scholarly journals How to Discover Traditional Varieties and Shape in a National Germplasm Collection: The Case of Finnish Seed Born Apples (Malus × domestica Borkh.)

2019 ◽  
Vol 11 (24) ◽  
pp. 7000 ◽  
Author(s):  
Maarit Heinonen ◽  
Lidija Bitz
Keyword(s):  
Malus Domestica ◽  
Germplasm Collection ◽  
Ex Situ ◽  
Malus Domestica Borkh ◽  
The North ◽  
Northern European ◽  
Program Strategy ◽  
Need To Evaluate ◽  
Good Characterization

Cultivated apple (Malus × domestica Borkh.) is a major crop of economic importance, both globally and regionally. It is currently, and was also in the past, the main commercial fruit in the northern European countries. In Finland, apple trees are grown on the frontier of their northern growing limits. Because of these limits, growing an apple tree from a seed was discovered in practice to be the most appropriate method to get trees that bear fruit for people in the north. This created a unique culturally and genetically rich native germplasm to meet the various needs of apple growers and consumers from the late 1800s to the mid-1900s. The preservation, study and use of this genetic heritage falls within the mandate of the Finnish National Genetic Resources Program. The first national apple clonal collection for germplasm preservation was reorganized from the collections of apple breeders. The need to evaluate the accessions, both in this collection and possible missing ones, to meet the program strategy lead us to evaluate the Finnish apple heritage that is still available in situ in gardens. In this article we use multiple-approach methodologies and datasets to gain well-described, proof-rich samples for the trueness-to-type analysis of old heirloom apple varieties. The approach includes a combination of socio-historic, pomological and genotyping methods and datasets that are all valued as equally important. The main finding was that in addition to the pomological, molecular and genetic evaluation of ex situ apple collections, an extensive historical data and socio-economic conditions research are essential to perform good characterization of accessions. After implementing the results in re-creating the Finnish national apple germplasm collection, the number of Finnish local varieties was more than doubled from 38 accessions to 97.

scholarly journals Status of the Ex Situ and In Situ Conservation of Brazilian Crop Wild Relatives of Rice, Potato, Sweet Potato, and Finger Millet: Filling the Gaps of Germplasm Collections

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  
Keyword(s):  
Finger Millet ◽  
Ex Situ Conservation ◽  
Gap Analysis ◽  
In Situ Conservation ◽  
Germplasm Collection ◽  
Crop Wild Relatives ◽  
Wild Relatives ◽  
Ex Situ ◽  
The Status

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.

The waterbodies of the Dallol volcano: A physico-chemical and geo-microbial survey

2020 ◽  
Author(s):  
Hugo Moors ◽  
Miroslav Honty ◽  
Carla Smolders ◽  
Ann Provoost ◽  
Mieke De Craen ◽  
...  
Keyword(s):  
Saline Water ◽  
Halophilic Archaea ◽  
Mining Area ◽  
Ex Situ ◽  
Base Region ◽  
The South ◽  
North East ◽  
The North ◽  
Physico Chemical

<p>The geological extreme Dallol region, located around the Dallol volcano in the north-east of Danakil depression (Ethiopia), is considered as one of the harshest and hottest places on Earth. The geology is made up of years and years of evaporates accumulation. Volcanic activity generates ascending brines that may cross and mix with aquifers from inflowing meteoric water originating from the Ethiopian highlands on the east of the Danakil depression. When these mixtures reach the surface they can generate hydrothermal springs giving rise to waterbodies in the form of small ponds or lakes. During the Europlanet 2018 Danakil field expedition, ten of these saline waterbodies were extensively studied by <em>in situ</em> measurements and <em>ex situ</em> geo–physico-chemical and –microbiological analyses of collected samples, liquids as well as sediments.</p><p>The <em>in situ</em> physico-chemical measurements clearly indicated the extreme nature of all ten investigated lakes. Laboratory analyses of the collected batch samples of liquids and sediments confirmed the extreme character of the waterbodies and complements our geological survey of the region with valuable geo–chemical and –microbiological data.</p><p>Based on our analytical results, the relative small Dallol region can still be subdivided into three geological smaller areas: the outcrop zone, the volcanic base region and the distant south area. The outcrop zone is dominated by sodium, iron and potassium. Oxidation processes in the outflowing superheated ferrous and sulfidic rich brine give rise to some of the most acidic ponds on our planet. In the ponds and lakes of the volcanic base region, incredible high amounts of calcium and/or magnesium can remain in their dissolved form as the most dominant and quasi only available anion is chloride. This region is host for the most saline water body on Earth. Chemical analysis of the lakes of the distant south area show that sodium is by far the most dominant cation. It is therefore no surprise that the large Karum Lake in the south region is economically exploited for the mining of sodium chloride.</p><p>Our mineralogy analyses render results that are completely in line with the observed geochemistry of the waterbodies. Halite and sylvite are the most present minerals in the Dallol outcrop zone associated with some gypsum and in one case with anhydrite. The geology around the waterbodies of volcanic base zone are a little bit more divers. On the shores of the Gaet’ale Pond tachyhydrite, chloromagnesite, halite and sylvite is determined, while the Black Lake is surrounded by bischofite and carnalite. Logically, the mineralogy of the south area, the salt mining area, is dominated by halite and sylvite.</p><p>Apparently, the geochemistry of the outcrop zone and volcanic base region is so harsh that no extremophilic organism is able to survive in these areas. Only in the distant south area did we find indications of the presence of halophiles. Besides the bacterial genus <em>Salinibacter</em>, our 16S rDNA microbiological fingerprinting indicates the presence of halophilic archaea like:  <em>Halobaculum sp., Halobellus sp., Halomicroarcula sp., Halorientalis sp.</em> with the majority of the population being <em>Candidatus Nanosalina sp</em>.</p>

Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 108-114 ◽  
Author(s):  
José Miguel Soriano ◽  
Carlos Romero ◽  
Santiago Vilanova ◽  
Gerardo Llácer ◽  
María Luisa Badenes
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Malus Domestica ◽  
Genetic Relationships ◽  
Germplasm Collection ◽  
Mean Value ◽  
Eriobotrya Japonica ◽  
Fixation Index ◽  
Group Method ◽  
Malus Domestica Borkh

Genetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty primer pairs flanking microsatellites previously identified in Malus × domestica (Borkh.) were assayed. Thirteen of them amplified polymorphic products and unambiguously distinguished 34 genotypes from the 40 accessions analyzed. Six accessions showing identical marker patterns were Spanish local varieties thought to have been derived from 'Algerie' by a mutational process very common in loquat species. A total of 39 alleles were detected in the population studied, with a mean value of 2.4 alleles per locus. The expected and observed heterozygosities were 0.46 and 51% on average, respectively, leading to a negative value of the Wright's fixation index (–0.20). The values of these parameters indicate a smaller degree of genetic diversity in the set of loquat accessions analyzed than in other members of the Rosaceae family. Unweighted pair-group method (UPGMA) cluster analysis, based on Nei's genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. The high number of alleles and the high expected heterozygosity detected with SSR markers developed in Malus × domestica (Borkh.) make them a suitable tool for loquat cultivar identification, confirming microsatellite marker transportability among genera in the Rosaceae family.Key words: Eriobotrya japonica, SSR markers, microsatellites, genetic diversity.

Phylogenetic in situ/ex situ analysis of a sulfur mat microbial community from a thermal sulfide spring in the north Caucasus

Microbiology ◽  
2008 ◽  
Vol 77 (2) ◽  
pp. 219-223 ◽  
Author(s):  
E. Yu. Chernousova ◽  
V. N. Akimov ◽  
E. V. Gridneva ◽  
G. A. Dubinina ◽  
M. Yu. Grabovich
Keyword(s):  
Microbial Community ◽  
Ex Situ ◽  
North Caucasus ◽  
The North

scholarly journals Utilization of Identified Simple Sequence Repeats (SSRs) in Malus ×domestica (Apple) for Germplasm Characterization

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 619b-619 ◽  
Author(s):  
Amy K. Szewc-McFadden ◽  
Warren F. Lamboy ◽  
James R. McFerson
Keyword(s):  
Germplasm Collection ◽  
Ex Situ ◽  
Dna Library ◽  
The Core ◽  
Core Subset ◽  
Ssr Loci ◽  
Dna Fragment ◽  
Simple Sequence

To comprehend genetic identity and relatedness in Malus germplasm held in situ and ex situ, we are employing simple sequence repeat (SSR) DNA fragment information in combination with passport and horticultural data. SSRs offer certain advantages for characterizing large arrays of germplasm efficiently. They are abundantly dispersed throughout plant genomes and are exceedingly polymorphic. In addition, they can be PCR-amplified and detected by automated fluorescence-based technology. A size-fractionated DNA library of M. ×domestica cv Golden Delicious was screened to identify SSR loci. Eight loci were found to be reliably informative and were used to prepare locus-specific primer pairs. Characterization of the 75 M. ×domestica accessions included in the core subset of the USDA-ARS Malus germplasm collection revealed six of the eight loci were polymorphic within the array. The number of alleles per locus ranged from two to 21. Throughput was enhanced by multiplexing, allowing simultaneous use of two or three primer pairs. With improved genetic characterization of Malus germplasm, we intend to better develop and relate the core subset to the rest of the collection and to in situ Malus genetic resources. SSR markers appear to be an efficient and reliable tool to expedite this process.

Cotton Genetic Resources in the Western States of Mexico: In situ Conservation Status and Germplasm Collection for ex situ Preservation

2006 ◽  
Vol 53 (4) ◽  
pp. 653-668 ◽  
Author(s):  
Mauricio Ulloa ◽  
James McD. Stewart ◽  
Enrique A. Garcia-C. ◽  
Salvador Godoy-A. ◽  
Arturo Gaytan-M. ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Conservation Status ◽  
In Situ Conservation ◽  
Germplasm Collection ◽  
Ex Situ

scholarly journals GROWTH AND FRUITING OF 'DELICIOUS' APPLE ON CLONAL ROOTSTOCKS IN THE 1984 NC-140 PLANTING IN MAINE

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1162e-1162
Author(s):  
James R. Schupp
Keyword(s):  
New England ◽  
Malus Domestica ◽  
North Central ◽  
Malus Domestica Borkh ◽  
The Third ◽  
The North ◽  
Cooperative Testing ◽  
North Central Regional ◽  
Root Suckering ◽  
Cooperative Project

In 1984 trees of `Starkspur Supreme Delicious' apple (Malus domestica, Borkh) on 16 rootstocks were planted at 32 sites In Morth America according to guidelines established for cooperative testing by the North Central Regional Cooperative Project (NC--140). Tree loss and root suckering in the Maine planting have been low, similar to that of other sites. Tree size in Maine is smallest amoung all sites after eight seasons. Trees on Budagovsky 9 (B.9) rootstock were the most precocious, producing significantly higher flower numbers and yield in the third year. Other precocious rootstocks in this planting included C.6, M.26EMLA, M.7EMLA and P.l. After eight years, B.9, C.6 and M.26EMLA were the most productive amoung the dwarf trees. P.l and M.7EMLA were the most productive amoung the more vigorous stocks. Heavy croping trees on dwarf rootstocks leaned more due to hurricane winds than larger better anchored trees which lost a larger proportion of their crop. B.9, C.6 and P.1 may have potential as rootstocks for commercial apple orchards in New England.

scholarly journals In situ and genetic characterization of Gossypium barbadense populations from the states of Pará and Amapá, Brazil

2009 ◽  
Vol 44 (7) ◽  
pp. 719-725 ◽  
Author(s):  
Vanessa Cavalcante de Almeida ◽  
Lúcia Vieira Hoffmann ◽  
Gilberto Ken It Yokomizo ◽  
Joaquim Nunes da Costa ◽  
Marc Giband ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Germplasm Collection ◽  
Gossypium Barbadense ◽  
Ex Situ ◽  
In Situ Characterization ◽  
Total Genetic Diversity ◽  
High Level ◽  
Single Sequence

The objective of this work was to characterize the populations of Gossypium barbadense in the states of Amapá and Pará, Brazil. In situ characterization was conducted through interviews with the owners of the plants and environmental observations. Leaf or petal tissue as well as seed samples were collected for genetic characterization by single sequence repeats markers and for storage in germplasm banks, respectively. The plants were maintained in dooryards and used mainly for medical purposes. The genetic analysis showed no heterozygous plants at the loci tested (f = 1), indicating that reproduction occurs mainly through selfing. The total genetic diversity was high (He = 0.39); and a high level of differentiation was observed between cotton plants from the two states (F ST = 0.36). Conventional methods of in situ maintenance of G. barbadense populations are not applicable. The conservation of the genetic variability of populations present in the two states could be achieved through germplasm collection and establishing of ex situ seed banks.

Expression patterns of several floral genes during flower initiation in the apical buds of apple (Malus × domestica Borkh.) revealed by in situ hybridization

2011 ◽  
Vol 30 (8) ◽  
pp. 1485-1492 ◽  
Author(s):  
Naozumi Mimida ◽  
Ayano Ureshino ◽  
Norimitsu Tanaka ◽  
Narumi Shigeta ◽  
Noriko Sato ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Malus Domestica ◽  
Expression Patterns ◽  
Flower Initiation ◽  
Malus Domestica Borkh ◽  
Apical Buds
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