scholarly journals Detection of Usutu, Sindbis, and Batai Viruses in Mosquitoes (Diptera: Culicidae) Collected in Germany, 2011–2016

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 389 ◽  
Author(s):  
Dorothee Scheuch ◽  
Mandy Schäfer ◽  
Martin Eiden ◽  
Eva Heym ◽  
Ute Ziegler ◽  
...  
Keyword(s):  
Sindbis Virus ◽  
Phylogenetic Analyses ◽  
Animal Health ◽  
Sampling Location ◽  
Rna Sequences ◽  
Virus Rna ◽  
Increasing Risk ◽  
Northeastern Germany ◽  
Flooding Events ◽  
Culex Pipiens S.L

Due to the emergence of non-endemic mosquito vectors and the recent outbreaks of mosquito-borne diseases, mosquito-borne pathogens are considered an increasing risk to public and animal health in Europe. To obtain a status quo regarding mosquito-borne viruses and their vectors in Germany, 97,648 mosquitoes collected from 2011 to 2016 throughout the country were screened for arboviruses. Mosquitoes were identified to species, pooled in groups of up to 50 individuals according to sampling location and date, and screened with different PCR assays for Flavi-, Alpha- and Orthobunyavirus RNA. Two pools tested positive for Usutu virus-RNA, two for Sindbis virus-RNA, and 24 for Batai virus-RNA. The pools consisted of Culex pipiens s.l., Culex modestus, Culex torrentium, Culiseta sp., Aedes vexans, Anopheles daciae, and Anopheles messeae mosquitoes and could be assigned to nine different collection sites, with seven of them located in northeastern Germany. Phylogenetic analyses of the viral RNA sequences showed relationships with strains of the viruses previously demonstrated in Germany. These findings confirm continuing mosquito-borne zoonotic arbovirus circulation even though only a rather small percentage of the screened samples tested positive. With respect to sampling sites and periods, virus circulation seems to be particularly intense in floodplains and after flooding events when mosquitoes develop in excessive numbers and where they have numerous avian hosts available to feed on.

Synchronous sensing of three conserved sequences of Zika virus using a DNAs@MOF hybrid: experimental and molecular simulation studies

2019 ◽  
Vol 6 (1) ◽  
pp. 148-152 ◽  
Author(s):  
Bao-Ping Xie ◽  
Gui-Hua Qiu ◽  
Bin Sun ◽  
Zi-Feng Yang ◽  
Wen-Hua Zhang ◽  
...  
Keyword(s):  
Molecular Simulation ◽  
Dna Sequences ◽  
Hybrid Material ◽  
Zika Virus ◽  
Metal Organic Framework ◽  
Simulation Studies ◽  
Synchronous Detection ◽  
Rna Sequences ◽  
Virus Rna ◽  
Metal Organic

A metal–organic framework of Cu(ii) has been prepared and impregnated with three dye-labeled DNA sequences. The hybrid material formed is capable of synchronous detection of three conserved Zika virus RNA sequences.

scholarly journals Efficient Interaction between Arenavirus Nucleoprotein (NP) and RNA-Dependent RNA Polymerase (L) Is Mediated by the Virus Nucleocapsid (NP-RNA) Template

2015 ◽  
Vol 89 (10) ◽  
pp. 5734-5738 ◽  
Author(s):  
Masaharu Iwasaki ◽  
Nhi Ngo ◽  
Beatrice Cubitt ◽  
Juan C. de la Torre
Keyword(s):  
Rna Polymerase ◽  
Gene Transcription ◽  
Viral Genome ◽  
Rna Replication ◽  
Rna Dependent Rna Polymerase ◽  
Rna Sequences ◽  
L Protein ◽  
Virus Rna

In this study, we document that efficient interaction between arenavirus nucleoprotein (NP) and RNA-dependent RNA polymerase (L protein), the twotrans-acting viral factors required for both virus RNA replication and gene transcription, requires the presence of virus-specific RNA sequences located within the untranslated 5′ and 3′ termini of the viral genome.

scholarly journals Viruses Associated with Rusty Mottle and Twisted Leaf Diseases of Sweet Cherry Are Distinct Species

2013 ◽  
Vol 103 (12) ◽  
pp. 1287-1295 ◽  
Author(s):  
D. E. V. Villamor ◽  
K. C. Eastwell
Keyword(s):  
Distinct Species ◽  
Mottle Virus ◽  
Distribution Data ◽  
Virus Species ◽  
Rna Sequences ◽  
Sequence Comparisons ◽  
Protein Coding ◽  
Green Ring ◽  
Virus Rna ◽  
The Family

Virus RNA sequences related to those of the family Betaflexiviridae were amplified from trees affected with the following diseases: cherry twisted leaf, apricot ring pox, cherry necrotic rusty mottle, cherry rusty mottle, and cherry green ring mottle. Phylogenetic analysis of virus sequences obtained from these diseased trees from western North America, along with published sequences of Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV), revealed four major clades. Segregation into these four populations correlated with distinct symptom expression on woody indicators, suggesting that each clade represents a distinct virus species within the family Betaflexiviridae. The viruses occupying each clade were designated clade I: Cherry twisted leaf associated virus, clade II: CNRMV, clade III: Cherry rusty mottle associated virus, and clade IV: CGRMV. Potential recombination events were predicted to occur within and between these viruses, the latter being strongly supported by incongruent phylogenies. Examination of frequency distribution data derived from pairwise sequence comparisons of coat protein coding sequences resulted in a proposal for alternative guidelines for species demarcation for this family of viruses.

scholarly journals Strand-Specific Detection of Hantaan Virus RNA Sequences byIn VitroDNA Amplification

1994 ◽  
Vol 38 (11) ◽  
pp. 905-908
Author(s):  
Yuji Isegawa ◽  
Atsushi Ohshima ◽  
Yoshihiro Sokawa ◽  
Koichi Yamanishi
Keyword(s):  
Hantaan Virus ◽  
Specific Detection ◽  
Rna Sequences ◽  
Virus Rna
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