scholarly journals Molecular Characterization of a Novel Endornavirus Conferring Hypovirulence in Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA Strain GD-2

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 178 ◽  
Author(s):  
Li Zheng ◽  
Canwei Shu ◽  
Meiling Zhang ◽  
Mei Yang ◽  
Erxun Zhou
Keyword(s):  
Rhizoctonia Solani ◽  
Rna Virus ◽  
Virulent Strain ◽  
Horizontal Transmission ◽  
Hypothetical Protein ◽  
Complete Sequence ◽  
Sheath Blight ◽  
Rice Sheath Blight ◽  
Transmission Experiment ◽  
Hypovirulent Strain

The complete sequence and genome organization of a novel Endornavirus from the hypovirulent strain GD-2 of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight, were identified using a deep sequencing approach and it was tentatively named as Rhizoctonia solani endornavirus 1 (RsEV1). It was composed of only one segment that was 19,936 bp in length and was found to be the longest endornavirus genome that has been reported so far. The RsEV1 genome contained two open reading frames (ORFs): ORF1 and ORF2. ORF1 contained a glycosyltransferase 1 domain and a conserved RNA-dependent RNA polymerase domain, whereas ORF2 encoded a conserved hypothetical protein. Phylogenetic analysis revealed that RsEV1 was phylogenetically a new endogenous RNA virus. A horizontal transmission experiment indicated that RsEV1 could be transmitted from the host fungal strain GD-2 to a virulent strain GD-118P and resulted in hypovirulence in the derivative isogenic strain GD-118P-V1. Metabolomic analysis showed that 32 metabolites were differentially expressed between GD-118P and its isogenic hypovirulent strain GD-118P-V1. The differential metabolites were mainly classified as organic acids, amino acids, carbohydrates, and the intermediate products of energy metabolism. Pathway annotation revealed that these 32 metabolites were mainly involved in pentose and glucuronate interconversions and glyoxylate, dicarboxylate, starch, and sucrose metabolism, and so on. Taken together, our results showed that RsEV1 is a novel Endornavirus, and the infection of virulent strain GD-118P by RsEV1 caused metabolic disorders and resulted in hypovirulence. The results of this study lay a foundation for the biocontrol of rice sheath blight caused by R. solani AG1-IA.

Effects of straw incorporation on Rhizoctonia solani inoculum in paddy soil and rice sheath blight severity

2013 ◽  
Vol 152 (5) ◽  
pp. 741-748 ◽  
Author(s):  
H. ZHU ◽  
Z. X. WANG ◽  
X. M. LUO ◽  
J. X. SONG ◽  
B. HUANG
Keyword(s):  
Rhizoctonia Solani ◽  
Disease Severity ◽  
Rice Straw ◽  
Paddy Soil ◽  
Sheath Blight ◽  
Soil Productivity ◽  
Rice Sheath Blight ◽  
Important Method ◽  
Pathogen Dynamics ◽  
Straw Incorporation

SUMMARYIncorporation of rice straw into soil has traditionally been an important method of recycling nutrients and improving soil productivity. Currently, although the effects of straw incorporation on disease severity have been documented, the dynamics of the pathogen in soil after straw incorporation are poorly understood. In the present study, rice straw with various proportions of diseased straw was incorporated at three separate locations (SuPu town, SuSong County and FengYang County) in Anhui province, China. The pathogen dynamics in paddy soil and disease severity of sheath blight during two continuous years from April 2010 to April 2012 were investigated. For all three locations, the amount of pathogen inoculum that persisted in the soil increased with increases in the proportion of diseased straw incorporated. Incorporation of 0·3 and 0·5 diseased straw into soil increased the amount of pathogen inoculum in the soil significantly, whereas incorporation of 0·1 diseased straw into soil had no significant effect on the pathogen inoculum compared with the control (no straw incorporated) or disease severity. Incorporation of healthy rice straw (no disease) resulted in a significant decrease in disease severity, whereas proportions of 0·3 and 0·5 diseased straw resulted in a significant increase of disease severity compared with the control. These results suggested that incorporation of diseased straw enhanced pathogen numbers in soil during the whole decomposition period and increased disease severity. To avoid soil-borne disease accumulation, severely diseased straw should be removed from the field or pre-treated before incorporation.

Functional analysis of polygalacturonase gene RsPG2 from Rhizoctonia solani, the pathogen of rice sheath blight

2017 ◽  
Vol 149 (2) ◽  
pp. 491-502 ◽  
Author(s):  
Xijun Chen ◽  
Li Lili ◽  
Yun Zhang ◽  
Jiahao Zhang ◽  
Shouqiang Ouyang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Rice Sheath Blight ◽  
Polygalacturonase Gene

scholarly journals Rhizoctonia solani AG-1 IA infects both rice and signalgrass in the Colombian Llanos

2016 ◽  
Vol 46 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Lina Maria Ramos-Molina ◽  
Edisson Chavarro-Mesa ◽  
Danilo Augusto dos Santos Pereira ◽  
María del Rosario Silva-Herrera ◽  
Paulo Cezar Ceresini
Keyword(s):  
Rhizoctonia Solani ◽  
Species Complex ◽  
Molecular Detection ◽  
Sheath Blight ◽  
Pathogenicity Test ◽  
Rice Sheath Blight ◽  
Specific Primers ◽  
5.8S Rdna ◽  
Foliar Blight ◽  
Rhizoctonia Oryzae

ABSTRACT Foliar blight and death of signalgrass (Urochloa spp.) pastures are caused by the Rhizoctonia solani fungus. This study aimed at determining which pathogens from the Rhizoctonia species complex are associated with leaf and sheath blight in Urochloa and rice, in the Colombian Llanos. Sympatric areas of Urochloa pastures adjacent to rice cropping areas were sampled using a linear transect system. The pathogens were identified using morphological traits, molecular detection based on specific primers and sequencing of the ITS-5.8S rDNA region. R. solani AG-1 IA predominated as the pathogen associated with foliar blight in all samples from U. brizantha cv. 'Toledo' and hybrid Urochloa cv. 'Mulato'. Besides R. solani AG-1 IA (18 % of the samples), Rhizoctonia oryzae-sativae (71 %) and Sclerotium hydrophilum (11 %) were also detected. In the cross-pathogenicity test, the R. solani AG-1 IA fungus was the most aggressive to Urochloa, while R. oryzae-sativae produced very mild infection symptoms. This is the first report of R. oryzae-sativae and S. hydrophilum associated with the complex of rice sheath blight diseases in Colombia.

Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani

2012 ◽  
Vol 34 (12) ◽  
pp. 2291-2298 ◽  
Author(s):  
He Liu ◽  
Wenxiao Tian ◽  
Bin Li ◽  
Guoxing Wu ◽  
Muhammad Ibrahim ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Rice Sheath Blight ◽  
Antifungal Effect

scholarly journals Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India

2005 ◽  
Vol 112 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Celeste C. Linde ◽  
Marcello Zala ◽  
R.S. David Paulraj ◽  
Bruce A. McDonald ◽  
Sam S. Gnanamanickam
Keyword(s):  
Population Structure ◽  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Rice Sheath Blight
Sign in / Sign up

Export Citation Format

Share Document