scholarly journals Genomic Epidemiology and Evolution of Duck Hepatitis A Virus

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1592
Author(s):  
Enikő Fehér ◽  
Szilvia Jakab ◽  
Krisztina Bali ◽  
Eszter Kaszab ◽  
Borbála Nagy ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Evolutionary Mechanisms ◽  
Protein Coding ◽  
Genomic Epidemiology ◽  
Coding Regions ◽  
Duck Hepatitis ◽  
Rates Of Evolution ◽  
Genomic Regions ◽  
Globally Distributed

Duck hepatitis A virus (DHAV), an avian picornavirus, causes high-mortality acute disease in ducklings. Among the three serotypes, DHAV-1 is globally distributed, whereas DHAV-2 and DHAV-3 serotypes are chiefly restricted to Southeast Asia. In this study, we analyzed the genomic evolution of DHAV-1 strains using extant GenBank records and genomic sequences of 10 DHAV-1 strains originating from a large disease outbreak in 2004–2005, in Hungary. Recombination analysis revealed intragenotype recombination within DHAV-1 as well as intergenotype recombination events involving DHAV-1 and DHAV-3 strains. The intergenotype recombination occurred in the VP0 region. Diversifying selection seems to act at sites of certain genomic regions. Calculations estimated slightly lower rates of evolution of DHAV-1 (mean rates for individual protein coding regions, 5.6286 × 10−4 to 1.1147 × 10−3 substitutions per site per year) compared to other picornaviruses. The observed evolutionary mechanisms indicate that whole-genome-based analysis of DHAV strains is needed to better understand the emergence of novel strains and their geographical dispersal.

scholarly journals Adaptation of primate cell-adapted hepatitis A virus strain HM175 to growth in guinea pig cells is independent of mutations in the 5′ nontranslated region

2001 ◽  
Vol 82 (3) ◽  
pp. 597-602 ◽  
Author(s):  
Werner Frings ◽  
Andreas Dotzauer
Keyword(s):  
Guinea Pig ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Growth Efficiency ◽  
Wild Type Virus ◽  
Specific Information ◽  
Protein Coding ◽  
Coding Regions ◽  
Nontranslated Region ◽  
Primate Cell

Previous studies of hepatitis A virus (HAV) genotypes after adaptation of wild-type virus to growth in cell cultures of primate origin identified determinants for growth in cell culture in the viral 2B and 2C protein-coding regions of the genome and demonstrated that an increased growth efficiency in a particular cell line was achieved by subsequent mutations in the 5′ nontranslated region (5′NTR). The results reported in this study demonstrate that the passage of HAV adapted to primate BS-C-1 cells in guinea pig cells resulted in increased growth efficiency in the rodent cells and decreased growth efficiency in BS-C-1 cells. This adaptation occurred without mutation in the 5′NTR, but the viral 2B and 2C proteins seem to play a role during adaptation to the new environment, as one mutation occurred in each protein. Although the data presented here do not clearly identify which region of the viral genome underwent mutations to improve the interaction of the viruses with guinea pig proteins, they do confirm that the 5′NTR is not the only region responsible for providing host cell-specific information.

scholarly journals A viroporin-like 2B protein of Duck Hepatitis A Virus 1that induces incomplete autophagy in DEF cells

2021 ◽  
pp. 101331
Author(s):  
Zezheng Liu ◽  
Qian Ye ◽  
Anchun. Cheng ◽  
Xuming. Ou ◽  
Sai Mao ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis ◽  
2B Protein

Comparative pathogenicity of different subtypes of duck hepatitis A virus in Pekin ducklings

2019 ◽  
Vol 228 ◽  
pp. 181-187 ◽  
Author(s):  
Rongchang Liu ◽  
Shaohua Shi ◽  
Yu Huang ◽  
Zhen Chen ◽  
Cuiteng Chen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis

scholarly journals Molecular epidemiology and genetic diversity of duck hepatitis A virus type 3 in Shandong province of China, 2012–2014

2017 ◽  
Vol 61 (04) ◽  
pp. 463-472 ◽  
Author(s):  
R. ZHANG ◽  
L. XIA ◽  
J. CHEN ◽  
Y. GONG ◽  
L. ZHANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Epidemiology ◽  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Shandong Province ◽  
Duck Hepatitis ◽  
Type 3

scholarly journals Construction of Recombinant Lactococcus lactis Strain Expressing VP1 Fusion Protein of Duck Hepatitis A Virus Type 1 and Evaluation of Its Immune Effect

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1479
Author(s):  
Xiaoting Zhang ◽  
Ruihua Zhang ◽  
Jingyu Wang ◽  
Nana Sui ◽  
Guige Xu ◽  
...  
Keyword(s):  
Immune System ◽  
Fusion Protein ◽  
Lactococcus Lactis ◽  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Interleukin 2 ◽  
Interleukin 4 ◽  
Duck Hepatitis

With the continuous development of duck farming and the increasing breeding density, the incidence of duck hepatitis A virus type 1 (DHAV-1) has been on the rise, seriously endangering the development of duck farming. To reduce the use of antibiotics in duck breeding, susceptibility risks and mortality, and avoid virulence recovery and immune failure risk, this study aims to develop a new type of mucosal immune probiotics and make full use of molecular biology techniques, on the level of genetic engineering, to modify Lactococcus lactis (L. lactis). In this study, a secretory recombinant L. lactis named MG1363-VP1 with an enhanced Green Fluorescent Protein (eGFP) and translation enhancer T7g10L was constructed, which could express the VP1-eGFP fusion protein of DHAV-1. The animal experiment in ducklings was performed to detect the immune response and protection effect of oral microecologics by recombinant L. lactis. The results showed that oral L. lactis MG1363-VP1 significantly induced the body’s humoral immune system and mucosal immune system to produce specific anti-VP1 IgG antibodies and mucosal secretory immunoglobulin A (sIgA) for DHAV-1 in ducklings, and cytokines including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10), and interferon gamma (IFN-γ). The mortality rate was monitored simultaneously by the natural infestation in the process of production and breeding; notably, the ducklings vaccinated with L. lactis MG1363-VP1 were effectively protected against the nature infection of DHAV-1. The recombinant L. lactis MG1363-VP1 constructed in this study provides a new means of preventing and controlling DHAV-1 infection in the future.

scholarly journals Duck Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent Cellular Translation Shutoff via PERK/GCN2

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanzhi Liu ◽  
Anchun Cheng ◽  
Mingshu Wang ◽  
Sai Mao ◽  
Xumin Ou ◽  
...  
Keyword(s):  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Translation Efficiency ◽  
Eif2α Phosphorylation ◽  
Duck Hepatitis ◽  
Cellular Translation ◽  
First Time

Duck hepatitis A virus type 1 (DHAV-1) is one of the most deadly pathogens that endanger the duck industry. Most viruses usually turn off host translation after infection to facilitate viral replication and translation. For the first time report to our knowledge, DHAV-1 can induce eIF2α phosphorylation and inhibit cellular translation in duck embryo fibroblasts (DEFs). Moreover, the activity of DHAV-1 in the cells caused obvious eIF2α phosphorylation, which has nothing to do with the viral protein. Subsequently, we screened two kinases (PERK and GCN2) that affect eIF2α phosphorylation through inhibitors and shRNA. Notably, the role of GCN2 in other picornaviruses has not been reported. In addition, when the phosphorylation of eIF2α induced by DHAV-1 is inhibited, the translation efficiency of DEFs restores to a normal level, indicating that DHAV-1 induced cellular translation shutoff is dependent on eIF2α phosphorylation.

scholarly journals Virologic and Immunologic Characteristics in Mature Ducks with Acute Duck Hepatitis A Virus 1 Infection

2017 ◽  
Vol 8 ◽  
Author(s):  
Sai Mao ◽  
Mingshu Wang ◽  
Xumin Ou ◽  
Di Sun ◽  
Anchun Cheng ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis
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