scholarly journals Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 355 ◽  
Author(s):  
Gentil Bentes ◽  
Juliana Guimarães ◽  
Eduardo Volotão ◽  
Alexandre Fialho ◽  
Cleber Hooper ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Macaca Fascicularis ◽  
Infection Model ◽  
Human Group ◽  
Cynomolgus Monkeys ◽  
Group A Rotavirus ◽  
Group A ◽  
Experimental Infection Model

A pasture-based experimental infection model for footrot in sheep

2020 ◽  
pp. 106305
Author(s):  
Andrew S. McPherson ◽  
Richard J. Whittington ◽  
Ruth M. Kennan ◽  
Julian I. Rood ◽  
Om P. Dhungyel
Keyword(s):  
Experimental Infection ◽  
Infection Model ◽  
Experimental Infection Model

The first identification of rare human group A rotavirus strain G3P[10] with severe infantile diarrhea in eastern India

2012 ◽  
Vol 12 (8) ◽  
pp. 1933-1937 ◽  
Author(s):  
Anupam Mukherjee ◽  
Satarupa Mullick ◽  
Nobumichi Kobayashi ◽  
Mamta Chawla-Sarkar
Keyword(s):  
Eastern India ◽  
Human Group ◽  
Infantile Diarrhea ◽  
Group A Rotavirus ◽  
Group A

scholarly journals Microarrays for Genotyping Human Group A Rotavirus by Multiplex Capture and Type-Specific Primer Extension

2003 ◽  
Vol 41 (11) ◽  
pp. 5153-5158 ◽  
Author(s):  
L. Lovmar ◽  
C. Fock ◽  
F. Espinoza ◽  
F. Bucardo ◽  
A.-C. Syvanen ◽  
...  
Keyword(s):  
Primer Extension ◽  
Human Group ◽  
Specific Primer ◽  
Group A Rotavirus ◽  
Group A

Typing of human group A rotavirus with alkaline phosphatase-labeled oligonucleotide probes

1992 ◽  
Vol 37 (3) ◽  
pp. 192-196 ◽  
Author(s):  
Orntipa Sethabutr ◽  
Suvath Hanchalay ◽  
Udom Lexomboon ◽  
Ruth F. Bishop ◽  
Ian H. Holmes ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Oligonucleotide Probes ◽  
Human Group ◽  
Group A Rotavirus ◽  
Group A

scholarly journals Whip-LAMP: a novel LAMP assay for the detection of Trichuris muris-derived DNA in stool and urine samples in a murine experimental infection model

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Pedro Fernández-Soto ◽  
Carlos Fernández-Medina ◽  
Susana Cruz-Fernández ◽  
Beatriz Crego-Vicente ◽  
Begoña Febrer-Sendra ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Experimental Infection ◽  
Lamp Assay ◽  
Diagnostic Methods ◽  
Urine Samples ◽  
Infection Model ◽  
Trichuris Muris ◽  
Stool Samples ◽  
First Time ◽  
Experimental Infection Model

Abstract Background Trichuris trichiura (human whipworm) infects an estimated 477 million individuals worldwide. In addition to T. trichiura, other Trichuris species can cause an uncommon zoonosis and a number of human cases have been reported. The diagnosis of trichuriasis has relied traditionally on microscopy. Recently, there is an effort to use molecular diagnostic methods, mainly qPCR. LAMP technology could be an alternative for qPCR especially in low-income endemic areas. Trichuris muris, the causative agent of trichuriasis in mice, is of great importance as a model for human trichuriasis. Here, we evaluate the diagnostic utility of a new LAMP assay in an active experimental mouse trichuriasis in parallel with parasitological method by using stool and, for the first time, urine samples. Methods Stool and urine samples were collected from mice infected with eggs of T. muris. The dynamics of infection was determined by counting the number of eggs per gram of faeces. A LAMP based on the 18S rRNA gene from T. muris was designed. Sensitivity and specificity of LAMP was tested and compared with PCR. Stool and urine samples were analysed by both LAMP and PCR techniques. Results Trichuris muris eggs were detected for the first time in faeces 35 days post-infection. LAMP resulted specific and no cross-reactions were found when using 18 DNA samples from different parasites. The detection limit of the LAMP assay was 2 pg of T. muris DNA. When testing stool samples by LAMP we obtained positive results on day 35 p.i. and urine samples showed amplification results on day 20 p.i., i.e. 15 days before the onset of T. muris eggs in faeces. Conclusions To the best of our knowledge, we report, for the first time, a novel LAMP assay (Whip-LAMP) for sensitive detection of T. muris DNA in both stool and urine samples in a well-established mice experimental infection model. Considering the advantages of urine in molecular diagnosis in comparison to stool samples, should make us consider the possibility of starting the use urine specimens in molecular diagnosis and for field-based studies of human trichuriasis where possible. Further studies with clinical samples are still needed.

A decade‐long temporal analyses of human group‐A rotavirus among children with gastroenteritis: Prevaccination scenario in West Bengal, eastern India

2020 ◽  
Vol 92 (8) ◽  
pp. 1334-1342 ◽  
Author(s):  
Mamta Chawla‐Sarkar ◽  
Anindita Banerjee ◽  
Mahadeb Lo ◽  
Suvrotoa Mitra ◽  
Keinosuke Okamoto ◽  
...  
Keyword(s):  
West Bengal ◽  
Eastern India ◽  
Human Group ◽  
Group A Rotavirus ◽  
Group A

scholarly journals Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil

2008 ◽  
Vol 103 (3) ◽  
pp. 288-294 ◽  
Author(s):  
Talissa de Moraes Tavares ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Fabíola Souza Fiaccadori ◽  
Erika Regina Leal de Freitas ◽  
Juliana Alves Parente ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Central Region ◽  
Nsp4 Gene ◽  
Human Group ◽  
The West ◽  
Group A Rotavirus ◽  
West Central ◽  
Group A

scholarly journals Experimental infection model for Sin Nombre hantavirus in the deer mouse (Peromyscus maniculatus)

2000 ◽  
Vol 97 (19) ◽  
pp. 10578-10583 ◽  
Author(s):  
J. Botten ◽  
K. Mirowsky ◽  
D. Kusewitt ◽  
M. Bharadwaj ◽  
J. Yee ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Peromyscus Maniculatus ◽  
Deer Mouse ◽  
Infection Model ◽  
Experimental Infection Model
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