scholarly journals The Coat Protein of Citrus Yellow Vein Clearing Virus Interacts with Viral Movement Proteins and Serves as an RNA Silencing Suppressor

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 329 ◽  
Author(s):  
Atta Ur Rehman ◽  
Zhuoran Li ◽  
Zuokun Yang ◽  
Muhammad Waqas ◽  
Guoping Wang ◽  
...  
Keyword(s):  
Coat Protein ◽  
Rna Silencing ◽  
Triple Gene Block ◽  
Plant Viruses ◽  
Epidermal Cells ◽  
Yellow Vein ◽  
Silencing Suppressor ◽  
Vein Clearing ◽  
Rna Silencing Suppressor ◽  
Viral Movement

Citrus yellow vein clearing virus is a newly accepted member of the genus Mandarivirus in the family Alphaflexiviridae. The triple gene block proteins (TGBp1, TGBp2 and TGBp3) encoded by plant viruses in this family function on facilitating virus movement. However, the protein function of citrus yellow vein clearing virus (CYVCV) have never been explored. Here, we showed in both yeast two-hybrid (Y2H) and bimolecular fluorescence (BiFC) assays that the coat protein (CP), TGBp1 and TGBp2 of CYVCV are self-interacting. Its CP also interacts with all three TGB proteins, and TGBp1 and TGBp2 interact with each other but not with TGBp3. Furthermore, the viral CP colocalizes with TGBp1 and TGBp3 at the plasmodesmata (PD) of epidermal cells of Nicotiana benthamiana leaves, and TGBp1 can translocate TGBp2 from granular-like structures embedded within ER networks to the PD. The results suggest that these proteins could coexist at the PD of epidermal cells of N. benthamiana. Using Agrobacterium infiltration-mediated RNA silencing assays, we show that CYVCV CP is a strong RNA silencing suppressor (RSS) triggered by positive-sense green fluorescent protein (GFP) RNA. The presented results provide insights for further revealing the mechanism of the viral movement and suppression of RNA silencing.

scholarly journals Rapid screening of RNA silencing suppressors by using a recombinant virus derived from beet necrotic yellow vein virus

2009 ◽  
Vol 90 (10) ◽  
pp. 2536-2541 ◽  
Author(s):  
H. Guilley ◽  
D. Bortolamiol ◽  
G. Jonard ◽  
S. Bouzoubaa ◽  
V. Ziegler-Graff
Keyword(s):  
Rna Silencing ◽  
Plant Defence ◽  
Plant Viruses ◽  
Yellow Vein ◽  
Rapid Screening ◽  
Silencing Suppressor ◽  
Defence Mechanisms ◽  
Simple Method ◽  
Yellow Dwarf Virus ◽  
Plant Defence Mechanisms

To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: rep3–P30, which expresses the movement protein P30 of tobacco mosaic virus, and rep5–X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSSs. Two potential candidates have been tested and we show that, in our system, the P13 protein of burdock mottle virus displays RSS activity while the P0 protein of cereal yellow dwarf virus-RPV does not.

scholarly journals Deciphering the RNA Silencing Suppressor Function in the Potyvirus SPV2

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 26
Author(s):  
Ornela Chase ◽  
Giannina Bambaren ◽  
Juan José López-Moya
Keyword(s):  
Rna Silencing ◽  
Ipomoea Batatas ◽  
Control Strategies ◽  
Regulation Of Gene Expression ◽  
Plant Viruses ◽  
High Yield ◽  
Gene Products ◽  
Silencing Suppressor ◽  
Sweet Potatoes ◽  
Rna Silencing Suppressor

In most eukaryotes, RNA silencing is a key element in the regulation of gene expression and defense against pathogens. Plants have developed a defensive barrier against exogenous microorganisms, such as plant-infecting viruses, by specifically targeting and degrading the viral RNAs and thus limiting the negative effects of the diseases caused by them. On the other hand, plant viruses encode for suppressor proteins that repress the host-silencing machinery, hence allowing viral replication and infection establishment. Our current project focuses on the characterization of gene products contributing to the RNA silencing suppressor (RSS) function of Sweet potato virus 2 (SPV2), genus Potyvirus, family Potyviridae. SPV2 infects sweet potatoes (Ipomoea batatas, family Convolvulaceae), one of the most important staple food crops worldwide. Infections by potyvirids result in the high yield losses of sweet potatoes, especially from coinfection with unrelated viruses, and our final goal is to develop efficient control strategies. Our preliminary results analyzing the P1 and HCPro proteases of SPV2, transiently expressed in N. benthamiana together with a reporter GFP construct, revealed that HCPro constitutes a strong RSS. This is a novel finding, and we are currently characterizing the functions of other gene products.

scholarly journals Identification of the Potential Virulence Factors and RNA Silencing Suppressors of Mulberry Mosaic Dwarf-Associated Geminivirus

Viruses ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 472 ◽  
Author(s):  
Xiuling Yang ◽  
Yanxiang Ren ◽  
Shaoshuang Sun ◽  
Dongxue Wang ◽  
Fanfan Zhang ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Rna Silencing ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Defense Responses ◽  
Plant Viruses ◽  
Silencing Suppressor ◽  
Long Distance ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor

Plant viruses encode virulence factors or RNA silencing suppressors to reprogram plant cellular processes or to fine-tune host RNA silencing-mediated defense responses. In a previous study, Mulberry mosaic dwarf-associated virus (MMDaV), a novel, highly divergent geminivirus, has been identified from a Chinese mulberry tree showing mosaic and dwarfing symptoms, but the functions of its encoded proteins are unknown. In this study, all seven proteins encoded by MMDaV were screened for potential virulence and RNA silencing suppressor activities. We found that V2, RepA, and Rep affect the pathogenicity of a heterologous potato virus X. We showed that V2 could inhibit local RNA silencing and long-distance movement of the RNA silencing signal, but not short-range spread of the green fluorescent protein (GFP) silencing signal in Nicotiana benthamiana 16c plants. In addition, V2 localized to both subnuclear foci and the cytoplasm. Deletion mutagenesis of V2 showed that the basic motif from amino acids 61 to 76 was crucial for V2 to form subnuclear foci and for suppression of RNA silencing. Although the V2 protein encoded by begomoviruses or a curtovirus has been shown to have silencing suppressor activity, this is the first identification of an RNA silencing suppressor from a woody plant-infecting geminivirus.

The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor

2013 ◽  
Vol 177 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Liying Sun ◽  
Ida Bagus Andika ◽  
Jiangfeng Shen ◽  
Di Yang ◽  
Claudio Ratti ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor ◽  
Chinese Wheat

scholarly journals RNA silencing-suppressor function of Turnip crinkle virus coat protein cannot be attributed to its interaction with the Arabidopsis protein TIP

2004 ◽  
Vol 85 (11) ◽  
pp. 3415-3420 ◽  
Author(s):  
Chang Won Choi ◽  
Feng Qu ◽  
Tao Ren ◽  
Xiaohong Ye ◽  
T. Jack Morris
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Amino Acid Substitution ◽  
Rna Silencing ◽  
Single Amino Acid Substitution ◽  
Single Amino Acid ◽  
Silencing Suppressor ◽  
Turnip Crinkle Virus ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor

The interaction of the coat protein (CP) of Turnip crinkle virus (TCV) with a host protein, TCV-interacting protein (TIP), from Arabidopsis thaliana has been reported previously. This interaction correlates with the ability of TCV CP to elicit the resistance response that is mediated by the resistance gene HRT in Arabidopsis ecotype Di-17. It has also been established that TCV CP is a suppressor of RNA silencing, a process by which the host plant targets viral RNA for degradation. These results have led to the speculation that TIP might be a component of the RNA-silencing pathway and that TCV CP suppresses RNA silencing through its interaction with TIP. In the current report, a number of TCV CP mutants have been investigated for their ability to suppress RNA silencing. These mutants include single amino acid substitution mutants that are known to have lost their ability to interact with TIP, as well as deletion mutants of TCV CP that are of different sizes and from different regions of the protein. Results showed that each of the single amino acid substitution mutants tested retained high levels of RNA silencing-suppressor activity. In addition, a mutant containing a 5 aa deletion in the region that is known to be critical for TIP interaction retained the ability to suppress RNA silencing significantly. Larger deletions in all regions of TCV CP abolished silencing-suppressor activity. It can be concluded from these results that the RNA silencing-suppressor activity of TCV CP cannot be attributed to its ability to interact directly with TIP.

RNA Silencing Suppressor p19 Regulates The Expressions of Cell Cycle Related Genes*

2009 ◽  
Vol 36 (5) ◽  
pp. 541-548 ◽  
Author(s):  
Li LIU ◽  
Jian LI ◽  
Yu-Ping XU ◽  
Wen-Tao QIAO ◽  
Qi-Min CHEN ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor

Identification of RNA silencing suppressor encoded by wheat blue dwarf (WBD) phytoplasma

Plant Biology ◽  
2021 ◽  
Author(s):  
Licheng Wang ◽  
Wenbao Chen ◽  
Huan Ma ◽  
Jingyuan Li ◽  
Xingan Hao ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor
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