scholarly journals Circovirus in Blood of a Febrile Horse with Hepatitis

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 944
Author(s):  
Alvin Hui ◽  
Eda Altan ◽  
Nathan Slovis ◽  
Caitlin Fletcher ◽  
Xutao Deng ◽  
...  
Keyword(s):  
New Species ◽  
Real Time ◽  
Genome Sequence ◽  
Real Time Pcr ◽  
Sanger Sequencing ◽  
Viral Metagenomics ◽  
Nucleotide Identity ◽  
Wide Range ◽  
Asymptomatic Infections ◽  
A New Species

Circoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circoviruses were recognized. PCR identified the circovirus reads as originating from a pregnant mare with fever and hepatitis. That horse’s serum was also positive by real time PCR for equine parvovirus H and negative for the flavivirus equine hepacivirus. The complete circular genome of equine circovirus 1 strain Charaf (EqCV1-Charaf) was completed using PCR and Sanger sequencing. EqCV1 replicase showed 73–74% identity to those of their closest relatives, pig circoviruses 1/2, and elk circovirus. The closest capsid proteins were from the same ungulate circoviruses with 62–63% identity. The overall nucleotide identity of 72% to its closest relative indicates that EqCV1 is a new species in the Circovirus genus, the first reported in genus Equus. Whether EqCV1 alone or in co-infections can result in disease and its prevalence in different equine populations will require further studies now facilitated using EqCV1′s genome sequence.

scholarly journals Draft Genome Sequence of Bacillus albus Strain IB84, Isolated from Mexican Soil

2021 ◽  
Vol 10 (17) ◽  
Author(s):  
Jorge H. García-García ◽  
Carlos J. Moreno-Peña ◽  
Cesar I. Hernández-Vásquez ◽  
Luis J. Galán-Wong ◽  
Benito Pereyra-Alférez
Keyword(s):  
New Species ◽  
Bacillus Thuringiensis ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Nucleotide Identity ◽  
Average Nucleotide Identity ◽  
Gene Sequences ◽  
Content Type ◽  
A New Species

ABSTRACT Bacillus albus is a new species, but it lies on the borderline with Bacillus thuringiensis. In this work, we report a strain previously identified as Bacillus thuringiensis IB84, which now, based on average nucleotide identity and rRNA 16S, gyrB, groEL, and xre gene sequences, must be identified as Bacillus albus.

A new species of Sclerocephalus with a fully ossified endocranium gives insight into braincase evolution in temnospondyls

2021 ◽  
pp. 1-13
Author(s):  
Rainer R. Schoch ◽  
Gabriela Sobral
Keyword(s):  
New Species ◽  
Character Evolution ◽  
Late Paleozoic ◽  
New Taxon ◽  
Early Permian ◽  
Top Predator ◽  
Central European ◽  
Wide Range ◽  
A New Species ◽  
Insight Into

Abstract The late Paleozoic temnospondyl Sclerocephalus formed an aquatic top predator in various central European lakes of the late Carboniferous and early Permian. Despite hundreds of specimens spanning a wide range of sizes, knowledge of the endocranium (braincase and palatoquadrate) remained very insufficient in Sclerocephalus and other stereospondylomorphs because even large skulls had unossified endocrania. A new specimen from a stratigraphically ancient deposit at St. Wendel in southwestern Germany is recognized as representing a new taxon, S. concordiae new species, and reveals a completely ossified endocranium. The sphenethmoid was completely ossified from the basisphenoid to the anterior ethmoid region, co-ossified with the parasphenoid, and the basipterygoid joint was fully established. The pterygoid bears a slender, S-shaped epipterygoid, which formed a robust pillar lateral to the braincase. The massive stapes was firmly sutured to the parasphenoid. In the temnospondyl endocranium, character evolution involved various changes in the epipterygoid region, which evolved distinct morphologies in each of the major clades. UUID: http://zoobank.org/5e6d2078-eacf-4467-84cf-a12efcae7c0b

A new species of genus Hexacentrus Serville, 1831 from Taiwan (Orthoptera: Tettigoniidae: Hexacentrinae)

Zootaxa ◽  
2021 ◽  
Vol 4933 (4) ◽  
pp. 543-556
Author(s):  
PO-WEI CHEN ◽  
HUI-CONG XIE ◽  
XUE WU ◽  
CHU-ZE SHEN ◽  
ZHU-QING HE
Keyword(s):  
New Species ◽  
Gene Tree ◽  
Species Level ◽  
National Museum ◽  
Type Specimens ◽  
As Species ◽  
Wide Range ◽  
Similar Appearance ◽  
One New Species ◽  
A New Species

There are 29 species or subspecies in genus Hexacentrus occurring in Asia, Africa and Australia. Because of its similar appearance, it is not easy to distinguish them by traditional methods. In this study, we collected samples and sequenced COI genes from wide range. By reconstructing the gene tree, we found one new species, H. formosanus Chen et He sp. nov., from Taiwan. The new species is similar to H. expansus or H. inflatissimus, but differs from the former in male Cu2 vein of left tegmina curved and slender, and spectrum of male left tegmina slender and subsquare; differs from the later by body size smaller and female tegmina narrow and short. The type specimens are deposited in National Museum of Natural Science, Taichung, Taiwan (NMNS). H. japonicus hareyamai is treated as species level, H. hareyamai stat. nov. 

A new species of Aethiopella Handschin, 1942 (Collembola, Poduromorpha, Neanuridae) from Neotropical Region, with comments on the genus

Zootaxa ◽  
2019 ◽  
Vol 4629 (1) ◽  
pp. 39-50 ◽  
Author(s):  
RAIANE VITAL DA PAZ ◽  
GABRIEL COSTA QUEIROZ ◽  
BRUNO CAVALCANTE BELLINI
Keyword(s):  
New Species ◽  
Current Knowledge ◽  
Antennal Segment ◽  
Neotropical Region ◽  
The Other ◽  
Wide Range ◽  
Morphological Differences ◽  
A New Species

Aethiopella Handschin, 1942 as other Pseudachorutinae (Neanuridae) presents a diagnosis with overlapping characters when compared to other similar genera, especially Halachorutes Arlé, 1966, Ceratrimeria Börner, 1906 and Pseudachorutes Tullberg, 1871. This condition, combined to several gaps on the knowledge of described species and a wide range of morphological differences within the genus, reinforces the need of its revision, since it can be an artificial group. In spite of that, a new species, Aethiopella ricardoi sp. nov., is described and illustrated. It resembles the other five Neotropical taxa of Aethiopella but is unique by the combination of fourth antennal segment with trilobed apical bulb and 7 dorsal sensilla, lack of dorsolateral S-microchaeta (ms); postantennal organ with 13–19 vesicles; mandibles with 7–8 teeth; maxillae apically hooked; and tibiotarsi I–III with 19/19/18 chaetae, respectively, with M chaeta slightly basally displaced. We also present an updated diagnosis of Aethiopella based on current knowledge of Pseudachorutinae, a table comparing all species, a key to Neotropical taxa and remarks on the genus. 

The dog-faced water snakes, a revision of the genus Cerberus Cuvier, (Squamata, Serpentes, Homalopsidae), with the description of a new species

Zootaxa ◽  
2012 ◽  
Vol 3484 (1) ◽  
pp. 1 ◽  
Author(s):  
JOHN C. MURPHY ◽  
HAROLD K. VORIS ◽  
DARYL R. KARNS
Keyword(s):  
New Species ◽  
South Asian ◽  
Salt Water ◽  
Type Locality ◽  
New Combination ◽  
Salt Tolerant ◽  
Wide Range ◽  
Molecular Studies ◽  
Water Snakes ◽  
A New Species

Snakes of the genus Cerberus Cuvier, 1829 occupy a unique, widespread coastal distribution and have a salt-tolerant physiology that allows members of the genus to move across a wide range of salinities from full salt water to freshwater. Cerberus nomenclature is revised based upon morphology and builds on previous molecular studies. Three species have been recognized by recent workers, here we recognize five species: the South Asian C. rynchops (Schneider 1799); the Southeast Asian-Philippine C. schneiderii (Schlegel 1837), a new combination; the freshwater Philippine endemic C. microlepis Boulenger 1896; the Australopapuan C. australis (Gray 1842); and a new species from Micronesia. We also select a lectotype for Homalopsis schneiderii Schlegel based upon a figure published in 1837 and restrict the type locality for this species to Timor. Evidence is also presented for a population of Cerberus australis in Indonesia, west of Weber’s Line.

scholarly journals Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Runa M Grimholt ◽  
Petter Urdal ◽  
Olav Klingenberg ◽  
Armin P Piehler
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Globin Gene ◽  
Copy Number Variations ◽  
Globin Genes ◽  
Quantitative Real Time Pcr ◽  
Human Genetic Disease ◽  
Large Deletions ◽  
Wide Range

Abstract Background Alpha-thalassemia is the most common human genetic disease worldwide. Copy number variations in the form of deletions of α-globin genes lead to α-thalassemia while duplications of α-globin genes can cause a severe phenotype in β-thalassemia carriers due to accentuation of globin chain imbalance. It is important to have simple and reliable methods to identify unknown or rare deletions and duplications in cases in which thalassemia is suspected but cannot be confirmed by multiplex gap-PCR. Here we describe a copy number variation assay to detect deletions and duplications in the α-globin gene cluster (HBA-CNV). Results Quantitative real-time PCR was performed using four TaqMan® assays which specifically amplify target sequences representing both the α-globin genes, the –α3.7 deletion and the HS-40 region. The copy number for each target was determined by the 2-ΔΔCq method. To validate our method, we compared the HBA-CNV method with traditional gap-PCR in 108 samples from patients referred to our laboratory for hemoglobinopathy evaluation. To determine the robustness of the four assays, we analyzed samples with and without deletions diluted to obtain different DNA concentrations. The HBA-CNV method identified the correct copy numbers in all 108 samples. All four assays showed the correct copy number within a wide range of DNA concentrations (3.2-100 ng/μL), showing that it is a robust and reliable method. By using the method in routine diagnostics of hemoglobinopathies we have also identified several deletions and duplications that are not detected with conventional gap-PCR. Conclusions HBA-CNV is able to detect all known large deletions and duplications affecting the α-globin genes, providing a flexible and simple workflow with rapid and reliable results.

scholarly journals Draft Genome Sequence of Pelagicola sp. Strain LXJ1103, Isolated from Coastal Surface Seawater

2018 ◽  
Vol 7 (14) ◽  
Author(s):  
Dexi Zhao ◽  
Jia Li ◽  
Shan Lin ◽  
Xuejiao Liang ◽  
Xiaopei Lin ◽  
...  
Keyword(s):  
New Species ◽  
Genome Sequence ◽  
Surface Waters ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Surface Seawater ◽  
Content Type ◽  
The Family ◽  
A New Species

Pelagicola sp. strain LXJ1103, a representative of a new species in the family Rhodobacteraceae, was isolated from the coastal surface waters in Xiamen, China.

scholarly journals Complete Genome Sequence of a Boa (Boa constrictor)-Specific Papillomavirus Type 1 Isolate

2018 ◽  
Vol 7 (16) ◽  
Author(s):  
Jakub Kubacki ◽  
Anna Sophie Ramsauer ◽  
Claudia Bachofen ◽  
Claude Favrot ◽  
Alexandra Nicolier ◽  
...  
Keyword(s):  
New Species ◽  
Nucleotide Sequence ◽  
Genome Sequence ◽  
Skin Lesions ◽  
Nucleotide Sequence Analysis ◽  
Content Type ◽  
Boa Constrictor ◽  
Full Length Genome ◽  
A New Species

We present the full-length genome sequence of a new papillomavirus detected in skin lesions collected from a boa (Boa constrictor). Based on the nucleotide sequence analysis, we propose to designate the newly identified virus as Boa constrictor papillomavirus type 1 (BcPV1), a new species in the genus Dyomupapillomavirus.

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