xiaokun Liu ◽  
Qingxia Gao ◽  
Tailang Cheng ◽  
Rong Xiao ◽  
...  
Keyword(s):  
Cell Growth ◽  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
Rna Synthesis ◽  
Nucleolar Protein ◽  
Host Factors ◽  
Viral Rna ◽  
A Cell ◽  
Transcription And Replication

ABSTRACTInfluenza A viral ribonucleoprotein (vRNP) is responsible for transcription and replication of the viral genome in infected cells and depends on host factors for its functions. Identification of the host factors interacting with vRNP not only improves understanding of virus-host interactions but also provides insights into novel mechanisms of viral pathogenicity and the development of new antiviral strategies. Here, we have identified 80 host factors that copurified with vRNP using affinity purification followed by mass spectrometry. LYAR, a cell growth-regulating nucleolar protein, has been shown to be important for influenza A virus replication. During influenza A virus infection, LYAR expression is increased and partly translocates from the nucleolus to the nucleoplasm and cytoplasm. Furthermore, LYAR interacts with RNP subunits, resulting in enhancing viral RNP assembly, thereby facilitating viral RNA synthesis. Taken together, our studies identify a novel vRNP binding host partner important for influenza A virus replication and further reveal the mechanism of LYAR regulating influenza A viral RNA synthesis by facilitating viral RNP assembly.IMPORTANCEInfluenza A virus (IAV) must utilize the host cell machinery to replicate, but many of the mechanisms of IAV-host interaction remain poorly understood. Improved understanding of interactions between host factors and vRNP not only increases our basic knowledge of the molecular mechanisms of virus replication and pathogenicity but also provides insights into possible novel antiviral targets that are necessary due to the widespread emergence of drug-resistant IAV strains. Here, we have identified LYAR, a cell growth-regulating nucleolar protein, which interacts with viral RNP components and is important for efficient replication of IAVs and whose role in the IAV life cycle has never been reported. In addition, we further reveal the role of LYAR in viral RNA synthesis. Our results extend and improve current knowledge on the mechanisms of IAV transcription and replication.

scholarly journals Effect on Virulence and Pathogenicity of H5N1 Influenza A Virus through Truncations of NS1 eIF4GI Binding Domain

2010 ◽  
Vol 202 (9) ◽  
pp. 1338-1346 ◽  
Author(s):  
Hongbo Zhou ◽  
Jiping Zhu ◽  
Jiagang Tu ◽  
Wei Zou ◽  
Yong Hu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Binding Domain ◽  
H5n1 Influenza

scholarly journals Dicer is involved in protection against influenza A virus infection

2007 ◽  
Vol 88 (10) ◽  
pp. 2627-2635 ◽  
Author(s):  
Alexey A. Matskevich ◽  
Karin Moelling
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Mammalian Cells ◽  
Influenza A ◽  
Virus Production ◽  
Vero Cells ◽  
Antiviral Response ◽  
Protein Levels ◽  
Infected Cells ◽  
Influenza A Virus Infection

In mammals the interferon (IFN) system is a central innate antiviral defence mechanism, while the involvement of RNA interference (RNAi) in antiviral response against RNA viruses is uncertain. Here, we tested whether RNAi is involved in the antiviral response in mammalian cells. To investigate the role of RNAi in influenza A virus-infected cells in the absence of IFN, we used Vero cells that lack IFN-α and IFN-β genes. Our results demonstrate that knockdown of a key RNAi component, Dicer, led to a modest increase of virus production and accelerated apoptosis of influenza A virus-infected cells. These effects were much weaker in the presence of IFN. The results also show that in both Vero cells and the IFN-producing alveolar epithelial A549 cell line influenza A virus targets Dicer at mRNA and protein levels. Thus, RNAi is involved in antiviral response, and Dicer is important for protection against influenza A virus infection.

scholarly journals Characterization of a Human H5N1 Influenza A Virus Isolated in 2003

2005 ◽  
Vol 79 (15) ◽  
pp. 9926-9932 ◽  
Author(s):  
Kyoko Shinya ◽  
Masato Hatta ◽  
Shinya Yamada ◽  
Ayato Takada ◽  
Shinji Watanabe ◽  
...  
Keyword(s):  
Hong Kong ◽  
Avian Influenza ◽  
Influenza A Virus ◽  
Influenza A ◽  
Mainland China ◽  
Virus Infections ◽  
Influenza A Viruses ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Influenza ◽  
Avian Influenza A Virus

ABSTRACT In 2003, H5N1 avian influenza virus infections were diagnosed in two Hong Kong residents who had visited the Fujian province in mainland China, affording us the opportunity to characterize one of the viral isolates, A/Hong Kong/213/03 (HK213; H5N1). In contrast to H5N1 viruses isolated from humans during the 1997 outbreak in Hong Kong, HK213 retained several features of aquatic bird viruses, including the lack of a deletion in the neuraminidase stalk and the absence of additional oligosaccharide chains at the globular head of the hemagglutinin molecule. It demonstrated weak pathogenicity in mice and ferrets but caused lethal infection in chickens. The original isolate failed to produce disease in ducks but became more pathogenic after five passages. Taken together, these findings portray the HK213 isolate as an aquatic avian influenza A virus without the molecular changes associated with the replication of H5N1 avian viruses in land-based poultry such as chickens. This case challenges the view that adaptation to land-based poultry is a prerequisite for the replication of aquatic avian influenza A viruses in humans.

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