scholarly journals Identification and Phylogenetic Characterization of Human Enteroviruses Isolated from Cases of Aseptic Meningitis in Brazil, 2013–2017

Viruses ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 690 ◽  
Author(s):  
Emanuelle Ramalho ◽  
Ivanildo Sousa ◽  
Fernanda Burlandy ◽  
Eliane Costa ◽  
Amanda Dias ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Neurological Diseases ◽  
Seasonal Pattern ◽  
Temporal Distribution ◽  
Phylogenetic Characterization ◽  
Echovirus 30 ◽  
Sporadic Cases ◽  
Cell Culture Isolation ◽  
Enterovirus Serotypes

Aseptic meningitis is a common viral infection associated with human enteroviruses. The aim of the present study was to identify and characterize the enteroviruses associated with outbreaks and sporadic cases of aseptic meningitis that occurred in different regions of Brazil between 2013 and 2017. Cerebrospinal fluids obtained from patients admitted to public health facilities were analyzed. A total of 303 patients were positive for Human Enteroviruses (EV) by cell culture isolation with a median isolation rate throughout the year of 12%. We were able to identify enterovirus serotypes in 295 clinical specimens. Nineteen different serotypes were identified; the large majority corresponded to HEV-B species. Echovirus 30 (E-30) and Echovirus 6 (E-6) were the most prevalent genotypes (66.8%). Sequence analysis suggested that circulating E-30 was closely related to E-30 from other American countries; while E-6 was derived from Europe. Most of the patients consisted of children ≤ 15 years old. The temporal distribution of all aseptic meningitis and EV-positive cases showed an obvious seasonal pattern during autumn. Our results have provided valuable information about the enteroviral etiology of the aseptic meningitis cases in Brazil pointing to the importance of enterovirus surveillance in neurological diseases.

Aseptic meningitis outbreak caused by echovirus 30 in two regions in Bulgaria, May–August 2012

2013 ◽  
Vol 142 (10) ◽  
pp. 2159-2165 ◽  
Author(s):  
Z. MLADENOVA ◽  
G. BUTTINELLI ◽  
A. DIKOVA ◽  
A. STOYANOVA ◽  
M. TROYANCHEVA ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Index Case ◽  
Serological Test ◽  
Close Correlation ◽  
Amino Acid Identity ◽  
Aetiological Agent ◽  
Echovirus 30 ◽  
First Case ◽  
Sporadic Cases ◽  
Cell Culture Isolation

SUMMARYAn aseptic meningitis outbreak emerged in two regions in Bulgaria in 2012 and echovirus 30 (E30) was established as the aetiological agent by cell culture isolation, serological test, and molecular-based techniques. A total of 157 patients with aseptic meningitis were investigated, of which 117 were confirmed as having E30-associated disease. Molecular analysis of 12 E30 isolates revealed 99–100% nucleotide and amino-acid identity between them and a close correlation with a Greek strain involved in an E30 outbreak in 2012. Children aged 5–14 years were mainly affected, which could reflect the absence of E30 epidemics in Bulgaria for a period of 11 years. The first case with E30 isolation (a 2-year-old patient from Plovdiv) was notified at the end of April 2012. This was most likely the index case, from which the spread of the virus started, causing sporadic cases first, which later led to an aseptic meningitis outbreak facilitated by person-to-person viral transmission.

scholarly journals Genetic characterization of a novel recombinant echovirus 30 strain causing a regional epidemic of aseptic meningitis in Hokkaido, Japan, 2017

2019 ◽  
Vol 165 (2) ◽  
pp. 433-438
Author(s):  
Masahiro Miyoshi ◽  
Akiko Goto ◽  
Rika Komagome ◽  
Hiroki Yamaguchi ◽  
Yuji Maruo ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Genetic Characterization ◽  
Echovirus 30

scholarly journals Molecular Characterization of Echovirus 30 Isolated from Environment in North India

2020 ◽  
Author(s):  
Sarika Tiwari ◽  
Tapan N Dhole
Keyword(s):  
Aseptic Meningitis ◽  
The United States ◽  
North India ◽  
Rt Pcr ◽  
Specific Primers ◽  
Virus Stock ◽  
Echovirus 30 ◽  
Rd Cells ◽  
Infected Cells

Abstract BackgroundEchovirus 30 (E30) causes acute aseptic meningitis. Enteroviruses (EVs) are responsible for 30,000 to 50,000 hospitalizations for aseptic meningitis per year in the United States. E30 is one of the most frequently isolated EVs, causing extensive outbreaks in temperate climates in several countries. MethodsE30 used in this study was isolated from environmental specimens. The virus was confirmed by RT-PCR with specific primers (1). Virus stock was prepared by infecting RD cells in 25 cm2 flasks (Corning Inc. USA). Virus infected and mock infected cells were incubated at 36.5 ºC with 2% MEM. After 48 h of infection, the culture was aliquoted and kept at-80 ºC for further use.ResultsThis present work analyzed the E30 genetic diversity in a fragment of 217 nucleotides of the VP1 region, of environmental strains. The environmental samples of echovirus 30 show mutation in the nonfunctional polyprotein protein (Fig. 1). The result suggested that the information may be obtained by analyzing only a part of the VP1 region.ConclusionThe present study showed that the E30 environmental sample is more divergent to prototype Bastianni strain.

scholarly journals Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012

2013 ◽  
Vol 10 (1) ◽  
pp. 263 ◽  
Author(s):  
Hong Xiao ◽  
Dawei Guan ◽  
Ruiyu Chen ◽  
Pingxi Chen ◽  
Corina Monagin ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Aseptic Meningitis ◽  
Echovirus 30

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization

Sequencing and phylogenetic characterization of a novel RNA virus in Arma chinensis

2021 ◽  
Vol 65 (03) ◽  
pp. 320-323
Author(s):  
Yonghao Dong ◽  
Pengjun Xu ◽  
Guangwei Ren ◽  
Changchun Feng ◽  
Dongyang Liu ◽  
...  
Keyword(s):  
Rna Virus ◽  
Phylogenetic Characterization
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