scholarly journals Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 578
Author(s):  
Irene Cano ◽  
David Stone ◽  
Jacqueline Savage ◽  
Gareth Wood ◽  
Brian Mulhearn ◽  
...  

This is the first record of a fish nidovirus isolated from a consignment of goldfish at the United Kingdom (UK) border. The full-length viral genome was 25,985 nt, sharing a 97.9% nucleotide identity with the Chinook salmon bafinivirus (CSBV) NIDO with two deletions of 537 and 480 nt on the ORF Ia protein. To assess the potential impact on UK fish species, Atlantic salmon, common carp and goldfish were exposed to the virus via an intraperitoneal (IP) injection and bath challenge. Moribundity was recorded in only 8% of IP-injected goldfish. A high viral load, ≈107 of the CSBV PpIa gene, was measured in the kidney of moribund goldfish. Mild histopathological changes were observed in the kidneys of challenged carps. Ultrastructural observations in renal tubule epithelial cells of goldfish showed cylindrical tubes (≈15 nm in diameter) and tubular structures budding spherical virions (≈200 nm in diameter) with external spike-like structures. Negative staining showed both circular and bacilliform virions. Seroconversion was measured in common carp and goldfish but not in Atlantic salmon. This study reinforces the potential risk of novel and emerging pathogens being introduced to recipient countries via the international ornamental fish trade and the importance of regular full health screens at the border inspection posts to reduce this risk.

1951 ◽  
Vol 5 (4) ◽  
pp. 810-811

A joint meeting of the Research Committee on Agriculture, Fish, Wildlife and Forestry and the Preparatory Fisheries Committee was held in Trinidad on July 9, 1951. The meeting, under the chairmanship of the United Kingdom representative (Frampton), discussed proposals regarding commercial trawl fishing in the Gulf of Paria and the organization of the Fisheries Conference. The committee felt that a survey of commercial trawl fishing should be undertaken at an estimated expense of $2,000. The committee also recommended that the Fisheries Conference, to be held in March 1952 for five days, should meet in Trinidad and have an agenda as follows: occurrence and distribution of commercial fish species in the Caribbean, techniques used in the Caribbean for fish capture, types of fishing craft used, marketing, storing, distribution, conserving methods, and cultivation of fish in ponds.


1992 ◽  
Vol 41 (1) ◽  
pp. 95-97 ◽  
Author(s):  
G. HALL ◽  
S. DOBSON ◽  
C. NICHOLLS

2004 ◽  
Vol 154 (14) ◽  
pp. 437-438 ◽  
Author(s):  
M. T. Fox ◽  
A. S. Baker ◽  
R. G. Farquhar ◽  
E. Eve

2020 ◽  
Vol 42 ◽  
pp. 2 ◽  
Author(s):  
F. Newbery ◽  
R.J. Robinson ◽  
E. Beal ◽  
J. Scrace

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Frithjof Christian Küpper ◽  
Kyle Fletcher ◽  
Ingo Maier

Abstract The finding of the enigmatic pathogen Anisolpidium rosenvingei in the filamentous brown macroalga Pylaiella littoralis presented a unique opportunity to histochemically study the distribution of chitin in this little-known pathogen using FUNGALASE™-F, a fluorescein-labelled chitinase. Chitin was found localised to the exit tube of this pathogen, which infects exclusively reproductive cells of its host. The cytological and phylogenetic implications of this finding are discussed. This paper also reports the first record of this pathogen in the United Kingdom, on the west coast of Scotland.


2012 ◽  
Vol 51 (No. 5) ◽  
pp. 296-302 ◽  
Author(s):  
T. Vesely ◽  
S. Reschova ◽  
D. Pokorova ◽  
J. Hulova ◽  
Z. Nevorankova

A method for purification of carp serum immunoglobulin (IgM), intended for the production of monoclonal antibodies, was described in the present study. Hybridomas that produce antibodies against IgM heavy chain were selected by ELISA method and Western blotting. Ascitic fluids were prepared and tested by the above mentioned methods, and their typing followed. Monoclonal antibody with the highest titre of antibodies against carp immunoglobulin was selected for conjugation with horseradish peroxidase. Specificity of conjugated monoclonal antibody was tested in a panel of various fish species sera. Cross-reactivity was not detected in rainbow trout (Oncorhynchus mykiss) and eleven other fish species. Besides common carp, positive results were also found in goldfish (Carassius auratus) and bighead carp (Aristichthys nobilis), that are members of Cyprinidae family. Among fish other than Cyprinidae, positive results were also detected in sheatfish (Silurus glanis). The sensitivity in common carp was approximately 10 ng/ml.


2010 ◽  
Vol 46 (No. 4) ◽  
pp. 189-191
Author(s):  
J. Beránek ◽  
I. Šafránková

In November 2009 the gall midge species Horidiplosis ficifolii Harris 2003 was detected in the Czech Republic on leaves of an ornamental fig Ficus microcarpa. The insect is native to South East Asia, where it lives on the genus Ficus. No information is currently available on the prevalence of the pest in the countries of its origin. Imported Ficus plants from China into the Netherlands are sometimes heavily infested with the gall midge. In Europe it is also known from Denmark and the United Kingdom. As Horidiplosis ficifolii is a tropical species, no environmental and social damage is expected.


1973 ◽  
Vol 71 (3) ◽  
pp. 481-486 ◽  
Author(s):  
R. W. S. Harvey ◽  
T. H. Price ◽  
Mary L. M. Hall

SUMMARYIsolations of subgenus III salmonellas (arizonas) made in the regional Public Health Laboratory, Cardiff, between 1959 and 1971 are reviewed. The techniques of isolation are listed for the various materials examined. The necessity of using bismuth sulphite agar as a plating medium is stressed, as some strains might appear on brilliant green MacConkey agar as rapid lactose fermenters and be missed. The serotypes isolated in Cardiff are discussed with reference to isolations by other authors. The culture of a subgenus III salmonella from pig faeces is described. It is thought that this is the first record of such an isolation from a pig in the United Kingdom.


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