scholarly journals Host Antiviral Responses against Avian Infectious Bronchitis Virus (IBV): Focus on Innate Immunity

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1698
Author(s):  
Yun Zhang ◽  
Zhichao Xu ◽  
Yongchang Cao
Keyword(s):  
Innate Immunity ◽  
Infectious Bronchitis Virus ◽  
Vaccine Development ◽  
Innate Immune ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Innate Immune Responses ◽  
Infectious Bronchitis ◽  
Antiviral Responses ◽  
Virus Interaction

Avian infectious bronchitis virus (IBV) is an important gammacoronavirus. The virus is highly contagious, can infect chickens of all ages, and causes considerable economic losses in the poultry industry worldwide. In the last few decades, numerous studies have been published regarding pathogenicity, vaccination, and host immunity-virus interaction. In particular, innate immunity serves as the first line of defense against invasive pathogens and plays an important role in the pathogenetic process of IBV infection. This review focuses on fundamental aspects of host innate immune responses after IBV infection, including identification of conserved viral structures and different components of host with antiviral activity, which could provide useful information for novel vaccine development, vaccination strategies, and intervention programs.

scholarly journals Near-Complete Genome Sequence of GI-17 Lineage Infectious Bronchitis Virus, Circulating in Iowa

2021 ◽  
Vol 10 (20) ◽  
Author(s):  
Amro Hashish ◽  
Yuko Sato ◽  
Ganwu Li ◽  
Ying Zheng ◽  
Phillip C. Gauger ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Genome Sequence ◽  
Causative Agent ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Poultry Industry ◽  
Infectious Bronchitis

ABSTRACT Avian infectious bronchitis virus (AvIBV) is the causative agent of a highly contagious respiratory disease in chickens which results in significant economic losses in the poultry industry. Here, we report a near-complete genome sequence of the strain, designated IA1162/2020, identified in tracheal swabs from chickens in Iowa in 2020.

scholarly journals A Novel Immunochromatographic Strip for Antigen Detection of Avian Infectious Bronchitis Virus

2019 ◽  
Vol 20 (9) ◽  
pp. 2216 ◽  
Author(s):  
I-Li Liu ◽  
Yi-Chun Lin ◽  
Yong-Chong Lin ◽  
Cai-Zhen Jian ◽  
Ivan-Chen Cheng ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Antigenic Variation ◽  
Viral Evolution ◽  
Rapid Test ◽  
High Specificity ◽  
Antigen Detection ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Immunochromatographic Strip ◽  
Infectious Bronchitis

Avian infectious bronchitis virus (IBV) causes considerable economic losses in the poultry industry worldwide, including Taiwan. IBV is among the most important pathogens in chickens, and it spreads rapidly among flocks. In addition to dozens of known serotypes, new viral variants have emerged due to the viral evolution and antigenic variation in IBVs. Therefore, the development of a sensitive, specific, and easily performed assay is crucial for the rapid detection and surveillance of IBV infections. A rapid and simple immunochromatographic strip (ICS) was developed in this study by employing monoclonal antibodies against spike and nucleocapsid proteins of IBV as the tracer and the capture antibody. The ICS showed high specificity in detecting IBV antigens, including several IBV genotypes and novel variants, as opposed to three other common avian respiratory viruses. The detection limit of the strip reached 104.4 50% embryo-infective dose. Moreover, in the experimental chicken model, the strip test demonstrated consistency in detecting IBV with RT-PCR gene detection. Taken together, this antigen detection strip has the potential to serve as an on-farm rapid test for IBV; therefore, it may facilitate surveillance and control of the disease.

scholarly journals GENETIC CHARACTERIZATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES RECOVERED IN CIS COUNTRIES IN 2015–2017

2018 ◽  
pp. 30-39 ◽  
Author(s):  
L. O. Scherbakova ◽  
S. N. Kolosov ◽  
Z. B. Nikonova ◽  
N. G. Zinyakov ◽  
Ye. V. Ovchinnikova ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Hypervariable Region ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Genetic Lineages ◽  
Infectious Bronchitis ◽  
Poultry Farms ◽  
Cis Countries ◽  
In Cis ◽  
Virus Isolates

Avian infectious bronchitis virus is a cause of major economic losses in poultry industry. However, control of the virus is very complicated due to its high variability. The mutation frequency in the hypervariable region of the S1 gene of the virus isolated from the vaccinated birds annually amounts to 1.5%. Long-term observations of the circulation of IBV isolates detected in a number of poultry farms demonstrated that the virus genetic lineages circulating on the poultry farms could eventually change. This stipulates the need for the continuous monitoring of the virus isolates for the prevention schedule optimization. The paper demonstrates test results of 840 biological samples collected from chickens on the poultry farms in Russia and some CIS countries in 2015–2017. From 311 positive samples 147 IBV isolates were recovered, the majority of which belonged to eight genetic lines of GI genotype: GI-1, GI-12, GI-13, GI-14, GI-16, GI-19, GI-22, GI-23. Moreover, recombinant isolates were detected as well as variant isolates that belonged to none of the known genotypes.

scholarly journals Negative Regulation of RNF90 on RNA Virus-Triggered Antiviral Immune Responses Targeting MAVS

2021 ◽  
Vol 12 ◽  
Author(s):  
Bo Yang ◽  
Ge Zhang ◽  
Xiao Qin ◽  
Yulu Huang ◽  
Xiaowen Ren ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Immune Responses ◽  
Rna Virus ◽  
Ring Finger ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Antiviral Signaling ◽  
Antiviral Responses ◽  
Mitochondrial Antiviral Signaling ◽  
Antiviral Innate Immunity

The antiviral innate immunity is the first line of host defense against viral infection. Mitochondrial antiviral signaling protein (MAVS, also named Cardif/IPS-1/VISA) is a critical protein in RNA virus-induced antiviral signaling pathways. Our previous research suggested that E3 ubiquitin-protein ligases RING-finger protein (RNF90) negatively regulate cellular antiviral responses by targeting STING for degradation, though its role in RNA virus infection remains unknown. This study demonstrated that RNF90 negatively regulated RNA virus-triggered antiviral innate immune responses in RNF90-silenced PMA-THP1 cells, RNF90-deficient cells (including HaCaTs, MEFs, and BMDMs), and RNF90-deficient mice. However, RNF90 regulated RNA virus-triggered antiviral innate immune responses independent of STING. RNF90 promoted K48-linked ubiquitination of MAVS and its proteasome-dependent degradation, leading to the inhibition of innate immune responses. Altogether, our findings suggested a novel function and mechanism of RNF90 in antiviral innate immunity.

scholarly journals First Complete Genome Sequence of Currently Circulating Infectious Bronchitis Virus Strain DMV/1639 of the GI-17 Lineage

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Iryna V. Goraichuk ◽  
Arun B. Kulkarni ◽  
Dawn Williams-Coplin ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Broiler Chickens ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis

Avian infectious bronchitis virus is the causative agent of a highly contagious disease that results in severe economic losses to the poultry industry worldwide. Here, we report the first coding-complete genome sequence of strain DMV/1639 of the GI-17 lineage, isolated from broiler chickens in Georgia in 2019.

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

scholarly journals Design and Characterization of a DNA Vaccine Based on Spike with Consensus Nucleotide Sequence against Infectious Bronchitis Virus

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Lei Zuo ◽  
Wenjun Yan ◽  
Zhou Song ◽  
Hao Li ◽  
Xin Xie ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dna Vaccine ◽  
Infectious Bronchitis Virus ◽  
Neutralizing Antibody ◽  
Economic Losses ◽  
Post Translational Modification ◽  
Virus Shedding ◽  
Infectious Bronchitis ◽  
Specific Pathogen ◽  
Cellular Immune

Avian coronavirus infectious bronchitis virus (IBV) causes severe economic losses in the poultry industry, but its control is hampered by the continuous emergence of new genotypes and the lack of cross-protection among different IBV genotypes. We designed a new immunogen based on a spike with the consensus nucleotide sequence (S_con) that may overcome the extraordinary genetic diversity of IBV. S_con was cloned into a pVAX1 vector to form a new IBV DNA vaccine, pV-S_con. pV-S_con could be correctly expressed in HD11 cells with corresponding post-translational modification, and induced a neutralizing antibody response to the Vero-cell-adapted IBV strain Beaudette (p65) in mice. To further evaluate its immunogenicity, specific-pathogen-free (SPF) chickens were immunized with the pV-S_con plasmid and compared with the control pVAX1 vector and the H120 vaccine. Detection of IBV-specific antibodies and cell cytokines (IL-4 and IFN-γ) indicated that vaccination with pV-S_con efficiently induced both humoral and cellular immune responses. After challenge with the heterologous strain M41, virus shedding and virus loading in tissues was significantly reduced both by pV-S_con and its homologous vaccine H120. Thus, pV-S_con is a promising vaccine candidate for IBV, and the consensus approach is an appealing method for vaccine design in viruses with high variability.

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