scholarly journals Six Cases of Zika/Dengue Coinfection in a Brazilian Cohort, 2015–2019

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1201
Author(s):  
Claudio Siqueira ◽  
Valéria Féres ◽  
Livia Coutinho ◽  
Isabela Junqueira ◽  
Luziane Bento ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Outcomes ◽  
Acute Infection ◽  
Warning Signs ◽  
Surveillance Systems ◽  
Rt Pcr ◽  
Zikv Infection ◽  
Central Brazil ◽  
Ns1 Antigen ◽  
Coinfected Patient

Brazil is one of the countries which has been most affected by dengue epidemics. This scenario became more challenging with the emergence of Zika virus after 2014. The cocirculation of dengue and Zika viruses makes their diagnosis and treatment a challenge for health professionals, especially due to their similar clinical outcomes. From 2015 to 2019, we followed a cohort of 2017 participants in Goiania, Goias, Central Brazil. Febrile cases were monitored weekly, and after identification of fever, the physician performed a home visit for clinical evaluation and collection of blood/urine for diagnosis of acute dengue/Zika infection in suspected cases. Dengue acute infection was investigated by NS1 antigen and real time RT-PCR and seroconversion of anti-dengue IgM. ZIKV infection was confirmed by real time RT-PCR. Six cases of Zika/dengue coinfection among participants were reported. The clinical outcomes were suggestive for both DENV and ZIKV infection. No coinfected patient had neurological clinical manifestation, warning signs or need for hospitalization. A continuous specific laboratory confirmation for both dengue and Zika viruses should be enforced as part of the surveillance systems even in the presence of very suggestive cases of dengue fever, minimizing the risk of a late detection of ZIKV circulation.

Early diagnosis of dengue in travelers: Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology

2010 ◽  
Vol 47 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Eili Huhtamo ◽  
Essi Hasu ◽  
Nathalie Y. Uzcátegui ◽  
Elina Erra ◽  
Simo Nikkari ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Antigen Detection ◽  
Rt Pcr ◽  
Ns1 Antigen

scholarly journals Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1164 ◽  
Author(s):  
Alex Pauvolid-Corrêa ◽  
Helver Gonçalves Dias ◽  
Laura Marina Siqueira Maia ◽  
Grasiela Porfírio ◽  
Thais Oliveira Morgado ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Whole Blood ◽  
Nucleotide Sequencing ◽  
Human Populations ◽  
Envelope Gene ◽  
Rt Pcr ◽  
Central Brazil ◽  
West Central ◽  
Virus Serotype

Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT90). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.

scholarly journals A Cluster of Dengue Cases in Travelers: A Clinical Series From Thailand

2021 ◽  
Author(s):  
Hisham A Imad ◽  
Juthamas Phadungsombat ◽  
Emi E Nakayama ◽  
Lapakorn Chatapat ◽  
Phimphan Pitusan ◽  
...  
Keyword(s):  
Mosquito Species ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Warning Signs ◽  
Rt Pcr ◽  
Clinical Series ◽  
Serotype 1 ◽  
Ns1 Antigen ◽  
The Mean ◽  
Global Travel

Dengue is an overlooked tropical disease for which billions of people are at risk. The disease, caused by a Flavivirus with four distinct serotypes, is transmitted primarily by urbanized Aedes mosquito species. The infection leads to a spectrum of clinical manifestations, with the majority being asymptomatic. Primary dengue and, to a greater extent, subsequent infection, mainly secondary dengue infection, are associated with increased severity. Increased global travel and recreational tourism expose naïve individuals to dengue, the most common arboviral infections in travelers. We describe a cluster of possible primary acute dengue infections in a group of 12 individuals who presented to Bangkok Hospital for Tropical Diseases in 2017. Infection was confirmed by dengue NS1 antigen and multiplex real-time RT-PCR. Nine individuals required hospitalization, and four developed dengue warning signs. The mean arterial pressure was significantly lower in the group with dengue warning signs. The period from the day of arrival in Thailand and the first day of symptoms was significantly shorter in adolescents with warning signs. Leukocytes, neutrophils, and platelets declined significantly at defervescence and were negatively correlated with day of illness. Six clinical isolates were identified as dengue serotype-1, with identical sequences suggesting that these patients were infected with the same virus.

scholarly journals Surveillance of congenital Zika syndrome in England and Wales: methods and results of laboratory, obstetric and paediatric surveillance

2019 ◽  
Vol 147 ◽  
Author(s):  
C. Oeser ◽  
E. Aarons ◽  
P.T. Heath ◽  
K. Johnson ◽  
A. Khalil ◽  
...  
Keyword(s):  
National Laboratory ◽  
Acute Infection ◽  
Rapid Assessment ◽  
Surveillance Systems ◽  
Zikv Infection ◽  
Clinical Presentations ◽  
Congenital Zika Syndrome ◽  
Returning Travellers ◽  
Adverse Pregnancy ◽  
The Uk

Abstract The spread of the Zika virus (ZIKV) in the Americas led to large outbreaks across the region and most of the Southern hemisphere. Of greatest concern were complications following acute infection during pregnancy. At the beginning of the outbreak, the risk to unborn babies and their clinical presentation was unclear. This report describes the methods and results of the UK surveillance response to assess the risk of ZIKV to children born to returning travellers. Established surveillance systems operating within the UK – the paediatric and obstetric surveillance units for rare diseases, and national laboratory monitoring – enabled rapid assessment of this emerging public health threat. A combined total of 11 women experiencing adverse pregnancy outcomes after possible ZIKV exposure were reported by the three surveillance systems; five miscarriages, two intrauterine deaths and four children with clinical presentations potentially associated with ZIKV infection. Sixteen women were diagnosed with ZIKV during pregnancy in the UK. Amongst the offspring of these women, there was unequivocal laboratory evidence of infection in only one child. In the UK, the number and risk of congenital ZIKV infection for travellers returning from ZIKV-affected countries is very small.

scholarly journals A Cluster of Dengue Cases in Travelers: A Clinical Series from Thailand

2021 ◽  
Vol 6 (3) ◽  
pp. 152
Author(s):  
Hisham A. Imad ◽  
Juthamas Phadungsombat ◽  
Emi E. Nakayama ◽  
Lapakorn Chatapat ◽  
Phimphan Pisutsan ◽  
...  
Keyword(s):  
Mosquito Species ◽  
Clinical Manifestations ◽  
Warning Signs ◽  
Aedes Mosquito ◽  
Rt Pcr ◽  
Subsequent Infection ◽  
Clinical Series ◽  
Serotype 1 ◽  
Ns1 Antigen ◽  
Global Travel

Dengue is an overlooked tropical disease for which billions of people are at risk. The disease, caused by a Flavivirus with four distinct serotypes, is transmitted primarily by urban Aedes mosquito species. The infection leads to a spectrum of clinical manifestations, with the majority being asymptomatic. Primary dengue fever and, to a greater extent, a subsequent infection with a different serotype is associated with increased severity. Increased global travel and recreational tourism expose individuals naïve to the dengue viruses, the most common arboviral infections among travelers. We describe a cluster of possible primary acute dengue infections in a group of 12 individuals who presented to Bangkok Hospital for Tropical Diseases in 2017. Infection was confirmed by dengue NS1 antigen and multiplex real-time RT-PCR. Nine individuals required hospitalization, and four developed dengue warning signs. Leukocytes, neutrophils, and platelets declined towards defervescence and were negatively correlated with day of illness. Six clinical isolates were identified as dengue serotype-1, with 100% nucleotide identity suggesting that these patients were infected with the same virus.

Zika virus infection in asymptomatic persons in Myanmar, 2018

2020 ◽  
Vol 114 (6) ◽  
pp. 440-447
Author(s):  
Mya Myat Ngwe Tun ◽  
Saw Wut Hmone ◽  
Aung Min Soe ◽  
Elizabeth Luvai ◽  
Khine Mya Nwe ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Pacific Islands ◽  
Neutralization Test ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Zikv Infection ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Background Zika virus (ZIKV) is a mosquito-borne flavivirus. Outbreaks of ZIKV infection have occurred in Africa, Southeast Asia, the Pacific Islands, the Americas and the Caribbean. Although most ZIKV infections are asymptomatic, cases of neurological manifestations have been described. The aim of the present study was to identify the prevalence of ZIKV infection among the asymptomatic persons in Myanmar in 2018. Methods A total of 284 serum samples from apparently healthy persons were collected from Yangon, Myanmar in 2018. They were analysed for ZIKV infection by immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), IgG indirect ELISA, 50% focus reduction neutralization test, real-time reverse transcription polymerase chain reaction (RT-PCR) and conventional RT-PCR. Results Of the 284 apparently healthy persons, 31.3% were positive for the presence of IgM against ZIKV and 94.3% were positive for anti-flavivirus IgG. Among the ZIKV IgM-positive samples, we confirmed ZIKV infection in 15.8% of asymptomatic persons by neutralization test and real-time RT-PCR. Conclusions We conclude that ZIKV infection was increasing among asymptomatic persons in the same area in Myanmar during 2018 compared with 2017. It is highly recommended to strengthen the surveillance system for ZIKV to prevent possible outbreaks.

scholarly journals Molecular surveillance of Dengue in Sukabumi, West Java province, Indonesia

2014 ◽  
Vol 8 (06) ◽  
pp. 733-741 ◽  
Author(s):  
Roy Nusa ◽  
Heni Prasetyowati ◽  
Febrina Meutiawati ◽  
Benediktus Yohan ◽  
Hidayat Trimarsanto ◽  
...  
Keyword(s):  
Real Time ◽  
Evolutionary Rates ◽  
Genotype Distribution ◽  
Rt Pcr ◽  
West Java ◽  
Genotype I ◽  
Dengue Disease ◽  
Denv Serotypes ◽  
Ns1 Antigen ◽  
Serological Data

Introduction: Dengue is endemic and affects people in all Indonesian provinces. Increasing dengue cases have been observed every year in Sukabumi in West Java province. Despite the endemicity, limited data is available on the genetic of dengue viruses (DENV) circulating in the country. To understand the dynamics of dengue disease, we performed molecular and serological surveillance of dengue in Sukabumi. Methodology: A total of 113 patients were recruited for this study. Serological data were obtained using anti-dengue IgM and IgG tests plus dengue NS1 antigen detection. Dengue detection and serotyping were performed using real-time RT-PCR. Viruses were isolated and the envelope genes were sequenced. Phylogenetic and evolutionary analyses were performed to determine the genotype of the viruses and their evolutionary rates. Results: Real-time RT-PCR detected DENV in 25 (22%) of 113 samples. Serotyping revealed the predominance of DENV-2 (16 isolates, 64%), followed by DENV-1 (5 isolates, 20%), and DENV-4 (4 isolates, 16%). No DENV-3 was detected in the samples. Co-circulation of genotype I and IV of DENV-1 was observed. The DENV-2 isolates all belonged to the Cosmopolitan genotype, while DENV-4 isolates were grouped into genotype II. Overall, their evolutionary rates were similar to DENV from other countries. Conclusions: We revealed the distribution of DENV serotypes and genotypes in Sukabumi. Compared to data obtained from other cities in Indonesia, we observed the differing predominance of DENV serotypes but similar genotype distribution, where the infecting viruses were closely related with Indonesian endemic viruses isolated previously.

531: Quantitative Real-Time RT-PCR for AMACR Distinguishes Prostate Cancer with High Specificity From Benign Lesions

2005 ◽  
Vol 173 (4S) ◽  
pp. 145-145 ◽  
Author(s):  
Martin Schostak ◽  
Hans Krause ◽  
Jens Köllermann ◽  
Mark Schrader ◽  
Bernd Straub ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
High Specificity ◽  
Rt Pcr ◽  
Benign Lesions

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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