scholarly journals Antitumor Potential of Green Synthesized ZnONPs Using Root Extract of Withania somnifera against Human Breast Cancer Cell Line

Separations ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 8
Author(s):  
Kollur Shiva Prasad ◽  
Shashanka K Prasad ◽  
Ravindra Veerapur ◽  
Ghada Lamraoui ◽  
Ashwini Prasad ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Line ◽  
Withania Somnifera ◽  
Root Extract ◽  
Dependent Manner ◽  
Sem Images ◽  
Xrd Pattern ◽  
Transmission Electron

Herein we report the synthesis of zinc oxide nanoparticles (ZnONPs) using Withania somnifera root extract (WSE) as an effective chelating agent. The microscopic techniques viz., X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED) were employed to analyze the as-obtained ZnONPs. The crystalline planes observed from the XRD pattern agrees with the hexagonal wurtzite structure of the as-prepared ZnONPs. The aggregations and agglomerations observed in the SEM images indicated that the size of the as-prepared ZnONPs was between 30 and 43 nm. The interplanar distance between the lattice fringes observed in the HRTEM image was found to be 0.253 nm, which is in good agreement with the (100) plane obtained in the XRD pattern. Furthermore, the anti-breast cancer cytotoxic evaluation was carried out using the MCF-7 cell line, and the results showed significant cytotoxic effects in a dose-dependent manner.

scholarly journals Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 385
Author(s):  
Shiva Prasad Kollur ◽  
Shashanka K. Prasad ◽  
Sushma Pradeep ◽  
Ravindra Veerapur ◽  
Sharanagouda S. Patil ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cancer Cell Line ◽  
Sem Images ◽  
Selected Area Electron Diffraction ◽  
Transmission Electron ◽  
Diffraction Patterns ◽  
Lattice Planes ◽  
Cancer Activity

The present work describes a facile and convenient procedure for synthesizing zinc oxide nanoparticles using luteolin isolated from Eclipta alba plant (L-ZnONPs) at room temperature. The formation of as-grown L-ZnONPs was confirmed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The Wurtzite structure of ZnO was observed by its hexagonal phases in diffraction patterns. The SEM images revealed the different sizes and morphologies of L-ZnONPs, with diameters between 12 and 25 nm. The HR-TEM result showed that the inter-planar distance between two lattice fringes was 0.262 nm, which coincides with the d-spacing of (002) and (101) lattice planes of the as-obtained material. The anticancer activity of L-ZnONPs against the breast cancer cell line MCF-7 was greater as compared to that of luteolin or ZnO alone. The mechanistic evaluation of such an activity carried out using in silico methods suggested that the anti-breast cancer activity of L-ZnONPs was mediated by polo-like kinase 1 (PLK1) proteins.

Hydrothermal Synthesis and Characterization of BiFeO3 Polyhedral Crystallites

2012 ◽  
Vol 174-177 ◽  
pp. 508-511
Author(s):  
Lin Lin Yang ◽  
Yong Gang Wang ◽  
Yu Jiang Wang ◽  
Xiao Feng Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Synthesis And Characterization ◽  
X Ray Diffraction ◽  
Xrd Pattern ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

BiFeO3 polyhedrons had been successfully synthesized via a hydrothermal method. The as-prepared products were characterized by power X-ray diffraction (XRD) pattern, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The possible mechanisms for the formation of BiFeO3 polyhedrons were discussed. Though comparison experiments, it was found that the kind of precursor played a key role on the morphology control of BiFeO3 crystals.

scholarly journals Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 982 ◽  
Author(s):  
Kollur Shiva Prasad ◽  
Shashanka K. Prasad ◽  
Mohammad Azam Ansari ◽  
Mohammad A. Alzohairy ◽  
Mohammad N. Alomary ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fluorescence Emission ◽  
Fluorescence Emission Spectrum ◽  
X Ray Diffraction ◽  
Sem Images ◽  
X Ray ◽  
Cassia Auriculata ◽  
Transmission Electron ◽  
Normal Human Breast

In this work, we aimed to synthesize zinc oxide nanoparticles (ZnONPs) using an aqueous extract of Cassia auriculata leaves (CAE) at room temperature without the provision of additional surfactants or capping agents. The formation of as-obtained ZnONPs was analyzed by UV–visible (ultraviolet) absorption and emission spectroscopy, X-ray photoemission spectroscopy (XPS), X-ray diffraction analysis (XRD), energy dispersive X-ray diffraction (EDX), thermogravimetric analysis/differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED). The XRD results reflect the wurtzite structure of as-prepared ZnONPs, which produced diffraction patterns showing hexagonal phases. The SEM images indicate that the morphology of as-prepared ZnONPs is composed of hexagonal nanostructures with an average diameter of 20 nm. The HR-TEM result shows that the inter-planar distance between two lattice fringes is 0.260 nm, which coincides with the distance between the adjacent (d-spacing) of the (002) lattice plane of ZnO. The fluorescence emission spectrum of ZnONPs dispersed in ethanol shows an emission maximum at 569 nm, revealing the semiconductor nature of ZnO. As-obtained ZnONPs enhanced the tumoricidal property of CAE in MCF-7 breast cancer cells without significant inhibition of normal human breast cells, MCF-12A. Furthermore, we have studied the antibacterial effects of ZnONPs, which showed direct cell surface contact, resulting in the disturbance of bacterial cell integrity.

Oxyhemoglobin-induced apoptosis in cultured endothelial cells

1999 ◽  
Vol 91 (3) ◽  
pp. 459-465 ◽  
Author(s):  
Kotaro Ogihara ◽  
Alexander Y. Zubkov ◽  
David H. Bernanke ◽  
Adam I. Lewis ◽  
Andrew D. Parent ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endothelial Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Content Type ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Transmission Electron ◽  
Fine Print

Object. Oxyhemoglobin (OxyHb) is one of the most important spasmogens for cerebral vasospasm that follows aneurysmal subarachnoid hemorrhage. The cytotoxic effect of OxyHb has been documented in endothelial and smooth-muscle cells; however, the pattern of cell death—necrosis or apoptosis—as the final stage of cell damage has not been demonstrated. This study was undertaken to determine if OxyHb induces apoptotic changes in cultured bovine aortic endothelial cells.Methods. Confluent bovine aortic endothelial cells were treated with OxyHb in a concentration- and time-dependent manner. Cell density was assayed by counting the number of cells that attached to culture dishes after exposure to OxyHb. To identify apoptotic changes, the investigators used three specific methods: DNA fragmentation (electrophoreses), the apoptotic body (transmission electron microscopy), and cleavage of poly (adenosine diphosphate ribose) polymerase (PARP [Western blotting]).Conclusions. Oxyhemoglobin decreased cell density in a concentration- and time-dependent manner. Analysis of DNA showed a pattern of internucleosomal cleavage characteristic of apoptosis (DNA ladder). Transmission electron microscopy demonstrated condensation of nuclei and apoptotic bodies in OxyHb-treated endothelial cells. Western blotting with the PARP antibody revealed that the 116-kD PARP was cleaved to the 85-kD apoptosis-related fragment. These results for the first time demonstrated that the OxyHb induces apoptosis in cultured endothelial cells.

scholarly journals Evolution of Ag nanostructures created from thin films: UV–vis absorption and its theoretical predictions

2020 ◽  
Vol 11 ◽  
pp. 494-507
Author(s):  
Robert Kozioł ◽  
Marcin Łapiński ◽  
Paweł Syty ◽  
Damian Koszelow ◽  
Wojciech Sadowski ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Layer Thickness ◽  
Photoelectron Spectroscopy ◽  
Plasmonic Nanostructures ◽  
Sem Images ◽  
Xps Spectra ◽  
Initial Layer ◽  
Novel Approach ◽  
Transmission Electron

Ag-based plasmonic nanostructures were manufactured by thermal annealing of thin metallic films. Structure and morphology were studied using scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS). SEM images show that the formation of nanostructures is influenced by the initial layer thickness as well as the temperature and the time of annealing. The Ag 3d and Ag 4d XPS spectra are characteristic of nanostructures. The quality of the nanostructures, in terms of their use as plasmonic platforms, is reflected in the UV–vis absorption spectra. The absorption spectrum is dominated by a maximum in the range of 450–500 nm associated with the plasmon resonance. As the initial layer thickness increases, an additional peak appears around 350 nm, which probably corresponds to the quadrupole resonance. For calculations leading to a better illustration of absorption, scattering and overall absorption of light in Ag nanoparticles, the Mie theory is employed. Absorbance and the distribution of the electromagnetic field around the nanostructures are calculated by finite-difference time-domain (FDTD) simulations. For calculations a novel approach based on modelling the whole sample with a realistic shape of the nanoparticles, instead of full spheres, was used. This led to a very good agreement with the experiment.

scholarly journals Life cycle ofRickettsia slovacain L929 cell line studied by quantitative real-time PCR and transmission electron microscopy

2009 ◽  
Vol 293 (1) ◽  
pp. 102-106 ◽  
Author(s):  
Vojtech BoldiÅ¡ ◽  
Jasna Å trus ◽  
Elena Kocianová ◽  
Magda TuÅ¡ek-Žnidarič ◽  
Katarína Å tefanidesová ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Life Cycle ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
L929 Cell ◽  
Quantitative Real Time Pcr ◽  
Transmission Electron ◽  
L929 Cell Line

Carbonate and cation substitutions in hydroxylapatite in breast cancer micro-calcifications

2021 ◽  
pp. 1-11
Author(s):  
Yan Zhang ◽  
Changqiu Wang ◽  
Yan Li ◽  
Anhuai Lu ◽  
Fanlu Meng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fourier Transform ◽  
Synchrotron Radiation ◽  
Optical Microscopy ◽  
Fourier Transform Infrared ◽  
X Ray ◽  
Transmission Electron ◽  
Cation Substitutions

Abstract Calcification within breast cancer is a diagnostically significant radiological feature that generally consists of hydroxylapatite. Samples from 30 cases of breast carcinoma with calcification were investigated using optical microscopy, energy-dispersive X-ray analysis, transmission-electron microscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, synchrotron radiation X-ray diffraction and X-ray fluorescence. Under optical microscopy, the calcifications were found to consist of either irregular aggregates with widths > 200 μm or spherical aggregates similar to psammoma bodies with an average diameter of 30 μm. Transmission-electron microscopy showed that short columnar or dumbbell-shaped crystals with widths of 10–15 nm and lengths of 20–50 nm were the most common morphology; spherical aggregates (~1 μm in diameter) with amorphous coatings of fibrous nanocrystals were also observed. Results indicated that hydroxylapatite was the dominant mineral phase in the calcifications, and both CO32– and cation substitutions (Na, Mg, Zn, Fe, Sr, Cu and Mn) were present in the hydroxylapatite structure. Fourier-transform infrared spectra show peaks at 872 and 880 cm–1 indicating that CO32– substituted both the OH– (A type) and PO43– (B type) sites of hydroxylapatite, making it an A and B mixed type. The ratio of B- to A-type substitution was estimated in the range of 1.1–18.7 from the ratio of peak intensities (I872/I880), accompanied with CO32– contents from 1.1% to 14.5%. Trace arsenic, detected in situ by synchrotron radiation X-ray fluorescence was found to be distributed uniformly in the calcifications in the form of AsO43– substituting for PO43–. It is therefore proposed that identifying these trace elements in breast cancer calcifications may be promising for future clinical diagnostics.

scholarly journals The Environmental Pathogen Mycobacterium ulcerans Grows in Amphibian Cells at Low Temperatures

2002 ◽  
Vol 68 (12) ◽  
pp. 6403-6404 ◽  
Author(s):  
Michel Drancourt ◽  
Vincent Jarlier ◽  
Didier Raoult
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Line ◽  
Quantitative Pcr ◽  
Low Temperatures ◽  
Mycobacterium Ulcerans ◽  
Etiological Agent ◽  
Pcr Analysis ◽  
Environmental Niche ◽  
Transmission Electron

ABSTRACT Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28°C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy demonstrated the presence of intracellular M. ulcerans microorganisms. These data suggest an intracellular environmental niche, and we propose use of XTC-2 cells for isolation of M. ulcerans from environmental sources.

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