scholarly journals New Approach for Mining Site Reclamation Using Alternative Substrate Based on Phosphate Industry By-Product and Sludge Mixture

2021 ◽  
Vol 13 (19) ◽  
pp. 10751
Author(s):  
Yao Kohou Donatien Guéablé ◽  
Youssef Bezrhoud ◽  
Haitam Moulay ◽  
Lhoussaine Moughli ◽  
Mohamed Hafidi ◽  
...  

Mining soils are generally characterized by soils having a coarse texture and low fertility, which makes revegetation a very difficult and delicate operation, especially in arid and semi-arid zones. The main objective of this work is to evaluate different substrates that can both contribute to the successful reclamation of phosphate mining soils and the valorization of phosphate by-product and sewage sludge. The study was carried out in pots under a greenhouse on Italian ryegrass (Lolium multiflorum). The experimental design is a randomized complete block with ten treatments, four repetitions from five substrates: phosphogypsum (PG), phosphate sludge (PS), sewage sludge (SS), topsoil from mining (TS) and phosphate waste rocks (PWR); this corresponds to soil after rock phosphate extraction. Nitrogen fertilization was applied to treatments after soil depletion in treatments not receiving sludge. An aerial biomass measurement and nutrient analysis were carried out for the three cuts. The results showed that a proportion of 65% of PG enriched the substrate in phosphorus by improving the crop yield. The addition of 5% of SS contributed to a significant improvement of ryegrass aerial biomass. In the absence of SS application, the addition of nitrogen is required to maintain crop growth. For large-scale application, TS can be mixed with PS, SS and PG for mine site reclamation.

2018 ◽  
Vol 44 (2) ◽  
pp. 219-232 ◽  
Author(s):  
S.J Jang ◽  
K.R. Kim ◽  
Y.B. Yun ◽  
S.S. Kim ◽  
Y.I Kuk

Euphytica ◽  
2021 ◽  
Vol 217 (1) ◽  
Author(s):  
Wenqing Tan ◽  
Di Zhang ◽  
Nana Yuyama ◽  
Jun Chen ◽  
Shinichi Sugita ◽  
...  

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.


1989 ◽  
Vol 264 (3) ◽  
pp. 857-862 ◽  
Author(s):  
P A Gleeson ◽  
M McNamara ◽  
R E H Wettenhall ◽  
B A Stone ◽  
G B Fincher

An arabinogalactan-protein (AGP) purified from the filtrate of liquid-suspension-cultured Italian-ryegrass (Lolium multiflorum) endosperm cells by affinity chromatography on myeloma protein J539-Sepharose was deglycosylated with trifluoromethanesulphonic acid to remove polysaccharide chains that are covalently associated with hydroxyproline residues in the peptide component of the proteoglycan. The protein core, which accounts for less than 10% (w/w) of the intact proteoglycan, was purified by h.p.l.c. It has an apparent Mr of 35,000, but reacts very poorly with both Coomassie Brilliant Blue R and silver stains. Amino-acid-sequence analysis of the N-terminus of the h.p.l.c.-purified protein core and of tryptic peptides generated from the unpurified protein reveals a high content of hydroxyproline and alanine. These are sometimes arranged in short (Ala-Hyp) repeat sequences of up to six residues. Polyclonal antibodies raised against the protein core do not cross-react with native AGP, the synthetic peptide (Ala-Hyp)4, poly-L-hydroxyproline or poly-L-proline. The results suggest that the polysaccharide chains in the native AGP render the protein core of the proteoglycan inaccessible to the antibodies and that the immunodominant epitopes include domains of the protein other than those rich in Ala-Hyp repeating units.


Author(s):  
Afonso Henrique Schaeffer ◽  
Diógenes Cecchin Silveira ◽  
Otávio Augusto Schaeffer ◽  
Nadia Canali Lângaro ◽  
Leandro Vargas

Author(s):  
Eun Chan Jeong ◽  
◽  
Hak Jin Kim ◽  
Yan Fen Li ◽  
Meing Joong Kim ◽  
...  

2001 ◽  
Vol 1 ◽  
pp. 745-749 ◽  
Author(s):  
Elizabeth Wilson ◽  
Pippa J. Chapman ◽  
Adrian McDonald

The ARBRE (ARable Biomass Renewable Energy) project, the first large-scale wood-fueled electricity generating plant in the U.K., represents a significant development in realising British and European policy objectives on renewable energy. The plant is fueled by a mix of wood from short rotation coppice (SRC) and forest residues. Where feasible, composted/conditioned sewage sludge is applied to coppice sites to increase yields and improve soil structure. In the Yorkshire Water region, typical total N:P:K composition of composted/conditioned sludge is 2.9:3.8:0.3, respectively. Sludge application is calculated on the basis of total nitrogen (N) content to achieve 750 kg N ha-1, for 3 years’ requirement. Willow coppice forms a dense, widely spaced, root network, which, with its long growing season, makes it an effective user of nutrients. This, in combination with willow’s use as a nonfood, nonfodder crop, makes it an attractive route for the recycling of sewage sludge in the absence of sea disposal, banned under the EC Urban Waste Water Treatment Directive (UWWTD). Further work is required on the nutritional requirements of SRC in order to understand better the quantities of sludge that can be applied to SRC without having a detrimental impact on the environment. This paper suggests the source of N rerouting under the UWWTD and suggests the likely expansion of SRC as an alternative recycling pathway.


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