scholarly journals Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 335 ◽  
Author(s):  
Isah Aliyu ◽  
King-Hwa Ling ◽  
Nur Md Hashim ◽  
Jia-Yong Lam ◽  
Hui-Yee Chee
Keyword(s):  
Dengue Virus ◽  
Vero Cells ◽  
Annexin Ii ◽  
Interacting Protein ◽  
Protein Binding Assay ◽  
Successful Infection ◽  
Virus Serotype ◽  
Virus Interaction ◽  
Dengue Virus Serotype 2 ◽  
Denv2 Infection

Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells.

scholarly journals Comparative Evaluation of Permissiveness to Dengue Virus Serotype 2 Infection in Primary Rodent Macrophages

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Jeanette Prada-Arismendy ◽  
Verónica Rincón ◽  
Jaime E. Castellanos
Keyword(s):  
Dengue Virus ◽  
Comparative Evaluation ◽  
Molecular Mechanisms ◽  
Rodent Species ◽  
Oligoadenylate Synthetase ◽  
Virus Serotype ◽  
Mouse Cells ◽  
Dengue Virus Serotype 2 ◽  
Denv2 Infection

Infection with dengue virus presents a broad clinical spectrum, which can range from asymptomatic cases to severe cases that are characterised by haemorrhagic syndrome and/or shock. The reason for such variability remains unknown. This work evaluated thein vitropermissiveness of mouse, rat, hamster and guinea pig macrophages to infection by dengue virus 2 (DENV2). The results established that macrophages derived from the BALB/c mouse strain showed higher permissiveness to DENV2 infection than macrophages from other rodent species, although all rodent species studied had the C820T mutation in the oligoadenylate synthetase 1b gene, indicating no relationship to the differentin vitrosusceptibilities of mouse cells at this locus. Other molecular mechanisms related to flavivirus susceptibility remain to be explored.

Dengue Virus Serotype-2 Interferes with the Formation of Neutrophil Extracellular Traps

Intervirology ◽  
2015 ◽  
Vol 58 (4) ◽  
pp. 250-259 ◽  
Author(s):  
Maria Maximina B. Moreno-Altamirano ◽  
Oscar Rodríguez-Espinosa ◽  
Oscar Rojas-Espinosa ◽  
Bernardo Pliego-Rivero ◽  
Francisco J. Sánchez-García
Keyword(s):  
Dengue Virus ◽  
Neutrophil Extracellular Traps ◽  
Extracellular Traps ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

Impact of Dengue Virus Serotype 2 Strain Diversity on Serological Immune Responses to Dengue

2018 ◽  
Vol 4 (12) ◽  
pp. 1705-1717 ◽  
Author(s):  
Sarah L. Keasey ◽  
Jessica L. Smith ◽  
Stefan Fernandez ◽  
Anna P. Durbin ◽  
Bryan M. Zhao ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dengue Virus ◽  
Strain Diversity ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material

2020 ◽  
Vol 4 (2) ◽  
pp. 40
Author(s):  
Deya Karsari
Keyword(s):  
Amino Acid ◽  
Dengue Virus ◽  
Genetic Stability ◽  
Rna Extraction ◽  
Vero Cells ◽  
Gene Encoding ◽  
Variable Site ◽  
Virus Serotype ◽  
Protein Encoding ◽  
Encoding Protein

This study aims to  analyze   genetic stability of  the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged  in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.

scholarly journals Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line

2019 ◽  
Vol 13 (1) ◽  
pp. 36-42 ◽  
Author(s):  
FITHRIYAH SJATHA ◽  
◽  
OKTIVIA CHANDRA MUSTIKA ◽  
ANGKY BUDIANTI ◽  
TJAHJANI MIRAWATI SUDIRO ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells

2017 ◽  
Vol 50 (1) ◽  
pp. 99-103
Author(s):  
Chye Sheng Gan ◽  
Pei Jean Lim ◽  
Muhammad Fazril Mohamad Razif ◽  
Rohana Yusof ◽  
Shatrah Othman
Keyword(s):  
Dengue Virus ◽  
Hepg2 Cells ◽  
Virus Serotype ◽  
Subunit Expression ◽  
Dengue Virus Serotype 2

Peptides P4 and P7 derived from E protein inhibit entry of dengue virus serotype 2 via interacting with β3 integrin

2018 ◽  
Vol 155 ◽  
pp. 20-27 ◽  
Author(s):  
Xiaoyun Cui ◽  
Yanhua Wu ◽  
Dongying Fan ◽  
Na Gao ◽  
Ying Ming ◽  
...  
Keyword(s):  
Dengue Virus ◽  
E Protein ◽  
Virus Serotype ◽  
Β3 Integrin ◽  
Dengue Virus Serotype 2

Co-existence of major and minor viral populations from two different origins in patients secondarily infected with dengue virus serotype 2 in Bangkok

2011 ◽  
Vol 413 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Orapim Puiprom ◽  
Akifumi Yamashita ◽  
Mikiko Sasayama ◽  
Kriengsak Limkittikul ◽  
Khwanchit Boonha ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Serotype ◽  
Different Origins ◽  
Dengue Virus Serotype 2
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