scholarly journals Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses

Viruses ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 564 ◽  
Author(s):  
Iglė Vepštaitė-Monstavičė ◽  
Juliana Lukša ◽  
Aleksandras Konovalovas ◽  
Dovilė Ežerskytė ◽  
Ramunė Stanevičienė ◽  
...  
Keyword(s):  
Gene Products ◽  
Wild Type ◽  
Spontaneous Fermentation ◽  
Saccharomyces Paradoxus ◽  
The Family ◽  
Different Types ◽  
Killer System ◽  
Virus Sequence ◽  
First Time ◽  
Satellite Viruses

The Saccharomycetaceae yeast family recently became recognized for expanding of the repertoire of different dsRNA-based viruses, highlighting the need for understanding of their cross-dependence. We isolated the Saccharomyces paradoxus AML-15-66 killer strain from spontaneous fermentation of serviceberries and identified helper and satellite viruses of the family Totiviridae, which are responsible for the killing phenotype. The corresponding full dsRNA genomes of viruses have been cloned and sequenced. Sequence analysis of SpV-LA-66 identified it to be most similar to S. paradoxus LA-28 type viruses, while SpV-M66 was mostly similar to the SpV-M21 virus. Sequence and functional analysis revealed significant differences between the K66 and the K28 toxins. The structural organization of the K66 protein resembled those of the K1/K2 type toxins. The AML-15-66 strain possesses the most expressed killing property towards the K28 toxin-producing strain. A genetic screen performed on S. cerevisiae YKO library strains revealed 125 gene products important for the functioning of the S. paradoxus K66 toxin, with 85% of the discovered modulators shared with S. cerevisiae K2 or K1 toxins. Investigation of the K66 protein binding to cells and different polysaccharides implies the β-1,6 glucans to be the primary receptors of S. paradoxus K66 toxin. For the first time, we demonstrated the coherent habitation of different types of helper and satellite viruses in a wild-type S. paradoxus strain.

Branch Reconfiguration of Bricard Linkages Based on Toroids Intersections: Line-Symmetric Case

2018 ◽  
Vol 10 (3) ◽  
Author(s):  
P. C. López-Custodio ◽  
J. S. Dai ◽  
J. M. Rico
Keyword(s):  
Symmetric Case ◽  
Plane Symmetric ◽  
Bifurcation Points ◽  
The Family ◽  
Different Types ◽  
The Common ◽  
First Time ◽  
Made In

This paper for the first time investigates a family of line-symmetric Bricard linkages by means of two generated toroids and reveals their intersection that leads to a set of special Bricard linkages with various branches of reconfiguration. The discovery is made in the concentric toroid–toroid intersection. By manipulating the construction parameters of the toroids, all possible bifurcation points are explored. This leads to the common bi-tangent planes that present singularities in the intersection set. The study reveals the presence of Villarceau and secondary circles in the toroid–toroid intersection. Therefore, a way to reconfigure the Bricard linkage to a pair of different types of Bennett linkage is uncovered. Further, a linkage with two Bricard and two Bennett motion branches is explored. In addition, the paper reveals the Altmann linkage as a member of the family of special line-symmetric Bricard linkage studied in this paper. The method is applied to the plane-symmetric case in the following paper published together with this paper.

scholarly journals Isolation of flavonoids from Anemopaegma arvense (Vell) Stellf. ex de Souza and their antifungal activity against Trichophyton rubrum

2013 ◽  
Vol 49 (3) ◽  
pp. 559-565 ◽  
Author(s):  
Camila Di Giovane Costanzo ◽  
Vanessa Colnaghi Fernandes ◽  
Sônia Zingaretti ◽  
Rene Oliveira Beleboni ◽  
Ana Maria Soares Pereira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Cytotoxic Activity ◽  
Tumor Cells ◽  
Trichophyton Rubrum ◽  
Wild Type ◽  
Minimum Inhibitory Concentrations ◽  
The Family ◽  
First Time

Anemopaegma arvense (Vell) Stellf. ex de Souza belongs to the family Bignoniaceae, and is popularly known as catuaba. To evaluate the cytotoxic and antimicrobial activity of A. arvense, fraction F3 and flavonoids 1 (quercetin 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside) (rutin) and flavonoid 2 (quercetin 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside) were isolated from the leaves of this plant. Fraction F3 and flavonoids 1 and 2 exhibited no antibacterial activity. Furthermore, no cytotoxic activity of fraction 3 or flavonoids 1 and 2 was observed against the tumor cells tested. However, analysis of the antifungal activity of flavonoids 1 and 2 revealed minimum inhibitory concentrations of 0.5 and 0.25 mg/mL, respectively, against the Trichophyton rubrum strains tested (wild type and mutant). This study demonstrates for the first time the antifungal activity of isolated flavonoids, validating the same activity for A. arvense.

scholarly journals The development of familys structure in Finland in 1960-1987

1992 ◽  
pp. 5-17
Author(s):  
Matti Saari
Keyword(s):  
Young Children ◽  
Family Structure ◽  
Age Groups ◽  
Children And Families ◽  
The Family ◽  
Family Types ◽  
Sample Data ◽  
Different Types ◽  
First Time ◽  
Total Coverage

The purpose of the article is to study the development of family structure in Finland in 1960—1987. For the first time, the data provide total coverage of all family types. Cohabitation became increasingly common in the 1970s but has so far not been covered by the data of official family statistics. Further, sample data have only included family data on selected age groups. Statistics Finland has now developed a method by which family and cohabitation data may automatically be derived from information about people’s place of residence, demographic characteristics, and relatives. The family statistics data for 1981 and 1987 have been reproduced. The article describes changes in the numbers of families of different types, families with young children and families of different sizes, as well as developments in the regional family structure during the 1960—1987 period. In addition, developments in the prevalence of living in a family and living in a family with young children are studied with reference to the 1981—1987 period.

To the species structure of predatory phytoseiid mites (Parasitiformes, Phytoseiidae) of state reserve “Elanetskyi Step”

2018 ◽  
Vol 14 (1) ◽  
pp. 39-43
Author(s):  
V. Yu. Bondarev
Keyword(s):  
Common Species ◽  
Species Structure ◽  
Phytoseiid Mites ◽  
First Order ◽  
Plant Feeding ◽  
Plant Associations ◽  
The Family ◽  
Different Types ◽  
First Time ◽  
Predatory Phytoseiid Mites

As material for this work the gatherings of the author executed in 2016 year. On 43 species of plants 85 samples in which 249 copies of phytoseiid mites are revealed are taken. For the statistical analysis used an occurrence index (P1, %) and an index of domination of Paliya-Kovnatski (Di). Species structure of predatory phytoseiid mites (Parasitiformes: Phytoseiidae) in plant associations of state reserve "Elanetskyi step" (Mykolaiv region, Ukraine) were studied at the first time. 21 species of 7 genera of the family are detected (A. andersoni, A. herbarius, A. maior, N. marginatus, N. reductus, N. tauricus, E. finlandicus, D. echinus, T. tiliarum, P. intermixtus, P. soleiger, A. caudiglans, A. rapida, A. pirianykae, A. recki, A. verrucosa, A. spectata, T. cotoneastri, T. laurae, T. tiliae). Frequency of occurrence and fidelity to different types of plant communities for each species of mites were determined. Amlydromella (s. str.) recki, the most common species, was with occurrence index 35.3%. The species Amblydromella (s. str.) rapida, Amblydromella (Litoseia) spectata, and Paraseiulus intermixtus were the rare species (one specimen of each species was presented at collections). Amblydromella (s. str.) recki was the dominant in the complex of plant-feeding predatory mites. Three species, A. pirianykae, A. andersoni and E. finlandicus, were the subdominants. Typhlodromus cotoneastri was the subdominant of the first order. The remaining 16 species were the secondary members of the complex.

scholarly journals A Novel Biallelic STING1 Gene Variant Causing SAVI in Two Siblings

2021 ◽  
Vol 11 ◽  
Author(s):  
Malak Ali Alghamdi ◽  
Jaazeel Mulla ◽  
Narjes Saheb Sharif-Askari ◽  
Francisco J. Guzmán-Vega ◽  
Stefan T. Arold ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Elevated Serum ◽  
Janus Kinase ◽  
Wild Type ◽  
Janus Kinase Inhibitor ◽  
Constitutive Activation ◽  
Beta Interferon ◽  
The Family ◽  
First Time ◽  
Control Family

STING-associated vasculopathy of infantile-onset (SAVI) is one of the newly identified types of interferonopathies. SAVI is caused by heterozygous gain-of-function mutations in the STING1. We herein report for the first time a homozygous variant in the STING1 gene in two siblings that resulted in constitutive activation of STING gene and the SAVI phenotype. Exome sequencing revealed a novel homozygous NM_198282.3: c.841C>T; p.(Arg281Trp) variant in exon 7 of the STING1 gene. The variant segregated in the family to be homozygous in all affected and either heterozygous or wild type in all healthy. Computational structural analysis of the mutants revealed changes in the STING protein structure/function. Elevated serum beta-interferon levels were observed in the patients compared to the control family members. Treatment with Janus kinase inhibitor (JAK-I) Ruxolitinib suppressed the inflammatory process, decreased beta-interferon levels, and stopped the progression of the disease.

Identification of a baculovirus polyhedron formation mutant with a novel phenotype

1996 ◽  
Vol 54 ◽  
pp. 796-797
Author(s):  
James M. Slavicek ◽  
Melissa J. Mercer ◽  
Mary Ellen Kelly
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
Type Number ◽  
Infection Cycle ◽  
Wild Type ◽  
Protein Matrix ◽  
Insect Midgut ◽  
Viral Particles ◽  
Budded Virus ◽  
Viral Form

Nucleopolyhedroviruses (NPV, family Baculoviridae) produce two morphological forms, a budded virus form and a viral form that is occluded into a paracrystalline protein matrix. This structure is termed a polyhedron and is composed primarily of the protein polyhedrin. Insects are infected by NPVs after ingestion of the polyhedron and release of the occluded virions through dissolution of the polyhedron in the alkaline environment of the insect midgut. Early after infection the budded virus form is produced. It buds through the plasma membrane and then infects other cells. Later in the infection cycle the occluded form of the virus is generated (reviewed by Blissard and Rohrmann, 1990).The processes of polyhedron formation and virion occlusion are likely to involve a number of viral gene products. However, only two genes, the polyhedrin gene and 25K FP gene, have been identified to date that are necessary for the wild type number of polyhedra to be formed and viral particles occluded.

Buried Narratives: Exhuming the Mother's Story in Oliver Twist

2018 ◽  
Vol 8 (3) ◽  
pp. 247-266
Author(s):  
Michelle L. Wilson
Keyword(s):  
Social Change ◽  
Maternal Inheritance ◽  
The Novel ◽  
Poor Law ◽  
The Family ◽  
The Law ◽  
First Time ◽  
Critical Consideration

Initially, Oliver Twist (1839) might seem representative of the archetypal male social plot, following an orphan and finding him a place by discovering the father and settling the boy within his inheritance. But Agnes Fleming haunts this narrative, undoing its neat, linear transmission. This reconsideration of maternal inheritance and plot in the novel occurs against the backdrop of legal and social change. I extend the critical consideration of the novel's relationship to the New Poor Law by thinking about its reflection on the bastardy clauses. And here, of course, is where the mother enters. Under the bastardy clauses, the responsibility for economic maintenance of bastard children was, for the first time, legally assigned to the mother, relieving the father of any and all obligation. Oliver Twist manages to critique the bastardy clauses for their release of the father, while simultaneously embracing the placement of the mother at the head of the family line. Both Oliver and the novel thus suggest that it is the mother's story that matters, her name through which we find our own. And by containing both plots – that of the father and the mother – Oliver Twist reveals the violence implicit in traditional modes of inheritance in the novel and under the law.

The Arabic Origins of English and Indo-European “Definite Articles”: A Radical Linguistic Theory Approach

2016 ◽  
Vol 4 (6) ◽  
pp. 26
Author(s):  
Zaidan Ali Jassem
Keyword(s):  
Comparative Method ◽  
Linguistic Theory ◽  
General Level ◽  
Theory Approach ◽  
Tree Model ◽  
Linguistic Change ◽  
European Languages ◽  
The Family ◽  
Different Types ◽  
Definite Articles

This paper traces the Arabic origins or cognates of the “definite articles” in English and Indo-European languages from a radical linguistic (or lexical root) theory perspective. The data comprises the definite articles in English, German, French, Spanish, Portuguese, Italian, Romanian, Latin, Greek, Macedonian, Russian, Polish, Sanskrit, Hindi, Bengali, Persian, and Arabic. The results clearly indicate that five different types of such articles emerged in the data, all of which have true Arabic cognates with the same or similar forms and meanings, whose differences are due to natural and plausible causes and different routes of linguistic change, especially lexical, semantic, or morphological shift. Therefore, the results support the adequacy of the radical linguistic theory according to which, unlike the Family Tree Model or Comparative Method, Arabic, English, German, French, Latin, Greek, and Sanskrit not only belong to the same language family, renamed Eurabian or Urban family, but also are dialects of the same language, with Arabic being their origin all because only it shares the whole cognates with them all and because it has a huge phonetic, morphological, grammatical, and lexical variety. They also manifest fundamental flaws and grave drawbacks which plague English and Indo-European lexicography for ignoring Arabic as an ultimate ancestor and progenitor not only in the treatment of the topic at hand but in all others in general. On a more general level, they also show that there is a radical language from which all human languages stemmed and which has been preserved almost intact in Arabic, thus being the most conservative and productive language

Faktor-Faktor Determinan yang melatar belakangi Kehamilan Remaja di Desa Pandansari Kecamatan Poncokusumo Kabupaten Malang

2020 ◽  
Vol 7 (2) ◽  
pp. 247-255
Author(s):  
Maria Magdalena Setyaningsih ◽  
Emy Sutiyarsih
Keyword(s):  
Sex Education ◽  
Teen Pregnancy ◽  
Teenage Pregnancy ◽  
Well Being ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
The Family ◽  
History Of ◽  
First Time

Kehamilan remaja adalah kehamilan yang terjadi pada remaja berusia kurang dari 20 tahunan. Kehamilan remaja memberikan banyak kerugian bagi kesehatan, mental dan psikologis, kesejahteraan ekonomi dan peluang karier, kemiskinan dan prospek kehidupan masa depan remaja. Tujuan penelitian mengidentifikasi faktor determinan yang melatarbelakangi terjadinya kehamilan remaja. Jenis penelitian adalah penelitian analitik kategorik jenis survei kuantitatif dengan desain case control. Populasi semua perempuan yang bertempat tinggal di wilayah dusun Wonosari, Sukosari, dan Krajan Pandansari dan pernah/sedang hamil pertama kali pada usia kurang dari 20 tahun. Teknik pengambilan sampel cluster random sampling besar sampel 73. Berdasarkan hasil model akhir analisis multivariat, diketahui bahwa variabel pendidikan, riwayat kehamilan remaja pada keluarga dan usia menikah merupakan variabel yang berhubungan dengan kejadian kehamilan remaja setelah dikontrol oleh variabel akses informasi, responden berpendidikan rendah memiliki peluang 20,8 kali lebih tinggi, responden yang memiliki riwayat kehamilan remaja pada keluarga memiliki peluang 14,9 kali lebih tinggi, responden yang menikah pada usia <20 tahun memiliki peluang 12,1 kali lebih tinggi, responden dengan pemahaman yang kurang baik terkait penggunaan kondom memiliki peluang 5,9 kali lebih tinggi untuk terjadi kehamilan remaja. Oleh karena itu perlu dibangun karakter buiding, sosial karakter suport untuk para ibu remaja dan keluarga sehingga terbangun interaksi yang baik dalam keluarga yang dilandasi dengan pendidikan dan pemahaman yang baik tentang Pendidikan seksualitas. Teen pregnancy is a pregnancy that occurs in adolescents aged less than 20 years old. Teen pregnancy provides many disadvantages for health, mental, psychological, economic well-being, career opportunities, poverty, and the future life. The aim of the study was to identify the determinants underlying teen pregnancy incidence. This study was a quantitative study with categorical analytic method. The study design used a case control with two comparison groups. The groups were control group and case group. The population in this study was all women who lived in the Wonosari, Sukosari, and Krajan Pandansari district and had or were pregnant for the first time at the age of less than 20 years old. Seventy three respondents were recruited using cluster sampling technique. The case group consists of women who were or had pregnant for the first time at the age of less than 20 years old and their children are currently aged ≤ 1 years old. The control group consists of women who were pregnant for the first time at the age of > 20 years old. The data was collected using a questionnaire. The findings showed that education, history of teen pregnancy in family and the age of marriage were related to the incidence of teen pregnancy after being controlled by information access. Low-educated respondents had 20.8 times higher chance of experiencing teen pregnancy; respondents with a history of teen pregnancy in the family had 4.9 times higher chance of experiencing teen pregnancy; respondents who were married at the age of < 20 years old had 12.1 times higher chance of experiencing teen pregnancy; respondents with poor understanding of condom use had 5.9 times higher chance of teenage pregnancy. In conclusion, the findings suggest to build good interactions in the family based on education and a good understanding of sex education.

New records of some rare species in the family Hyaloscyphaceae (Ascomycota, Helotiales) from Central Russia

2013 ◽  
Vol 47 ◽  
pp. 135-142
Author(s):  
E. S. Popov
Keyword(s):  
Rare Species ◽  
New Records ◽  
Moscow Region ◽  
North Caucasus ◽  
Central Russia ◽  
The Family ◽  
First Time

Three rare species of discomycetes in the family Hyaloscyphaceae are reported from Central Russia (Oryol and Bryansk Regions). Proliferodiscus tricolor is recorded for the first time in Russia. Comments are made on Aeruginoscyphus sericeus and Eriopezia caesia previously reported only from Moscow Region and North Caucasus respectively.

Sign in / Sign up

Export Citation Format

Share Document