scholarly journals Cell Culture Models for Hepatitis E Virus

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 608 ◽  
Author(s):  
Rebecca Menhua Fu ◽  
Charlotte Caroline Decker ◽  
Viet Loan Dao Thi
Keyword(s):  
Cell Culture ◽  
Life Cycle ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Cellular Systems ◽  
Advantages And Disadvantages ◽  
Infectious Clones ◽  
Cell Culture Systems ◽  
Culture Models ◽  
Cell Culture Models

Despite a growing awareness, hepatitis E virus (HEV) remains understudied and investigations have been historically hampered by the absence of efficient cell culture systems. As a result, the pathogenesis of HEV infection and basic steps of the HEV life cycle are poorly understood. Major efforts have recently been made through the development of HEV infectious clones and cellular systems that significantly advanced HEV research. Here, we summarize these systems, discussing their advantages and disadvantages for HEV studies. We further capitalize on the need for HEV-permissive polarized cell models to better recapitulate the entire HEV life cycle and transmission.

scholarly journals Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1

2021 ◽  
Author(s):  
Yunlong Li ◽  
Zhijiang Miao ◽  
Pengfei Li ◽  
Ruyi Zhang ◽  
Denis E. Kainov ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Nuclear Transport ◽  
Hepatitis E ◽  
Term Treatment ◽  
Cellular Target ◽  
Long Term Treatment ◽  
Culture Models ◽  
Transport Complex ◽  
Cell Culture Models

AbstractWe show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance. Gene silencing of importin-α1, a cellular target of ivermectin and a key member of the host nuclear transport complex, inhibited viral replication and largely abolished the anti-HEV effect of ivermectin.

Cell culture studies: a promising approach to metabolomic study of human aging

2021 ◽  
Vol 08 ◽  
Author(s):  
Ana Rocha ◽  
Sandra Magalhães ◽  
Alexandra Nunes
Keyword(s):  
Cell Culture ◽  
Computational Models ◽  
Culture Studies ◽  
Advantages And Disadvantages ◽  
Morphologic Characteristics ◽  
Aging Studies ◽  
Culture Models ◽  
Biological Heterogeneity ◽  
Cell Culture Models ◽  
Metabolomic Study

: With the increasing aging of the world’s population, a detailed study of the characteristics of aging, as well as the pathologies this process can lead to, is crucial. Therefore, there are several study models for the study of aging, from computational models to animals or even to cell cultures. The latter have shown high potential for aging studies as they are easier to handle, cheaper, do not require the same level of ethical consideration required for animal and human studies and present little biological heterogeneity when grown under the same conditions and in the same context population. For aging studies, these characteristics are a great advantage since cells have a huge variety of morphologic characteristics and markers that can be studies. Thus, the aim of this review is to offer an overview of the models used in studies of aging, with focus on cell culture models, presenting the advantages and disadvantages of cell culture in the study of aging, of what information can we extract of these studies and how cell studies can be compared with the other models.

Natural Life Cycle of Arthropod-Borne Togaviruses: Inferences from Cell Culture Models

1978 ◽  
pp. 281-298 ◽  
Author(s):  
R. WALTER SCHLESINGER ◽  
VICTOR STOLLAR ◽  
AKIRA IGARASHI ◽  
GREGORY M. GUILD ◽  
GRAHAM R. CLEAVES
Keyword(s):  
Cell Culture ◽  
Life Cycle ◽  
Culture Models ◽  
Cell Culture Models ◽  
Natural Life

scholarly journals Animal Models for Hepatitis E Virus

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 564 ◽  
Author(s):  
Laura Corneillie ◽  
Dominic Banda ◽  
Philip Meuleman
Keyword(s):  
Animal Models ◽  
Hepatitis E Virus ◽  
Acute Viral Hepatitis ◽  
Hepatitis E ◽  
Medical Problem ◽  
Western World ◽  
Species Barrier ◽  
The Past ◽  
Culture Models ◽  
Cell Culture Models

Hepatitis E virus (HEV) is an underdiagnosed pathogen with approximately 20 million infections each year and currently the most common cause of acute viral hepatitis. HEV was long considered to be confined to developing countries but there is increasing evidence that it is also a medical problem in the Western world. HEV that infects humans belongs to the Orthohepevirus A species of the Hepeviridae family. Novel HEV-like viruses have been observed in a variety of animals and some have been shown to be able to cross the species barrier, causing infection in humans. Several cell culture models for HEV have been established in the past years, but their efficiency is usually relatively low. With the circulation of this virus and related viruses in a variety of species, several different animal models have been developed. In this review, we give an overview of these animal models, indicate their main characteristics, and highlight how they may contribute to our understanding of the basic aspects of the viral life cycle and cross-species infection, the study of pathogenesis, and the evaluation of novel preventative and therapeutic strategies.

scholarly journals Cell culture systems for the study of hepatitis E virus

2019 ◽  
Vol 163 ◽  
pp. 34-49 ◽  
Author(s):  
Toni L. Meister ◽  
Janina Bruening ◽  
Daniel Todt ◽  
Eike Steinmann
Keyword(s):  
Cell Culture ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Culture Systems ◽  
Cell Culture Systems
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