scholarly journals Surveillance for Adenoviruses in Bats in Italy

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 523 ◽  
Author(s):  
Georgia Diakoudi ◽  
Gianvito Lanave ◽  
Ana Moreno ◽  
Chiara Chiapponi ◽  
Enrica Sozzi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Genome Organization ◽  
Northern Italy ◽  
Polymerase Gene ◽  
Sequencing Technologies ◽  
Diagnostic Fragment ◽  
Gathering Data ◽  
Simian Cell

Adenoviruses are important pathogens of humans and animals. Bats have been recognized as potential reservoirs of novel viruses, with some viruses being regarded as a possible zoonotic threat to humans. In this study, we report the detection and analysis of adenoviruses from different bat species in northern Italy. Upon sequence and phylogenetic analysis, based on a short diagnostic fragment of the highly-conserved DNA polymerase gene, we identified potential novel candidate adenovirus species, including an avian-like adenovirus strain. An adenovirus isolate was obtained in simian cell lines from the carcass of a Pipistrellus kuhlii, and the complete genome sequence was reconstructed using deep sequencing technologies. The virus displayed high nucleotide identity and virtually the same genome organization as the Pipistrellus pipistrellus strain PPV1, isolated in Germany in 2007. Gathering data on epidemiology and the genetic diversity of bat adenoviruses may be helpful to better understand their evolution in the mammalian and avian hosts.

scholarly journals Phylogenetic Analysis Indicates Evolutionary Diversity and Environmental Segregation of Marine Podovirus DNA Polymerase Gene Sequences

2009 ◽  
Vol 75 (11) ◽  
pp. 3634-3640 ◽  
Author(s):  
Jessica M. Labonté ◽  
Karen E. Reid ◽  
Curtis A. Suttle
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Pcr Primers ◽  
Geographic Region ◽  
Polymorphism Analysis ◽  
Polymerase Gene ◽  
Gene Sequences ◽  
High Genetic Diversity ◽  
Evolutionary Relatedness

ABSTRACT The distribution of viral genotypes in the ocean and their evolutionary relatedness remain poorly constrained. This paper presents data on the genetic diversity and evolutionary relationships of 1.2-kb DNA polymerase (pol) gene fragments from podoviruses. A newly designed set of PCR primers was used to amplify DNA directly from coastal sediment and water samples collected from inlets adjacent to the Strait of Georgia, British Columbia, Canada, and from the northeastern Gulf of Mexico. Restriction fragment length polymorphism analysis of 160 cloned PCR products revealed 29 distinct operational taxonomic units (OTUs), with OTUs within a site typically being more similar than those among sites. Phylogenetic analysis of the DNA pol gene fragments demonstrated high similarity between some environmental sequences and sequences from the marine podoviruses roseophage SIO1 and cyanophage P60, while others were not closely related to sequences from cultured phages. Interrogation of the CAMERA database for sequences from metagenomics data demonstrated that the amplified sequences were representative of the diversity of podovirus pol sequences found in marine samples. Our results indicate high genetic diversity within marine podovirus communities within a small geographic region and demonstrate that the diversity of environmental polymerase gene sequences for podoviruses is far more extensive than previously recognized.

scholarly journals Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis globosa (Prymnesiophyceae)

2004 ◽  
Vol 70 (6) ◽  
pp. 3700-3705 ◽  
Author(s):  
C. P. D. Brussaard ◽  
S. M. Short ◽  
C. M. Frederickson ◽  
C. A. Suttle
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Coastal Waters ◽  
Common Ancestor ◽  
Genetic Relatedness ◽  
Amino Acid Sequences ◽  
Polymerase Gene ◽  
Phaeocystis Globosa ◽  
The Family ◽  
Algal Host

ABSTRACT Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved.

Group II Nucleopolyhedrovirus Subgroups Revealed by Phylogenetic Analysis of Polyhedrin and DNA Polymerase Gene Sequences

1999 ◽  
Vol 73 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Dieter M. Bulach ◽  
C.Ananth Kumar ◽  
Angelo Zaia ◽  
Bufeng Liang ◽  
David E. Tribe
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Polymerase Gene ◽  
Gene Sequences ◽  
Dna Polymerase Gene ◽  
Group Ii

scholarly journals First Molecular Evidence for the Existence of Distinct Fish and Snake Adenoviruses

2002 ◽  
Vol 76 (19) ◽  
pp. 10056-10059 ◽  
Author(s):  
Mária Benkő ◽  
Péter Élő ◽  
Krisztina Ursu ◽  
Winfried Ahne ◽  
Scott E. LaPatra ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
New Genus ◽  
Molecular Evidence ◽  
Polymerase Gene ◽  
Snake Species ◽  
Dna Polymerase Gene

ABSTRACT From adenovirus-like viruses originating from a fish and a snake species, a conserved part of the adenoviral DNA polymerase gene was PCR amplified, cloned and sequenced. Phylogenetic analysis showed that the snake adenovirus is closely related to the members of the proposed genus Atadenovirus, whereas the fish isolate seems to represent a separate cluster, likely a new genus.

Phylogenetic Analysis of the DNA Polymerase Gene of a Novel Alphaherpesvirus Isolated from an Indian Gyps Vulture

Virus Genes ◽  
2005 ◽  
Vol 30 (3) ◽  
pp. 371-381 ◽  
Author(s):  
Maria Cardoso ◽  
Alex Hyatt ◽  
Paul Selleck ◽  
Sue Lowther ◽  
Vibhu Prakash ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Polymerase Gene ◽  
Dna Polymerase Gene

Phylogenetic analysis of PgV-102P, a new virus from the English Channel that infects Phaeocystis globosa

2006 ◽  
Vol 86 (3) ◽  
pp. 485-490 ◽  
Author(s):  
William H. Wilson ◽  
Declan C. Schroeder ◽  
Jenna Ho ◽  
Martin Canty
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Harmful Algal Bloom ◽  
English Channel ◽  
Polymerase Gene ◽  
Phaeocystis Globosa ◽  
Dna Viruses ◽  
Algal Virus ◽  
Double Stranded Dna ◽  
Dna Polymerase Gene

A new virus that infects the harmful algal bloom-forming microalga Phaeocystis globosa was isolated from surface water in the English Channel off the coast of Plymouth, UK, in May 2001. Phylogenetic analysis of the DNA polymerase gene revealed the virus isolate, designated PgV-102P, belongs to the family Phycodnaviridae, a group of large double-stranded DNA viruses known to infect algae. Basic characterization of PgV-102P revealed it was a lytic virus with a relatively slow culture lysis period of 10-days. The genome size (176 kbp) and capsid diameter (98 nm) of PgV-102P fall at the bottom end of the range expected for phycodnaviruses. Interestingly, PgV-102P did not cluster with other P. globosa viruses; instead, it was more closely related to other prymnesioviruses that infect the marine prymnesiophyte Chrysochromulina brevifilum. We discuss the effectiveness of DNA polymerase as a diagnostic marker. Although it is ideal for determining what family or even genus an algal virus belongs to, it is clear that the DNA polymerase gene does not have sufficient resolution when looking for relationships within algal virus genera.

scholarly journals Sequence, Function, and Phylogenetic Analysis of an Ascovirus DNA Polymerase Gene

Virology ◽  
1996 ◽  
Vol 216 (1) ◽  
pp. 146-157 ◽  
Author(s):  
BRETT J. PELLOCK ◽  
ALBERT LU ◽  
RICHARD B. MEAGHER ◽  
MICHAEL J. WEISE ◽  
LOIS K. MILLER
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Polymerase Gene ◽  
Dna Polymerase Gene
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