scholarly journals Laboratory Findings in Patients with Probable Dengue Diagnosis from an Endemic Area in Colombia in 2018

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1401
Author(s):  
Jenny C. Cardenas ◽  
Sandra Y. Giraldo-Parra ◽  
Maria U. Gonzalez ◽  
Lady Y. Gutierrez-Silva ◽  
Lucy Jaimes-Villamizar ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Rural Areas ◽  
Vaccine Development ◽  
Hematological Parameters ◽  
Emerging Diseases ◽  
Accurate Diagnosis ◽  
Correct Treatment ◽  
Laboratory Findings ◽  
Ns1 Antigen ◽  
Novel Coronavirus

As demonstrated with the novel coronavirus pandemic, rapid and accurate diagnosis is key to determine the clinical characteristic of a disease and to improve vaccine development. Once the infected person is identified, hematological findings may be used to predict disease outcome and offer the correct treatment. Rapid and accurate diagnosis and clinical parameters are pivotal to track infections during clinical trials and set protection status. This is also applicable for re-emerging diseases like dengue fever, which causes outbreaks in Asia and Latin America every 4 to 5 years. Some areas in the US are also endemic for the transmission of dengue virus (DENV), the causal agent of dengue fever. However, significant number of DENV infections in rural areas are diagnosed solely by clinical and hematological findings because of the lack of availability of ELISA or PCR-based tests or the infrastructure to implement them in the near future. Rapid diagnostic tests (RDT) are a less sensitive, yet they represent a timely way of detecting DENV infections. The purpose of this study was to determine whether there is an association between hematological findings and the probability for an NS1-based DENV RDT to detect the DENV NS1 antigen. We also aimed to describe the hematological parameters that are associated with the diagnosis through each test.

scholarly journals Laboratory and Radiologic Findings in Pediatrics with COVID-19: A Systematic Review

2020 ◽  
Vol 15 (3) ◽  
Author(s):  
Mehdi Shokri ◽  
Mohammad Karimian ◽  
Feizollah Mansouri ◽  
Somayeh Mahdikhani ◽  
Milad Borji ◽  
...  
Keyword(s):  
Systematic Review ◽  
Ct Scan ◽  
Children And Adolescents ◽  
Health Care Team ◽  
Emerging Diseases ◽  
Laboratory Findings ◽  
Team Members ◽  
Radiologic Findings ◽  
The Status ◽  
Novel Coronavirus

Context: One of the emerging diseases that has spread in 2019 is COVID-19. Objectives: The present systematic review (SR) was performed to evaluate the status of laboratory and radiological findings in the age group of children and adolescents with COVID-19. Methods: In this SR study, three of the research team members conducted searches in SCOPUS, PUBMED, Science Direct, and ISI international sites. The keywords that were searched included children- pediatric- adolescents- coronavirus- COVID-19- Novel coronavirus SARS-CoV-2-epidemiology- diagnostic findings- laboratory findings- CT SCAN- computed tomography- percentage of lymphocyte- alanine aminotransferase- white blood cell count (WBC). The combination of search keywords with AND as well as OR was performed. For analysis, descriptive data reporting was performed using EndNote software. Results: The analysis of the laboratory and radiology findings of 197 patients of 12 studies included in the SR study showed notable changes in the lung CT scan and laboratory ranges that confirm the COVID-19 infection in these patients. Conclusions: This systematic review provides information on the laboratory and radiology findings of children and adolescents with COVID-19 for the health care team.

scholarly journals The clinical and laboratory profile of dengue fever in a tertiary care hospital

2019 ◽  
Vol 6 (5) ◽  
pp. 1447
Author(s):  
Katari Sujitha ◽  
M. Dorasanamm ◽  
M. V. Nagabhushana
Keyword(s):  
Dengue Fever ◽  
Tertiary Care ◽  
Control Measures ◽  
Care Hospital ◽  
Monsoon Period ◽  
Laboratory Findings ◽  
Ns1 Antigen ◽  
Medical Wards ◽  
Considerable Morbidity ◽  
Atypical Presentations

Background: Dengue fever is one of the most common acute viral illness associated with considerable morbidity and mortality. Recently, there is an alarming rise of dengue in India. This study was undertaken to know the clinical profile and laboratory findings during the evolution of dengue fever.Methods: A total of 120 patients of dengue fever who were NS1 antigen or IgM dengue positive, admitted to medical wards of Narayana Medical College, Nellore included in the study. Thorough clinical examination and relevant laboratory investigations performed in all patientsResults: Males were commonly affected (72.4%). Fever was the most common presentation (100%), followed by headache (96%) myalgia (94%), abdominal pain (24.46%), and retro-orbital pain (12.45%). Malena was the most common hemorrhagic manifestation. Laboratory findings include varying degree of thrombocytopenia in all patients, leukocytopenia (30.52%), increased hematocrit (>45%) (67.59%) and deranged liver function test (58.33%)Conclusions: Early diagnosis, monitoring, and prompt supportive management can reduce mortality in dengue. Atypical presentations of dengue should be kept in mind, which may cause a delay in the diagnosis. Increased community awareness and vector control measures need to be strengthened during the peri-monsoon period to reduce the burden of dengue cases.

scholarly journals Evaluation of NS1, IgM ELISA and RT-PCR in diagnosis of dengue fever

2018 ◽  
Vol 6 (7) ◽  
pp. 2440
Author(s):  
Deepak Kumar ◽  
Rajesh Kumar Verma ◽  
Amit Singh ◽  
Manoj Kumar ◽  
Dharmendra Prasad Singh ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Late Phase ◽  
Accurate Diagnosis ◽  
Suspected Case ◽  
Rt Pcr ◽  
Igm Elisa ◽  
Antigen Elisa ◽  
Ns1 Antigen

Background: Dengue fever is a mosquito borne disease caused by flavivirus. Its cases are increasing in India with increasing mortality rate year by year hence, prompt and accurate diagnosis is necessary to prevent morbidity and mortality.Methods: In this study we enrolled 125 clinically suspected cases of dengue. All the collected samples were processed for RT-PCR, NS1 and IgM ELISA. We evaluated NS1 antigen ELISA alone, and combination of NS1 and IgM ELISA against RT-PCR.Results: Among 125 clinically suspected case 67 were positive by RT-PCR and 58 were negative. Sensitivity and Specificity of NS1 ELISA and NS1 with IgM ELISA (in combination) against RT-PCR were 83.58%, 94.82% and 95.55%, 79.31% respectively. (p<0.001).Conclusions: The NS1 ELISA alone was sufficient to detect acute phase of dengue fever, although, combination of NS1 and IgM proved to be most appropriate method for detection of acute as well as late phase of dengue fever.

scholarly journals Clinical Profile and Laboratory Findings of Dengue Fever in Children

2021 ◽  
Vol 11 (5) ◽  
pp. 30-37
Author(s):  
Mahesh Ahirrao ◽  
Ashish Patil ◽  
Anjali Kurup
Keyword(s):  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Clinical Profile ◽  
Health Concern ◽  
Age Group ◽  
Laboratory Findings ◽  
Igm Antibody ◽  
Ns1 Antigen

Background: Dengue is an arthropod borne viral hemorrhagic fever. It is a major public health concern throughout the tropical and subtropical regions of the world. Objectives: To study clinical profile and laboratory findings of dengue infection in children. Materials and Methods: Children up to the age of 15 years who were confirmed serologically dengue positive (either positive NS1 antigen or positive IgM antibody by ELISA method were included in study. Total 100 patients were studied. Pre tested, semi structured questionnaire were developed and used for data collection. Result and Discussion: In current study, maximum patients were found in Dengue Hemorrhagic Fever (DHF) group (68%), followed by Dengue Fever (25%). Fever was most common clinical manifestation. Maximum patients in this study were in 10 to 15 years of age group, male being more commonly affected. Thrombocytopenia and leukopenia were present in 90% and 69% of patients respectively. Conclusion: School going children were more exposed to mosquito bites during day time school activities, thus maximum patients were from 10 to 15 years of age group. In early diagnosis of dengue fever, ELISA NS1 antigen is seen more promisable. When used in combination with ELISA IgM antibody, it significantly improves the diagnostic algorithm. Thrombocytopenia is more consistent feature of Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Key words: Dengue Hemorrhagic Fever (DHF), Dengue Shock Syndrome (DSS), ELISA NS1 antigen, ELISA IgM antibody.

scholarly journals SARS-CoV-2/DENV co-infection: a series of cases from the Federal District, Midwestern Brazil

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Heidi Luise Schulte ◽  
José Diego Brito-Sousa ◽  
Marcus Vinicius Guimarães Lacerda ◽  
Luciana Ansaneli Naves ◽  
Eliana Teles de Gois ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Federal District ◽  
Therapeutic Interventions ◽  
Diagnostic Process ◽  
The Novel ◽  
Prevention Measures ◽  
Laboratory Findings ◽  
Novel Coronavirus ◽  
The Impact

Abstract Background Since the novel coronavirus disease outbreak, over 179.7 million people have been infected by SARS-CoV-2 worldwide, including the population living in dengue-endemic regions, particularly Latin America and Southeast Asia, raising concern about the impact of possible co-infections. Methods Thirteen SARS-CoV-2/DENV co-infection cases reported in Midwestern Brazil between April and September of 2020 are described. Information was gathered from hospital medical records regarding the most relevant clinical and laboratory findings, diagnostic process, therapeutic interventions, together with clinician-assessed outcomes and follow-up. Results Of the 13 cases, seven patients presented Acute Undifferentiated Febrile Syndrome and six had pre-existing co-morbidities, such as diabetes, hypertension and hypopituitarism. Two patients were pregnant. The most common symptoms and clinical signs reported at first evaluation were myalgia, fever and dyspnea. In six cases, the initial diagnosis was dengue fever, which delayed the diagnosis of concomitant infections. The most frequently applied therapeutic interventions were antibiotics and analgesics. In total, four patients were hospitalized. None of them were transferred to the intensive care unit or died. Clinical improvement was verified in all patients after a maximum of 21 days. Conclusions The cases reported here highlight the challenges in differential diagnosis and the importance of considering concomitant infections, especially to improve clinical management and possible prevention measures. Failure to consider a SARS-CoV-2/DENV co-infection may impact both individual and community levels, especially in endemic areas.

scholarly journals Psychological impact and implementation of preventative measures in hemodialysis centers during the COVID-19 pandemic: a provincial questionnaire survey in China

2021 ◽  
Author(s):  
Yuanhan Chen ◽  
Yanhua Wu ◽  
Penghua Hu ◽  
Xia Fu ◽  
Shuangxin Liu ◽  
...  
Keyword(s):  
Rural Areas ◽  
Questionnaire Survey ◽  
Medical Staff ◽  
Psychological Impact ◽  
Guangdong Province ◽  
Psychological Status ◽  
Staff Members ◽  
Preventative Measures ◽  
Tertiary Hospitals ◽  
Novel Coronavirus

Abstract Objectives This study investigated the psychological status of patients and staff, and the implementation of preventative measures in hemodialysis centers in Guangdong province, China, during the 2019 novel coronavirus disease (COVID-19) pandemic. Methods An electronic questionnaire survey was carried out anonymously between March 28 and April 3, 2020. All of the 516 hemodialysis centers registered in Guangdong province were invited to participate in the survey. The questionnaires were designed to investigate the psychological status of hemodialysis patients and general staff members (doctors, nurses, technicians, and other staff), and to address the implementation of preventative measures for administrators (directors or head nurses) of the hemodialysis centers. Results A total of 1782 patients, 3400 staff, and 420 administrators voluntarily participated in this survey. Patients living in rural areas reported a higher incidence of severe anxiety compared to those living in other areas (in rural areas, towns, and cities, the incidence rate was 17.0%, 9.0%, and 8.9%, respectively, P < 0.001). Medical staff were less likely to worry about being infected than non-medical staff (13.1% vs 30.3%, respectively, P < 0.001). With respect to the implementation of preventative measures, hemodialysis centers in general hospitals outperformed stand-alone blood purification centers, while tertiary hospitals outperformed hospitals of other levels. However, restrictions regarding the admission of non-resident patients were lower in tertiary hospitals than in other hospitals. In this situation, only one patient imported from Hubei province was diagnosed with COVID-19. Conclusions COVID-19 did not significantly affect the psychological status of most patients and medical staff members. Due to the implementation of comprehensive preventative measures, there were no cluster outbreaks of COVID-19 in hemodialysis centers. This provincial-level survey may provide referential guidance for other countries and regions that are experiencing a similar pandemic.

A COMPREHENSIVE STUDY ON ACUTE NON-TRAUMATIC ABDOMINAL EMERGENCIES

2021 ◽  
pp. 14-16
Author(s):  
Md. Quamar Zubair ◽  
A. K. Jha Suman
Keyword(s):  
Abdominal Pain ◽  
Acute Appendicitis ◽  
Surgical Intervention ◽  
Age Groups ◽  
Diagnostic Procedures ◽  
Accurate Diagnosis ◽  
Postoperative Patient ◽  
Correct Treatment ◽  
Abdominal Emergencies ◽  
Comprehensive Study

Abdominal pain is one of the most common reasons for visit to the emergency room. Acute appendicitis is the commonest cause. An accurate diagnosis is essential for the correct treatment, which in many cases will prevent the death of the patient. Mainstay of diagnosis is history and physical examination. If this information is inadequate to establish a diagnosis and urgent or immediate operation is unnecessary, the periodic re-examination helps document the progression of the disease and often avoids unnecessary surgical intervention. Today the combination of improved diagnostic procedures, antibiotic and better anaesthesia and preoperative and postoperative patient care has led to a decrease in morbidity and mortality of patients with acute abdomen. The objective of this study was to determine the various causes of nontraumatic acute abdominal emergencies, their incidence, management and mortality in both sexes and all age groups >12 years age.

Poisoning from wild mushrooms in Eastern Anatolia region: analyses of 5 years

2007 ◽  
Vol 26 (7) ◽  
pp. 579-582 ◽  
Author(s):  
P. Durukan ◽  
M. Yildiz ◽  
Y. Cevik ◽  
I. Ikizceli ◽  
C. Kavalci ◽  
...  
Keyword(s):  
Health Centre ◽  
Time Lag ◽  
Abdominal Discomfort ◽  
Mushroom Poisoning ◽  
Eastern Anatolia ◽  
Wild Mushroom ◽  
Wild Mushrooms ◽  
Correct Treatment ◽  
Laboratory Findings ◽  
Duration Of Hospitalization

The objective of this study was to describe the demographic and clinical features of patients who were admitted to the emergency department (ED) due to wild mushroom poisoning and to point the importance of mushroom poisonings in our area. This study was performed by examining the files of wild mushroom poisoning patients who were admitted to the ED of Firat University, Faculty of Medicine, between January 2000 and June 2004, retrospectively. Patients ≥16 years of age were included in the study. The frequency of wild mushroom poisoning, age and sex of the patients, season, place of the residence, laboratory findings, treatment and outcome of the patients were investigated. During the study period, 64 patients with wild mushroom poisoning were admitted to the ED. From 64 overall patients, 25 (39.1%) were males. The most common complaints during the admission were nausea, vomiting and abdominal discomfort. The duration of hospitalization was two (range 1—4 days) days. No death was observed. Severity of mushroom poisoning depends on the type of mushroom eaten, the time lag between the poisoning and admission to the hospital, and the rapid and correct treatment given to the patient either in the ambulance or at health centre. Human & Experimental Toxicology (2007) 26: 579—582.

scholarly journals Insights into Cross-species Evolution of Novel Human Coronavirus 2019-nCoV and Defining Immune Determinants for Vaccine Development

2020 ◽  
Author(s):  
Arunachalam Ramaiah ◽  
Vaithilingaraja Arumugaswami
Keyword(s):  
Binding Affinity ◽  
Vaccine Development ◽  
Asia Pacific ◽  
Potential Candidate ◽  
T Cell Epitopes ◽  
Live Animal ◽  
Synonymous Mutations ◽  
Wide Range ◽  
Novel Coronavirus ◽  
N Proteins

ABSTRACTNovel Coronavirus (nCoV) outbreak in the city of Wuhan, China during December 2019, has now spread to various countries across the globe triggering a heightened containment effort. This human pathogen is a member of betacoronavirus genus carrying 30 kilobase of single positive-sense RNA genome. Understanding the evolution, zoonotic transmission, and source of this novel virus would help accelerating containment and prevention efforts. The present study reported detailed analysis of 2019-nCoV genome evolution and potential candidate peptides for vaccine development. This nCoV genotype might have been evolved from a bat-CoV by accumulating non-synonymous mutations, indels, and recombination events. Structural proteins Spike (S), and Membrane (M) had extensive mutational changes, whereas Envelope (E) and Nucleocapsid (N) proteins were very conserved suggesting differential selection pressures exerted on 2019-nCoV during evolution. Interestingly, 2019-nCoV Spike protein contains a 39 nucleotide sequence insertion relative to SARS-like bat-SL-CoVZC45/2017. Furthermore, we identified eight high binding affinity (HBA) CD4 T-cell epitopes in the S, E, M and N proteins, which can be commonly recognized by HLA-DR alleles of Asia and Asia-Pacific Region population. These immunodominant epitopes can be incorporated in universal subunit CoV vaccine. Diverse HLA types and variations in the epitope binding affinity may contribute to the wide range of immunopathological outcomes of circulating virus in humans. Our findings emphasize the requirement for continuous surveillance of CoV strains in live animal markets to better understand the viral adaptation to human host and to develop practical solutions to prevent the emergence of novel pathogenic CoV strains.

scholarly journals DENGUE FEVER

2011 ◽  
Vol 18 (01) ◽  
pp. 57-63
Author(s):  
IRFAN ARSHAD ◽  
FAYYAZ AHMED MALIK ◽  
AAMIR HUSSAIN ◽  
Shahida A.R.Shah
Keyword(s):  
Dengue Fever ◽  
Blood Urea Nitrogen ◽  
White Cell Count ◽  
Hematological Parameters ◽  
Strong Association ◽  
Urea Nitrogen ◽  
Major Health ◽  
Prolonged Aptt ◽  
Common Clinical Presentation ◽  
Low Platelet Count

One of the major health hazards that has erupted in Pakistan within the recent past years and has caused loss of life of many young people is Dengue Fever. Objective: Main objective was to find clinico-pathologic parameters which are essentially associated with complications and contribute to the adverse outcome. Material & Methods: This prospective study was conducted on 106 seropositive cases of dengue fever. Patients were taken from Abbasi Shaheed Hospital, Karachi from June 2008 to March 2009. Results: The most common hematological abnormalities were thrombocytopenia and leucopenia. Platelets count below 50 × 103/ μL was seen in (78%) cases and 49 % patients had total white cell count below 4 ×103 /μL. Partial thromboplastin time was significantly prolonged in (26%) cases whereas prothrombin time was normal in all patients. Liver enzymes both Aspartate Aminotransferase (AST) and Alanine Amino-transferase were significantly elevated in (60%) patients. Blood urea nitrogen (BUN) and creatinine was deranged in (23%) patients. Conclusions: Fever was the most common clinical presentation( 86 % of the patients). Hematological parameters (low platelet count, low total leucocytes count, prolonged APTT and raised hematocrit) and biochemical parameters (raised aminotransferases, blood urea nitrogen) have strong association with the complications of dengue fever and hence are associated with the poor outcome of disease. 

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