scholarly journals Chemical and Antioxidant Characterization of the Portuguese Heather Honey from Calluna vulgaris

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 177
Author(s):  
Luís Rodrigues da Silva ◽  
Renan Campos Chisté ◽  
Eduarda Fernandes
Keyword(s):  
Oxidative Damage ◽  
Reactive Nitrogen Species ◽  
Nutritional Value ◽  
Total Content ◽  
Calluna Vulgaris ◽  
Nitrogen Species ◽  
Hemoglobin Oxidation ◽  
Scavenging Capacity ◽  
First Time

The Calluna vulgaris honey produced in Portugal, concerning its phenolic compounds and abscisic acids profiles, as well as its antioxidant activity and the protective effect against oxidative damage in human erythrocytes were herein performed for the first time. The phenolic and abscisic acid profiles were tentatively identified by LC-MS/MS (17 compounds). The total content of phenolics and abscisic acids was 15,446.4 µg/g of honey extract, with catechin derivatives and abscisic acids being major constituents. The highest scavenging capacity was found against reactive nitrogen species. Additionally, the honey extract prevented ROO•-induced oxidative damage in erythrocytes collected from human blood, by inhibiting hemolysis, lipid peroxidation and hemoglobin oxidation. In conclusion, C. vulgaris honey contains high content of catechin derivatives and abscisic acids that may be responsible for its biological activity, characterized by a strong antioxidant capacity, which adds up to the nutritional value of this delicacy.

scholarly journals Mitochondrial antioxidative capacity regulates muscle glucose uptake in the conscious mouse: effect of exercise and diet

2012 ◽  
Vol 113 (8) ◽  
pp. 1173-1183 ◽  
Author(s):  
Li Kang ◽  
Mary E. Lustig ◽  
Jeffrey S. Bonner ◽  
Robert S. Lee-Young ◽  
Wesley H. Mayes ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Reactive Nitrogen Species ◽  
Oxygen Species ◽  
Oxidized Glutathione ◽  
Nitrogen Species ◽  
Antioxidative Capacity ◽  
Mitochondrial Reactive Oxygen Species ◽  
High Fat ◽  
Nitrative Stress ◽  
Scavenging Capacity

The objective of this study was to test the hypothesis that exercise-stimulated muscle glucose uptake (MGU) is augmented by increasing mitochondrial reactive oxygen species (mtROS) scavenging capacity. This hypothesis was tested in genetically altered mice fed chow or a high-fat (HF) diet that accelerates mtROS formation. Mice overexpressing SOD2 ( sod2Tg), mitochondria-targeted catalase ( mcatTg), and combined SOD2 and mCAT (mtAO) were used to increase mtROS scavenging. mtROS was assessed by the H2O2 emitting potential ( JH2O2) in muscle fibers. sod2Tg did not decrease JH2O2 in chow-fed mice, but decreased JH2O2 in HF-fed mice. mcatTg and mtAO decreased JH2O2 in both chow- and HF-fed mice. In parallel, the ratio of reduced to oxidized glutathione (GSH/GSSG) was unaltered in sod2Tg in chow-fed mice, but was increased in HF-fed sod2Tg and both chow- and HF-fed mcatTg and mtAO. Nitrotyrosine, a marker of NO-dependent, reactive nitrogen species (RNS)-induced nitrative stress, was decreased in both chow- and HF-fed sod2Tg, mcatTg, and mtAO mice. This effect was not changed with exercise. Kg, an index of MGU was assessed using 2-[14C]-deoxyglucose during exercise. In chow-fed mice, sod2Tg, mcatTg, and mtAO increased exercise Kg compared with wild types. Exercise Kg was also augmented in HF-fed sod2Tg and mcatTg mice but unchanged in HF-fed mtAO mice. In conclusion, mtROS scavenging is a key regulator of exercise-mediated MGU and this regulation depends on nutritional state.

Characterization of reactive nitrogen species in allergic asthma

2014 ◽  
Vol 112 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Li Zuo ◽  
Majid S. Koozechian ◽  
Lauren L. Chen
Keyword(s):  
Allergic Asthma ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species

scholarly journals Eosinophils Are a Major Source of Nitric Oxide-Derived Oxidants in Severe Asthma: Characterization of Pathways Available to Eosinophils for Generating Reactive Nitrogen Species

2001 ◽  
Vol 166 (9) ◽  
pp. 5763-5772 ◽  
Author(s):  
Jennifer C. MacPherson ◽  
Suzy A. A. Comhair ◽  
Serpil C. Erzurum ◽  
Dennis F. Klein ◽  
Mary F. Lipscomb ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Severe Asthma ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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