scholarly journals Phytohormone Signaling of the Resistance to Plum pox virus (PPV, Sharka Disease) Induced by Almond (Prunus dulcis (Miller) Webb) Grafting to Peach (P. persica L. Batsch)

Viruses ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 238 ◽  
Author(s):  
Azam Dehkordi ◽  
Manuel Rubio ◽  
Nadali Babaeian ◽  
Alfonso Albacete ◽  
Pedro Martínez-Gómez
Keyword(s):  
Prunus Dulcis ◽  
Plum Pox Virus ◽  
Sharka Disease ◽  
Phytohormone Signaling

Causal agent of sharka disease: Plum pox virus genome and function of gene products

EPPO Bulletin ◽  
2006 ◽  
Vol 36 (2) ◽  
pp. 229-238 ◽  
Author(s):  
B. Salvador ◽  
J. A. García ◽  
C. Simón-Mateo
Keyword(s):  
Virus Genome ◽  
Causal Agent ◽  
Plum Pox Virus ◽  
Gene Products ◽  
And Function ◽  
Sharka Disease

scholarly journals First Report of Sharka Disease Caused by Plum Pox Virus in Lithuania

Plant Disease ◽  
1998 ◽  
Vol 82 (12) ◽  
pp. 1405-1405 ◽  
Author(s):  
J. Staniulis ◽  
J. Stankiene ◽  
K. Sasnauskas ◽  
A. Dargeviciute
Keyword(s):  
Coat Protein ◽  
Plant Protection ◽  
Virus Disease ◽  
Pcr Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
First Report ◽  
Das Elisa ◽  
Sharka Disease

Plum pox (sharka) disease caused by plum pox potyvirus (PPV) is considered the most important virus disease of stone fruit trees in Europe and the Mediterranean region. Nearly all those countries that produce stone fruits are affected (3). The causal virus of the disease is a European Plant Protection Organization A2 quarantine pathogen. Symptoms of leaf mottling, diffuse chlorotic spots, rings, and vein banding of varied intensity characteristic for plum pox virus infection were observed in the plum (Prunus domestica) orchard tree collection of the Lithuanian Institute of Horticulture in Babtai in 1996. Presence of this virus in the diseased trees was confirmed by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) with kits from BIOREBA (Reinach, Switzerland) and by polyclonal antibodies raised against a Moldavian isolate of PPV courtesy of T. D. Verderevskaya (Institute of Horticulture, Kishinev, Moldova). ELISAs with both sources of antiserum were positive for presence of PPV. Electron microscopy revealed the presence of potyvirus-like particles averaging 770 nm in extracts of mechanically inoculated plants of Chenopodium foetidum (chlorotic LL [local lesions]) and Pisum sativum cvs. Rainiai and Citron (mottling). For molecular diagnosis and characterization of this isolate, PPV-971, reverse transcription-polymerase chain reaction (RT-PCR) was employed. Total RNA from the leaves of infected pea was isolated as described (2). High molecular weight RNA selectively precipitated with 2 M lithium chloride was used for RT-PCR amplification of the coat protein encoding sequence by use of specific primers complementary to 5′ and 3′ parts of PPV coat protein L1 (GenBank accession no. X81081). Amino acid sequence comparison with GenBank data indicated 98.2% similarity with coat protein of PPV potyvirus isolated by E. Mais et al. (accession no. X81083) and 97.3% with PPV strain Rankovic (1).The specific DNA fragment, corresponding to predicted coat protein sequence size, was cloned into Escherichia coli pUC57 for DNA sequencing. Expression of the cloned sequence in bacteria and yeast expression systems is under investigation. The presence of PPV in plum trees in the 9-year-old collection at Babtai was confirmed by DAS-ELISA in 1997 and again in 1998. PPV was then detected in 20% of symptomatic trees of three cultivars. The Lithuanian PPV isolate reacted positively with “universal” Mab.5b and with a Mab (Mab.4DG5) specific for PPV-D. No reaction was observed with Mabs specific for PPV-M (Mab.AL), PPV-C (Mab.AC and Mab.TUV), and PPV-El Amar (Mab.EA24). PPV-971 seems to be a typical member of the less aggressive Dideron strain cluster of PPV (D. Boscia, personal communication). This is the first report of PPV in Lithuania and confirms the necessity for continuing the precautionary measures established in this country for indexing of nursery plum trees used for graft propagation. References: (1) S. Lain et al. Virus Res. 13:157, 1989. (2) J. Logemann et al. Anal. Biochem. 163:16, 1987. (3) M. Nemeth. OEPP/EPPO Bull. 24:525, 1994.

SURVEY OF SHARKA DISEASE (PLUM POX VIRUS) ON STONE FRUIT TREES IN CHILE

1998 ◽  
pp. 393-400 ◽  
Author(s):  
G. Herrera ◽  
P. Sepúlveda ◽  
M. Madariaga
Keyword(s):  
Fruit Trees ◽  
Stone Fruit ◽  
Plum Pox Virus ◽  
Sharka Disease

scholarly journals Molecular Plant-Plum Pox Virus Interactions

2020 ◽  
Vol 33 (1) ◽  
pp. 6-17 ◽  
Author(s):  
Bernardo Rodamilans ◽  
Adrián Valli ◽  
Juan Antonio García
Keyword(s):  
Life Cycle ◽  
Plant Virus ◽  
Plum Pox Virus ◽  
Comprehensive Overview ◽  
Viral Pathogens ◽  
Virion Assembly ◽  
Host Interactions ◽  
Resistant Species ◽  
Virus Interactions ◽  
Sharka Disease

Plum pox virus, the agent that causes sharka disease, is among the most important plant viral pathogens, affecting Prunus trees across the globe. The fabric of interactions that the virus is able to establish with the plant regulates its life cycle, including RNA uncoating, translation, replication, virion assembly, and movement. In addition, plant-virus interactions are strongly conditioned by host specificities, which determine infection outcomes, including resistance. This review attempts to summarize the latest knowledge regarding Plum pox virus–host interactions, giving a comprehensive overview of their relevance for viral infection and plant survival, including the latest advances in genetic engineering of resistant species.

scholarly journals Determination of Plum pox virus, the causal agent of Sharka Disease, in Samsun Province

2015 ◽  
Vol 30 (3) ◽  
pp. 227 ◽  
Author(s):  
İlyas Deligöz ◽  
Kemal Değirmenci ◽  
Miray Sökmen
Keyword(s):  
Causal Agent ◽  
Plum Pox Virus ◽  
Sharka Disease
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