scholarly journals On-Demand Patient-Specific Phenotype-to-Genotype Ebola Virus Characterization

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2010
Author(s):  
Brett F. Beitzel ◽  
Sheli R. Radoshitzky ◽  
Nicholas Di Paola ◽  
Jennifer M. Brannan ◽  
David Kimmel ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Viral Entry ◽  
Reverse Genetics ◽  
Ebola Virus ◽  
De Novo ◽  
Geographic Origin ◽  
Patient Specific ◽  
Clinical Samples ◽  
Sequence Information ◽  
Genome Sequence Information

Biosafety, biosecurity, logistical, political, and technical considerations can delay or prevent the wide dissemination of source material containing viable virus from the geographic origin of an outbreak to laboratories involved in developing medical countermeasures (MCMs). However, once virus genome sequence information is available from clinical samples, reverse-genetics systems can be used to generate virus stocks de novo to initiate MCM development. In this study, we developed a reverse-genetics system for natural isolates of Ebola virus (EBOV) variants Makona, Tumba, and Ituri, which have been challenging to obtain. These systems were generated starting solely with in silico genome sequence information and have been used successfully to produce recombinant stocks of each of the viruses for use in MCM testing. The antiviral activity of MCMs targeting viral entry varied depending on the recombinant virus isolate used. Collectively, selecting and synthetically engineering emerging EBOV variants and demonstrating their efficacy against available MCMs will be crucial for answering pressing public health and biosecurity concerns during Ebola disease (EBOD) outbreaks.

scholarly journals Draft Genome Report of Bacillus altitudinis SORB11, Isolated from the Indian Sector of the Southern Ocean

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Urmi Halder ◽  
Aparna Banerjee ◽  
Vasvi Chaudhry ◽  
Rajeev K. Varshney ◽  
Shrikant Mantri ◽  
...  
Keyword(s):  
Southern Ocean ◽  
Uv Radiation ◽  
Genome Sequence ◽  
Draft Genome ◽  
Resistance Mechanism ◽  
Sequence Information ◽  
Content Type ◽  
Bacillus Altitudinis ◽  
Genome Sequence Information ◽  
Indian Sector

ABSTRACT Here, we present the draft genome sequence of Bacillus altitudinis SORB11, which is tolerant to UV radiation. The strain was isolated from the Indian sector of the Southern Ocean at a depth of 3.8 km. The genome sequence information reported here for B. altitudinis SORB11 gives the basis of its UV resistance mechanism and provides data for further comparative studies with other bacteria resistant to UV radiation.

scholarly journals Complete Genome Sequences of Two Mutacin-Producing Streptococcus mutans Strains, T8 and UA140

2020 ◽  
Vol 9 (24) ◽  
Author(s):  
Indranil Biswas
Keyword(s):  
Antimicrobial Peptides ◽  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome ◽  
Clinical Isolates ◽  
Sequence Information ◽  
Genome Sequences ◽  
Content Type ◽  
Genome Sequence Information

ABSTRACT Streptococcus mutans is known to produce various antimicrobial peptides called mutacins. Two clinical isolates, T8 and UA140, are well characterized regarding their mutacin production, but genome sequence information was previously unavailable. Complete genome sequences of these two mutacin-producing strains are reported here.

scholarly journals Reverse Genetics for Functional Genomics of Phytopathogenic Fungi and Oomycetes

2009 ◽  
Vol 2009 ◽  
pp. 1-11 ◽  
Author(s):  
Vijai Bhadauria ◽  
Sabine Banniza ◽  
Yangdou Wei ◽  
You-Liang Peng
Keyword(s):  
Functional Genomics ◽  
Gene Disruption ◽  
Insertional Mutagenesis ◽  
Reverse Genetics ◽  
Phytopathogenic Fungi ◽  
Sequence Information ◽  
Biological Functions ◽  
Targeted Gene Disruption ◽  
Local Lesions ◽  
Genome Sequence Information

Sequencing of over 40 fungal and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is now to translate this plethora of genome sequence information into biological functions. Reverse genetics has emerged as a seminal tool for functional genomics investigations. Techniques utilized for reverse genetics like targeted gene disruption/replacement, gene silencing, insertional mutagenesis, and targeting induced local lesions in genomes will contribute greatly to the understanding of gene function of fungal and oomycete pathogens. This paper provides an overview on high-throughput reverse genetics approaches to decode fungal/oomycete genomes.

scholarly journals Draft Genome Sequences of Two Novel Sub-Antarctic Williamsia Species

2017 ◽  
Vol 5 (41) ◽  
Author(s):  
Akhikun Nahar ◽  
Anthony L. Baker ◽  
Michael A. Charleston ◽  
John P. Bowman ◽  
Margaret L. Britz
Keyword(s):  
Genome Sequence ◽  
Metabolic Pathways ◽  
Draft Genome ◽  
Illumina Miseq ◽  
Shotgun Sequencing ◽  
Sequence Information ◽  
Sequencing Technology ◽  
Genome Sequences ◽  
Cold Tolerant ◽  
Genome Sequence Information

ABSTRACT Illumina MiSeq shotgun sequencing technology was used to sequence the genomes of two novel sub-Antarctic Williamsia species, designated strains 1135 and 1138. The estimated genome sizes for strains 1135 and 1138 are 5.99 Mb and 6.08 Mb, respectively. This genome sequence information will aid in understanding the lipid metabolic pathways of cold-tolerant Williamsia species.

scholarly journals Draft Genome Sequence of Microbacterium sp. Strain HM58-2, Which Hydrolyzes Acylhydrazides

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Tomonori Akiyama ◽  
Taichiro Ishige ◽  
Yu Kanesaki ◽  
Shinsaku Ito ◽  
Ken-Ichi Oinuma ◽  
...  
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Sequence Information ◽  
Genome Sequence Information ◽  
Ecological Applications

We report the draft genome sequence of Microbacterium sp. strain HM58-2, which produces hydrazidase, an enzyme hydrolyzing acylhydrazides. The estimated genome size is 3.9 Mb. Genome sequence information of this strain will help to identify an assimilating mechanism of nonnatural compounds in this strain and to develop ecological applications.

scholarly journals Complete Genome Sequence of Burkholderia stabilis FERMP-21014

2017 ◽  
Vol 5 (29) ◽  
Author(s):  
Kenji Konishi ◽  
Toshitaka Kumagai ◽  
Shin-ichi Sakasegawa ◽  
Tomohiro Tamura
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Commercial Production ◽  
Cholesterol Esterase ◽  
Sequence Information ◽  
Content Type ◽  
Genome Sequence Information

ABSTRACT Cholesterol esterase (EC 3.1.1.13) was identified in a bacterium, Burkholderia stabilis strain FERMP-21014. Here, we report the complete genome sequence of B. stabilis FERMP-21014, which has been used in the commercial production of cholesterol esterase. The genome sequence information may be useful for improving production levels of cholesterol esterase.

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