scholarly journals The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 257
Author(s):  
E Meng ◽  
Baozhen Tang ◽  
Francisco Sanchez-Garcia ◽  
Ting Qiao ◽  
Lang Fu ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Amino Acid Sequence Identity ◽  
Rna Virus ◽  
Complete Sequence ◽  
Matrix Proteins ◽  
Sequence Identity ◽  
The Family ◽  
Family Rhabdoviridae

The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids.

scholarly journals Complete Genome Sequence of a Fusarium graminearum Double-Stranded RNA Virus in a Newly Proposed Family, Alternaviridae

2018 ◽  
Vol 6 (8) ◽  
Author(s):  
Hao He ◽  
Xiaoguang Chen ◽  
Pengfei Li ◽  
Dewen Qiu ◽  
Lihua Guo
Keyword(s):  
Phylogenetic Analysis ◽  
Fusarium Graminearum ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rna Virus ◽  
Phytopathogenic Fungus ◽  
Double Stranded Rna ◽  
First Report ◽  
The Family

ABSTRACT We describe here a double-stranded RNA mycovirus, termed Fusarium graminearum alternavirus 1 (FgAV1/AH11), from the isolate AH11 of the phytopathogenic fungus F. graminearum . Phylogenetic analysis showed that FgAV1/AH11 belongs to a newly proposed family, Alternaviridae . This is the first report of a mycovirus in the family Alternaviridae that infects F. graminearum .

Characterization of a Novel Tombusviridae Species Isolated From Paris polyphylla var. yunnanensis

2021 ◽  
Author(s):  
Mingfu Zhao ◽  
Lu Chen ◽  
Rex Frimpong Anane ◽  
Zhe Wang ◽  
Zeli Chen ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sequence Alignments ◽  
Sequence Comparisons ◽  
Sequence Identity ◽  
Ssrna Virus ◽  
A Genome ◽  
Paris Polyphylla ◽  
The Family

Abstract A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The first ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (p40, nt: 20-1096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2683, p100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic fleck-associated tombusvirus (GCFaV-1,Accession No.QKE30557) had the highest sequence identity (55.5%) with ParV2, and also shares 59.2% RdRp and 34.9% CP amino acid sequence identity with GCFaV-1.Sequence comparisons and phylogenetic analysis of RdRp suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.

scholarly journals Complete Genome Sequence of Seneca Valley Virus CH-01-2015 Identified in China

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Qiwen Wu ◽  
Xiaoya Zhao ◽  
Yanshan Chen ◽  
Xiaoming He ◽  
Guanqun Zhang ◽  
...  
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rna Virus ◽  
The United States ◽  
Chinese Isolate ◽  
Sequence Identity ◽  
Seneca Valley Virus ◽  
Vesicular Disease

The complete genome sequence of Seneca Valley virus (SVV), a single-stranded RNA virus that causes porcine vesicular disease in China, has been sequenced and analyzed. This Chinese isolate shares 94.4 to 97.1% sequence identity to another 8 strains from Canada, Brazil, and the United States. This is the first report of SVV infecting swine in China.

scholarly journals Complete genome sequence of a novel mitovirus isolated from Paris polyphylla var. yunnanensis

2021 ◽  
Author(s):  
Zeli Chen ◽  
Lu Chen ◽  
Rex Frimpong Anane ◽  
Zhe Wang ◽  
Like Gao ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rna Virus ◽  
Genome Structure ◽  
Complementary Sequence ◽  
Reading Frame ◽  
A Genome ◽  
Paris Polyphylla ◽  
The Family

Abstract Paris mitovirus 1 (ParMV1) is a positive-sense RNA virus isolated from diseased Paris polyphylla var. yunnanensis plants in Wenshan, Yunnan. The complete genome sequence of ParMV1 consists of 2,751 nucleotides with a genome structure typical of the mitoviruses. ParMV1 genome has a single open reading frame (ORF: 358-2,637 nt) that encodes RNA-dependent RNA polymerase (RdRp) with a molecular mass of 86.42 kDa. ParMV1 contains six conserved motifs (Ι-VΙ) that are unique to mitoviruses. In addition, the 5′and 3′ terminals of the genome have a stable secondary structure, and the reverse complementary sequence forms a panhandle structure. Comparative genome analysis revealed that ParMV1 shares 23.1–40.6% amino acid (aa) and 32.3–45.7% nucleotide (nt) sequence identities with the RdRp of other mitoviruses. The phylogenetic tree inferred from RdRp aa sequence showed that ParMV1 clusters with mitoviruses, and hence should be considered as a new member of the genus Mitovirus in the family Motiviridae. This is the first report of a novel mitovirus infecting Paris polyphylla var. yunnanensis.

Complete genome sequence and evolution analysis of Eimeria stiedai RNA virus 1, a novel member of the family Totiviridae

2016 ◽  
Vol 161 (12) ◽  
pp. 3571-3576 ◽  
Author(s):  
Caiyan Xin ◽  
Bin Wu ◽  
Jianhua Li ◽  
Pengtao Gong ◽  
Ju Yang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rna Virus ◽  
Evolution Analysis ◽  
The Family ◽  
Eimeria Stiedai

scholarly journals Complete Genome Sequence of a Novel Hypovirus from the Phytopathogenic Fungus Fusarium langsethiae

2017 ◽  
Vol 5 (9) ◽  
Author(s):  
Pengfei Li ◽  
Xiaoguang Chen ◽  
Hao He ◽  
Dewen Qiu ◽  
Lihua Guo
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rna Virus ◽  
Phytopathogenic Fungus ◽  
First Report ◽  
The Family ◽  
Fusarium Langsethiae

ABSTRACT We describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae. The properties of FlHV1 permit assignment to the genus Alphahypovirus in the family Hypoviridae. This is the first report of a mycovirus identified in F. langsethiae.

scholarly journals Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Guangyuan Zhao ◽  
Pu Yan ◽  
Wentao Shen ◽  
Decai Tuo ◽  
Xiaoying Li ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
Genetically Modified ◽  
Hainan Island ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
Sequence Identity

The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

scholarly journals Coding-Complete Sequence of a SARS-CoV-2 B.1.1.25 Lineage Obtained from an 8-Day-Old Deceased Neonate

2021 ◽  
Vol 10 (35) ◽  
Author(s):  
Muntasir Alam ◽  
M. Ishrat Jahan ◽  
Shafina Jahan ◽  
Dianna M. Blau ◽  
Afruna Rahman ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Complete Sequence ◽  
Nasopharyngeal Swab

We report the complete genome sequence of a SARS-CoV-2 strain, hCoV-19/Bangladesh/icddrb-CHAMPS-BDAA02205/2021, obtained from a nasopharyngeal swab from a deceased neonate from Faridpur, Bangladesh. The strain belongs to lineage B.1.1.25 (a Bangladeshi lineage) but contains some notable mutations similar to those in lineage B.1.1.7.

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