scholarly journals Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1126 ◽  
Author(s):  
Carine Fortes Aragão ◽  
Valéria Cristina Soares Pinheiro ◽  
Joaquim Pinto Nunes Neto ◽  
Eliana Vieira Pinto da Silva ◽  
Glennda Juscely Galvão Pereira ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Asian American ◽  
Natural Infection ◽  
Rna Extraction ◽  
Hemorrhagic Fever ◽  
Severe Dengue ◽  
Northeast Brazil ◽  
Epidemiological Risk ◽  
Human Infections ◽  
Dengue Type 2 Virus

Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

scholarly journals Dilemma in laboratory diagnosis of Dengue, Chikungunya and Zika viruses

2019 ◽  
Vol 30 (2) ◽  
pp. 83-92 ◽  
Author(s):  
Mushtaque Ahmed ◽  
Nabeela Mahboob ◽  
Kazi Taib Mamun ◽  
Hasina Iqbal
Keyword(s):  
Hemorrhagic Fever ◽  
Laboratory Diagnosis ◽  
Joint Disease ◽  
Severe Dengue ◽  
Laboratory Methods ◽  
Vector Borne Diseases ◽  
Appropriate Treatment ◽  
Clinical Presentations ◽  
Epidemiological Risk ◽  
Vector Borne

Dengue, Chikungunya and Zika are the vector-borne diseases that constitute a potential epidemiological risk due to the recent increase in cases, complications, and severity. The co-circulation of the three diseases is a matter of public health interest due to their transmission by the same vector as well as the increase in the number of cases of severe dengue hemorrhagic fever, post-chikungunya chronic joint disease and microcephaly related to Zika virus. Therefore, it is important to be familiar with the various clinical presentations and laboratory methods to make the differential diagnosis, start appropriate treatment, and prevent the associated complications. Bangladesh J Medicine July 2019; 30(2) : 83-92

scholarly journals SURVEI JENTIK NYAMUK Aedes aegypti DI PERUMAHAN WILAYAH KERJA PUSKESMAS MENTENG KOTA PALANGKA RAYA

2018 ◽  
Vol 3 (1) ◽  
pp. 55-61
Author(s):  
Rinny Ardina ◽  
Nurhalina Nurhalina ◽  
Suratno Suratno ◽  
Dwi Purbayanti ◽  
Fera Sartika ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Community Service ◽  
Community Health Center ◽  
Hemorrhagic Fever ◽  
Severe Dengue ◽  
Morbidity Rate ◽  
Fatality Rate ◽  
Central Kalimantan ◽  
Site Survey ◽  
Water Container

Severe dengue or Dengue Hemorrhagic Fever (DHF) occurrence in Palangka Raya frequently becomes epidemic. The morbidity rate (incidence rate) of DHF cases in Central Kalimantan in 2016 reached 69.1 per 100,000 population with the mortality rate (fatality rate) of 1.4%. The morbidity rate reached 278.4 per 100,000 population in Palangka Raya, the second place after North Barito regency. Based on the Ministry of Health Republic of Indonesia�s Strategic Planning (Renstra), targets for dengue fever in 2016 should be <49 per 100,000 population, Central Kalimantan Province has not achieved the target yet. The Central Kalimantan Provincial Health Office reported the epidemy of DHF in 2017 occurred in the working area of Menteng Community Health Center. The report found a case that all family members of a household suffered from DHF. This community service aimed to educate the community to survey of dengue larvae independently, to develop the community�s knowledge and awareness about the importance of support from larvae monitoring speculator Juru Pemantau Jentik (also briefly known as Jumantik) and to increase community participation in DHF prevention movement in Palangka Raya. The method of this community service used site survey, observation, interview, larva examination and reporting of results. The survey activity of larvae showed that Aedes aegypti mosquito larvae were identified in 13.3% of the houses, mostly found in bathtubs, water shelters and water container of water dispensers.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

scholarly journals Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia

Biomédica ◽  
2017 ◽  
Vol 37 ◽  
pp. 135 ◽  
Author(s):  
Andrés Gómez-Palacio ◽  
Juan Suaza-Vasco ◽  
Sandra Castaño ◽  
Omar Triana ◽  
Sandra Uribe
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Aedes Albopictus ◽  
Distribución Geográfica ◽  
Dengue Type 2 Virus

Introducción. Aedes aegypti y Ae. albopictus son reconocidos vectores de arbovirus como los del dengue, la fiebre amarilla, el chikungunya y el Zika, en regiones tropicales y subtropicales del mundo. En Colombia, la distribución geográfica de Ae. albopictus ha sufrido un incremento y hoy incluye ciudades como Cali y Medellín. Hasta ahora, sin embargo, no se ha recabado información concluyente sobre su infección viral y su capacidad de transmisión a los humanos.Objetivo. Determinar la infección natural por dengue en ejemplares de Ae. albopictus recolectados en un área urbana de Medellín.Materiales y métodos. Se recolectaron individuos de Ae. albopictus en el campus de la Universidad Nacional de Colombia, sede Medellín. Se confirmó su clasificación taxonómica mediante el análisis del gen citocromo oxidasa I (COI), y se extrajo el ARN total para la identificación del virus del dengue y de los respectivos serotipos. La presencia del genotipo DENV se infirió mediante el análisis del gen NS3.Resultados. El análisis del COI corroboró el estatus taxonómico de Ae. albopictus. Uno de los mosquitos procesados fue positivo para DENV-2 y el análisis del NS3 mostró una gran similitud con el genotipo asiático-americano.Conclusión. Se reporta la infección con DENV-2 en Ae. albopictus en Medellín, Colombia. La presencia del genotipo asiático-americano en una zona urbana sugiere su posible circulación entre humanos y en Ae. albopictus, lo cual alerta sobre su eventual papel en la transmisión del DENV-2, y sobre la necesidad de incluir esta especie en la vigilancia entomológica en Colombia.

Transplantation of Adult Dirofilaria roemeri to Grey Kangaroos and Laboratory Rats

1972 ◽  
Vol 46 (1) ◽  
pp. 81-89 ◽  
Author(s):  
David M. Spratt
Keyword(s):  
Aedes Aegypti ◽  
Peritoneal Lavage ◽  
Natural Infection ◽  
Experimental Transmission ◽  
Laboratory Rats ◽  
Saphenous Veins ◽  
Large Numbers ◽  
Grey Kangaroo ◽  
Transmission Studies ◽  
Excellent Source

Adult Dirofilaria roemeri were transplanted subcutaneously into two grey kangaroos and intraperitoneally into seven laboratory rats in an investigation of amicrofilaraemia, and for experimental transmission studies. Low level blood microfilaraemias of only short duration were produced in all but one rat, supporting the hypothesis that the grey kangaroo is an abnormal host of this parasite. Cortisone was instrumental in the success of D. roemeri in rats. Peritoneal lavage of rats harbouring transplanted D. roemeri proved an excellent source of microfilariae. Injection of large numbers of microfilariae into the saphenous veins of rats harbouring transplanted worms failed to alter the number of circulating microfilariae. D. roemeri failed to develop in Aedes aegypti, fed on rats harbouring transplanted worms and exhibiting blood microfilaraemia. One kangaroo, to which worms had been transplanted, was exposed to the bites of Dasybasis hebes (Diptera, Tabanidae) in the study area and acquired a natural infection of D. roemeri.

scholarly journals Two Complex, Adenovirus-Based Vaccines That Together Induce Immune Responses to All Four Dengue Virus Serotypes

2006 ◽  
Vol 14 (2) ◽  
pp. 182-189 ◽  
Author(s):  
David H. Holman ◽  
Danher Wang ◽  
Kanakatte Raviprakash ◽  
Nicholas U. Raja ◽  
Min Luo ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dengue Virus ◽  
Vaccine Development ◽  
De Novo ◽  
Natural Infection ◽  
Hemorrhagic Fever ◽  
Virus Infections ◽  
Dengue Vaccine ◽  
Membrane Antigens ◽  
Dengue Virus Serotypes

ABSTRACT Dengue virus infections can cause hemorrhagic fever, shock, encephalitis, and even death. Worldwide, approximately 2.5 billion people live in dengue-infested regions with about 100 million new cases each year, although many of these infections are believed to be silent. There are four antigenically distinct serotypes of dengue virus; thus, immunity from one serotype will not cross-protect from infection with the other three. The difficulties that hamper vaccine development include requirements of the natural conformation of the envelope glycoprotein to induce neutralizing immune responses and the necessity of presenting antigens of all four serotypes. Currently, the only way to meet these requirements is to use a mixture of four serotypes of live attenuated dengue viruses, but safety remains a major problem. In this study, we have developed the basis for a tetravalent dengue vaccine using a novel complex adenovirus platform that is capable of expressing multiple antigens de novo. This dengue vaccine is constructed as a pair of vectors that each expresses the premembrane and envelope genes of two different dengue virus serotypes. Upon vaccination, the vaccine expressed high levels of the dengue virus antigens in cells to mimic a natural infection and induced both humoral and cellular immune responses against multiple serotypes of dengue virus in an animal model. Further analyses show the humoral responses were indeed neutralizing against all four serotypes. Our studies demonstrate the concept of mimicking infections to induce immune responses by synthesizing dengue virus membrane antigens de novo and the feasibility of developing an effective tetravalent dengue vaccine by vector-mediated expression of glycoproteins of the four serotypes.

scholarly journals Changes in infestation sites of female Aedes aegypti in Northeast Brazil

2016 ◽  
Vol 49 (4) ◽  
pp. 498-501 ◽  
Author(s):  
Luciano Pamplona de Góes Cavalcanti ◽  
Rhaquel de Morais Alves Barbosa Oliveira ◽  
Carlos Henrique Alencar
Keyword(s):  
Aedes Aegypti ◽  
Northeast Brazil

scholarly journals EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

2020 ◽  
Vol 19 (2) ◽  
pp. 112-118
Author(s):  
Muhammad Rasyid Ridha ◽  
Budi Hairani ◽  
Gusti Meliyanie ◽  
Wulan Rasna Giri Sembiring ◽  
Abdullah Fadilly ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Control Strategy ◽  
Oryza Sativa L ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Alternative Control ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water   ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

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