scholarly journals Sustainable Management of Soil-Borne Bacterium Ralstonia solanacearum In Vitro and In Vivo through Fungal Metabolites of Different Trichoderma spp.

2021 ◽  
Vol 13 (3) ◽  
pp. 1491
Author(s):  
Yancui Guo ◽  
Zhenyu Fan ◽  
Xiong Yi ◽  
Yuhong Zhang ◽  
Raja Asad Ali Khan ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Bacterial Population ◽  
Sem Analysis ◽  
Wilt Disease ◽  
Fungal Metabolites ◽  
Trichoderma Spp ◽  
Tomato Plants ◽  
Bacterial Wilt Disease

The efficacy of traditional control measures for the management of plant pathogens is decreasing, and the resistance of these pathogens to pesticides is increasing, which poses a serious threat to global food security. The exploration of novel and efficient management measures to combat plant disease is an urgent need at this time. In this study, fungal metabolites from three Trichoderma spp. (T. harzianum, T. virens and T. koningii) were prepared on three different growth media (STP, MOF and supermalt (SuM)). The fungal metabolites were tested in vitro and in vivo from March–April 2020 under greenhouse conditions in a pot experiment utilizing completely randomized design to test their management of the bacterial wilt disease caused by R. solanacearum in tomato plants. The effect of the fungal metabolites on bacterial cell morphology was also investigated through scanning electron microscopy (SEM) analysis. In vitro investigation showed that the fungal metabolites of T. harzianum obtained on the STP medium were the most effective in inhibiting in vitro bacterial growth and produced a 17.6 mm growth inhibition zone. SEM analysis confirms the rupture of the cell walls and cell membranes of the bacterium, along with the leakage of its cell contents. Generally, fungal metabolites obtained on an STP medium showed higher activity than those obtained on the other two media, and these metabolites were then evaluated in vivo according to three application times (0 days before transplantation (DBT), 4 DBT and 8 DBT) in a greenhouse trial to examine their ability to manage R. solanacearum in tomato plants. Consistent with in vitro results, the results from the greenhouse studies showed a level of higher anti-bacterial activity of T. harzianum metabolites than they did for the metabolites of other fungi, while among the three application times, the longest time (8 DBT) was more effective in controlling bacterial wilt disease in tomato plants. Metabolites of T. harzianum applied at 8 DBT caused the maximum decrease in soil bacterial population (1.526 log cfu/g), resulting in the lowest level of disease severity (area under disease progressive curve (AUDPC) value: 400), and maximum plant freshness (with a resulting biomass of 36.7 g, a root length of 18.3 cm and a plant height of 33.0 cm). It can be concluded that T. harzianum metabolites obtained on an STP medium, when applied after 8 DBT, can suppress soil bacterial population and enhance plant growth, and thus can be used as a safe, environmentally-conscious and consumer-friendly approach to managing bacterial wilt disease in tomato plants and possibly other crops.

scholarly journals Biological control of bacterial wilt in tomato through the metabolites produced by the biocontrol fungus, Trichoderma harzianum

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Liu Yan ◽  
Raja Asad Ali Khan
Keyword(s):  
Plant Growth ◽  
Disease Severity ◽  
Bacterial Wilt ◽  
Trichoderma Harzianum ◽  
Bacterial Population ◽  
Inhibition Zone ◽  
Greenhouse Experiment ◽  
Fungal Metabolites ◽  
Tomato Plants

Abstract Background Ralstonia solanacearum causes bacterial wilt disease in tomato and other crops resulting in huge economic losses worldwide. Several measures have been explored for the control of R. solanacearum, but the desired control level of the disease through sustainable and ecofriendly way is still awaited. Main body In this study, fungal metabolites produced by Trichoderma harzianum were investigated in the form of crude extract for the management of R. solanacearum both in vitro and in planta in tomato plants. In in vitro investigation, fungal metabolites were checked for their antibacterial potential at different concentrations (30, 60, 90, 120, 150, and 180 mg ml−1) and bacterial cell morphology was observed under scanning electron microscopy (SEM). In a greenhouse experiment, different application times (0, 3, and 6 days before transplantation DBT) and doses (0, 3, 6, and 9%) of the fungal metabolites were tested for their effects on soil bacterial population, disease severity and plant growth of tomato plants. The in vitro evaluation showed a strong antibacterial activity of fungal metabolites in concentration dependent manner. The highest concentration 180 mg ml−1 produced maximum inhibition zone (20.2 mm) having non-significant difference with the inhibition zone (20.5 mm) produced by the standard antibiotic streptomycin. The SEM analysis revealed severe morphological destructions of bacterial cells. In case of greenhouse experiment, the highest decrease in soil bacterial population, lowest disease severity, and maximum increase in plant growth parameters were obtained by highest dose (9%) and longest application time (6 DBT). Conclusion The fungal metabolites produced by T. harzianum could be used as low-cost, environment-friendly, and sustainable management strategy for the control of R. solanacearum in tomato plants.

scholarly journals Activity test of Bacillus Spp against bacterial wilt (R. solanacearum) on tomatoes by in vitro

2021 ◽  
Vol 883 (1) ◽  
pp. 012027
Author(s):  
G N C Tuhumury ◽  
J V Hasinu ◽  
H Kesaulya
Keyword(s):  
Bacterial Wilt ◽  
Pathogenic Bacteria ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Activity Test ◽  
Resistant Varieties ◽  
Bacillus Spp ◽  
Chemical Pesticides

Abstract Ralstonia solanocearum is a pathogenic bacteria that attacks tomatoes and causes wilt disease. Many efforts have been made to control this disease through cultivation, use of chemical pesticides, and development of resistant varieties, but bacterial wilt disease remains a serious problem economically. Nowadays, many biological controls are being developed using microbes. The use of Bacillus spp as an unfriendly microbe is very potential to control because it has pathogenic inhibitory activity. This study aims to obtain bacterial isolates of Bacillus spp which can suppress the development of bacterial wilt disease in tomato plants. The research was conducted in vitro at the Laboratory of Plant Physiology in the Agriculture Faculty, Unpatti. The results showed that Bacillus niabensis strain PT-32-1 and Bacillus subtilis strain SW116b could inhibit Ralstonia solanacearum, wilt disease in tomato plants in vitro.

scholarly journals SKRINING KETAHANAN SOMAKLON NILAM TERHADAPP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum)

2016 ◽  
Vol 21 (3) ◽  
pp. 131
Author(s):  
S. Y. HARTATI ◽  
E. HADIPOENTYANTI ◽  
AMALIA AMALIA ◽  
NURSALAM NURSALAM
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Screening Method ◽  
Wilt Disease ◽  
Resistance Screening ◽  
Bacterial Wilt Disease ◽  
House Condition ◽  
Moderately Resistant

<p>ABSTRAK</p><p>Layu   bakteri  yang   disebabkan   oleh <em>  Ralstonia   solanacearum <br /> </em>merupakan salah satu penyakit penting pada tanaman nilam. Perakitan <br /> varietas nilam tahan terhadap penyakit tersebut yang dilakukan melalui <br /> induksi keragaman somaklonal telah menghasilkan beberapa somaklon <br /> yang tahan terhadap <em>R. solanacearum</em> secara <em>in-vitro</em>. Tujuan penelitian <br /> adalah menguji tingkat ketahanan somaklon tersebut terhadap penyakit <br /> layu  pada  kondisi  rumah  kaca (<em>in-vivo</em>).  Penelitian  disusun  dalam <br /> Rancangan  Acak  Lengkap  dengan 27  perlakuan, 3  ulangan,  dan 10 <br /> tanaman/ulangan. Sebagian akar dari somaklon nilam dilukai (dipotong), <br /> selanjutnya diinokulasi (disiram) dengan suspensi<em> R. solanacearum</em> dengan <br /> berbagai konsentrasi 10<sup>5</sup>, 10<sup>7</sup>, dan 10<sup>9 </sup><em>cfu</em>/ml, sebanyak 50 ml/tanaman. <br /> Hasil penelitian menunjukkan, bahwa somaklon yang diinokulasi dengan <br /> konsentrasi 10<sup>5 </sup><em>cfu</em>/ml, 50  ml/tanaman  semuanya  tidak  menunjukkan <br /> gejala layu. Somaklon yang diinokulasi dengan konsentrasi 107 dan 10<sup>9</sup></p><p><em>cfu</em>/ml,  50 ml/tanaman, sebagian layu dan mati.  Dari somaklon yang</p><p>7</p><p>diinokulasi dengan konsentrasi 10     <em>cfu</em>/ml, 50 ml/tanaman, 8 di antaranya</p><p>menunjukkan respon sangat tahan, 4 tahan, dan 5 agak tahan. Ke 17 <br /> somaklon tersebut mempunyai intensitas penyakit &lt;50% dan semua lebih <br /> tahan dari pada varietas Sidikalang (agak toleran). Dari 17 somaklon yang <br /> diinokulasi dengan konsentrasi 10<sup>9 </sup><em>cfu</em>/ml, 50 ml/tanaman, 2 di antaranya <br /> sangat tahan dan 7 somaklon tahan. Teknik skrining ini dapat digunakan <br /> sebagai  metode  standar  untuk pengujian  ketahanan  nilam  terhadap <br /> penyakit layu.</p><p>Kata kunci:  Skrining  ketahanan,  somaklon,  nilam,  penyakit  layu,<em>  R. solanacearum.</em></p><p><em> </em></p><p><em></em>ABSTRACT</p><p>Resistance-Screening of Patchouli Somaclones on Bacterial Wilt Disease (Ralstonia solanacearum) </p><p>Bacterial wilt caused by <em>Ralstonia solanacearum </em>is one of the most <br /> important  diseases  on patchouli.  The  developing patchouli resistance <br /> varieties against  wilt  disease  conducted  through the  induction  of <br /> somaclonal variation produced resistant patchouli somaclones against <em>R. <br /> </em><em>solanacearum </em>(in-vitro). The aim of this  research was to screen the <br /> resistance of those patchouli somaclones against wilt disease under a glass <br /> house condition (in-vivo). The research was conducted in a Randomized <br /> Completely Design  with 27 treatments, 3  replicates,  and 10  plants/ <br /> replicate. Some roots of the patchouli somaclones were wounded (cut), <br /> then inoculated (drenched)   with <em>  R.   solanacearum</em>  suspension   in <br /> concentration of 10<sup>5</sup>, 10<sup>7</sup>, and 10<sup>9 </sup><em>cfu</em>/ml; 50 ml/plant. The result showed, <br /> that all the patchouli somaclones inoculated with <em>R. solanacearum</em> 10<sup>5 <br /> </sup>cfu/ml, 50 ml/plant were not show any wilt sympthom. Whereas, some <br /> somaclones inoculated with the higher concentration 10<sup>7 </sup>and 109 cfu/ml, 50 ml/plant were wilted and died. Among the somaclones inoculated with  the concentration of 10<sup>7</sup>  cfu/ml, 50 ml/plant, 8 of them were highly  resistant, 4 were resistant, and 5 were moderately resistant. The disease  intencity of those 17 somaclones were &lt;50% and they were more resistant than  the  Sidikalang  variety  (moderately  tolerant).  Among  those  17 <br /> somaclones inoculated with the concentration of 10<sup>9 </sup>cfu/ml, 50 ml/plant, 2 <br /> of them were highly resistant and 7 were resistant. This screening method <br /> could be used as a standard protocol for patchouli resistance screening <br /> against wilt disease.</p><p>Kata kunci: Screening resistance, somaclone, patchouli, wilt disease, <em>R. solanacearum.</em></p>

scholarly journals Trehalose induces tomato defenses and increases drought tolerance and bacterial wilt disease resistance

2021 ◽  
Author(s):  
April M MacIntyre ◽  
Valerian Meline ◽  
Zachary Gorman ◽  
Steven P Augustine ◽  
Carolyn J Dye ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Stomatal Conductance ◽  
Water Use ◽  
Bacterial Wilt ◽  
Xylem Sap ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Infected Tomato ◽  
Use Efficiency

Ralstonia solanacearum causes plant bacterial wilt disease, leading to severe crop losses. Xylem sap from R. solanacearum-infected tomato is enriched in host produced trehalose. Water stressed plants accumulate the disaccharide trehalose, which increases drought tolerance via abscisic acid (ABA) signaling networks. Because infected plants have reduced water flow, we hypothesized that bacterial wilt physiologically mimics drought stress, which trehalose could mitigate. Transcriptomic responses of susceptible vs. resistant tomato plants to R. solanacearum infection revealed differential expression of drought-associated genes, including those involved in ABA and trehalose metabolism. ABA was enriched in xylem sap from R. solanacearum-infected plants. Treating roots with ABA lowered stomatal conductance and reduced R. solanacearum stem colonization. Treating roots with trehalose increased ABA in xylem sap and reduced plant water use by reducing stomatal conductance and temporarily improving water use efficiency. Further, trehalose-treated plants were more resistant to bacterial wilt disease. Trehalose treatment also upregulated expression of salicylic acid (SA)-dependent defense genes, increased xylem sap levels of SA and other antimicrobial compounds, and increased wilt resistance of SA-insensitive NahG tomato plants. Additionally, trehalose treatment increased xylem concentrations of jasmonic acid and related oxylipins. Together, these data show that exogenous trehalose reduced both water stress and bacterial wilt disease and triggered systemic resistance. This suite of responses revealed unexpected linkages between plant responses to biotic and abiotic stress and suggests that that R. solanacearum-infected tomato plants produce more trehalose to improve water use efficiency and increase wilt disease resistance. In turn, R. solanacearum degrades trehalose as a counter-defense.

scholarly journals Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro

2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biological Control Agent ◽  
Wilt Disease ◽  
Molecular Characteristics ◽  
Streptomyces Sp ◽  
Bacterial Wilt Disease ◽  
Wide Range

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.

scholarly journals PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN PSEUDOMONAD FLUORESEN

2020 ◽  
Vol 11 (1) ◽  
pp. 19
Author(s):  
NASRUN NASRUN ◽  
CHRISTANTI CHRISTANTI ◽  
TRIWIDODO ARWIYANTO ◽  
IKA MARISKA
Keyword(s):  
Plant Growth ◽  
Bacterial Wilt ◽  
Block Design ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Plant Production ◽  
Fluorescent Pseudomonad ◽  
Bacterial Wilt Disease ◽  
Pogostemon Cablin

<p>ABSTRAK<br />Penelitian pengendalian penyakit layu bakteri nilam (Ralstonia<br />solanacearum) menggunakan pseudomonad fluoresen di kebun petani<br />nilam Desa Situak Pasaman Barat, Sumatera Barat telah dilakukan pada<br />bulan Oktober 2003 sampai dengan Juni 2004. Penelitian ini bertujuan<br />untuk mendapatkan pseudomonad fluoresen yang berpotensi untuk<br />mengendalikan penyakit layu bakteri, dan meningkatkan pertumbuhan<br />dan produksi nilam. Isolat pseudomonad fluoresen Pf 63, Pf 90, Pf 91, Pf<br />147, dan Pf 180 sebagai perlakuan diisolasi dari rizosfer nilam sehat, dan<br />diseleksi  berdasarkan  kemampuan  antagonistik  terhadap  R.<br />solanacearum secara in vitro di Laboratorium Bakteriologi Tumbuhan<br />Fakultas Pertanian UGM. Isolat pseudomonad fluoresen tersebut<br />diintroduksikan ke nilam dan diadaptasikan selama 1 minggu sebelum<br />ditanam. Tanaman yang telah diperlakukan dengan isolat pseudomonad<br />fluoresen ditanam pada kebun yang telah terinfeksi dengan patogen pada<br />bulan Oktober 2003. Percobaan disusun dalam rancangan acak kelompok<br />(RAK) dengan 6 ulangan. Parameter pengamatan adalah masa inkubasi,<br />intensitas penyakit, pertumbuhan tanaman dan produksi minyak nilam.<br />Hasil penelitian menunjukkan bahwa isolat pseudomonad fluoresen<br />dapat mengendalikan penyakit layu bakteri dengan perpanjangan masa<br />inkubasi 6-52 hari dan penekanan intensitas penyakit 31,11 – 50,56%.<br />Disamping itu isolat pseudomonad fluoresen dapat mempengaruhi<br />peningkatan pertumbuhan tanaman yaitu tinggi tanaman (6,7 – 26,3 cm),<br />jumlah daun (4,6 – 30,1 daun/tanaman) dan berat kering daun (24,5 –<br />154,3 g/tanaman), dan produksi minyak nilam terutama jumlah minyak<br />(4,8 – 22,3 ml/tanaman). Hasil percobaan ini menunjukkan bahwa isolat<br />Pf 91 mempunyai kemampuan antagonistik tertinggi dalam mengen-<br />dalikan penyakit layu bakteri di lapangan.<br />Kata kunci : Nilam, Pogostemon cablin Benth, penyakit layu, bakteri,<br />pengendalian hayati, pseudomonad fluoresen</p><p><br />ABSTRACT<br />Controlling bacterial wilt disease on patchouli plant with<br />fluorescent pseudomonad<br />The study of controlling bacterial wilt disease on patchouli plant<br />(Ralstonia solanacearum) with fluorescent pseudomonad was carried out<br />in a farmer’s field in Situak Village West Pasaman, West Sumatera from<br />October 2003 to June 2004. The aims of the study were to find out the<br />effectiveness of fluorescent pseudomonad for controlling bacterial wilt<br />disease, increasing plant growth and production. Isolates of fluorescent<br />pseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments were<br />isolated from the rhizosphere of healthy patchouli plant, and selected<br />based on antagonistic activity on R. solanacearum in vitro at the<br />Laboratory of Plant Bacteriology, Faculty of Agriculture, UGM. The<br />isolates were inoculated on patchouli plant and adapted for one week<br />before planting. The plants treated with fluorescent pseudomonad<br />isolates were planted in the field infected with pathogen on October<br />2003. The experiment was arranged in a randomized block design<br />(RBD) with six replications. The assessment parameters were incubation<br />period, disease intensity, plant growth and production of patchouli plants.<br />The results showed that fluorescent pseudomonad isolates could control<br />the bacterial wilt disease and delay the incubation period 6-52 days and<br />decrease the disease intensity 31,11–50,56%. In addition fluorescent<br />pseudomonad isolates could affect the increase of plant growth, i.e. plant<br />height ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dry<br />weight of leaves (24,5 – 154,3 g/plant), and plant production, especially<br />oil content (4,8 – 22,3 ml/plant). The results of the experiment showed<br />that Pf 91 isolate had the highest antagonistic activity on controlling the<br />bacterial wilt disease on field.<br />Key words : Patchouli, Pogostemon cablin Benth, wilt disease,<br />bacterial, biological control, fluorescent pseudomonad</p>

scholarly journals Bacterial Tomato Pathogen Ralstonia solanacearum Invasion Modulates Rhizosphere Compounds and Facilitates the Cascade Effect of Fungal Pathogen Fusarium solani

2020 ◽  
Vol 8 (6) ◽  
pp. 806
Author(s):  
Lv Su ◽  
Lifan Zhang ◽  
Duoqian Nie ◽  
Eiko E. Kuramae ◽  
Biao Shen ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Bacterial Wilt ◽  
Fusarium Solani ◽  
Ralstonia Solanacearum ◽  
High Throughput Sequencing ◽  
Rhizosphere Soil ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Pathogen Invasion

Soil-borne pathogen invasions can significantly change the microbial communities of the host rhizosphere. However, whether bacterial Ralstonia solanacearum pathogen invasion influences the abundance of fungal pathogens remains unclear. In this study, we combined high-throughput sequencing, qPCR, liquid chromatography and soil culture experiments to analyze the rhizosphere fungal composition, co-occurrence of fungal communities, copy numbers of functional genes, contents of phenolic acids and their associations in healthy and bacterial wilt-diseased tomato plants. We found that R. solanacearum invasion increased the abundance of the soil-borne pathogen Fusarium solani. The concentrations of three phenolic acids in the rhizosphere soil of bacterial wilt-diseased tomato plants were significantly higher than those in the rhizosphere soil of healthy tomato plants. In addition, the increased concentrations of phenolic acids significantly stimulated F. solani growth in the soil. Furthermore, a simple fungal network with fewer links, nodes and hubs (highly connected nodes) was found in the diseased tomato plant rhizosphere. These results indicate that once the symptom of bacterial wilt disease is observed in tomato, the roots of the wilt-diseased tomato plants need to be removed in a timely manner to prevent the enrichment of other fungal soil-borne pathogens. These findings provide some ecological clues for the mixed co-occurrence of bacterial wilt disease and other fungal soil-borne diseases.

scholarly journals Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum)

2017 ◽  
Vol 18 (4) ◽  
pp. 1562-1567
Author(s):  
YULMIRA YANTI ◽  
TRIMURTI HABAZAR ◽  
REFLINALDON REFLINALDON ◽  
CHAINUR RAHMAN NASUTION ◽  
SRIMANO FELIA
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Subtilis Strain ◽  
Bacillus Sp ◽  
Bacterial Wilt Disease ◽  
Bacillus Spp ◽  
Growth Promoting ◽  
And Control

Yanti Y, Habazar T, Reflinaldon, Nasution CR, Felia S. 2017. Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum). Biodiversitas 18: 1562-1567. Among the species of Plant Growth Promoting Rhizobacteria, Bacillus spp. has been found to provide beneficial effects to different plant species. Based on our previous research from in planta screening’s method, we found six indigenous strains of Bacillus spp., which had the ability to control bacterial wilt and increased growth and yields of chili. Those were Bacillus subtilis BSn5,q Bacillus sp. RD-AZPVI-03, Bacillus sp. Y81-1, B.cereus strain C38/15, B.cereus strain APSB-03, and B. subtilis strain CIFT-MFB-4158A. This research aimed to characterize the mechanism of selected Bacillus spp. as biocontrol agents of R. solanacearum and as biofertilizer of chili in vitro. We have observed the biocontrol characters (production of HCN, siderophore, biosurfactant, protease, root colonization, and antibiotic), and biofertilizer’s characters (production of Indole Acetic Acid (IAA), ammonia and phosphate solubilization) of Bacillus spp. All strains had ability to produce variable concentrations of IAA, B.subtilis strain CIFT-MFB-4158A can produce siderophore, all isolates cannot produce HCN and biosurfactant, 4 isolates can produce protease. Based on the ability to colonize roots, B.subtilis strain CIFT-MFB-4158A had the best ability to colonize root surface (4.17 x 105 CFU/g root), while B. subtilis BSn5 had the best ability to colonize internal roots (2.08 x 105 CFU/g), and 2 Bacillus strains can suppress R. solanacearum in vitro.

scholarly journals Potensi Limbah Media Jamur Konsumsi untuk Menekan Penyakit Layu Bakteri (Ralstonia solanacearum) pada Tanaman Kentang

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Noor Istifadah ◽  
P. Rizky D. Sianipar
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Oyster Mushroom ◽  
Lentinus Edodes ◽  
Spent Mushroom Substrate ◽  
Organic Matters ◽  
Bacterial Wilt Disease ◽  
Auricularia Auricula

ABSTRACTThe Potential of Spent Mushroom Substrate to Control Bacterial Wilt Disease (Ralstoniasolanacearum) in PotatoBacterial wilt caused by Ralstonia solanacearum is one of important diseases in potato. One ofecofriendly-control measures is the use of organic matters including spent mushroom substrate.This paper discussed the study that examined the potential of spent substrates of oyster mushroom(Pleurotus sp.), shiitake (Lentinus edodes), Auricularia auricula and composted spent mushroomsubstrate to inhibit the growth of Ralstonia solanacearum in vitro and to suppress the diseaseincidence of bacterial wilt disease in potato. The results showed that spent substrate of shiitakemushroom slightly inhibited the growth of R. solanacearum in vitro with inhibition zone 1.5 mm.The spent mushroom substrate applied in the growth medium and drenching its water extractevery week significantly reduced the disease incidence of bacterial wilt disease, however thesuppression level was only 26.1%. The spent substrates of oyster mushroom (Pleurotus sp)., A.auricula and composted spent mushroom substrate did not suppress the disease incidence.Keywords : Lentinus edodes, Pleurotus, Auricularia auricula, organic matters, spent mushroomwater extractABSTRAKLayu bakteri yang disebabkan Ralstonia solanacearum merupakan salah satu penyakit penting padapertanaman kentang. Salah satu cara pengendalian penyakit yang ramah lingkungan adalahdengan menggunakan bahan organik, termasuk limbah media jamur konsumsi. Artikel inimendiskusikan penelitian yang mengkaji potensi limbah media tanam jamur tiram (Pleurotus sp.),jamur shiitake (Lentinus edodes), jamur kuping (Auricularia auricula) dan kompos limbah mediajamur konsumsi untuk menghambat R. solanacearum in vitro serta menekan penyakit layu bakteripada tanaman kentang. Hasil percobaan menunjukkan air rendaman limbah media jamur shiitakerelatif menekan R. solanacearum secara in vitro dengan zona penghambatan 1,5 mm. Limbahmedia jamur shiitake yang diaplikasikan pada medium tanam dan air rendamannya disiramkan ketanaman kentang setiap minggu sekali dapat menurunkan persentase tanaman layu secara nyatadibandingkan dengan kontrol, namun tingkat penekanannya hanya sebesar 26,1%. Limbah mediajamur tiram (Pleurotus sp.), jamur kuping (A. auricula) serta kompos limbah media jamur tidakmenghambat R. solanacearum secara in vitro dan tidak dapat menekan penyakit layu bakteri padatanaman kentang.Kata kunci : Lentinus edodes , Pleurotus, Auricularia auricula, bahan organik, air rendamanlimbah jamur

scholarly journals MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG

2015 ◽  
Vol 15 (1) ◽  
pp. 64 ◽  
Author(s):  
Nur Prihatiningsih ◽  
Triwidodo Arwiyanto ◽  
Bambang Hadisutrisno ◽  
Jaka Widada
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Leaf Area ◽  
Incubation Period ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Disease Index ◽  
Growth Promoter ◽  
Bacterial Wilt Disease

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.

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