scholarly journals Survey on Antibiotic Residues in Egg Samples in Italy

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 148
Author(s):  
Giorgio Saluti ◽  
Maria Novella Colagrande ◽  
Federica Castellani ◽  
Matteo Ricci ◽  
Gianfranco Diletti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antibiotic Resistance ◽  
Antimicrobial Agents ◽  
Current Issue ◽  
High Resolution Mass Spectrometry ◽  
Antibiotic Residues ◽  
Antibiotic Residue ◽  
A Current ◽  
Resolution Mass

The presence of antibiotic residue in eggs is a current issue due to the increasingly important phenomenon of antibiotic resistance. A multiclass, confirmatory method for the determination of seventy-three antimicrobial agents (amphenicols, cephalosporins, diaminopyrimidines, lincosamides, macrolides, penicillins, pleuromutilins, quinolones, sulfonamides, and tetracyclines) with liquid chromatography high-resolution mass spectrometry was applied to 200 egg samples collected from 119 Italian farms during the years 2018–2021.

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

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