scholarly journals Phage Therapy Experience at the Eliava Phage Therapy Center: Three Cases of Bacterial Persistence

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1901
Author(s):  
Elisabed Zaldastanishvili ◽  
Lika Leshkasheli ◽  
Mariam Dadiani ◽  
Lia Nadareishvili ◽  
Lia Askilashvili ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Phage Therapy ◽  
Adaptation Strategy ◽  
Bacterial Strains ◽  
Patient Case ◽  
Before And After ◽  
Therapy Center ◽  
Pfge Profiles

In this retrospective descriptive study we focus on cases of three patients who underwent phage therapy procedures at Eliava Phage Therapy Center (EPTC) in Tbilisi, Georgia. Patients with chronic infectious diseases related to Pseudomonas aeruginosa (two patients, lower respiratory tract infection (LRTI)) and Klebsiella pneumoniae (one patient, urinary tract infection (UTI)) are among those very few EPTC patients whose pathogens persisted through phage therapy. By looking at bacterial strains and personalized phages used against them we tried to point towards possible adaptation strategies that are employed by these pathogens. Genome restriction-based Pulsed Field Gel Electrophoresis (PFGE) profiling of strains isolated before and after phage therapy hints towards two strategies of adaptation. In one patient case (Pseudomonas aeruginosa related lung infection) bacterial strains before and after phage therapy were indistinguishable according to their PFGE profiles, but differed in their phage susceptibility properties. On the other hand, in two other patient cases (Pseudomonas aeruginosa related LRTI and Klebsiella pneumoniae related UTI) bacterial adaptation strategy seemed to have resulted in diversification of infecting strains of the same species. With this work we want to attract more attention to phage resistance in general as well as to its role in phage therapy.

scholarly journals Phage Therapy Against Biofilm of Multidrug-Resistant Klebsiella Pneumoniae Isolated from Zakho Hospital Samples

2019 ◽  
Vol 9 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Yousif Abdullah AlBany ◽  
Mohammad Ismail Al-Berfkani ◽  
Mahde Saleh Assaf
Keyword(s):  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Phage Therapy ◽  
Multidrug Resistant ◽  
Antimicrobial Treatment ◽  
Bacterial Biofilm ◽  
Hospital Environment ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Extracellular Materials

Klebsiella pneumoniae causes infection in human, especially in immunocompromised patients. About 80% of nosocomial infection caused by K. pneumoniae is due to multidrug-resistant strain. The emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial therapies, which led to studying the ability of viruses that infect the bacteria (known as bacteriophage) to treat infection with K. pneumoniae. Bacterial biofilm which are crucial in the pathogenesis of much clinically important infection and are difficult to eradicate because they exist resistant to many antimicrobial treatment. Biofilm formation by K. pneumoniae is responsible for the catheter associated infection such as urinary tract infection and respiratory tract infection due to the colonization of the polymeric surface by forming multilayered cell cluster embedded in extracellular materials. In this study K. pneumoniae isolated from the hospital environment and characterized it and form the biofilm of that organism by microplate quantitative assay. Similarly bacteriophage specific for K. pneumoniae isolated from river water. The aim of work is the use of bacteriophage as a possible alternative for the treatment of bacterial infection of K. pneumoniae. We showed that biofilm is reduced by isolated phages by the comparative account of colony-forming unit versus plaque-forming unit. The result of this study, therefore, suggests that the timing of starting the phage therapy after initiation of infection significantly contributes toward the success of the treatment.

scholarly journals Prevalence of Urinary Tract Infection among Adult Females in Omu-Aran South-West Nigeria

2020 ◽  
pp. 16-24
Author(s):  
Ndako James A. ◽  
Akinyomade Owolabi ◽  
Oludolapo Olatinsu ◽  
Adedapo O. Omolade ◽  
Ilochi Ifeanyi
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Candida Albicans ◽  
Urinary Tract ◽  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Pregnant Women ◽  
Bacterial Growth ◽  
Proteus Species

Background: Urinary tract infection is caused by the presence and replication of microorganisms in the urinary tract. Urinary tract infection is a major health problem among females especially among pregnant women and this is caused by the physiological makeup of the female urinary tract. Objective: This study was designed to assess the epidemiology of UTIs present amidst pregnant women and females residing in the Omu-Aran community. Materials and Methods: A total of 100 individuals were involved of which 50 were pregnant women while the remaining 50 were non-pregnant females. Semi-structured questionnaires were distributed to all participants to obtain their data. Samples of urine (early morning midstream) were collected in universal bottles from participants. The urine specimens were cultured on four different agar plates which were; cystein lactose electrolyte deficient agar, blood agar, MacConkey agar and nutrient agar for significant bacteria growth. Microscopic and macroscopic examination was also carried out on the samples for possible detection of infections. Results: The result of the culture showed a significant bacterial growth of 77% and 23% of the samples collected showed no significant bacterial growth. Bacteria such as Escherichia coli, Klebsiella pneumoniae, Staphylococcus spp., Proteus species, Pseudomonas aeruginosa and Candida albicans were isolated. With Staphylococcus aureus predominantly present and observed as the causative factor for (45.4%) occurrence of UTIs, closely followed by Staphylococcus saprophyticus (13.0%), Escherichia coli (13.0%), Klebsiella pneumoniae (9.1%) Proteus species (9.1%), Staphylococcus epidermidis (7.8%) and Pseudomonas aeruginosa (1.3%). However the only fungi isolated was Candida albicans showing (1.3%) prevalence, Candida is the most common cause of fungal infections in humans. Conclusion: Results obtained in this work showed a high prevalence of Urinary tract infection at our study location. A prompt enlightenment campaign and need for periodic check among females is highly advocated to reduce or out rightly eliminate the spread of common uropathogens in circulation.

scholarly journals Novel Alkali-Activated Materials with Photocatalytic and Bactericidal Properties Based on Ceramic Tile Waste

Coatings ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 35
Author(s):  
Ashley Bonilla ◽  
Mónica A. Villaquirán-Caicedo ◽  
Ruby Mejía de Gutiérrez
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Photocatalytic Activity ◽  
Klebsiella Pneumoniae ◽  
Room Temperature ◽  
Bacterial Strains ◽  
Cement Pastes ◽  
Molar Ratios ◽  
Bactericidal Properties ◽  
Mechanical And Physical Properties ◽  
Alkali Activated

Ceramics tile wastes (CWs) were mechanically conditioned for the preparation of alkali-activated hybrid-cements from CW (90 wt.%) and Portland cement (10 wt.%) mixtures using sodium silicate (SS) + NaOH as alkaline activators. Molar ratios of SiO2/Al2O3 (6.3 to 7.7) and Na2O/SiO2 (0.07 to 0.16) were used. The cements were prepared at room temperature (25 °C) and characterized by mechanical and physical properties and microstructure. The optimized cement was used for the preparation of novel photoactivated composite materials by incorporating 5 and 10 wt.% TiO2 (Ti) and ZnO (Z) nanoparticles, and its self-cleaning and bactericidal properties were evaluated by means of the degradation of rhodamine-B (Rh-B) and the growth inhibition of Klebsiella pneumoniae and Pseudomonas aeruginosa bacteria. The results of this study showed that the 100SS-5Z and 50SS:50G-10Ti cements have an effective photocatalytic activity for Rh-B degradation of 98.4% and 76.4%, respectively, after 24 h. Additionally, the 100SS-5Z and 50SS:50G-10Ti cement pastes and their respective mortars were effective in inhibiting the growth of Pseudomonas Aeruginosa and Klebsiella pneumoniae bacterial strains, evidenced by the formation of bacterial inhibition halos around the sample discs. Finally, these results are novel, and open the possibility of using constructions and demolition tile waste in high proportions for the elaboration of new rendering mortar with innovative properties.

scholarly journals Comparison of Petroleum Hydrocarbons Degradation by Klebsiella pneumoniae and Pseudomonas aeruginosa

2018 ◽  
Vol 8 (12) ◽  
pp. 2551 ◽  
Author(s):  
Zhaoyang You ◽  
Haiyang Xu ◽  
Shujuan Zhang ◽  
Hyunook Kim ◽  
Pen-Chi Chiang ◽  
...  
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Petroleum Hydrocarbons ◽  
Abiotic Factors ◽  
Lower Surface ◽  
Temporal Variations ◽  
Bacterial Strains ◽  
Relative Growth ◽  
Diesel Degradation

: The aim of this work was to develop bacterial communities to effectively degrade petroleum hydrocarbons (PHs). We investigated the biotic and abiotic contributors to differences in PHs degradation efficacy between two bacterial strains, Klebsiella pneumoniae (Kp) and Pseudomonas aeruginosa (Pa), screened out from the activated sludge of a petroleum refinery. We characterized the temporal variations in degradation efficacy for diesel and its five major constituents as a sole carbon source and identified more constituents they degraded. The growth characteristics, surface tension, hydrophobicity and emulsifiability of these two strains were measured. We further estimated the relationships between their degradation efficacy and all the biotic and abiotic factors. Results showed that the Pa strain had higher diesel degradation efficacy (58% on Day 14) and utilized more diesel constituents (86%) compared to Kp. Additionally, the growth of the Pa strain in diesel medium was faster than that of the Kp strain. The Pa strain had a lower surface tension and higher hydrophobicity and emulsifiability than Kp, while the surfactant produced by Pa was identified as rhamnolipids. Degradation of PHs was positively related to bacterial growth, hydrophobicity and emulsification but negatively related to surface tension. Overall, differences in degrading capacity for diesel constituents, relative growth rate, and biosurfactant production contributed to the variation in the PHs degradation efficacy of these two bacterial strains.

scholarly journals Novel Lytic Phages Protect Cells and Mice against Pseudomonas aeruginosa Infection

2021 ◽  
Author(s):  
Feng Chen ◽  
Xingjun Cheng ◽  
Jianbo Li ◽  
Xiefang Yuan ◽  
Xiuhua Huang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mouse Models ◽  
Bacterial Infections ◽  
Phage Therapy ◽  
Inflammatory Responses ◽  
Multi Drug Resistance ◽  
Bacterial Strains ◽  
Genetic Traits

With the fast emergence of serious antibiotic resistance and the lagged discovery of novel antibacterial drugs, phage therapy for pathogenic bacterial infections has acquired great attention in the clinics. However, development of therapeutic phages also faces tough challenges, such as laborious screening and time to generate effective phage drugs since each phage may only lyse a narrow scope of bacterial strains. Identifying highly effective phages with broad host ranges is crucial for improving phage therapy. Here, we isolated and characterized several lytic phages from various environments specific for Pseudomonas aeruginosa by testing their growth, invasion, host ranges, and potential for killing targeted bacteria. Importantly, we identified several therapeutic phages (HX1, PPY9, and TH15) with broad host ranges to lyse laboratory strains and clinical isolates of P. aeruginosa with multi-drug resistance (MDR) both in vitro and in mouse models. In addition, we analyzed critical genetic traits related to the high-level broad host coverages by genome sequencing and subsequent computational analysis against known phages. Collectively, our findings establish that these novel phages may have potential for further development as therapeutic options for patients who fail to respond to conventional treatments. IMPORTANCE Novel lytic phages isolated from various environmental settings were systematically characterized for their critical genetic traits, morphology structures, host ranges against laboratory strains and clinical multi-drug resistant (MDR) Pseudomonas aeruginosa, and antibacterial capacity both in vitro and in mouse models. First, we characterized the genetic traits and compared with other existing phages. Furthermore, we utilized acute pneumonia induced by laboratorial strain PAO1, and W19, an MDR clinical isolate and chronic pneumonia by agar beads laden with FDR1, a mucoid phenotype strain isolated from the sputum of a cystic fibrosis (CF) patient. Consequently, we found that these phages not only suppress bacteria in vitro but also significantly reduce the infection symptom and disease progression in vivo, including lowered bug burdens, inflammatory responses and lung injury in mice, suggesting that they may be further developed as therapeutic agents against MDR P. aeruginosa.

Emergence of Pseudomonas aeruginosa with KPC-type carbapenemase in a teaching hospital: an 8-year study

2013 ◽  
Vol 62 (10) ◽  
pp. 1565-1570 ◽  
Author(s):  
Dolores García Ramírez ◽  
Federico Nicola ◽  
Soledad Zarate ◽  
Silvia Relloso ◽  
Jorgelina Smayevsky ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Resistance Mechanism ◽  
Control Measures ◽  
Hospital Environment ◽  
Adequate Control ◽  
Early Implementation ◽  
Before And After

An outbreak of Klebsiella pneumoniae carbapenamase (KPC)-producing K. pneumoniae occurred at our institution. Multiresistant Pseudomonas aeruginosa could have acquired this transmissible resistance mechanism, going unnoticed because its phenotypic detection in this species is difficult. We compared P. aeruginosa isolates obtained before and after the KPC-producing K. pneumoniae outbreak. No bla KPC genes were detected in the isolates obtained before the outbreak, whereas 33/76 (43 %) of the isolates obtained after the outbreak harboured the bla KPC gene. P. aeruginosa may thus become a reservoir of this transmissible resistance mechanism. It is very important to understand the epidemiology of these multiresistant isolates, in order to achieve early implementation of adequate control measures to contain and reduce their dissemination in the hospital environment.

scholarly journals The Analysis Of Antibiotic Consumption And Bacterial Resistance As An Indicator Of Their Proper Use At The Urology Department In The Health Centre “Studenica” Kraljevo

2015 ◽  
Vol 16 (2) ◽  
pp. 135-141
Author(s):  
Andriana Bukonjić ◽  
Srđan Stefanović
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Bacterial Resistance ◽  
Health Centre ◽  
Antibiotic Consumption ◽  
Resistance Rate ◽  
Gram Negative Bacteria ◽  
Financial Assets ◽  
Bacterial Strains ◽  
Gram Negative

Abstract The objective of the study was to analyze antibiotic consumption and determine bacterial resistance rates as an indicator of the rational utilization of this drug group at the urology department in the Health Centre “Studenica” Kraljevo. Over a two-year period, the average antibiotic consumption was 104.55 DDD/100BD. Of the total financial assets used for medical treatment, the antibiotic group JO1 had a share of 49.52% in 2011 and 47.53% in 2012. Antibacterial drugs from a group of β-lactamic antibiotics were consumed most commonly, at 54.02% (2011) and 43.44% (2012). First-generation cephalosporins, quinolones and aminoglycosides were the most frequently used drug groups, while cephalexin was the antibiotic with the highest individual consumption. E. coli was the most frequently isolated bacterium in 2011, while in 2012, Klebsiella pneumoniae was the most frequently isolated bacterium. The total bacterial resistance both in 2011 and 2012 was above 50%. Gram-negative bacteria showed a higher resistance rate (2011, 59.3%; 2012, 58.9%) than Gram-positive bacteria (2011, 44.4%; 2012, 40.6%). Klebsiella pneumoniae was the bacterium with the highest resistance (75.3%) in 2011, while in 2012, there was a resistance increase in Pseudomonas aeruginosa (71.4%), especially to carbapenems. A correlation was determined between the consumption of individual antibiotics and bacterial strain resistance in 2011 (r=0.433, p=0.044) and in 2012 (r=0.478, p=0.024). The high resistance rate shown in the bacterial strains, which was correlated with antibiotic consumption, as well as the great financial assets used for this group of drugs suggest the necessity for the rationalization of their utilization. Empirical therapy with Gram-negative bacteria should be based on carbapenems utilization, except with Pseudomonas aeruginosa, where piperacillin/tazobactam should be used.

scholarly journals Therapeutic potential of bacteriophage in treating Klebsiella pneumoniae B5055-mediated lobar pneumonia in mice

2008 ◽  
Vol 57 (12) ◽  
pp. 1508-1513 ◽  
Author(s):  
Sanjay Chhibber ◽  
Sandeep Kaur ◽  
Seema Kumari
Keyword(s):  
Klebsiella Pneumoniae ◽  
Therapeutic Agent ◽  
Phage Therapy ◽  
Therapeutic Potential ◽  
Respiratory Infections ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Lobar Pneumonia ◽  
Resistant Strains

Klebsiella pneumoniae causes infections in humans especially in immunocompromised patients. About 80 % of nosocomial infections caused by K. pneumoniae are due to multidrug-resistant strains. The emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial therapies, which led our group to study the ability of bacterial viruses (known as bacteriophages or simply phages) to treat mice challenged with K. pneumoniae. Phage SS specific for K. pneumoniae B5055 was isolated and characterized, and its potential as a therapeutic agent was evaluated in an experimental model of K. pneumoniae-mediated lobar pneumonia in mice. Mice were challenged by intranasal (i.n.) inoculation with bacteria (108 c.f.u. ml−1). A single intraperitoneal injection of 1010 p.f.u. ml−1 phage administered immediately after i.n. challenge was sufficient to rescue 100 % of animals from K. pneumoniae-mediated respiratory infections. Administration of the phage preparation 3 h prior to i.n. bacterial challenge provided significant protection in infected mice, while even 6 h delay of phage administration after the induction of infection rendered the phage treatment ineffective. The results of this study therefore suggest that the timing of starting the phage therapy after initiation of infection significantly contributes towards the success of the treatment.

scholarly journals Study on antibacterial activities of Hibiscus rosa-sinensis L. Leaf and flower extracts against Proteus mirabilis, Pseudomonas aeruginosa, and Klebsiella pneumoniae

2019 ◽  
Vol 2 (1) ◽  
pp. 19-26
Author(s):  
Luong Thi My Ngan ◽  
Le Thi Kim Lan ◽  
Nguyen Thi Thuy Linh ◽  
Nguyen Ngoc Quy ◽  
Le Thi Thanh Loan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Proteus Mirabilis ◽  
Higher Plants ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Bacterial Strains ◽  
Tract Infections

Antibiotic resistance of bacterial strains causing serious diseases is one of the major concerns of public health worldwide. Metabolites of plants, particularly higher plants, have been suggested as alternative potential sources for antibacterial products due to their safe. They have little or no side effects and may act at multiple and novel target sites to bacteria. The study aims to evaluate antibacterial activities of leaf and flower extracts and solvent soluble fractions of the extracts against Proteus mirabilis, Pseudomonas aeruginosa, and Klebsiella pneumoniae, the major causes of infection-related kidney stones (struvite stones). Diameters of inhibitory zones, and MIC and MBC values of the extracts and fractions against the bacteria were evaluated. The results showed that the extracts and fractions derived from flowers have activities stronger than those from leaves, especially the ethyl acetate fraction (EtOAc fr.). The inhibitory zone diameters of 10 mg per paper disc of the EtOAC fr. towards Ps. aeruginosa, Pr. mirabilis, and K. pneumoniae were 17, 15 and 13 mm, respectively. The EtOAC fraction. had antibacterial activity against both Ps. aeruginosa and Pr. mirabilis with MIC (MBC) values of 2.5–5.0 (7.5) mg/mL and against K. pneumoniae with MIC (MBC) values of 7.5 (10) mg/mL. The fraction needs to be more studied for identifying its major active constituents. These leaf and flower extracts of H. rosa-sinensis could be used to treat against urinary tract infections caused by multiple drug resistant bacteria.

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