scholarly journals The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 606
Author(s):  
Lidong Wang ◽  
Yanyan Zhang ◽  
Teng Chen ◽  
Lijuan Mi ◽  
Xuefei Sun ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Field Trial ◽  
Subunit Vaccine ◽  
Recombinant Baculovirus ◽  
Vector System ◽  
Control Group ◽  
Virus Shedding ◽  
Average Litter Size ◽  
Baculovirus Expression Vector ◽  
A Subunit

Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressing the capsid protein was constructed using the baculovirus expression vector system (BEVS). A subunit vaccine was developed based on the capsid protein with appropriate adjuvant. Then, a field trial was carried out in two districts in order to evaluate the efficiency of the subunit vaccine. The field trial indicated that in total, only 1.8% of the minks developed typical diarrhea in the vaccinated group compared with 74.5% in the control group. The vaccination could significantly reduce the infection rate of MiCV among the mink herds and could restrain the virus’ shedding from feces. Furthermore, the vaccinated group had a higher average litter size in the following year compared to the control group. Collectively, the results indicated that the subunit vaccine based on the capsid protein can provide reliable protection against MiCV infection.

scholarly journals A Newly Designed EGFP-2A Peptide Monocistronic Baculoviral Vector for Concatenating the Expression of Recombinant Proteins in Insect Cells

Processes ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 291 ◽  
Author(s):  
Chih-Yu Wu ◽  
Chao-Wei Huang ◽  
Yu-Shin Nai ◽  
Pei-Yu Chu ◽  
Chung-Hsiung Wang ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Posttranslational Modifications ◽  
Mammalian Cells ◽  
Insect Cells ◽  
Fluorescent Protein ◽  
Expression System ◽  
Recombinant Baculovirus ◽  
New Combination ◽  
Vector System ◽  
Baculovirus Expression Vector

Recombinant proteins produced by the baculovirus expression vector system (BVES) have been widely applied in the agricultural and medical fields. However, the procedure for protein expression is inefficient and needs to be improved. Herein, we propose a simple construct that incorporates a selectable marker (enhanced green fluorescent protein, EGFP) and a picorna viral-derived “self-cleaving” 2A-like peptide to separate the EGFP and target proteins in a monocistronic baculovirus vector to facilitate isolation of the recombinant baculovirus in the BVES. In this study, porcine adiponectin (ADN), a secreted, multimeric protein with insulin-sensitizing properties, was used to demonstrate its utility in our EGFP-2A-based expression system. EGFP and ADN were simultaneously expressed by a recombinant alphabaculovirus. Co-expression of EGFP facilitates the manipulation of the following processes, such as determining expression kinetics and harvesting ADN. The results showed that the 2A “self-cleaving” process does not interfere with EGFP activity or with signal peptide removal and the secretion of recombinant ADN. Posttranslational modifications, including glycosylation, of the recombinant ADN occurred in insect cells, and the formation of various multimers was further verified. Most importantly, the insect-produced ADN showed a similar bioactivity to that of mammalian cells. This concept provides a practical and economic approach that utilizes a new combination of alphabaculovirus/insect cell expression systems for future applications.

scholarly journals Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

2012 ◽  
Vol 19 (5) ◽  
pp. 740-745 ◽  
Author(s):  
André A. Grassmann ◽  
Samuel R. Félix ◽  
Carolina Ximendes dos Santos ◽  
Marta G. Amaral ◽  
Amilton C. P. Seixas Neto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Subunit Vaccine ◽  
Lethal Dose ◽  
Control Group ◽  
Hamster Model ◽  
Content Type ◽  
B Subunit ◽  
Heat Labile ◽  
A Subunit

ABSTRACTLeptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species ofLeptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of theEscherichia coliheat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD50) ofLeptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P< 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

Recombinant rotavirus outer capsid protein a subunit vaccine

1998 ◽  
Vol 149 (1) ◽  
pp. 93-94
Author(s):  
D.K. Kang ◽  
P-H. Kim ◽  
E-J. Ko ◽  
S.Y. Seong ◽  
Y.H. Kim ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Subunit Vaccine ◽  
Protein A ◽  
Outer Capsid Protein ◽  
A Subunit ◽  
Outer Capsid

scholarly journals Development of Recombinant Dihydrolipoamide Dehydrogenase Subunit Vaccine against Vibrio Infection in Large Yellow Croaker

Fishes ◽  
2022 ◽  
Vol 7 (1) ◽  
pp. 17
Author(s):  
Xiaomeng Li ◽  
Yuanzhen Tan ◽  
Zheng Zhang ◽  
Yupeng Huang ◽  
Pengfei Mu ◽  
...  
Keyword(s):  
Subunit Vaccine ◽  
Vaccine Development ◽  
Head Kidney ◽  
Economic Losses ◽  
Control Group ◽  
Large Yellow Croaker ◽  
Dihydrolipoamide Dehydrogenase ◽  
A Subunit ◽  
And Control ◽  
Vibrio Genus

Large yellow croaker (Larimichthys crocea), an economically important marine fish in China, has suffered from serious vibriosis, which has resulted in great economic losses for the large yellow croaker industry. Vaccination has been considered to be a safe and effective method to prevent and control vibriosis. However, due to the complex diversity and serotypes of the Vibrio genus, the progress of Vibrio vaccine development is still slow. In this study, we prepared recombinant Vibrio dihydrolipoamide dehydrogenase (rDLD) protein and investigated its potential as a candidate to be a subunit vaccine against Vibrio. The lysozyme activity and the rDLD-specific antibody level in sera of large yellow croakers immunized with rDLD were significantly higher than those in the control group, and the transcript levels of proinflammatory cytokines (IL-6, IL-8, IL-1β), MHC IIα/β, CD40, CD8α, IL-4/13A, and IL-4/13B were significantly up-regulated in the spleen and head kidney of large yellow croakers immunized with rDLD, suggesting that rDLD could induce both specific and nonspecific immune responses in this species. In addition, rDLD protein increased the survival rate of large yellow croakers against Vibrio alginolyticus and Vibrio parahaemolyticus, with the relative percent of survival (RPS) being 74.5% and 66.9%, respectively. These results will facilitate the development of a potential subunit vaccine against Vibrio in large yellow croaker aquaculture.

Localization of the cystic fibrosis gene product in a transfected Sf9 insect cell line

1991 ◽  
Vol 49 ◽  
pp. 90-91
Author(s):  
C.A. Ackerley ◽  
N. Kartner ◽  
J.R. Riordan ◽  
M.J. Phillips
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Gene Product ◽  
Insect Cell Line ◽  
Recombinant Baculovirus ◽  
Vector System ◽  
Cystic Fibrosis Gene ◽  
Baculovirus Expression Vector System ◽  
Baculovirus Expression ◽  
Baculovirus Expression Vector

The cystic fibrosis gene product (CFTR) is thought to be either directly or indirectly involved with Cl- conductance in epithelial cells in which it is produced. Utilizing the Baculovirus expression vector system, CFTR has been expressed in Sf9 insect cells which in turn display a regulated Cl- conductance employing the same techniques as those used to demonstrate Cl- conductance in epithelial cells. This was not observed in mock-infected Bgalactosidase producing or non-infected Sf9 cells.Two cell lines, one infected with CFTR expressing recombinant baculovirus and the other infected with B-galactosidase expressing recombinant baculovirus were fixed in 2% paraformaldehyde and 0.001% glutaraldehyde in 0.1 M HEPES buffer, PH7.2 for 2 hours. They were then rinsed several times with buffer, infiltrated with 2% agarose and infused with 2.3 M sucrose for at least 24 hours. The samples were then frozen by rapid immersion in liquid nitrogen cooled FREON 22 and ultrathin cryosections cut with a cryoultramicrotome and immunogold labelled with purified monclonal antibodies against CFTR (1) using the method described by Griffiths.

A subunit vaccine against hydropericardium syndrome using adenovirus penton capsid protein

Vaccine ◽  
2012 ◽  
Vol 30 (50) ◽  
pp. 7153-7156 ◽  
Author(s):  
M.S. Shah ◽  
A. Ashraf ◽  
M. Rahman ◽  
M.I. Khan ◽  
J.A. Qureshi
Keyword(s):  
Capsid Protein ◽  
Subunit Vaccine ◽  
Hydropericardium Syndrome ◽  
A Subunit

scholarly journals Recombinant Baculovirus: A Flexible Drug Screening Platform for Chikungunya Virus

2021 ◽  
Vol 22 (15) ◽  
pp. 7891
Author(s):  
Muhammed Muhsin Varikkodan ◽  
Chun-Chung Chen ◽  
Tzong-Yuan Wu
Keyword(s):  
Chikungunya Virus ◽  
Fluorescent Protein ◽  
Recombinant Baculovirus ◽  
Infectious Agent ◽  
High Rate ◽  
Vector System ◽  
Structural Protein ◽  
Entry Site ◽  
Baculovirus Expression Vector

Chikungunya virus (CHIKV) is a mosquito-transmitted infectious agent that causes an endemic or epidemic outbreak(s) of Chikungunya fever that is reported in almost all countries. This virus is an intense global threat, due to its high rate of contagion and the lack of effective remedies. In this study, we developed two baculovirus expression vector system (BEVS)-based approaches for the screening of anti-CHIKV drugs in Spodoptera frugiperda insect (Sf21) cells and U-2OS cells. First, structural protein of CHIKV was co-expressed through BEVS and thereby induced cell fusion in Sf21 cells. We used an internal ribosome entry site (IRES) to co-express the green fluorescent protein (EGFP) for identifying these fusion events. The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form syncytia. We identified that ursolic acid has potential anti-CHIKV activity in vitro, by using this approach. Second, BacMam virus-based gene delivery has been successfully applied for the transient expression of non-structural proteins with a subgenomic promoter-EGFP (SP-EGFP) cassette in U-2OS cells to act as an in vitro CHIKV replicon system. Our BacMam-based screening system has identified that the potential effects of baicalin and baicalein phytocompounds can inhibit the replicon activity of CHIKV in U-2OS cells. In conclusion, our results suggested that BEVS can be a potential tool for screening drugs against CHIKV.

scholarly journals Evaluation of a Subunit H5 Vaccine and an Inactivated H5N2 Avian Influenza Marker Vaccine in Ducks Challenged with Vietnamese H5N1 Highly Pathogenic Avian Influenza Virus

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Tze-Hoong Chua ◽  
Connie Y. H. Leung ◽  
H. E. Fang ◽  
Chun-Kin Chow ◽  
Siu-Kit Ma ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Influenza Vaccine ◽  
Avian Influenza Virus ◽  
Recombinant Baculovirus ◽  
Antibody Responses ◽  
Small Scale ◽  
Virus Shedding ◽  
Marker Vaccine ◽  
A Subunit

The protective efficacy of a subunit avian influenza virus H5 vaccine based on recombinant baculovirus expressed H5 haemagglutinin antigen and an inactivated H5N2 avian influenza vaccine combined with a marker antigen (tetanus toxoid) was compared with commercially available inactivated H5N2 avian influenza vaccine in young ducks. Antibody responses, morbidity, mortality, and virus shedding were evaluated after challenge with a Vietnamese clade 1 H5N1 HPAI virus [A/VN/1203/04 (H5N1)] that was known to cause a high mortality rate in ducks. All three vaccines, administered with water-in-oil adjuvant, provided significant protection and dramatically reduced the duration and titer of virus shedding in the vaccinated challenged ducks compared with unvaccinated controls. The H5 subunit vaccine was shown to provide equivalent protection to the other two vaccines despite the H5 antibody responses in subunit vaccinated ducks being significantly lower prior to challenge. Ducks vaccinated with the H5N2 marker vaccine consistently produced antitetanus toxoid antibody. The two novel vaccines have attributes that would enhance H5N1 avian influenza surveillance and control by vaccination in small scale and village poultry systems.

scholarly journals Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)

2014 ◽  
Vol 64 (4) ◽  
pp. 493-509
Author(s):  
Prodanov-Radulović Jasna ◽  
Došen Radoslav ◽  
Polaček Vladimir ◽  
Petrović Tamaš ◽  
Stojanov Igor ◽  
...  
Keyword(s):  
Subunit Vaccine ◽  
Classical Swine Fever ◽  
Pathological Examination ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Active Immunisation ◽  
Challenge Virus ◽  
Virus Shedding ◽  
Serological Examination ◽  
A Subunit

Abstract The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions.

Development and efficacy of a grass carp reovirus (GCRV) outer capsid protein VP7 subunit vaccine

2012 ◽  
Vol 37 (6) ◽  
pp. 659-664 ◽  
Author(s):  
Shi-ying XU ◽  
Jing-hui LI ◽  
Yong ZOU ◽  
Lin LIU ◽  
Cheng-liang GONG ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Grass Carp ◽  
Subunit Vaccine ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Outer Capsid
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