scholarly journals Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste

2018 ◽  
Vol 10 (7) ◽  
pp. 2433 ◽  
Author(s):  
Laura Mejias ◽  
Alejandra Cerda ◽  
Raquel Barrena ◽  
Teresa Gea ◽  
Antoni Sánchez
Keyword(s):  
Solid State ◽  
Enzymatic Activity ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Batch Reactor ◽  
Hydrolytic Enzymes ◽  
Cellulase Production ◽  
Degree Of Hydrolysis ◽  
Xylanase Production ◽  
Specific Oxygen Uptake Rate

Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.

scholarly journals Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch

2018 ◽  
Vol 13 (3) ◽  
pp. 553
Author(s):  
Vita Wonoputri ◽  
Subiantoro Subiantoro ◽  
Made Tri Ari Penia Kresnowati ◽  
Ronny Purwadi
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Contact Method ◽  
Empty Fruit Bunch ◽  
Liquid Ratio ◽  
Fermentation Parameters

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reservedReceived: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559 

Cellulase Production under Solid State Fermentation in Cashew Apple Bagasse by Trichoderma reesei LCB 48

2015 ◽  
Vol 365 ◽  
pp. 323-328
Author(s):  
Simone Aparecida da Silva Lins ◽  
Líbia de Sousa Conrado
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Initial Moisture Content ◽  
Cellulase Production ◽  
Cashew Apple Bagasse ◽  
Production Values ◽  
Initial Substrate ◽  
Substrate Moisture

Cellulases, among many enzymes, have been highlighted in several areas of expertise, such as food, textiles, pulp and paper and wastewater treatment of effluents and residues. There is also the challenge of producing biofuels, where currently cellulases have been widely applied in the production of cellulosic ethanol, where it is used during the stage of hydrolysis of lignocellulosic biomass for conversion of cellulose to glucose. Studies have been developed in order to produce this enzyme through a process of solid state fermentation from lignocellulosic agroindustrial wastes, thus reducing the cost of enzyme production, and adding value to the residue. The aim of this work was to produce cellulases from the stalk of the cashew bagasse using Trichoderma reesei LCB 48. The study of the cellulase production was performed using 22 factorial design with central point in quadruplicate. The washed stalk of the cashew bagasse inoculated with T. reesei was evaluated for the production of cellulases with initial moisture contents of 45, 55 and 65% and in the presence of inorganic nitrogen ((NH4)2SO4) at concentrations 0.5, 0.75 and 1%. The fermentation was developed over 238 hours, and during this period the parameters analyzed were pH, moisture, AR and enzyme activity expressed in CMCase. Peak production of cellulase enzyme expressed in CMCase was achieved with 238 hours which value was 0.71 U/g (0.095 U/mL), under the conditions of 45% initial moisture content and 1% of nitrogen source. This activity was obtained in only one stage of the biotechnological process, the solid state fermentation; the next ones are concentration and purification. The using of experimental design methodology allowed us to observe the initial substrate moisture is the determining variable in the production of enzymes CMCases, and the minimum moisture level (45%) showed the highest production values of CMCase.

scholarly journals Optimization of the simultaneous production of cellulase and xylanase by submerged and solid-state fermentation of wheat chaff

2020 ◽  
Vol 85 (2) ◽  
pp. 177-189 ◽  
Author(s):  
Mirjana Jovanovic ◽  
Damjan Vucurovic ◽  
Bojana Bajic ◽  
Sinisa Dodic ◽  
Vanja Vlajkov ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Submerged Fermentation ◽  
Ph Value ◽  
Agricultural Waste ◽  
Hydrolytic Enzymes ◽  
Raw Material ◽  
Optimal Conditions ◽  
Xylanase Production ◽  
Wheat Chaff

Wheat chaff as an agricultural waste represents a cheap raw material for biotechnological processes. With its lignocellulosic composition, it is suitable for producing hydrolytic enzymes for second generation renewable fuel production technologies. The aim of this work was to optimize the process parameters (cultivation temperature 25?35?C, pH value 4?6 and cultivation time 3?7 days) of the cultivating fungi (Trichoderma reesei QM 9414) on a media based on wheat chaff by submerged and solid-state techniques, in order to enhance and compare the two types of simultaneous cellulase and xylanase production. Optimal conditions for the submerged fermentation were 29.65?C for temperature, pH 4.27 and 7 days of cultivation, while for the solid-state fermentation, the optimal conditions were 28.01?C, pH 6.00 and 7 days. The cellulolytic and xylanolytic activities of the obtained cultivation broth filtrates were 0.0535 and 0.1676 U mL-1 for the submerged fermentation, and 0.0407 and 0.1401 U mL-1 for the solid-state fermentation, respectively, and with a 26.77 and 13.39 % enhancement of enzyme activity for submerged fermentation, and a 22.96 and 42.66 % enhancement for solid-state fermentation, respectively, compared to the results obtained before optimization.

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