scholarly journals Characterization of Avian Influenza Virus H10–H12 Subtypes Isolated from Wild Birds in Shanghai, China from 2016 to 2019

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1085
Author(s):  
Ling Tang ◽  
Wangjun Tang ◽  
Le Ming ◽  
Jianming Gu ◽  
Kai Qian ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
North American ◽  
Large Scale ◽  
Phylogenetic Analyses ◽  
Wild Birds ◽  
The North ◽  
Routine Surveillance ◽  
Eurasian Lineage

H10, H11 and H12 (H10–H12) subtypes of the avian influenza virus (AIV) are associated with waterfowl. Although these subtypes of AIV are infrequently detected in nature, they can undergo reassortment with other AIV subtypes. Few H10–H12 subtypes of AIV have been isolated from wild birds in China. In this study, 12 AIV isolates of H10–H12 subtypes were identified via routine surveillance of wild birds in Shanghai, China from 2016 to 2019, including two H10, three H11 and seven H12 isolates. Sequence and phylogenetic analyses revealed that the genomic segments of the 12 isolates are highly diverse. These 12 isolates are closely related to those in the Eurasian lineage and share a high degree of sequence identity with those from wild birds and domestic ducks in countries in the East Asian–Australasian Flyway, including Japan, Korea, Bangladesh, Vietnam and China. However, parts of the genomic segments of two H12N2 isolates (NH112319-H12N2 and NH101807-H12N2) belong to the North American lineage, suggesting intercontinental reassortment among H12 AIVs in Eurasia and North American. To better understand the ecological and phylodynamic features of H10–H12 subtypes in wild birds, a large-scale surveillance of AIVs in wild birds is warranted.

scholarly journals Detection of a Reassortant H9N2 Avian Influenza Virus with Intercontinental Gene Segments in a Resident Australian Chestnut Teal

Viruses ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 88 ◽  
Author(s):  
Tarka Raj Bhatta ◽  
Anthony Chamings ◽  
Jessy Vibin ◽  
Marcel Klaassen ◽  
Soren Alexandersen
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Phylogenetic Analyses ◽  
Gene Segment ◽  
Wild Birds ◽  
Influenza Viruses ◽  
H9n2 Avian Influenza Virus ◽  
The North ◽  
Southeastern Australia

The present study reports the genetic characterization of a low-pathogenicity H9N2 avian influenza virus, initially from a pool and subsequently from individual faecal samples collected from Chestnut teals (Anas castanea) in southeastern Australia. Phylogenetic analyses of six full gene segments and two partial gene segments obtained from next-generation sequencing showed that this avian influenza virus, A/Chestnut teal/Australia/CT08.18/12952/2018 (H9N2), was a typical, low-pathogenicity, Eurasian aquatic bird lineage H9N2 virus, albeit containing the North American lineage nucleoprotein (NP) gene segment detected previously in Australian wild birds. This is the first report of a H9N2 avian influenza virus in resident wild birds in Australia, and although not in itself a cause of concern, is a clear indication of spillover and likely reassortment of influenza viruses between migratory and resident birds, and an indication that any lineage could potentially be introduced in this way.

scholarly journals Avian Influenza Virus H5 Strain with North American and Eurasian Lineage Genes in an Antarctic Penguin

2016 ◽  
Vol 22 (12) ◽  
pp. 2221-2223 ◽  
Author(s):  
Gonzalo P. Barriga ◽  
Dusan Boric-Bargetto ◽  
Marcelo Cortez San Martin ◽  
Víctor Neira ◽  
Harm van Bakel ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
North American ◽  
Eurasian Lineage

scholarly journals Complete Genome Sequence of an H5N2 Avian Influenza Virus Isolated from a Parrot in Southern China

2012 ◽  
Vol 86 (16) ◽  
pp. 8890-8891 ◽  
Author(s):  
Peirong Jiao ◽  
Liangmeng Wei ◽  
Runyu Yuan ◽  
Lang Gong ◽  
Lan Cao ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Genome Sequence ◽  
North American ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Sequence ◽  
Southern China ◽  
The North

We report the complete genome sequence of an H5N2 avian influenza virus (AIV) that was first isolated from a parrot in Guangdong in southern China in 2004. Genomic sequence and phylogenetic analyses showed that it was highly homologous with the North American H5N2 viruses and all eight genes of this virus belonged to the North American gene lineage. These data will help in the investigation of the epidemiology and host range of AIVs in southern China.

scholarly journals Landscape effects and spatial patterns of avian influenza virus in Danish wild birds, 2006‐2020

2021 ◽  
Author(s):  
Lene Jung Kjær ◽  
Charlotte Kristiane Hjulsager ◽  
Lars Erik Larsen ◽  
Anette Ella Boklund ◽  
Tariq Halasa ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Spatial Patterns ◽  
Wild Birds ◽  
Landscape Effects

scholarly journals Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Yuan Li ◽  
Hongliu Ye ◽  
Meng Liu ◽  
Suquan Song ◽  
Jin Chen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Large Scale ◽  
Operation Time ◽  
Reference Method ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Concentration

Abstract Background H7 subtype avian influenza has caused great concern in the global poultry industry and public health. The conventional serological subtype-specific diagnostics is implemented by hemagglutination inhibition (HI) assay despite lengthy operation time. In this study, an efficient, rapid and high-throughput competitive enzyme-linked immunosorbent assay (cELISA) was developed for detection of antibodies against H7 avian influenza virus (AIV) based on a novel monoclonal antibody specific to the hemagglutinin (HA) protein of H7 AIV. Results The reaction parameters including antigen coating concentration, monoclonal antibody concentration and serum dilution ratio were optimized for H7 antibody detection. The specificity of the cELISA was tested using antisera against H1 ~ H9, H11 ~ H14 AIVs and other avian viruses. The selected cut-off values of inhibition rates for chicken, duck and peacock sera were 30.11, 26.85 and 45.66% by receiver-operating characteristic (ROC) curve analysis, respectively. With HI test as the reference method, the minimum detection limits for chicken, duck and peacock positive serum reached 20, 21 and 2− 1 HI titer, respectively. Compared to HI test, the diagnostic accuracy reached 100, 98.6, and 99.3% for chicken, duck and peacock by testing a total of 400 clinical serum samples, respectively. Conclusions In summary, the cELISA assay developed in this study provided a reliable, specific, sensitive and species-independent serological technique for rapid detection of H7 antibody, which was applicable for large-scale serological surveillance and vaccination efficacy evaluation programs.

Comparison of Cloacal and Oropharyngeal Samples for the Detection of Avian Influenza Virus in Wild Birds

2010 ◽  
Vol 5 (1) ◽  
pp. e22-e23
Author(s):  
Naresh Jindal ◽  
Martha De Abin ◽  
Alexander E. Primus ◽  
Subathra Raju ◽  
Yogesh Chander ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Wild Birds

scholarly journals Characterization of the Whole-Genome Sequence of an H3N6 Avian Influenza Virus, Isolated from a Domestic Duck in Guangxi, Southern China

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Tingting Liu ◽  
Zhixun Xie ◽  
Sisi Luo ◽  
Liji Xie ◽  
Xianwen Deng ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Southern China ◽  
Scientific Evidence ◽  
Whole Genome Sequence ◽  
Domestic Duck ◽  
Guangxi Province ◽  
Eurasian Lineage

A field strain of H3N6 avian influenza virus (AIV), A/duck/Guangxi/175D12/2014(H3N6), was isolated from a native duck in Guangxi Province, southern China, in 2014. All of the eight AIV gene segments were sequenced, and sequence results revealed that there were 11 amino acid deletions at the NA stalk region. The NA, PB2, and NP genes showed highest homology to H5N6 AIV, and the PA gene showed highest homology to H7N2 AIV. Phylogenetic analysis indicated that the eight AIV gene segments belonged to the Eurasian lineage. These findings provide scientific evidence of possible or potential mutations of H3N6 AIV circulating in waterfowl in southern China.

scholarly journals Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1046 ◽  
Author(s):  
Seon-Ju Yeo ◽  
Duc-Duong Than ◽  
Hong-Seog Park ◽  
Haan Woo Sung ◽  
Hyun Park
Keyword(s):  
Influenza Virus ◽  
Amino Acid ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Influenza A ◽  
Matrix Protein ◽  
Sequence Similarity ◽  
Wild Birds ◽  
Structural Protein ◽  
Wild Duck

A novel avian influenza virus (A/wild duck/Korea/K102/2018) (H2N9) was isolated from wild birds in South Korea in 2018, and phylogenetic and molecular analyses were conducted on complete gene sequences obtained by next-generation sequencing. Phylogenetic analysis indicated that the hemagglutinin (HA) and neuraminidase (NA) genes of the A/wild duck/Korea/K102/2018 (H2N9) virus belonged to the Eurasian countries, whereas other internal genes (polymerase basic protein 1 (PB1), PB2, nucleoprotein (NP), polymerase acidic protein (PA), matrix protein (M), and non-structural protein (NS)) belonged to the East Asian countries. A monobasic amino acid (PQIEPR/GLF) at the HA cleavage site, E627 in the PB2 gene, and no deletion of the stalk region in the NA gene indicated that the A/wild duck/Korea/K102/2018 (H2N9) isolate was a typical low pathogenicity avian influenza (LPAI). Nucleotide sequence similarity analysis of HA revealed that the highest homology (98.34%) is to that of A/duck/Mongolia/482/2015 (H2N3), and amino acid sequence of NA was closely related to that of A/duck/Bangladesh/8987/2010 (H10N9) (96.45%). In contrast, internal genes showed homology higher than 98% compared to those of other isolates derived from duck and wild birds of China or Japan in 2016–2018. The newly isolated A/wild duck/Korea/K102/2018 (H2N9) strain is the first reported avian influenza virus in Korea, and may have evolved from multiple genotypes in wild birds and ducks in Mongolia, China, and Japan.

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