scholarly journals Severe Fever with Thrombocytopenia Syndrome in Cats and Its Prevalence among Veterinarian Staff Members in Nagasaki, Japan

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1142
Author(s):  
Tsuyoshi Ando ◽  
Takeshi Nabeshima ◽  
Shingo Inoue ◽  
Mya Myat Ngwe Tun ◽  
Miho Obata ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Phylogenetic Analyses ◽  
The Body ◽  
Virus Growth ◽  
Specific Antibodies ◽  
Staff Members ◽  
Zoonotic Risk ◽  
Sentinel Animals ◽  
Animal Care ◽  
Severe Fever

In this study, we investigated severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) infection in cats in Nagasaki, Japan. In total, 44 of 133 (33.1%) cats with suspected SFTS were confirmed to be infected with SFTSV. Phylogenetic analyses of SFTSV isolates from cats indicated that the main genotype in Nagasaki was J1 and that unique reassortant strains with J2 (S segment) and unclassified genotypes (M and L segments) were also present. There were no significant differences in virus growth in cell cultures or fatality in SFTSV-infected mice between the SFTSV strains that were isolated from recovered and fatal cat cases. Remarkably, SFTSV RNAs were detected in the swabs from cats, indicating that the body fluids contain SFTSV. To evaluate the risk of SFTSV infection when providing animal care, we further examined the seroprevalence of SFTSV infection in veterinarian staff members; 3 of 71 (4.2%) were seropositive for SFTSV-specific antibodies. Our results provide useful information on the possibility of using cats as sentinel animals and raised concerns of the zoonotic risk of catching SFTSV from animals.

Experimental Production of the Bi-Digital O-Ring Test Muscular Power Evaluation Device Using an Air-type Automatic Analysis System

2020 ◽  
Vol 45 (1) ◽  
pp. 15-30
Author(s):  
Yasuhiro Shimotsuura ◽  
Hiroyuki Maezawa ◽  
Yoshiaki Omura
Keyword(s):  
New York ◽  
Muscle Strength ◽  
Objective Evaluation ◽  
The Body ◽  
Ring Test ◽  
Experimental Production ◽  
Muscular Power ◽  
Strong Response ◽  
Staff Members ◽  
Diagnosis Method

As Bi-Digital O-Ring Test (originated and founded by Prof. Y. Omura in New York, 1997-2020; follow as BDORT)is a diagnosis method that is carried out on the basic theory of the physiological phenomenon called the decline of muscular power of fingers, the examiner, and patients (or mediator) are demanded to do BDORT by constant regular power. Namely BDORT is a diagnosis method that estimates the relative muscular decline of the patients, so there is such a view that the results of BDORT are reflected by consciousness of the examiner. The authors used the ORT tester by using air system to avoid the influence of electromagnetic wave and evaluated the decline of the muscle strength and open degree of the O-ring shaped by the patients. Patients of the Shimotsuura Clinic are subjected and checked by direct BDORT method. When the patients shapes the O-Ring, staff members stimulated the parts of the body by plastic stick and push foot switch. Decline of the muscle strength & open degree was evaluated. When the open degree was more than 20%, stimulated points were evaluated as abnormal. Opposite side arm of the O-Ring shaped arm was checked as control. The results of the direct BDORT method between ORT evaluation apparatus and the patient was consistent with the results of the indirect method of BDORT method between the doctor and the assistant. Even where the patients complain of ill, the muscle strength was declined and opened the O-Ring by using ORT evaluation apparatus. Especially in the parts of the strong response of Integrin α5β1 checked by the doctor, the muscle strength decreased and the open degree was much higher than other parts of the body. Patients could experience of BDORT by numeral objective evaluation of the decline of the muscle strength by using ORT evaluation apparatus.

scholarly journals Efficiency and Teamwork in Emergency Departments: Perception of Staff on Design Interventions

2021 ◽  
pp. 193758672110231
Author(s):  
Sahar Ahmadpour ◽  
Sara Bayramzadeh ◽  
Parsa Aghaei
Keyword(s):  
Work Environment ◽  
Emergency Departments ◽  
Online Survey ◽  
The Body ◽  
Healthcare Outcomes ◽  
Design Strategies ◽  
Design Features ◽  
Staff Members ◽  
Enhance Efficiency ◽  
Efficient Work

Objectives: The goal of this study is twofold: (1) identifying design strategies that enhance efficiency and support teamwork in emergency departments (EDs) and (2) identifying design features that contribute to the spread of COVID-19, based on staff perception. Background: Due to increasing ED visits annually, an efficient work environment has become one of the main concerns in designing EDs. According to the literature, an efficient work environment and teamwork improve healthcare outcomes and positively impact staff satisfaction. During the COVID-19 pandemic, EDs faced various changes such as workflow and space usage. Few studies explored staff perceptions about the influence of design features on efficiency, teamwork, and the COVID-19 spread. Method: An online survey with 14 open- and closed-ended questions was distributed among ED staff members to collect data about unit design features that impact efficiency, teamwork, and the COVID-19 spread. Results: The central nursing station was one of the preferred configurations that increased efficiency and teamwork in EDs. Decentralizing disposal rooms in small-size EDs and decentralizing the medication room in large-size EDs with more than 65 exam rooms can decrease staff walking steps. Flexibility to expand treatment spaces on demand, one-way track circulation path, and changing the air pressure in COVID-19 treatment areas were some of the staff suggestions for future EDs. Conclusion: The findings of this study contribute to the body of knowledge that EDs’ physical environments can impact efficiency and teamwork among staff and, consequently, healthcare outcomes. Compartmentalization of the ED layout can reduce the spread of COVID-19.

scholarly journals Redescription of Paradiplozoon hemiculteri (Monogenea, Diplozoidae) from the type host Hemiculter leucisculus, with neotype designation

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 4 ◽  
Author(s):  
Dagmar Jirsová ◽  
Xuejuan Ding ◽  
Kristína Civáňová ◽  
Eliška Jirounková ◽  
Jana Ilgová ◽  
...  
Keyword(s):  
Southern China ◽  
Phylogenetic Analyses ◽  
Posterior Part ◽  
Original Description ◽  
Molecular Data ◽  
The Body ◽  
Type Host ◽  
Hemiculter Leucisculus ◽  
Morphological And Molecular Data ◽  
Median Plate

Paradiplozoon hemiculteri (Ling, 1973), a member of the Diplozoidae, parasitizes the gills of Asian fish. Not only is the type material unavailable for this species, the original description was poor and somewhat conflicting, and adequate molecular data were not available. What is more, the available morphological and molecular data are inconsistent and fluctuate significantly. Here, we present a redescription of P. hemiculteri based on morphological and molecular data from new isolates collected from the type host, the sharpbelly Hemiculter leucisculus (Basilewsky, 1855), captured at the neotype locality (Shaoguan, Guangdong Province, southern China); a neotype for P. hemiculteri was designated from this collection. The length and width of the body, buccal suckers, pharynx, attachment clamps, sickle and the central hook handle were all measured and the shape of the anterior and posterior part of the median plate and anterior and posterior joining sclerites accurately documented. Phylogenetic analyses based on the sequences of the second rDNA internal transcribed spacer (ITS2) indicated that all new samples clustered together and differed clearly from sequences attributed to P. hemiculteri, which are deposited in GenBank. Our results confirm that P. hemiculteri is the only diplozoid that has demonstrably been found on the gills of H. leucisculus to date.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

Investigating Benzene and Metabolite Mediated Alterations in Nrf2 Signaling in Murine Fetal Liver Cells

2018 ◽  
Author(s):  
Enoch Lam
Keyword(s):  
Free Radicals ◽  
Cell Cultures ◽  
Childhood Leukemia ◽  
Fetal Liver ◽  
White Blood Cells ◽  
Chemical Exposure ◽  
The Body ◽  
Chain Reactions ◽  
Additional Cell ◽  
Other Substances

Leukemia is a cancer of the blood, where the body produces an overabundance of immature white blood cells. It is the most common childhood cancer and possibly originates from chemical exposure during fetal development. Understanding the molecular mechanism and causes of childhood leukemia will help the development of therapeutic and preventative strategies to reduce and treat occurrences of this cancer. Benzene is a non-polar aromatic ring that has been confirmed to cause leukemia in adults. Benzene metabolites can generate free radicals that could potentially be involved in the development of childhood leukemia through in utero exposures. It is found in tobacco smoke, gasoline, industrial solvents and many other substances. Pregnant mothers can be chronically exposed to these substances through daily activities. In order to help determine the mechanism of benzene toxicity, a study was performed to determine the presence of free radicals in the livers of fetal mice at gestation day 14. Fetal liver cell cultures were exposed to varying concentrations of benzene and additional cell cultures were exposed to various concentrations of a benzene metabolite mixture, composed of hydroquinone and benzoquinone. RNA was extracted from these cells and converted into complementary DNA. cDNA was then used in quantitative polymerase chain reactions to analyze a set of genes that are regulated by an antioxidant pathway. This pathway can be activated in response to oxidative stress, and a change in gene expression would indicate the presence of benzene or benzene metabolite toxicity in the liver at gestation day 14.

Taxonomic description and 3D modelling of a new species of myzostomid (Annelida, Myzostomida) associated with black corals from Madagascar

Zootaxa ◽  
2017 ◽  
Vol 4244 (2) ◽  
pp. 277 ◽  
Author(s):  
LUCAS TERRANA ◽  
IGOR EECKHAUT
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Three Dimensional ◽  
Close Relation ◽  
Ventral Side ◽  
Middle Part ◽  
The Body ◽  
Taxonomic Description ◽  
Black Corals ◽  
Organ Systems

Eenymeenymyzostoma nigrocorallium n. sp. is the first species of myzostomid worm associated with black corals to be described. Endoparasitic specimens of E. nigrocorallium were found associated with three species of antipatharians on the Great Reef of Toliara. Individuals inhabit the gastrovascular ducts of their hosts and evidence of infestation is, most of the time, not visible externally. Phylogenetic analyses based on 18S rDNA, 16S rDNA and COI data indicate a close relation to Eenymeenymyzostoma cirripedium, the only other species of the genus. The morphology of E. nigrocorallium is very unusual compared to that of the more conventional E. cirripedium. The new species has five pairs of extremely reduced parapodia located on the body margin and no introvert, cirri or lateral organs. Individuals are hermaphroditic, with the male and female gonads both being located dorsally in the trunk. It also has a highly developed parenchymo-muscular layer on the ventral side, and the digestive system lies in the middle part of the trunk. A three-dimensional digital model of this worm’s body plan has been constructed whereby the external morphology and in toto views of the observed organ systems (nervous, digestive and reproductive) can be viewed on-screen: http://doi.org/10.13140/RG.2.2.17911.21923. 

scholarly journals Capripoxviruses: Exploring the genetic relatedness between field and vaccine strains from Egypt

2019 ◽  
Vol 12 (12) ◽  
pp. 1924-1930
Author(s):  
Sherin Reda Rouby ◽  
Abdel-Hamid Bazid ◽  
Momtaz Wasfy ◽  
Magdy El-Sayed
Keyword(s):  
Cell Cultures ◽  
Vaccine Strain ◽  
Virus Isolation ◽  
Genetic Relatedness ◽  
Phylogenetic Analyses ◽  
Heart Cell ◽  
Fetal Kidney ◽  
Field Isolates ◽  
Gpcr Gene ◽  
Ovine Fetal

Background and Aim: Lumpy skin disease (LSD) and sheep pox are economically important Capripoxvirus-induced diseases of cattle and sheep, respectively. Despite the extensive vaccination program adopted by Egyptian veterinary authorities, LSD and sheep pox are still prevalent and spread throughout the whole country. The current study was designed for molecular characterization and phylogenetic analysis of LSD virus (LSDV) and Sheep pox virus (SPPV) recovered from field cases in Egypt along with vaccinal strains to assess their genetic relatedness. Materials and Methods: Skin biopsies were collected from naturally infected cases of LSD in Ismailia (n=3 farms) and Beni-Suef (n=2 farms) Governorates and sheep pox in Beni-Suef (n=1 flock). Virus isolation was carried out on primary ovine fetal kidney and heart cell cultures. DNA was extracted from infected materials (skin lesions, infected cell cultures) as well as LSDV Neethling vaccine strain and Romanian SPPV vaccine strain. Polymerase chain reaction was performed using oligonucleotide primers targeting the entire open reading frame of G protein-coupled receptors (GPCR) gene and gene sequences were analyzed. Results: Virus isolation on primary ovine fetal kidney and heart cell culture revealed a cytopathic effect at the third passage characterized by rounding of infected cells and margination of nuclear chromatin. Comparative sequence analysis of GPCR gene revealed that Egyptian LSDV isolated from Ismailia and Beni-Suef shared 99:100% nucleotide and amino acid (AA) identities with each other. In comparison to the vaccinal strains, Egyptian LSDV isolates shared 98:99 nucleotide and AA identities with LSDV Neethling vaccine strain and 93:94% with SPPV Romanian vaccine strain. No differences at the nucleotide or AAs were observed between the SPPV vaccine and virulent strains (100% identity). Phylogenetic analyses revealed that LSDV Neethling vaccine strain is more related to field Egyptian LSDV and clustered within the LSDV group while Romanian SPPV vaccine strain clustered in a separate clade with SPPV field isolates. Conclusion: Comparative sequencing and phylogenetic analyses of the GPCR gene reveal a minimal genetic variation between LSDV field isolates from different locations and a close relationship between virulent field strains and homologous vaccines.

scholarly journals Human resources department in crisis: A case of a university in South Africa

2014 ◽  
Vol 10 (3) ◽  
pp. 19-27
Author(s):  
Bethuel Sibongiseni Ngcamu
Keyword(s):  
Higher Education ◽  
South Africa ◽  
Human Resources ◽  
Higher Education Institutions ◽  
The Body ◽  
Staff Members ◽  
Management Interventions ◽  
Department Staff ◽  
University Leaders ◽  
Depth Interviews

Human Resources (HR) departments in previously disadvantaged higher education institutions (HEI) in South Africa have contributed to the crisis that has led them to be governed by appointed Administrators assigned by the Ministry of Higher Education and Training. The malfunctioning of HR departments persists even in the post-Administrator’s era, with a prevalence of personal, interpersonal and operational challenges. This study aimed at interrogating challenges faced by the HR Department of the university concerned post the appointed Administrator era and its negative effects. This study adopted a qualitative research approach whereby in-depth interviews were conducted with a sample of 10 HR Department staff members. Focus group discussions were also carried out with 20 academic and non-academic university leaders. Further data was also collected through ethnographic observation and secondary data was also used. The non-probability purposive sampling was used for qualitative analysis and NVivo was used for organizing and analyzing data gleaned from the in-depth interviews. The study findings consistently revealed unclear roles and responsibilities, outmoded recruitment and selection processes and poor interpersonal relations amongst the HR department staff members as some of the challenges that still persist post the Administrator’s era, all of which have contributed to the paralysis of organizational culture. University leaders can use the study findings as a tool to devise and implement radical change management interventions aiming at re-engineering HR departments that are operating abnormally, as well as for designing a responsive HR Strategic Architect. This study will contribute to the body of knowledge in the HR fraternity as there is a dearth of published studies on the internal challenges faces by HR Departments (both interpersonal and operational), which have the potential to hamper smooth operations of higher education institutions.

Pregnancy and velvet

2021 ◽  
Vol 65 (04) ◽  
pp. 86-88
Author(s):  
Sevinc Nadir qızı Kerimova ◽  
Keyword(s):  
Immune Response ◽  
Laboratory Test ◽  
Key Words ◽  
Rubella Virus ◽  
The Body ◽  
Key Words Pregnancy ◽  
Specific Antibodies ◽  
Rubella Infection

The main prevention of rubella infection during pregnancy is to be vaccinated against this disease in preparation for pregnancy. Before you decide to get vaccinated against rubella, you need to have a special laboratory test to detect antibodies to the rubella virus in your blood to check if you are immune to this infection. The fact is that in some cases it is impossible to determine whether you are sick with rubella. Because in many cases, the disease can be latent or with a very limited number of symptoms, in which case, naturally, the body develops specific antibodies against the virus. Doctors believe that in this case, the body's immune response will be strengthened. It is recommended that the velvet vaccine be given at least 3 months before the planned date of pregnancy. Key words: pregnancy, rubella, fetus, infection

Role of a hospital accreditation program in developing a process management system

2019 ◽  
Vol 32 (1) ◽  
pp. 120-137
Author(s):  
Erfan Shakibaei
Keyword(s):  
Process Management ◽  
The Body ◽  
Successful Implementation ◽  
Structured Interviews ◽  
Hospital Accreditation ◽  
Content Type ◽  
Staff Members ◽  
Positive Effects ◽  
Accreditation Program ◽  
Private Charity

PurposeThe purpose of this paper is to clarify the effects of the Iranian Hospital Accreditation Program (IHAP) on hospital processes from the viewpoint of the staff charged with establishing the program.Design/methodology/approachThis qualitative study is based on the data collected in semi-structured interviews conducted in 2016, which involved eight questions. Interviews were held with 70 staff members at 14 hospitals. Managerial staff were purposively interviewed based on their familiarity and involvement with the program. The hospitals were divided into five groups, comprising public, private, charity, military and social service hospitals. A thematic analysis was carried out using the collected data.FindingsThree themes emerged from the data, which together comprise a process management cycle: the establishment, implementation, and control phases of the program. For each phase, various positive trends, as well as hurdles for establishing the program, declared which were framed two sub-themes as positive effects and challenges.Originality/valueThe findings contribute to the body of evidence used by policy-makers and hospital managers to improve the change management processes related to the Iranian IHAP. Although positive changes in the process management cycles at Iranian hospitals were noted, successful implementation of the program demands a thorough assessment of the hospitals’ technical and financial needs (taking into account disparities between hospitals), and there is an urgent requirement for a plan to meet these needs.

Sign in / Sign up

Export Citation Format

Share Document