scholarly journals To Develop the Method for UHPLC-HRMS to Determine the Antibacterial Potential of a Central American Medicinal Plant

Separations ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 37 ◽  
Author(s):  
Gaganpreet Kaur Monga ◽  
Anima Ghosal ◽  
Dil Ramanathan
Keyword(s):  
Antibacterial Activity ◽  
Plant Extract ◽  
Bacterial Growth ◽  
Plant Extracts ◽  
Mass Difference ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Liquid Chromatograph ◽  
Aechmea Magdalenae ◽  
Antibacterial Potential

The development of antibiotic resistance by microbials has long been acknowledged. The major challenge worldwide is to develop novel, natural, and potent antibiotics against the multidrug resistant bacteria. In this study, our aim was to develop the method for a highly sensitive instrument, ultra-high performance liquid chromatograph-high resolution mass spectrometer (UHPLC-HRMS), to evaluate the antibacterial property of a natural product. Aechmea magdalenae (Andre) Andre ex Baker, a plant belonging to the family Bromeliaceae, a native of Central America was used in this study. Based on the available literature, it was hypothesized that Aechmea magdalenae has antibacterial activity. In addition, the profiling done on A. magdalenae using gas chromatography-mass spectrometry (GC-MS) also revealed the presence of medicinally important chemical compounds, such as acetic acid. Minimum inhibitory concentration (MIC) of dried Aechmea plant extract was determined for the first time using 96-well plate assay, followed by determination of antibacterial potential using LC-MS. The reason being that other dried methanolic plant extracts, such as Vismia macrophylla, lined up for antibacterial testing have dark extracts, for which determining the antibacterial potential and reading the results with the naked eye would be challenging. To overcome the situation of dark plant extracts, a generalized novel LC-MS method was developed that was used for the plant A. magdalenae, and would be used further for other plants. A blue indicator called resazurin was added to the wells; resazurin, upon incubation with the living cells, got reduced to resorufin (which was pink), while it remained blue with bacterial growth inhibition. The mass difference created due to reduction of resazurin to resorufin was detected by using LTQ Orbitrap Discovery in positive ion mode to determine the antibacterial activity of the plant extract. The sample preparation for LC-MS assay included centrifugation of the samples taken from 96-well plate, followed by filtration of the supernatant, before exposing them to C-18 column. The results obtained from full scan LC-MS spectrum consistently demonstrated the presence of resorufin from wells with bacterial growth, and resazurin from wells with inhibition through peaks of relevant masses.

scholarly journals Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections

2021 ◽  
Vol 5 (1) ◽  
pp. 020-028
Author(s):  
Fernandes Laura Silva ◽  
da Costa Ygor Ferreira Garcia ◽  
de Bessa Martha Eunice ◽  
Ferreira Adriana Lucia Pires ◽  
do Amaral Corrêa José Otávio ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Metabolic Profile ◽  
Bacterial Infections ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
New Antibiotics

Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fight for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profile and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profile and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identified. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and five methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most effective (MIC ≤ 5,000 µg/µL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.

scholarly journals The antimicrobial activity of some medical plants against bacteria that cause urinary tract infection

2018 ◽  
Vol 28 (3) ◽  
pp. 72
Author(s):  
Nehia Hussein ◽  
Noor Ameer Hanon
Keyword(s):  
Urinary Tract Infection ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Tract Infection ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Qualitative Detection

This study was done to evaluate the antibacterial activity of hot ethanolic and aqueous extracts of Syzygium aromatic (S.aromatic ) and Q.infectoria infectoria (gall) against pathogenic bacteria that cause urinary tract infection (UTI). Such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Qualitative detection of the active compounds of the plant extracts was done with chemical reagents. Also, the Qualitative detection of the active groups was further verified with (FTIR). The sensitivity of the UTI causing bacteria was examined against 12 different antibiotics. The results show that E.coli was the most resistant bacteria, resisting 8 from 12 antibiotics; P.aeruginosa resisted 5 from 12 antibiotics; while S.aureus showed resistance to 4 only. The antibacterial activity of the plant extracts was investigated by using four concentrations (40, 60, 80, 100 mg/ml) for each extract against two types of Gram negative bacteria (E.coli, P.aeruginosa), and one type of Gram-positive bacteria (S. aureus). The plant extracts showed different effects on the growth of all bacterial strains. The Minimum Inhibitory Concentration (MIC) and the Minimum bactericidal concentration (MBC) of the ethanolic extracts and aqueous extracts in the study were determined.The kill- time was determined also for each extract.The antioxidant activity of the plants in the study was investigated. Finally, the toxicity of the plant extracts was examined on human red blood cells.

scholarly journals In vitro antibacterial activity of several plant extracts and essential oils against Brucella melitensis

2014 ◽  
Vol 60 (1) ◽  
pp. 29-38
Author(s):  
Mazen Safi ◽  
Ayman Al-Mariri
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Plant Extract ◽  
Plant Extracts ◽  
Brucella Melitensis ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Fluid Medium

Summary Medicinal plants are considered to be new resources for the production of agents that could act as alternatives to antibiotics in the treatment of antibiotic-resistant bacteria. The aim of this study was to evaluate the efficacy of some plants native to Syria in the treatment of brucellosis. In vitro activities of some essential oils and plant extracts of some medicinal plants against 89 Brucella melitensis isolates was determined by disc diffusion method at a concentration of 5%. The microdilution assay in the fluid medium was used to determine the MICs of essential oils and plant extracts. Among the evaluated herbs, only Thymus syriacus and Cinnamomum zeylanicum essential oils and Laurus nobilis plant extract showed a high activity against B. melitensis strains. Thus, minimal inhibitory concentration (MIC50) values for T. syriacus, C. zeylanicum, and L. nobilis against B. melitensis were 6.25, 3.125 and 6.25 μl/ml, respectively. Among studied essential oils and plant extracts, T. syriacus and C. zeylanicum essential oils, and L. nobilis plant extract were the most effective ones. Moreover, T. syriacus - C. zeylanicum combination was more effective than use of each of them alone. Then, T. syriacus and C. zeylanicum essential oils and L. nobilis plant extract could act as bactericidal agents against B. melitensis.

scholarly journals In vitro Study of Antibacterial Activity on Multi-Resistant Bacteria and Chemical Composition of the Chloroform Extract of Endemic Centaurea drabifolia subsp. cappadocica

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aysel Ugur ◽  
Nurdan Sarac ◽  
M. Emin Duru ◽  
Yavuz Beyatli
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
In Vitro Study ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Ethyl Palmitate

The antimicrobial activity of the n-hexane, chloroform, ethyl acetate and ethanol extracts of the aerial parts of C. drabifolia S.M. subsp. cappadocica (DC.) Wagenitz (Asteraceae) was evaluated against microorganisms including multi-antibiotic resistant bacteria using the paper disc diffusion method. The chemical composition of the chloroform extract of this plant was determined by gas chromatography and gas chromatography-mass spectrometry. The chloroform extract exhibited significant antibacterial activity against all the bacteria tested, except Stenotrophomonas maltophilia MU63. The major compounds of the chloroform extract were spathulenol (14.1%), caryophyllene oxide (12.5%), octadecanol (10.2%), ethyl palmitate (7.7%), [Z,Z]-10,12-hexadecadienal (6.0%), 3-hydroxy p-anisaldehyde (5.9%) and pentacosane (5.8%).

scholarly journals GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica

2020 ◽  
pp. 17-23
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Dioctyl Phthalate ◽  
Gas Chromatography Mass Spectrometry ◽  
Antimicrobial Compounds ◽  
E Coli

Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartii and 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds.

scholarly journals Antibacterial activity of Lamiaceae plant extracts in clinical isolates of multidrug-resistant bacteria

2018 ◽  
Vol 90 (2) ◽  
pp. 1665-1670 ◽  
Author(s):  
FELIPE V. DE ASSIS ◽  
FLÁVIA L. SIQUEIRA ◽  
ISABELA E. GONÇALVES ◽  
RAFAEL P. LACERDA ◽  
RAFAELA A. NASCIMENTO ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

scholarly journals Parkia speciosaHassk.: A Potential Phytomedicine

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yusof Kamisah ◽  
Faizah Othman ◽  
Hj Mohd Saad Qodriyah ◽  
Kamsiah Jaarin
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Southeast Asia ◽  
Plant Extract ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Pharmacological Properties ◽  
Total Polyphenol ◽  
Ongoing Research

Parkia speciosaHassk., or stink bean, is a plant indigenous to Southeast Asia. It is consumed either raw or cooked. It has been used in folk medicine to treat diabetes, hypertension, and kidney problems. It contains minerals and vitamins. It displays many beneficial properties. Its extracts from the empty pods and seeds have a high content of total polyphenol, phytosterol, and flavonoids. It demonstrates a good antioxidant activity. Its hypoglycemic effect is reported to be attributable to the presence ofβ-sitosterol, stigmasterol, and stigmast-4-en-3-one. The cyclic polysulfide compounds exhibit antibacterial activity, while thiazolidine-4-carboxylic acid possesses anticancer property. The pharmacological properties of the plant extract are described in this review. With ongoing research conducted on the plant extracts,Parkia speciosahas a potential to be developed as a phytomedicine.

scholarly journals Antibacterial Activity of Rosmarinus officinalis against Multidrug-Resistant Clinical Isolates and Meat-Borne Pathogens

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Aseer Manilal ◽  
Kuzhunellil Raghavanpillai Sabu ◽  
Melat Woldemariam ◽  
Addis Aklilu ◽  
Gelila Biresaw ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Plant Extract ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Chemical Constituents ◽  
Multidrug Resistant ◽  
Rosmarinus Officinalis ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Crude Plant Extract

Background. In developing countries, the prevalence of bacterial infections is quite rampant due to several factors such as the HIV/AIDS pandemic, lack of hygiene, overcrowding, and resistance to conventional antimicrobials. Hence the use of plant-based antimicrobial agents could provide a low-cost alternative therapy. Rosmarinus officinalis is reputed as a medicinal plant in Ethiopia; however, its antibacterial activity against many of the clinical isolates remains overlooked. Methods. Tender foliage of R. officinalis was collected and extracted in ethanol (EtOH) and evaluated for their antimicrobial activity against ten multidrug-resistant (MDR) clinical isolates, human type culture pathogens, and meat-borne bacterial isolates by employing agar well diffusion assay. Results. EtOH extract of R. officinalis efficiently subdued the growth of all tested MDR clinical isolates in varying degrees. Salmonella sp. and Staphylococcus aureus were found to be the most sensitive clinical isolates. Likewise, it efficiently repressed the growth of meat-borne pathogens, particularly, S. aureus and Salmonella sp. showing its potentiality to be used as a natural antibacterial agent in the meat processing industry. The mechanism of antibiosis of plant extract against meat-borne pathogens is inferred to be bactericidal. Chemical constituents of the crude plant extract were analysed by Gas Chromatography-Mass Spectroscopy (GC-MS), Fourier Transform Infrared (FT-IR), and UV-visible spectroscopy showing genkwanin (26%), camphor (13%), endo-borneol (13%), alpha-terpineol (12%), and hydroxyhydrocaffeic acid (13%) as the major compounds. Conclusion. Overall results of the present study conclude that R. officinalis could be an excellent source of antimicrobial agents for the management of drug-resistant bacteria as well as meat-borne pathogens.

scholarly journals GC-MS and Antibacterial Potential of Methanolic Extract Hyphaene Thebaica L. Fruit Pulp against Antibiotics-resistant Pathogens

2021 ◽  
Vol 15 (3) ◽  
pp. 1655-1664
Author(s):  
Emad M. Abdallah ◽  
Adil A.H. Mujawah ◽  
Samiah H. Al-Mijalli
Keyword(s):  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Bioactive Molecules ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Minimal Bactericidal Concentration ◽  
Hexadecenoic Acid ◽  
Bacterial Strains ◽  
Antibacterial Potential

Methanol extract obtained from the fruits of Hyphaene thebaica (doum fruit) was chemically analyzed using GC-MS (gas chromatography-mass spectrometry). Up to thirty compounds were identified in the extract. Acetic acid decyl ester (36.80%), n-Hexadecenoic acid (5.14%),1H-Purine-2,6-dione, 3,7-dihydro-1-methyl (4.24%), 2-Furancarboxaldehyde, 5-(2-hydroxy-2-phenylacetyl)-dimethylhydrazone (4.67%), Propanoic acid 3,3′-dithiobis (3.52%) and [1,2,4] Triazolo[1,5-a]pyrimidin-7-ol were major components. The antibacterial potential of the extract against six clinical bacterial isolates resistant to antibiotics was also investigated, using various in vitro assays including well diffusion, minimal inhibitory and minimal bactericidal concentration. It was found that, the methanol extract of doum fruit was characterized by antibacterial action toward one Gram-positive ß-lactamase bacteria (Staphylococcus aureus), and one Gram-negative Multidrug-resistant bacteria (Proteus mirabilis). The other four bacterial strains showed no susceptibility towards the extract. The study suggests future additional biochemical and microbiological investigations in order to understand the mechanism of action of the bioactive molecules as antimicrobial agents.

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