scholarly journals Impact of Two Reoviruses and Their Coinfection on the Rice RNAi System and vsiRNA Production

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 594 ◽  
Author(s):  
Zhanbiao Li ◽  
Tong Zhang ◽  
Xiuqin Huang ◽  
Guohui Zhou
Keyword(s):  
Rna Interference ◽  
Hot Spots ◽  
Rna Polymerases ◽  
The Other ◽  
Dwarf Virus ◽  
Rnai Pathway ◽  
The Family ◽  
Synergistic Mechanism ◽  
Interfering Rna ◽  
Rice Ragged Stunt Virus

Both Southern rice black-streaked dwarf virus (SRBSDV) and Rice ragged stunt virus (RRSV) belong to the family Reoviridae, and synergistic infection of these two viruses commonly occurs in the field. This study revealed that both SRBSDV and RRSV affect the RNA interference (RNAi) pathway and form different virus-derived interfering RNA (vsiRNA) profiles in rice. Co-infection of rice by SRBSDV and RRSV up-regulated the expression of rice DICER-like (DCL) proteins but down-regulated the expression of rice RNA-dependent RNA polymerases (RDRs), and the accumulation of vsiRNAs of either RBSDV or RRSV was decreased compared with that in singly infected plants. The majority of SRBSDV vsiRNAs were 21 nt or 22 nt in length, whether plants were singly infected with SRBSDV or co-infected with RRSV. On the other hand, the majority of RRSV vsiRNAs were 20 nt, 21 nt, or 22 nt in length, among which those 20 nt in length accounted for the largest proportion; co-infection with SRBSDV further increased the proportion of 20 nt vsiRNAs and decreased the proportion of 21 nt vsiRNAs. Co-infection had no effects on the strand favoritism and hot spots of the vsiRNAs, but changed the bias of the 5′ terminal nucleotide significantly. This study provides a reference for further study on the pathogenesis and synergistic mechanism of SRBSDV and RRSV.

scholarly journals Small Interfering RNA- Modern Approach for Intervening Pathological Conditions

2021 ◽  
Vol 2 (2) ◽  
pp. 16-19
Author(s):  
Shazia Choudhary ◽  
Sheeba Murad ◽  
Sana Gul ◽  
Hayat Khan ◽  
Samra Khalid ◽  
...  
Keyword(s):  
Rna Interference ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Rnai Pathway ◽  
Modern Approach ◽  
Rna Molecules ◽  
Rnai Technology ◽  
Efficient Delivery ◽  
Biological Discovery ◽  
Interfering Rna

RNA interference (RNAi) refers to the inhibition of gene expression by small double-stranded RNA molecules. This technology can prove to be a breakthrough biological discovery of the decade as it has the potential to revolutionize the field of therapeutics. RNA interference (RNAi) through small interfering RNA (siRNA) is currently being evaluated for its efficacy to be used in therapeutics as well as prophylactic strategies. Many studies are being conducted across the globe to optimize the siRNA delivery systems (in terms of safe, stable and efficient delivery) in various disorders. There are a number of diseases such as autoimmune diseases, cancer associated pathological changes, bacterial and viral induced disorders, where RNAi pathway can be explored and RNAi technology can be used as a tool to intervene such abnormalities. This review is an effort to review latest advancements in the field of siRNA based therapy development and the pits and falls generally encountered in the use of this technology.

scholarly journals Synergism Between Southern rice black-streaked dwarf virus and Rice ragged stunt virus Enhances Their Insect Vector Acquisition

2014 ◽  
Vol 104 (7) ◽  
pp. 794-799 ◽  
Author(s):  
Shu Li ◽  
Han Wang ◽  
Guohui Zhou
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Plant Viruses ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Sogatella Furcifera ◽  
Rice Plants ◽  
The Family ◽  
Low Incidence ◽  
Rice Ragged Stunt Virus

Southern rice black-streaked dwarf virus (SRBSDV), a tentative species in the genus Fijivirus, family Reoviridae, is a novel rice virus transmitted by the white-backed planthopper (Sogatella furcifera). Since its discovery in 2001, SRBSDV has spread rapidly throughout eastern and southeastern Asia and caused large rice losses in China and Vietnam. Rice ragged stunt virus (RRSV) (genus Oryzavirus, family Reoviridae) is a common rice virus vectored by the brown planthopper (Nilaparvata lugens). RRSV is also widely distributed in eastern and southeastern Asia but has not previously caused serious problems in China owing to its low incidence. With SRBSDV's spread, however, RRSV has become increasingly common in China, and is frequently found in co-infection with SRBSDV. In this study, we show that SRBSDV and RRSV interact synergistically, the first example of synergism between plant viruses in the family Reoviridae. Rice plants co-infected with both viruses displayed enhanced stunting, earlier symptoms, and higher virus titers compared with singly infected plants. Furthermore, white-backed and brown planthoppers acquired SRBSDV and RRSV, respectively, from co-infected plants at higher rates. We propose that increased RRSV incidence in Chinese fields is partly due to synergism between SRBSDV and RRSV.

The development of RNA interference (RNAi) in gastrointestinal nematodes

Parasitology ◽  
2012 ◽  
Vol 139 (5) ◽  
pp. 605-612 ◽  
Author(s):  
MURRAY E. SELKIRK ◽  
STANLEY C. HUANG ◽  
DAVID P. KNOX ◽  
COLLETTE BRITTON
Keyword(s):  
Rna Interference ◽  
Gene Transcription ◽  
Mode Of Delivery ◽  
Gastrointestinal Nematodes ◽  
Rnai Pathway ◽  
Parasitic Nematodes ◽  
Future Directions ◽  
C Elegans ◽  
Unpublished Work ◽  
Interfering Rna

SUMMARYDespite the utility of RNAi for defining gene function in Caenorhabditis elegans and early successes reported in parasitic nematodes, RNAi has proven to be stubbornly inconsistent or ineffective in the animal parasitic nematodes examined to date. Here, we summarise some of our experiences with RNAi in parasitic nematodes affecting animals and discuss the available data in the context of our own unpublished work, taking account of mode of delivery, larval activation, site of gene transcription and the presence/absence of essential RNAi pathway genes as defined by comparisons to C. elegans. We discuss future directions briefly including the evaluation of nanoparticles as a means to enhance delivery of interfering RNA to the target worm tissue.

scholarly journals Drosophila Dicer-2 has an RNA interference–independent function that modulates Toll immune signaling

2015 ◽  
Vol 1 (9) ◽  
pp. e1500228 ◽  
Author(s):  
Zhaowei Wang ◽  
Di Wu ◽  
Yongxiang Liu ◽  
Xiaoling Xia ◽  
Wanyun Gong ◽  
...  
Keyword(s):  
Rna Interference ◽  
Posttranscriptional Regulation ◽  
Protein Translation ◽  
Binding Activity ◽  
Rnai Pathway ◽  
Independent Function ◽  
Immune Signaling ◽  
Toll Signaling ◽  
Rna Biogenesis ◽  
Interfering Rna

Dicer-2 is the central player for small interfering RNA biogenesis in the Drosophila RNA interference (RNAi) pathway. Intriguingly, we found that Dicer-2 has an unconventional RNAi-independent function that positively modulates Toll immune signaling, which defends against Gram-positive bacteria, fungi, and some viruses, in both cells and adult flies. The loss of Dicer-2 expression makes fruit flies more susceptible to fungal infection. We further revealed that Dicer-2 posttranscriptionally modulates Toll signaling because Dicer-2 is required for the proper expression of Toll protein but not for Toll protein stability or Toll mRNA transcription. Moreover, Dicer-2 directly binds to the 3′ untranslated region (3′UTR) of Toll mRNA via its PAZ (Piwi/Argonaute/Zwille) domain and is required for protein translation mediated by Toll 3′UTR. The loss of Toll 3′UTR binding activity makes Dicer-2 incapable of promoting Toll signaling. These data indicate that the interaction between Dicer-2 and Toll mRNA plays a pivotal role in Toll immune signaling. In addition, we found that Dicer-2 is also required for the Toll signaling induced by two different RNA viruses in Drosophila cells. Consequently, our findings uncover a novel RNAi-independent function of Dicer-2 in the posttranscriptional regulation of Toll protein expression and signaling, indicate an unexpected intersection of the RNAi pathway and the Toll pathway, and provide new insights into Toll immune signaling, Drosophila Dicer-2, and probably Dicer and Dicer-related proteins in other organisms.

Problems of Marriage and Self Surrender in The Novels of Shashi Deshpande

2019 ◽  
Vol 7 (11) ◽  
pp. 18
Author(s):  
Dr. Indu Goyal
Keyword(s):  
Middle Class ◽  
The Other ◽  
Important Thing ◽  
Female Protagonists ◽  
Central Concern ◽  
Modern Age ◽  
The Family ◽  
Traditional Marriage ◽  
Shashi Deshpande ◽  
Middle Class People

Marriage is an important thing in the life of a woman. The importance that our society attaches to marriage is reflected in our literature and it is the central concern of Shashi Deshpade’s novels. In our society where girl learns early that she is ‘Paraya Dhan’, and she is her parents’ responsibility till the day she is handed over to her rightful owners. What a girl makes of her life, how she shapes herself as an individual, what profession she takes up is not as important as whom she marries. Marriage is the ultimate goal of a woman’s life. This paper attempts to probe into the problems of marriage through the protagonists of her novels where one enjoys the freedom of marriage and the other accepts the traditional marriage. Shashi Deshpade highlights the problems of marriage faced by middle-class people in finding suitable grooms for their daughters. This problem is well-illustrated through the characters of her novels. Since the girl’s mind over her childhood is tuned that she is another’s property, she tries to attach a lot of importance to it. it is indeed a tragedy that even in the modern age, Indian females echo the same sentiment where it was marriage which mattered most of them but not to the men. It is a beginning of females sacrifices in life that marriage brings to her. Shashi Deshpande encourages her female protagonists to rise in rebellion against the males in the family matters, instead she wants to build a harmonious relationship between man and woman in a mood of compromise and reconciliation.  

On Commutation Properties of the Composition Relation of Convergent and Divergent Permutations (Part I)

2014 ◽  
Vol 58 (1) ◽  
pp. 13-22
Author(s):  
Roman Wituła ◽  
Edyta Hetmaniok ◽  
Damian Słota
Keyword(s):  
Finite Order ◽  
Convergent Series ◽  
The Other ◽  
Other Hand ◽  
The Family ◽  
Composition Relation ◽  
The Continuum

Abstract In the paper we present the selected properties of composition relation of the convergent and divergent permutations connected with commutation. We note that a permutation on ℕ is called the convergent permutation if for each convergent series ∑an of real terms, the p-rearranged series ∑ap(n) is also convergent. All the other permutations on ℕ are called the divergent permutations. We have proven, among others, that, for many permutations p on ℕ, the family of divergent permutations q on ℕ commuting with p possesses cardinality of the continuum. For example, the permutations p on ℕ having finite order possess this property. On the other hand, an example of a convergent permutation which commutes only with some convergent permutations is also presented.

The family Orthotrichaceae (Bryophyta) in flora of Russia: results of revision and biogeographical review

2018 ◽  
Vol 52 (2) ◽  
pp. 519-534 ◽  
Author(s):  
V. E. Fedosov
Keyword(s):  
Hot Spots ◽  
Latitudinal Gradient ◽  
Hot Spot ◽  
Spatial Differentiation ◽  
Taxonomic Composition ◽  
Undescribed Species ◽  
Longitudinal Gradient ◽  
Biogeographic Pattern ◽  
The Family ◽  
North Pacific Region

Recent studies on Orthotrichoid mosses in Russia are summarized genus by genus. Orthotrichum furcatum Otnyukova is synonymized with Nyholmiella obtusifolia. Orthotrichum vittii is excluded from the Russian moss flora. Description of O. dagestanicum is amended. Fifty four currently recognized species from 9 genera of the Orthotrichaceae are presently known to occur in Russia; list of species with common synonyms and brief review of distribution in Russia is presented. Numerous problematic specimens with unresolved taxonomy were omitted for future. Revealed taxonomical inconsistencies in the genera Zygodon, Ulota, Lewinskya, Nyholmiella, Orthotrichum are briefly discussed. Main regularities of spatial differentiation of the family Orthotrichaceae in Russia are considered. Recently presented novelties contribute to the certain biogeographic pattern, indicating three different centers of diversity of the family, changing along longitudinal gradient. Unlike European one, continental Asian diversity of Orthotrichaceae is still poorly known, the Siberian specimens which were previously referred to European species in most cases were found to represent other, poorly known or undescribed species. North Pacific Region houses peculiar and poorly understood hot spot of diversity of Orthotrichoid mosses. Thus, these hot spots are obligatory to be sampled in course of revisions of particular groups, since they likely comprise under-recorded cryptic- or semi-cryptic species. Latitudinal gradient also contributes to the spatial differentiation of the revealed taxonomic composition of Orthotrichaceae.

Status of Family Fissidentaceae in Gujarat

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
D.G. Shah ◽  
D.N. Mehta ◽  
R.V. Gujar
Keyword(s):  
Distinct Species ◽  
The State ◽  
The Other ◽  
Land Plants ◽  
Reproductive Structures ◽  
The Family ◽  
The Status ◽  
Vegetative Characters ◽  
First Time ◽  
Different Parts

Bryophytes are the second largest group of land plants and are also known as the amphibians of the plant kingdom. 67 species of bryophytes have been reported from select locations across the state of Gujrat. The status of family fissidentaceae which is a large moss family is being presented in this paper. Globally the family consists of 10 genera but only one genus, Fissidens Hedw. has been collected from Gujarat. Fissidens is characterized by a unique leaf structure and shows the presence of three distinct lamina, the dorsal, the ventral and the vaginant lamina. A total of 8 species of Fissidens have been reported from the state based on vegetative characters as no sporophyte stages were collected earlier. Species reported from the neighboring states also showed the absence of sporophytes. The identification of different species was difficult due to substantial overlap in vegetative characters. Hence a detailed study on the diversity of members of Fissidentaceae in Gujarat was carried out between November 2013 and February 2015. In present study 8 distinct species of Fissidens have been collected from different parts of the state. Three species Fissidens splachnobryoides Broth., Fissidens zollingerii Mont. and Fissidens curvato-involutus Dixon. have been identified while the other five are still to be identified. Fissidens zollingerii Mont. and Fissidens xiphoides M. Fleisch., which have been reported as distinct species are actually synonyms according to TROPICOS database. The presence of sexual reproductive structures and sporophytes for several Fissidens species are also being reported for the first time from the state.

Women-Friendly Policies being adopted by Organizations: An Innovative aspect

2016 ◽  
Vol 3 (01) ◽  
Author(s):  
Shlesha Singh ◽  
Mrinalini Pandey
Keyword(s):  
Sexual Harassment ◽  
Glass Ceiling ◽  
Gender Stereotype ◽  
The Other ◽  
Family Responsibilities ◽  
Other Hand ◽  
The Family ◽  
Workplace Barriers ◽  
Women In The Workforce ◽  
And Gender

Organizations are these days realizing the importance of women in the workforce and to tap that talent, organizations are now-a-days putting extra efforts. Workplaces were designed keeping men in mind and which has been intercepting women from continuing the competitive jobs and career along with the family responsibilities. On the other hand, there are various workplace barriers which are adding to the other problems. Women face several barriers at the workplace like sexual harassment, glass ceiling and gender stereotype.

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