scholarly journals Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

Separations ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 8 ◽  
Author(s):  
Marimuthu alias Antonysamy Johnson ◽  
Janarthanan Gowtham ◽  
Narayanan Janakiraman ◽  
Tharmaraj Renisheya Joy Jeba Malar ◽  
Janaina E. Rocha ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Methanolic Extracts ◽  
Different Types ◽  
Crude Drugs ◽  
Phytochemical Profile ◽  
Layer Chromatography

The present study was aimed to validate the phenolic, flavonoids, alkaloids and tannins profile of Aspleniuma ethiopicum (Burm. f.) Becherer methanolic extracts using HPTLC (High-performance thin-layer chromatography). The chromatographic separation was performed using the standard method. The separation methodology was optimized and phytoconstituents of A. aethiopicum methanolic extracts were separated. The methanolic extract of A. aethiopicum showed several bands identified as known phenolic compounds. The obtained flavonoids profiles were identified. HPTLC alkaloids profile of A. aethiopicum revealed 11 types of alkaloids. The tannin profile of methanolic extracts of A. aethiopicum demonstrated 11 different types of tannins. The observed profiles will be used as phytomarker to identify the chemical constituents of A. aethiopicum methanolic extracts. These profiles will act as a fingerprint of A. aethiopicum and help us to distinguish from its adulterants. The observed profile will help us to identify the crude drugs and improve the therapeutic potentials of A. aethiopicum.

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

Estimation of Phenolic acid and Flavonoids in leaves of Amaranthus viridis and Amaranthus spinosus

2021 ◽  
pp. 3889-3895
Author(s):  
Swarnakumari S ◽  
Sasikala M ◽  
Mohan S ◽  
Divyanand Maharaj U ◽  
Kavipriya G
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
Methanolic Extract ◽  
Solvent System ◽  
Who Guidelines ◽  
Phytochemical Screening ◽  
Amaranthus Spinosus ◽  
Layer Chromatography

Objective: To explore physiochemical and phytochemical standardization of leaves of Amaranthus viridis and Amaranthus spinosus. Method: As per WHO guidelines, physical standardization parameters with various solvents system were evaluated. The preliminary phytochemical screenings were adopted for disclosing the existence of phytochemicals in the discrete extracts. Thin layer chromatography and HPTLC were employed for methanolic extract of leaves of Amaranthus viridis and Amaranthus spinosus. Result: Preliminary phytochemical screening with various extracts reveals phytoconstituents. HPTLC fingerprint were executed for leaves of Amaranthus viridis and Amaranthus spinosus using selected solvent system. HPTLC analysis implies the presence of phenolic compounds and flavonoids in both the plant Amaranthus viridis and Amaranthus spinosus. Conclusion: Quercetin and Gallic acid was raise to be more in Amaranthus viridis compared to Amaranthus spinosus, however rutin was reported only in Amaranthus spinosus. The outcome of the research leads for isolation, purification and utilization in herbal industries.

Determination of α-, β and γ-Amanitin by High Performance Thin-Layer Chromatography in Amanita phalloides (Vaill. ex Fr.) Seer, from Various Origin

1979 ◽  
Vol 34 (12) ◽  
pp. 1133-1138 ◽  
Author(s):  
Tjakko Stijve ◽  
Ruth Seeger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Dry Weight ◽  
Amanita Phalloides ◽  
Prolonged Storage ◽  
Methanolic Extracts ◽  
Layer Chromatography

A fast, sensitive high performance thin-layer chromatographic method for the determination of α-, β-, and γ-amanitin in crude, methanolic extracts of Amanita phalloides is described. The limit of detection is 50 ng of each amanitin. With this method amanitin was determined in 24 pooled samples of Amanita phalloides, collect­ed between 1970 and 1977 in Germany and Switzerland. The total amanitin content varied be­tween 2010 and 7300 mg/kg dry weight and the average value was 4430 mg/kg of which 43% was α-amanitin, 49% β-amanitin and 8% γ-amanitin. The origin of the fungi hardly influenced their amanitin content: in samples collected during the same year at different sites it fluctuated within a factor of 1.7. The amanitin content of samples from the same site, but collected in different years, maximally varied within a factor of 3.7. The partial decomposition of amanitins during prolonged storage of the lyophilized samples undoubtedly contributed to this variation. Phalloidin, which was determined by conventional thin-layer-chromatography, could not be de­tected in a sample from 1970, whereas its concentration in material collected during 1977 amount­ed to 2400 mg/kg dry weight. The toxicity of the samples (LD50 of lyophilized defatted methanolic extracts intravenously for mice) varied within a factor of 2.5.

scholarly journals LUPEOL VALIDATION AND QUANTIFICATION IN HETEROPOGON CONTORTUS (L.) BEAUV. (SPEAR GRASS) THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2017 ◽  
Vol 10 (12) ◽  
pp. 392 ◽  
Author(s):  
Navjot Kaur ◽  
Raghbir Chand Gupta
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Marker Compound ◽  
Heteropogon Contortus ◽  
Methanolic Extracts ◽  
Validation And Quantification ◽  
Two Samples ◽  
Layer Chromatography

Objective: This is aimed to study the chromatographic evaluation of triterpenoid, i.e., lupeol from methanolic extracts of leaves, stem, and inflorescence of Heteropogon contortus.Methods: The high-performance thin-layer chromatography (HPTLC) densitometry determination of lupeol was performed using optimized mobile phase toluene:methanol:formic acid (7:3:0.3 v/v) with a derivatization of freshly prepared anisaldehyde-sulfuric acid. For densitometry measurements, the plates were scanned at 530 nm absorbance/reflectance wavelength. Quantification of lupeol marker compound in H. contortus leaves, stem, and inflorescence is estimated using 2-12 μg/spot.Results: The appearance of light purple bands on the chromatograms confirmed the lupeol component in plant samples. Further, the confirmation of the compound is done from the densitometric scanning by comparing λmax values. From this, it is reported that lupeol is present in leaf samples, i.e., 10 mg/g of dry wt., while in rest of the two samples, it is found absent.Conclusion: The leaves of H. contortus (spear grass) are a good source of lupeol and can be used as an alternate natural source to synthesize herbal drugs to control cancer and other anti-inflammatory agents. The presently selected HPTLC is validated and most accurate for the quantification and identification of lupeol present in the selected plant. The leaves of the species which are rich in lupeol can be used in pharmaceutical industry.

scholarly journals Detection and Estimation of alpha-Amyrin, beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Saikat S. Mallick ◽  
Vidya V. Dighe
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Pluchea Lanceolata ◽  
Methanolic Extracts ◽  
Whole Plant ◽  
Plant Powders ◽  
Layer Chromatography ◽  
Hptlc Method

A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous estimation of four components, namely, alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. In Ayurveda, both plants have been reported to possess immunomodulatory activity. Chromatographic separation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. was performed on TLC aluminium plates precoated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at λ = 580 nm for α-amyrin, β-sitosterol, and lupeol, and at 366 nm for n-triacontane. The developed HPTLC method has been validated and used for simultaneous quantitation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. The developed HPTLC method is simple, rapid, and precise and can be used for routine quality control.

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